FastQCFastQC Report
Fri 27 May 2016
SRR2140230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694393
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.042.3578531
GTATCAA94350.033.7500151
TCAACGC112650.027.3624784
ATCAACG113400.027.2534183
CAACGCA115950.026.5837275
TATCAAC120950.025.7881622
AACGCAG120750.025.628286
GTACATG147950.022.4047661
TACATGG147850.022.171732
ACGCAGA142550.021.6803787
ACATGGG149000.021.480693
CGCAGAG146350.021.0617228
CATGGGG118200.019.5237754
GCAGAGT176800.017.3881729
ACCGAAC7400.017.0802738
AGAGTAC155700.016.969410-11
GGACCGA9750.016.3089076
GTGGTAT31800.016.2832621
GAGTACA104700.016.2777521
GAGTACT104000.016.27027312-13
Produced by FastQC (version 0.11.5)