##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5694393
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15925332164464	33.0	31.0	34.0	30.0	34.0
2	32.37747166379279	34.0	31.0	34.0	31.0	34.0
3	32.42789933887598	34.0	31.0	34.0	30.0	34.0
4	36.06353618375128	37.0	35.0	37.0	35.0	37.0
5	35.91840078477197	37.0	35.0	37.0	35.0	37.0
6	35.94394029354841	37.0	35.0	37.0	35.0	37.0
7	35.8944273779488	37.0	35.0	37.0	35.0	37.0
8	35.896206847683324	37.0	35.0	37.0	35.0	37.0
9	37.676549370582606	39.0	37.0	39.0	35.0	39.0
10-11	37.563479724704635	39.0	37.0	39.0	35.0	39.0
12-13	37.525706076134895	39.0	37.0	39.0	35.0	39.0
14-15	38.80695765114912	40.0	38.0	41.0	35.0	41.0
16-17	38.793164258244914	40.0	38.0	41.0	35.0	41.0
18-19	38.766386689503165	40.0	38.0	41.0	34.0	41.0
20-21	38.751983195307275	40.0	38.0	41.0	34.0	41.0
22-23	38.67557082781725	40.0	38.0	41.0	34.0	41.0
24-25	38.61398027331167	40.0	38.0	41.0	34.0	41.0
26-27	38.445209282549406	40.0	38.0	41.0	34.0	41.0
28-29	38.31486049355229	40.0	38.0	41.0	34.0	41.0
30-31	38.19302928908044	40.0	38.0	41.0	34.0	41.0
32-33	38.11434266353051	40.0	37.5	41.0	33.0	41.0
34-35	38.16906322776141	40.0	38.0	41.0	33.0	41.0
36-37	38.129542959671895	40.0	38.0	41.0	33.0	41.0
38-39	38.068752809891976	40.0	37.5	41.0	33.0	41.0
40-41	38.00093095730961	40.0	37.0	41.0	33.0	41.0
42-43	37.9695282094122	40.0	37.0	41.0	33.0	41.0
44-45	38.006873155089245	40.0	37.0	41.0	33.0	41.0
46-47	37.989970220269555	40.0	37.0	41.0	33.0	41.0
48-49	37.907157562513405	40.0	37.0	41.0	33.0	41.0
50-51	37.81939142605867	40.0	37.0	41.0	33.0	41.0
52-53	37.583861274911	40.0	36.5	41.0	32.0	41.0
54-55	37.45311798820153	39.0	36.0	41.0	32.0	41.0
56-57	37.37878200083978	39.0	36.0	41.0	32.0	41.0
58-59	37.20663500141912	39.0	35.5	41.0	31.5	41.0
60-61	37.00021359722465	39.0	35.0	40.5	31.0	41.0
62-63	36.740140348842324	38.0	35.0	40.0	31.0	41.0
64-65	36.438002867034186	38.0	35.0	40.0	31.0	41.0
66-67	36.145998287143016	37.0	35.0	40.0	30.0	41.0
68-69	35.85483441412184	37.0	34.0	39.5	30.0	41.0
70-71	35.547879807021545	36.0	34.0	39.0	30.0	41.0
72-73	35.236692704503184	36.0	34.0	39.0	30.0	40.0
74-75	34.88498127408421	35.5	34.0	38.5	29.0	40.0
76-77	33.548469243706826	34.5	32.0	36.5	28.0	39.0
78-79	34.27400576894929	35.0	33.5	37.0	29.0	39.0
80-81	34.22354245576511	35.0	34.0	36.5	29.0	39.0
82-83	33.955095506664065	35.0	33.5	36.0	29.0	38.0
84-85	33.78388428567121	35.0	33.0	36.0	29.0	37.0
86-87	33.562098691996226	35.0	33.0	35.0	29.0	37.0
88-89	33.456581260506574	35.0	33.0	35.0	29.0	36.5
90-91	33.284068358207094	35.0	33.0	35.0	29.0	36.0
92-93	33.18259565666017	35.0	33.0	35.0	29.0	36.0
94-95	33.179155081234654	35.0	33.0	35.0	29.0	36.0
96-97	33.21456245268896	35.0	33.0	35.0	29.0	35.0
98-99	33.31198594731309	35.0	33.0	35.0	29.5	35.0
100	31.995502952816036	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	3.0
20	30.0
21	171.0
22	774.0
23	2801.0
24	7280.0
25	15971.0
26	29048.0
27	46832.0
28	66598.0
29	90779.0
30	115838.0
31	144738.0
32	179757.0
33	229004.0
34	305460.0
35	422820.0
36	651374.0
37	1120428.0
38	1416779.0
39	814740.0
40	33167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4955390328697	19.89817000688221	13.02619963181326	24.58009132843483
