##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6835828
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18029886065009	33.0	31.0	34.0	30.0	34.0
2	32.39928081865138	34.0	31.0	34.0	31.0	34.0
3	32.45544402228962	34.0	31.0	34.0	31.0	34.0
4	36.08381998493818	37.0	35.0	37.0	35.0	37.0
5	35.93785595541608	37.0	35.0	37.0	35.0	37.0
6	35.957357762658745	37.0	35.0	37.0	35.0	37.0
7	35.90727282781252	37.0	35.0	37.0	35.0	37.0
8	35.90972256762458	37.0	35.0	37.0	35.0	37.0
9	37.69160809780468	39.0	37.0	39.0	35.0	39.0
10-11	37.58149458997505	39.0	37.0	39.0	35.0	39.0
12-13	37.54869571030751	39.0	37.0	39.0	35.0	39.0
14-15	38.84320107234998	40.0	38.0	41.0	35.0	41.0
16-17	38.831803550352646	40.0	38.0	41.0	35.0	41.0
18-19	38.80139677300248	40.0	38.0	41.0	34.5	41.0
20-21	38.78983288877791	40.0	38.0	41.0	34.0	41.0
22-23	38.71801251434715	40.0	38.0	41.0	34.0	41.0
24-25	38.66591607998069	40.0	38.0	41.0	34.0	41.0
26-27	38.504192528837876	40.0	38.0	41.0	34.0	41.0
28-29	38.38196267026248	40.0	38.0	41.0	34.0	41.0
30-31	38.26875839700688	40.0	38.0	41.0	34.0	41.0
32-33	38.18936053752447	40.0	38.0	41.0	34.0	41.0
34-35	38.23583125482505	40.0	38.0	41.0	34.0	41.0
36-37	38.19706374934546	40.0	38.0	41.0	33.0	41.0
38-39	38.14015859950635	40.0	38.0	41.0	33.0	41.0
40-41	38.072067109889204	40.0	37.5	41.0	33.0	41.0
42-43	38.04201348270466	40.0	37.0	41.0	33.0	41.0
44-45	38.08942818059272	40.0	37.5	41.0	33.0	41.0
46-47	38.08029307601129	40.0	37.0	41.0	33.0	41.0
48-49	38.00720496577131	40.0	37.0	41.0	33.0	41.0
50-51	37.92162279696507	40.0	37.0	41.0	33.0	41.0
52-53	37.69463713476094	40.0	37.0	41.0	32.5	41.0
54-55	37.58194966399349	40.0	36.0	41.0	32.0	41.0
56-57	37.517339077300036	39.0	36.0	41.0	32.0	41.0
58-59	37.36303355611816	39.0	36.0	41.0	32.0	41.0
60-61	37.16582637322295	39.0	35.0	41.0	31.5	41.0
62-63	36.92477396603523	39.0	35.0	40.0	31.0	41.0
64-65	36.643037809106204	38.0	35.0	40.0	31.0	41.0
66-67	36.37037393499389	37.5	35.0	40.0	31.0	41.0
68-69	36.08073531989055	37.0	35.0	40.0	30.5	41.0
70-71	35.78337127898591	36.5	34.0	39.0	30.0	41.0
72-73	35.4692022515153	36.0	34.0	39.0	30.0	41.0
74-75	35.090592421892026	35.5	34.0	39.0	30.0	40.0
76-77	33.71631729212417	34.5	32.0	36.5	28.0	39.0
78-79	34.3815374170549	35.0	33.5	37.0	29.0	39.0
80-81	34.29891432902089	35.0	34.0	37.0	29.5	39.0
82-83	34.011472414671665	35.0	33.5	36.0	29.0	38.5
84-85	33.85743891477551	35.0	33.0	36.0	29.0	37.0
86-87	33.62907452427019	35.0	33.0	35.0	29.0	37.0
88-89	33.52665323173483	35.0	33.0	35.0	29.0	36.5
90-91	33.37236682288458	35.0	33.0	35.0	29.0	36.0
92-93	33.27797830382775	35.0	33.0	35.0	29.0	36.0
94-95	33.23687249717111	35.0	33.0	35.0	29.0	36.0
96-97	33.250622741304255	35.0	33.0	35.0	29.0	35.0
98-99	33.341552455467536	35.0	33.0	35.0	29.5	35.0
100	32.13120242545794	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	42.0
21	174.0
22	871.0
23	3196.0
24	8527.0
25	18533.0
26	33848.0
27	54185.0
28	78418.0
29	106102.0
30	135931.0
31	168888.0
32	211218.0
33	267232.0
34	358696.0
35	495880.0
36	766299.0
37	1336250.0
38	1780169.0
39	967878.0
40	43485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06812985932355	20.18191505111012	13.305352328935133	24.44460276063119
