##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7371626
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.191211274147655	33.0	31.0	34.0	31.0	34.0
2	32.47441717743141	34.0	31.0	34.0	31.0	34.0
3	32.2660810518602	33.0	31.0	34.0	30.0	34.0
4	36.08430921481909	37.0	35.0	37.0	35.0	37.0
5	35.92733068660836	37.0	35.0	37.0	35.0	37.0
6	36.02587258224983	37.0	35.0	37.0	35.0	37.0
7	35.99274813996261	37.0	35.0	37.0	35.0	37.0
8	35.971064457149616	37.0	35.0	37.0	35.0	37.0
9	37.76609760180454	39.0	38.0	39.0	35.0	39.0
10-11	37.68780978579217	39.0	37.0	39.0	35.0	39.0
12-13	37.66164642644648	39.0	37.0	39.0	35.0	39.0
14-15	38.99517392770605	40.0	38.0	41.0	35.5	41.0
16-17	38.97504593966107	40.0	38.0	41.0	35.5	41.0
18-19	38.940117689096	40.0	38.0	41.0	35.0	41.0
20-21	38.93071664754681	40.0	38.0	41.0	35.0	41.0
22-23	38.83975003210729	40.0	38.0	41.0	35.0	41.0
24-25	38.77478935462566	40.0	38.0	41.0	34.5	41.0
26-27	38.62999670876365	40.0	38.0	41.0	34.0	41.0
28-29	38.50644800797885	40.0	38.0	41.0	34.0	41.0
30-31	38.363649399498854	40.0	38.0	41.0	34.0	41.0
32-33	38.206359422633696	40.0	38.0	41.0	34.0	41.0
34-35	38.31102256856054	40.0	38.0	41.0	34.0	41.0
36-37	38.305439696778976	40.0	38.0	41.0	34.0	41.0
38-39	38.240676149596965	40.0	38.0	41.0	33.5	41.0
40-41	38.219069407826595	40.0	38.0	41.0	33.0	41.0
42-43	38.286873929025404	40.0	38.0	41.0	33.0	41.0
44-45	38.24569264292832	40.0	38.0	41.0	33.0	41.0
46-47	38.15590352242735	40.0	38.0	41.0	33.0	41.0
48-49	38.05974192208239	40.0	37.0	41.0	33.0	41.0
50-51	37.948694446605785	40.0	37.0	41.0	33.0	41.0
52-53	37.79088188208863	40.0	37.0	41.0	33.0	41.0
54-55	37.62494258303465	40.0	36.0	41.0	32.0	41.0
56-57	37.513905446918656	39.0	36.0	41.0	32.0	41.0
58-59	37.30009802595062	39.0	36.0	41.0	32.0	41.0
60-61	37.09165596706489	39.0	35.0	41.0	31.0	41.0
62-63	36.83057783610493	38.5	35.0	40.0	31.0	41.0
64-65	36.51587360064342	38.0	35.0	40.0	31.0	41.0
66-67	36.223160731525624	37.0	35.0	40.0	31.0	41.0
68-69	35.93785625985031	37.0	34.5	39.5	30.0	41.0
70-71	35.62297834430706	36.5	34.0	39.0	30.0	41.0
72-73	35.15332029442483	36.0	34.0	39.0	29.0	40.0
74-75	34.91373745194926	35.5	34.0	38.5	29.0	40.0
76-77	33.687372511453034	34.5	32.5	36.5	28.5	39.0
78-79	34.40512160131319	35.0	34.0	37.0	29.5	39.0
80-81	34.31870365762366	35.0	34.0	36.5	30.0	39.0
82-83	34.07789899608319	35.0	34.0	36.0	30.0	37.5
84-85	33.85826561278787	35.0	34.0	36.0	29.0	37.0
86-87	33.669911206337595	35.0	33.5	35.0	29.0	37.0
88-89	33.49584009094978	35.0	33.0	35.0	29.0	36.0
90-91	33.34556187974181	35.0	33.0	35.0	29.0	36.0
92-93	33.26360910701368	35.0	33.0	35.0	29.0	36.0
94-95	33.223060915413875	35.0	33.0	35.0	29.0	35.5
96-97	33.27470766896947	35.0	33.0	35.0	29.0	35.0
98-99	33.37051734360347	35.0	33.0	35.0	30.0	35.0
100	32.149972150753804	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	30.0
21	216.0
22	883.0
23	3194.0
24	8908.0
25	18749.0
26	34155.0
27	55206.0
28	79407.0
29	107210.0
30	136927.0
31	172389.0
32	214373.0
33	274924.0
34	371294.0
35	525837.0
36	837463.0
37	1487601.0
38	1913436.0
39	1085331.0
40	44087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50390619383024	19.797450386115628	13.046226165027905	24.652417255026233
2	15.603830145479435	21.12523071571998	39.56410702333515	23.706832115465435
3	18.826402750220915	25.15076049707351	30.07762466516885	25.945212087536724
4	12.928857215490858	17.146461309892825	38.01249547928774	31.912185995328574
5	13.535114233955984	37.48546114520731	35.50306268928999	13.476361931546718
6	31.417356225071646	37.010192866539896	18.150161172039926	13.422289736348533
7	27.487626203499744	31.95848514289792	22.617411680950717	17.936476972651626
8	25.214423520672373	36.12006360604838	20.883153865917777	17.78235900736147
9	25.728977568856585	16.170353731998883	20.94858040817589	37.15208829096864
10-11	24.797025785084593	26.335397373659486	28.559608151580125	20.307968689675793