2	15.638067130245489	21.260843780891133	39.562829611514346	23.538259477349037
3	18.708771944612884	25.352008545950376	30.230948232761595	25.70827127667514
4	12.831077869054699	17.313364216344045	38.21469645667238	31.640861457928875
5	13.2901610408695	37.70092791277314	35.69692151560316	13.3119895307542
6	31.030682286944366	37.332881660960176	18.26610843333082	13.370327618764636
7	27.204076009506196	32.113853048077296	22.920792435646785	17.761278506769727
8	25.052204861870266	36.209636391446814	21.013653255052823	17.7245054916301
9	25.68874329537845	16.040603449744335	21.097033520517464	37.17361973435975
10-11	24.651714414512664	26.343738480993494	28.81701350784886	20.18753359664498
12-13	25.087177860748284	23.90368033959019	29.015524569519524	21.993617230142
14-15	22.115096376382873	25.440455198648916	26.4360924860648	26.00835593890341
16-17	22.018411699811427	28.43683189932975	27.21502537443492	22.3297310264239
18-19	22.02353528752137	27.86742309596005	28.595262825110808	21.51377879140778
20-21	22.710911933203416	27.050506890057196	28.55506851860324	21.68351265813615
22-23	22.436723359779855	27.03375635295685	28.32991719145626	22.19960309580704
24-25	22.25571708496546	27.081392955672555	28.470603075306254	22.192286884055722
26-27	22.213828361743317	27.245111848142056	28.345511217594787	22.195548572519836
28-29	22.17192461297128	27.377599665285775	28.3342400855287	22.116235636214242
30-31	22.393314794642073	27.246801723367074	28.23722242068675	22.122661061304104
32-33	22.154048031550918	27.380712406588714	28.23301798681111	22.232221575049255
34-35	22.29629939604877	27.358918788963038	28.26573854154534	22.07904327344286
36-37	22.30716621244634	27.33834340916708	28.184761733746882	22.169728644639694
38-39	22.16605981962411	27.468798300220428	28.26162493575769	22.10351694439778
40-41	22.400661150813583	27.306108685831422	28.09238818294162	22.20084198041338
42-43	22.244431337492742	27.42744841441724	27.978075551336758	22.350044696753265
44-45	22.308050616102165	27.526578396068807	27.854140429422763	22.311230558406262
46-47	22.36053706468209	27.48884295572805	27.701748540725774	22.44887143886408
48-49	22.313154045869783	27.614128878519097	27.6580050062413	22.41471206936982
50-51	22.304170836444527	27.67870144597052	27.583603400679763	22.43352431690518
52-53	22.429486801184257	27.587762682725863	27.474547346628107	22.50820316946177
54-55	22.367197796006323	27.538870102028795	27.570687010756906	22.523245091207976
56-57	22.36526819351421	27.61366858469262	27.565136599672375	22.4559266221208
58-59	22.336407893727728	27.66865260378554	27.525499791248148	22.46943971123859
60-61	22.39689026708193	27.62485493371995	27.579036703499344	22.399218095698778
62-63	22.3531190444517	27.697103939370482	27.555753219199875	22.394023796977947
64-65	22.28848099451511	27.676137864121507	27.51140738609546	22.523973755267924
66-67	22.38531891514511	27.736491297895398	27.336743203862273	22.541446583097223
68-69	22.328654314741772	27.72069222495813	27.43380852982024	22.516844930479863
70-71	22.292584959216363	27.517023645296618	27.51637886199244	22.67401253349458
72-73	22.39372065487194	27.514656641926027	27.441730641214928	22.6498920619871