2	15.35064954823322	21.26039449793061	39.877422310801265	23.511533643034905
3	18.61040096386275	25.39587011258914	30.327708655045154	25.666020268502955
4	12.807943675586921	17.339099228359753	38.29239120703446	31.56056588901886
5	13.263279883578113	37.72425227785134	35.77933792365753	13.233129914913015
6	30.98537587546088	37.41865652558841	18.25413102845771	13.341836570492996
7	27.021364493079698	32.21827699585186	23.009399885427193	17.750958625641253
8	24.838278552356787	36.59378205537062	21.026026400898328	17.54191299137427
9	25.362882155607192	16.450604081904928	21.291466081358397	36.89504768112948
10-11	24.487245729412734	26.581308072701653	28.835848415144444	20.09559778274117
12-13	24.843552236832174	24.053669869985026	29.140273862946813	21.962504030235984
14-15	21.870620501276512	25.716379932321292	26.426842512713893	25.986157053688302
16-17	21.868862224466636	28.778916758414812	27.231996175396905	22.120224841721644
18-19	21.87529257628634	28.188547569393247	28.63226876525785	21.303891089062564
20-21	22.44876656989564	27.367791757548748	28.55899044868582	21.62445122386979
22-23	22.244682153923545	27.27152213810356	28.429905918491954	22.053889789480937
24-25	22.07539403332722	27.38772835492252	28.489093444358215	22.047784167392038
26-27	22.04280239543932	27.539205289993173	28.400076820644514	22.01791549392299
28-29	21.988679085178866	27.65260251559764	28.37626789094597	21.982450508277523
30-31	22.224153284332814	27.48776602399745	28.3218820615931	21.96619863007664
32-33	22.000789164189044	27.635906410492435	28.318045549542898	22.045258875775623
34-35	22.161897429925304	27.658157929906192	28.26994748194553	21.909997158222975
36-37	22.135258108114762	27.556139471673614	28.268217419502207	22.040385000709424
38-39	21.93549570423887	27.633056440770183	28.256226324152372	22.175221530838567
40-41	22.375523494717267	27.41065253107773	28.280333211615932	21.933490762589074
42-43	21.95631158455597	27.541599133988665	28.161253100146272	22.340836181309097
44-45	22.0370496816112	27.60059370882862	28.08260430187014	22.279752307690043
46-47	22.065890884617186	27.555735371489188	28.192261269027135	22.186112474866487
48-49	21.975832808764036	27.901992112216583	27.946102055155958	22.176073023863424
50-51	21.952062820806688	28.006761890290228	27.614622797009368	22.426552491893716
52-53	21.927464026889247	27.769115680435913	27.568580742844627	22.734839549830212
54-55	22.162481329678815	27.374718008468868	28.083917152115916	22.3788835097364
56-57	21.868433408258973	26.893948844701086	28.317419870771904	22.920197876268038
58-59	21.66468685680682	26.680655790893145	28.267410193756398	23.387247158543634
60-61	21.93885334733322	27.047894992984904	28.728249955963737	22.285001703718134
62-63	21.373841561608646	27.329502311717164	28.685359384346633	22.61129674232756
64-65	21.012132292676313	27.887637832834383	28.1910584008044	22.9091714736849
66-67	21.058985199030882	28.7533627073303	27.546608336820018	22.641043756818796
68-69	21.297632915707855	28.791086543926664	27.216881536194563	22.69439900417091
70-71	21.299817928401474	28.09660153395715	27.187370243634962	23.416210294006415
