FastQCFastQC Report
Fri 27 May 2016
SRR2140229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7371626
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.043.728411
GTATCAA116000.032.9827651
ATCAACG131700.028.0555613
TCAACGC131750.028.0449094
CAACGCA134150.027.512645
AACGCAG138550.026.7276066
TATCAAC143300.026.1561052
GTACATG188650.022.6043531
TACATGG185850.022.3718362
ACGCAGA165750.022.3168227
CGCAGAG173700.021.2954148
ACATGGG191950.021.14873
CATGGGG146850.019.0242964
GCAGAGT209550.017.613089
GAGTACT117000.016.560512-13
AGAGTAC184200.016.32317410-11
TGGTATC36400.016.0928442
CAGAGTA203000.015.83051610-11
ATGGGGG91350.015.8293955
GTGGTAT41900.015.2513661
Produced by FastQC (version 0.11.5)