12-13	25.20651481776205	23.77080036344763	28.743590627088246	22.279094191702075
14-15	22.250002916588553	25.31612428519841	26.115283113929006	26.31858968428404
16-17	22.2401950397375	28.377341715382737	26.898400705624514	22.48406253925525
18-19	22.24823261516523	27.78717341330122	28.276454882545586	21.688139088987963
20-21	22.897167321232132	27.03142707888384	28.16165226581945	21.909753334064586
22-23	22.654973074721678	26.964320130871116	27.963912481499236	22.416794312907967
24-25	22.473132754786082	27.037053070349415	28.083163373939477	22.40665080092502
26-27	22.469371491623683	27.227501807626947	27.976186233333124	22.326940467416247
28-29	22.405560295437063	27.32617554609753	27.979429955987722	22.288834202477684
30-31	22.628237909526153	27.13355656889287	27.92626337221303	22.31194214936795
32-33	22.407059943266265	27.273205898554902	27.878216880037876	22.44151727814096
34-35	22.579441964657683	27.281000167122443	27.91451560242634	22.225042265793533
36-37	22.553374980520584	27.247006726316584	27.80926997980766	22.390348313355172
38-39	22.35422323666472	27.422746503015738	27.926048729683284	22.296981530636256
40-41	22.63177162318405	27.22675932357569	27.8146455840883	22.326823469151968
42-43	22.461211250752307	27.316766944390693	27.658656876199473	22.563364928657535
44-45	22.509674676135827	27.423257046907874	27.59096004924898	22.47610822770732
46-47	22.574368496411235	27.33435784666709	27.422378868791036	22.66889478813064
48-49	22.518562913637837	27.48983556276156	27.399148203936342	22.59245331966426
50-51	22.464961997132868	27.561113332108622	27.355702684549826	22.618221986208685
52-53	22.637370500846814	27.488982969566628	27.265350105096626	22.608296424489932
54-55	22.619598172651738	27.369719333324273	27.320161371357287	22.690521122666706
56-57	22.583455226941023	27.519791084738788	27.25714150086947	22.63961218745072
58-59	22.519231027822624	27.54238707641974	27.360684594821034	22.577697300936602
60-61	22.599563028199945	27.534476942899293	27.318971718733987	22.54698831016677
62-63	22.55767044021715	27.593771692691398	27.278198967453744	22.570358899637707
64-65	22.51673282316677	27.58891448905824	27.198961498953185	22.695391188821805
66-67	22.540952440878034	27.634638069678875	27.103118865977173	22.72129062346592
68-69	22.518962862487903	27.59730349268161	27.215139171107932	22.668594473722546
70-71	22.504340688246607	27.40333024063306	27.241871535044453	22.850457536075876
72-73	22.60446222562562	27.431409690860555	27.14402739797106	22.82010068554276
74-75	22.483460594748944	27.550845900648525	27.142773743683435	22.822919760919095
76-77	22.62078356220904	27.496517159198657	27.061364271663145	22.821335006929154
78-79	22.581970182596294	27.474936354762548	27.079569900813738	22.86352356182742
80-81	22.579676678081597	27.55157327386197	27.05645339383944	22.812296654216997
82-83	22.649868591214386	27.503124246145248	27.192177867096657	22.65482929554371
84-85	22.747790783404547	27.460197687897203	27.116983147407446	22.675028381290804
86-87	22.582860665994197	27.531179436374583	27.212287015121966	22.67367288250926
88-89	22.628901704736965	27.494935608388598	27.206370498492344	22.669792188382097
90-91	22.64796632047528	27.55349459786171	27.186919722783298	22.611619358879715
92-93	22.70801336885608	27.539163946631028	27.20504074971341	22.547781934799477
94-95	22.508758371198567	27.60956359003505	27.301178708863223	22.58049932990316
96-97	22.49188544578422	27.732583800047568	27.423435341877305	22.352095412290904
98-99	23.631639616889686	29.703682520676804	27.59410356178749	19.07057430064602
100	30.30211340704413	41.22496598448136	28.47292060847451	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1288.0
1	1153.5
2	1492.5
3	2141.5
4	2841.0
5	4496.0
6	6029.5
7	7004.5
8	7679.0
9	7643.0
10	7012.0
11	6357.5
12	6710.0
13	8559.0
14	12934.0
15	18752.5
16	23677.0