72-73	21.624388550029206	27.27201191127513	27.191914764385245	23.91168477431042
74-75	21.742884526066582	27.30288672502516	27.529768670812942	23.424460078095315
76-77	22.224783486559254	27.220442719186483	26.55938806951154	23.995385724742718
78-79	22.520515708735502	27.240670005185546	25.934460133371807	24.30435415270715
80-81	22.230072771695237	27.848744376447904	26.25675952878752	23.66442332306934
82-83	22.20578616161276	27.52255083275742	26.28039447177903	23.991268533850796
84-85	22.258427490603516	28.151063427531493	26.212248695460584	23.378260386404406
86-87	23.099571402556936	27.892451773633763	26.340371496683364	22.667605327125937
88-89	22.550492469852927	28.15679990921008	26.891990745618187	22.40071687531881
90-91	22.531343034097354	27.58957164166781	26.592507549722992	23.286577774511844
92-93	22.5127058374459	26.837431092734924	27.029478859115336	23.620384210703836
94-95	21.398541602087906	27.565362278054195	28.02241457260031	23.013681547257587
96-97	21.46387516085891	27.305502246223384	28.39734068910375	22.833281903813955
98-99	22.771153970928246	29.205505499585925	28.69505112683991	19.32828940264592
100	29.35072028166608	40.966346908808404	29.682932809525514	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1781.0
1	1484.5
2	1725.5
3	2452.0
4	3107.0
5	4641.5
6	6219.5
7	7546.5
8	8538.5
9	8623.0
10	7977.5
11	7252.5
12	7421.5
13	8981.5
14	13010.0
15	18775.0
16	23740.5
17	26822.5
18	28263.0
19	28542.0
20	28123.0
21	27996.0
22	29019.5
23	32550.0
24	39092.0
25	48122.5
26	60078.0
27	74038.5
28	89027.0
29	105215.0
30	120318.5
31	133639.0
32	147517.5
33	161036.5
34	173877.5
35	187386.0
36	202598.0
37	217416.0
38	229896.5
39	241006.0
40	252158.0
41	262428.5
42	269823.0
43	275662.0
44	281714.5
45	288410.0
46	293957.5
47	296894.0
48	297634.0
49	296978.0
50	294555.5
51	288242.5
52	277576.0
53	265172.5
54	251038.5
55	233511.0
56	214688.0
57	195042.5
58	173351.0
59	149384.5
60	124745.5
61	101566.0
62	81468.0
63	63744.5
64	48214.5
65	36907.0
66	28367.5
67	22172.0
68	17237.0
69	12633.5
70	9253.5
71	6859.5
72	4952.5
73	3442.5
74	2312.5
75	1592.5
76	1120.0
77	710.0
78	455.5
79	322.0
80	229.5
81	149.0
82	101.5
83	76.0
84	49.5
85	28.0
86	17.0
87	10.5
88	6.0
89	3.5
90	3.5
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0012580772950987066
18-19	5.8515223027846806E-5
20-21	0.0
22-23	0.007000863147171091
24-25	0.007712312311477318
26-27	0.021111700882043057
28-29	0.01609644798209741
30-31	0.0018208923312238395
32-33	0.005873430303643082
34-35	0.03282967103158854
36-37	0.0053558140270410426
38-39	0.0025256606369024577
40-41	0.007525297846059688
42-43	0.007719612640865699
44-45	0.00886562952750612
46-47	0.0053858130304800115
48-49	0.001033753975778031
50-51	0.00262368881956055
52-53	9.534186319816201E-4
54-55	0.005307223090174332
56-57	8.343602850336744E-4
58-59	0.0013139978815434154
60-61	7.779474340099947E-4
62-63	0.0013841681663203207
64-65	4.110577211446498E-4
66-67	0.0057534436102187276
68-69	0.004238710749603073
70-71	0.0016070534273645914
72-73	0.0015401358063429696
74-75	8.250510612443473E-4
76-77	9.180472825255062E-4