74-75	22.23973280182166	27.64632374707651	27.425102435768505	22.688841015333328
76-77	22.449213112192094	27.599212765277414	27.310375012497463	22.641199110033032
78-79	22.42809211102234	27.53325322134725	27.318844666595847	22.71981000103456
80-81	22.3778920699633	27.599307754845213	27.339072079687316	22.683728095504176
82-83	22.446927812321064	27.576154282958214	27.4778266953995	22.49909120932122
84-85	22.549233233752467	27.561403246590153	27.37241733719444	22.516946182462938
86-87	22.381554168299846	27.572035597536814	27.486851249478534	22.559558984684806
88-89	22.45372593611828	27.527301635485536	27.487565283815087	22.5314071445811
90-91	22.46736713944947	27.519720121772572	27.517920701844734	22.49499203693322
92-93	22.556751950747344	27.45051085155711	27.579948979459967	22.41278821823558
94-95	22.325114338396208	27.508868912889856	27.679384026537313	22.486632722176623
96-97	22.364686371026473	27.647124821251168	27.745585422391216	22.24260338533114
98-99	23.45735857035977	29.61279056708538	27.91909978880084	19.010751073754015
100	30.13636827765998	40.97490992875047	28.88872179358955	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	914.0
1	859.0
2	1321.0
3	2001.0
4	2640.0
5	4145.5
6	5622.0
7	6771.5
8	7624.0
9	7692.0
10	7070.5
11	6202.0
12	6259.0
13	7883.5
14	11784.0
15	17235.5
16	21676.0
17	24070.5
18	25069.5
19	25155.0
20	24690.0
21	24502.5
22	25511.5
23	28534.5
24	34250.0
25	42279.5
26	52590.5
27	64161.5
28	75623.5
29	88393.5
30	100782.0
31	111622.5
32	123142.5
33	134873.0
34	145715.0
35	156665.0
36	169216.0
37	180402.5
38	189001.5
39	197765.5
40	207430.5
41	216891.5
42	223232.5
43	228317.0
44	234898.0
45	241197.5
46	245678.0
47	249431.5
48	252344.5
49	252042.5
50	250030.5
51	246019.0
52	238170.5
53	228267.0
54	216427.5
55	202724.0
56	187814.0
57	171259.0
58	153427.0
59	133431.0
60	113079.0
61	93715.0
62	75560.0
63	60013.0
64	46672.5
65	35912.0
66	27393.5
67	20960.0
68	16125.0
69	12135.0
70	8962.5
71	6441.0
72	4540.5
73	3159.5
74	2189.0
75	1523.0
76	1062.0
77	689.5
78	438.5
79	293.5
80	190.5
81	120.5
82	75.0
83	50.0
84	36.5
85	26.0
86	20.0
87	17.0
88	13.0
89	10.5
90	8.5
91	6.5
92	6.0
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0013873296065094209
18-19	1.3170850694709692E-4
20-21	0.0
22-23	0.0067794234662366705
24-25	0.008142225264016265
26-27	0.01984018023107923
28-29	0.01569386121210768
30-31	0.0017707322797270713
32-33	0.005834825634192502
34-35	0.03200999592947249
36-37	0.005803357738732834
38-39	0.0029790031731800164
40-41	0.007104622729028777
42-43	0.007519548060417357
44-45	0.008783702446205185
46-47	0.00545978832835167
48-49	0.001205478728610287
50-51	0.002817425320559585
52-53	0.0011059121279413102
54-55	0.005856500889109826
56-57	0.0012510666594338332
58-59	0.001357552474721917
60-61	8.511371397280054E-4
62-63	0.0017639993060219203
64-65	4.725163490656777E-4
66-67	0.005764447039036474
68-69	0.004117881360819652
70-71	0.001792246892937837
72-73	0.001594953920230595
74-75	8.528292723324005E-4
76-77	9.21305915875898E-4
78-79	8.534748594563341E-4
80-81	0.001065089242189004
82-83	0.007673643505329857
84-85	8.195227677887059E-4
86-87	7.342417943890466E-5
88-89	4.727533578551329E-4
90-91	3.9282956785072676E-4
92-93	0.0024811805043309524