78-79	8.662466353460145E-4
80-81	0.0012621803913825055
82-83	0.007730980838550559
84-85	8.178109605911329E-4
86-87	6.834901585228489E-5
88-89	5.158491680840946E-4
90-91	3.028157015190043E-4
92-93	0.002580999422617061
94-95	0.003831278473484299
96-97	6.475905906382361E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	185.0
20-21	553.0
22-23	1201.0
24-25	2686.0
26-27	4531.0
28-29	11220.0
30-31	23586.0
32-33	30948.0
34-35	43049.0
36-37	63792.0
38-39	38990.0
40-41	68140.0
42-43	74848.0
44-45	82606.0
46-47	82209.0
48-49	76778.0
50-51	40181.0
52-53	8102.0
54-55	7865.0
56-57	7847.0
58-59	41698.0
60-61	74529.0
62-63	71649.0
64-65	75335.0
66-67	79754.0
68-69	79197.0
70-71	77113.0
72-73	73530.0
74-75	74768.0
76-77	73689.0
78-79	77864.0
80-81	77523.0
82-83	74526.0
84-85	74986.0
86-87	79134.0
88-89	85313.0
90-91	90028.0
92-93	95435.0
94-95	115208.0
96-97	279635.0
98-99	1491733.0
100-101	2903864.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.49222633801014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.01478797429003	79.63086429552587
2	6.5234238067594585	11.414977443995243
3	1.4474535426110062	3.7992279889163023
4	0.4878321438661806	1.7072608138438639
5	0.21448371070169786	0.938282868126462
6	0.10836440713949375	0.5688625941859721
7	0.06740788093365604	0.4128365902929152
8	0.03636811546369535	0.2545541911709216
9	0.026623580681617298	0.20964207122319015
>10	0.07197697048121125	0.9661545724408114
>50	0.0011306996115719378	0.06444640602887557
>100	1.471674603940091E-4	0.03289016424963378
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4628805756961703E-5	0.0	1.4628805756961703E-5	0.0
3	0.0	1.4628805756961703E-5	0.0	1.4628805756961703E-5	0.0
4	0.0	5.851522302784681E-5	0.0	4.388641727088511E-5	0.0
5	0.0	5.851522302784681E-5	0.0	4.388641727088511E-5	0.0
6	0.0	5.851522302784681E-5	0.0	4.388641727088511E-5	0.0
7	0.0	5.851522302784681E-5	0.0	5.851522302784681E-5	0.0
8	0.0	7.314402878480851E-5	0.0	5.851522302784681E-5	0.0
9	0.0	7.314402878480851E-5	0.0	1.4628805756961702E-4	0.0
10-11	0.0	7.314402878480851E-5	0.0	1.8286007196202127E-4	0.0
12-13	0.0	7.314402878480851E-5	0.0	1.9017447484050214E-4	7.3144028784808516E-6
14-15	0.0	1.3897365469113618E-4	0.0	1.9017447484050214E-4	2.1943208635442554E-5
16-17	0.0	1.4628805756961702E-4	0.0	2.1211768347594468E-4	2.9257611513923406E-5
18-19	0.0	1.6091686332657873E-4	0.0	2.4868969786834896E-4	7.314402878480851E-5
20-21	0.0	1.6091686332657873E-4	0.0	3.2914812953163826E-4	7.314402878480851E-5
22-23	0.0	1.9017447484050214E-4	0.0	4.2423536695188936E-4	7.314402878480851E-5
24-25	0.0	1.9017447484050214E-4	0.0	7.021826763341616E-4	7.314402878480851E-5
26-27	0.0	1.9017447484050214E-4	0.0	0.0010313308058657999	7.314402878480851E-5
28-29	0.0	1.9017447484050214E-4	0.0	0.0025454122017113364	7.314402878480851E-5
30-31	0.0	2.3406089211138725E-4	0.0	0.007526520561956795	7.314402878480851E-5
32-33	7.3144028784808516E-6	2.560041007468298E-4	0.0	0.015499219699500924	8.777283454177022E-5
34-35	1.4628805756961703E-5	3.072049208961957E-4	0.0	0.02587835738406525	8.777283454177022E-5
36-37	1.4628805756961703E-5	3.3646253241011913E-4	0.0	0.04868466555916855	8.777283454177022E-5