94-95	0.003765009507639799
96-97	4.0798574987372844E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	403.0
22-23	777.0
24-25	2034.0
26-27	2338.0
28-29	8751.0
30-31	18722.0
32-33	24396.0
34-35	33398.0
36-37	50623.0
38-39	56611.0
40-41	55382.0
42-43	61857.0
44-45	68331.0
46-47	68317.0
48-49	63155.0
50-51	56948.0
52-53	53890.0
54-55	57411.0
56-57	60490.0
58-59	59837.0
60-61	57844.0
62-63	56560.0
64-65	59324.0
66-67	62801.0
68-69	63042.0
70-71	62028.0
72-73	58447.0
74-75	60036.0
76-77	59450.0
78-79	63290.0
80-81	63183.0
82-83	60788.0
84-85	61746.0
86-87	65599.0
88-89	71628.0
90-91	75817.0
92-93	81931.0
94-95	95226.0
96-97	209839.0
98-99	1161359.0
100-101	2340647.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.43890414650495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.76343454514868	81.15457591892485
2	6.070907878944138	10.738088799764046
3	1.2857088978947857	3.4112005794367644
4	0.419471867267071	1.4839052904555177
5	0.19388060336438614	0.8573294048404747
6	0.0882773117464785	0.4684289227114769
7	0.05188376710269346	0.3211980453888285
8	0.029264397618997226	0.20704890047453625
9	0.02082067666399369	0.16572220449770791
>10	0.07462430030614445	1.000258041148554
>50	0.0010889015420370773	0.06413368197000678
>100	6.084449507794603E-4	0.11497075729762557
>500	2.8407449761488677E-5	0.013139453089610441
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.756113425961292E-5	0.0	0.0	0.0
2	0.0	5.268340277883876E-5	0.0	0.0	0.0
3	0.0	5.268340277883876E-5	0.0	0.0	0.0
4	0.0	7.024453703845169E-5	0.0	0.0	0.0
5	0.0	7.024453703845169E-5	0.0	0.0	1.756113425961292E-5
6	1.2292793981729045E-4	7.024453703845169E-5	0.0	0.0	1.756113425961292E-5
7	1.2292793981729045E-4	7.024453703845169E-5	0.0	1.756113425961292E-5	1.756113425961292E-5
8	1.2292793981729045E-4	7.024453703845169E-5	0.0	1.756113425961292E-5	1.756113425961292E-5
9	1.2292793981729045E-4	2.1073361111535505E-4	0.0	1.756113425961292E-5	1.756113425961292E-5
10-11	1.2292793981729045E-4	2.1073361111535505E-4	0.0	1.756113425961292E-5	1.756113425961292E-5
12-13	1.2292793981729045E-4	2.1073361111535505E-4	0.0	1.756113425961292E-5	1.756113425961292E-5
14-15	1.2292793981729045E-4	2.634170138941938E-4	0.0	5.268340277883876E-5	7.902510416825815E-5
16-17	1.580502083365163E-4	2.634170138941938E-4	0.0	8.78056712980646E-5	9.658623842787106E-5
18-19	1.580502083365163E-4	2.809781481538067E-4	0.0	2.019530439855486E-4	1.4048907407690337E-4
20-21	1.580502083365163E-4	3.161004166730326E-4	0.0	9.834235185383236E-4	1.4048907407690337E-4
22-23	1.6683077546632275E-4	3.2488098380283905E-4	0.0	0.0031961264352495513	1.580502083365163E-4
24-25	1.756113425961292E-4	3.775643865816778E-4	0.0	0.006313227766330845	1.756113425961292E-4
26-27	1.756113425961292E-4	4.039060879710972E-4	0.0	0.011704495984032013	1.756113425961292E-4
28-29	1.756113425961292E-4	4.039060879710972E-4	0.0	0.03377006118123565	1.756113425961292E-4
30-31	1.756113425961292E-4	4.5658949074993594E-4	0.0	0.036931065347965975	1.756113425961292E-4
32-33	1.8439190972593565E-4	4.5658949074993594E-4	0.0	0.04285794816058534	1.756113425961292E-4
34-35	1.9317247685574212E-4	5.619562963076135E-4	0.0	0.05084826424870921	1.756113425961292E-4