38-39	1.4628805756961703E-5	3.5109133816708086E-4	0.0	0.31981495145869676	8.777283454177022E-5
40-41	1.4628805756961703E-5	3.9497775543796595E-4	0.0	0.599144682985002	8.777283454177022E-5
42-43	1.4628805756961703E-5	4.022921583164468E-4	0.0	0.6438503133782769	8.777283454177022E-5
44-45	1.4628805756961703E-5	4.461785755873319E-4	0.0	0.7029141166220099	8.777283454177022E-5
46-47	1.4628805756961703E-5	4.827505899797362E-4	0.0	0.7656716933193755	1.0240164029873192E-4
48-49	1.4628805756961703E-5	4.90064992858217E-4	0.0	0.8241430299299515	1.0240164029873192E-4
50-51	1.4628805756961703E-5	5.851522302784681E-4	0.0	0.8799665526985172	1.0240164029873192E-4
52-53	1.4628805756961703E-5	5.851522302784681E-4	0.0	1.5902535874220358	1.2434484893417448E-4
54-55	1.4628805756961703E-5	6.070954389139106E-4	0.0	2.5465386197546223	1.4628805756961702E-4
56-57	1.4628805756961703E-5	6.290386475493532E-4	0.0	3.572186134583843	1.4628805756961702E-4
58-59	1.4628805756961703E-5	6.290386475493532E-4	0.0	4.6144080863357	1.4628805756961702E-4
60-61	1.4628805756961703E-5	6.582962590632766E-4	0.0	4.89054142380411	1.4628805756961702E-4
62-63	1.4628805756961703E-5	6.582962590632766E-4	0.0	4.901578857747737	1.4628805756961702E-4
64-65	1.4628805756961703E-5	6.875538705772E-4	0.0	4.911255812755968	1.4628805756961702E-4
66-67	1.4628805756961703E-5	6.875538705772E-4	0.0	4.919345542339567	1.4628805756961702E-4
68-69	1.4628805756961703E-5	6.948682734556808E-4	0.0	4.926564857980628	1.4628805756961702E-4
70-71	1.4628805756961703E-5	7.021826763341617E-4	0.0	4.932672384384159	1.4628805756961702E-4
72-73	1.4628805756961703E-5	7.021826763341617E-4	0.0	4.938085042514235	1.9017447484050214E-4
74-75	1.4628805756961703E-5	7.168114820911235E-4	0.0	4.942268880960726	1.9017447484050214E-4
76-77	1.4628805756961703E-5	7.387546907265659E-4	0.0	4.9462406017237415	1.9017447484050214E-4
78-79	1.4628805756961703E-5	7.460690936050468E-4	0.0	4.949063961234835	1.9017447484050214E-4
80-81	1.4628805756961703E-5	7.460690936050468E-4	0.0	4.9498685455514675	1.9017447484050214E-4
82-83	1.4628805756961703E-5	7.460690936050468E-4	0.0	4.950314724127056	1.9017447484050214E-4
84-85	1.4628805756961703E-5	7.533834964835277E-4	0.0	4.950490269796139	1.9017447484050214E-4
86-87	1.4628805756961703E-5	7.753267051189703E-4	0.0	4.950607300242194	1.9017447484050214E-4
88	1.4628805756961703E-5	8.045843166328936E-4	0.0	4.950636557853708	2.0480328059746384E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	9055	0.0	62.58662	94
TATCTCG	11625	0.0	50.097424	94
GGTATCA	6240	0.0	44.90394	1
CCGTCTT	4965	0.0	41.0481	90-91
GCCGTCT	5190	0.0	40.512356	90-91
TGCCGTC	5005	0.0	39.950134	88-89
TATGCCG	5085	0.0	38.687458	86-87
ATGCCGT	5335	0.0	38.37535	88-89
TCGTATG	5820	0.0	34.94799	84-85
CGTATGC	5660	0.0	34.51473	84-85
CTCGTAT	5675	0.0	34.06124	82-83
TCTCGTA	5895	0.0	33.699593	82-83
GTATCAA	10870	0.0	31.505735	1
GTATGCC	6835	0.0	30.32401	86-87
CTATCTC	13990	0.0	29.167116	92-93
TCAACGC	12170	0.0	27.11701	4
ATCAACG	12260	0.0	26.884087	3
CAACGCA	12500	0.0	26.434328	5
ACGCTAT	24205	0.0	26.082308	94
CGCTATC	14485	0.0	25.8668	92-93
>>END_MODULE