36-37	1.9317247685574212E-4	6.497619676056781E-4	0.0	0.06430009309157271	1.756113425961292E-4
38-39	1.9317247685574212E-4	6.936648032547103E-4	0.0	0.09680575260611624	1.756113425961292E-4
40-41	1.9317247685574212E-4	7.902510416825815E-4	0.0	0.13083923080124607	1.756113425961292E-4
42-43	1.9317247685574212E-4	8.341538773316138E-4	0.0	0.15340528832484868	1.756113425961292E-4
44-45	1.9317247685574212E-4	9.395206828892912E-4	0.0	0.17906210547814316	1.756113425961292E-4
46-47	1.9317247685574212E-4	0.0010009846527979365	0.0	0.20681747817546137	1.756113425961292E-4
48-49	1.9317247685574212E-4	0.001027326354187356	0.0	0.23248307589588565	1.756113425961292E-4
50-51	2.195141782451615E-4	0.0011414737268748399	0.0	0.25790281773667534	1.756113425961292E-4
52-53	2.2829474537496797E-4	0.0011414737268748399	0.0	0.3153628490341288	1.9317247685574212E-4
54-55	2.2829474537496797E-4	0.0011678154282642591	0.0	0.3828941908294703	2.1073361111535505E-4
56-57	2.2829474537496797E-4	0.0011765959953940657	0.0	0.44962650101599944	2.1073361111535505E-4
58-59	2.2829474537496797E-4	0.0011765959953940657	0.0	0.4929849415029838	2.1073361111535505E-4
60-61	2.634170138941938E-4	0.001202937696783485	0.0	0.5243579078577822	2.1073361111535505E-4
62-63	3.161004166730326E-4	0.0012292793981729044	0.0	0.5480390974068703	2.2829474537496797E-4
64-65	3.161004166730326E-4	0.0014400130092882596	0.0	0.5566177114926911	2.2829474537496797E-4
66-67	3.4244211806245194E-4	0.0014751352778074854	0.0	0.5649943725345266	2.2829474537496797E-4
68-69	3.512226851922584E-4	0.0015014769791969048	0.0	0.5729671274883907	2.2829474537496797E-4
70-71	3.6000325232206484E-4	0.0015190381134565175	0.0	0.5798598726852888	2.634170138941938E-4
72-73	3.8634495371148424E-4	0.001527818680586324	0.0	0.5849174793520573	3.161004166730326E-4
74-75	3.8634495371148424E-4	0.0015541603819757433	0.0	0.5888862956947298	3.161004166730326E-4
76-77	3.8634495371148424E-4	0.001580502083365163	0.0	0.5933995071994504	3.161004166730326E-4
78-79	3.8634495371148424E-4	0.001580502083365163	0.0	0.5962005081138586	3.161004166730326E-4
80-81	3.9512552084129075E-4	0.001580502083365163	0.0	0.5969907591555412	3.161004166730326E-4
82-83	4.039060879710972E-4	0.001580502083365163	0.0	0.5973858846763825	3.336615509326455E-4
84-85	4.039060879710972E-4	0.001580502083365163	0.0	0.5977195462273153	3.336615509326455E-4
86-87	4.039060879710972E-4	0.0015980632176247759	0.0	0.5978073518986133	3.336615509326455E-4
88	4.039060879710972E-4	0.0016858688889228404	0.0	0.5978336936000027	3.336615509326455E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5400	0.0	42.357853	1
GTATCAA	9435	0.0	33.750015	1
TCAACGC	11265	0.0	27.362478	4
ATCAACG	11340	0.0	27.253418	3
CAACGCA	11595	0.0	26.583727	5
TATCAAC	12095	0.0	25.788162	2
AACGCAG	12075	0.0	25.62828	6
GTACATG	14795	0.0	22.404766	1
TACATGG	14785	0.0	22.17173	2
ACGCAGA	14255	0.0	21.680378	7
ACATGGG	14900	0.0	21.48069	3
CGCAGAG	14635	0.0	21.061722	8
CATGGGG	11820	0.0	19.523775	4
GCAGAGT	17680	0.0	17.388172	9
ACCGAAC	740	0.0	17.080273	8
AGAGTAC	15570	0.0	16.9694	10-11
GGACCGA	975	0.0	16.308907	6
GTGGTAT	3180	0.0	16.283262	1
GAGTACA	10470	0.0	16.277752	1
GAGTACT	10400	0.0	16.270273	12-13
>>END_MODULE