17	27119.5
18	28697.0
19	28650.5
20	27874.5
21	27374.5
22	28252.5
23	31305.0
24	37157.0
25	45849.0
26	57633.0
27	71531.5
28	85932.0
29	101715.5
30	118277.0
31	134305.0
32	150843.0
33	167262.0
34	182989.5
35	199883.0
36	218579.5
37	234869.0
38	247408.0
39	259068.5
40	271909.5
41	283746.5
42	292025.5
43	301134.0
44	310728.5
45	318733.5
46	325956.5
47	331686.0
48	334308.5
49	332818.0
50	328967.0
51	322609.0
52	310509.0
53	295655.5
54	279898.5
55	261318.5
56	242604.5
57	221076.0
58	196970.0
59	171951.0
60	146510.5
61	122244.5
62	99820.0
63	80294.5
64	63030.0
65	49452.5
66	38806.0
67	29710.0
68	22376.5
69	17019.5
70	12701.5
71	9201.0
72	6659.5
73	4668.0
74	3232.5
75	2268.0
76	1566.0
77	1047.0
78	694.5
79	447.5
80	300.0
81	200.0
82	144.5
83	96.5
84	56.5
85	34.0
86	27.0
87	24.5
88	17.5
89	10.0
90	5.0
91	2.0
92	2.5
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0052026072998753005
22-23	0.004931710395855572
24-25	0.0038063846165067496
26-27	0.016859969200012787
28-29	0.021572789485302855
30-31	0.03963868302670232
32-33	0.024392791530724472
34-35	0.012616598108483956
36-37	0.0073357308682517235
38-39	0.0030569757610372492
40-41	0.008170474632855996
42-43	0.010090075044311832
44-45	0.00612415551449332
46-47	0.00216595346470786
48-49	0.010659741635523644
50-51	0.0048807479270027605
52-53	0.0
54-55	5.012734243742076E-4
56-57	5.453593833844205E-4
58-59	3.575430666453281E-4
60-61	0.00106957145496243
62-63	0.0010102934584294073
64-65	0.0
66-67	1.95611855545535E-4
68-69	6.028834179558668E-4
70-71	2.8455216510720925E-4
72-73	7.800024993993134E-4
74-75	4.807693958686112E-4
76-77	8.697316764712969E-6
78-79	8.814518640459222E-6
80-81	5.275004302704781E-4
82-83	0.002267180923758333
84-85	3.6781183041005685E-5
86-87	7.650821721578575E-4
88-89	2.7487732177736433E-4
90-91	0.009131713834652529
92-93	9.427600301565365E-4
94-95	6.014328536304893E-5
96-97	2.3712391374050603E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	218.0
20-21	530.0
22-23	1163.0
24-25	2349.0
26-27	4211.0
28-29	10439.0
30-31	22184.0
32-33	29435.0
34-35	41085.0
36-37	62698.0
38-39	68780.0
40-41	69308.0
42-43	74313.0
44-45	84465.0
46-47	83923.0
48-49	78137.0
50-51	69808.0
52-53	67564.0
54-55	72584.0
56-57	76434.0
58-59	75702.0
60-61	73140.0
62-63	72096.0
64-65	76469.0
66-67	80380.0
68-69	80115.0
70-71	78177.0
72-73	73414.0
74-75	75275.0
76-77	74979.0
78-79	79913.0
80-81	79681.0
82-83	76080.0
84-85	77394.0
86-87	82673.0
88-89	87990.0
90-91	93284.0
92-93	98619.0
94-95	121205.0
96-97	274055.0
98-99	1527916.0
100-101	3093441.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.11503706813946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.1081761584185	79.36892143251208
2	6.361965019613159	11.084456370196138
3	1.4307687095896726	3.7392440751551517
4	0.5187556527847322	1.8076567168659532
5	0.21821653704886465	0.9504970856946438
6	0.11728328171462478	0.6130282460425548
7	0.07106149776606066	0.43333675084055195
8	0.04381950046106784	0.30538699255786234
9	0.027242122279329088	0.2135878642960687
>10	0.10100332482325522	1.3423840365114021
>50	0.0013812030227405147	0.08273664202924157
>100	3.032861457979265E-4	0.0438532020837396
>500	2.3706332190914468E-5	0.014910585214591442
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.0696584444191825E-5	0.0	0.0	0.0
3	0.0	6.78276407403197E-5	0.0	0.0	0.0
4	0.0	1.0852422518451153E-4	0.0	0.0	0.0
5	0.0	1.356552814806394E-4	0.0	1.3565528148063942E-5	0.0
6	2.7131056296127883E-5	1.356552814806394E-4	0.0	1.3565528148063942E-5	0.0
7	2.7131056296127883E-5	1.356552814806394E-4	0.0	1.3565528148063942E-5	0.0
8	2.7131056296127883E-5	1.627863377767673E-4	0.0	1.3565528148063942E-5	0.0
9	2.7131056296127883E-5	2.8487609110934277E-4	0.0	4.0696584444191825E-5	0.0
10-11	2.7131056296127883E-5	2.916588551833747E-4	0.0	6.104487666628773E-5	0.0
12-13	2.7131056296127883E-5	3.1200714740547064E-4	0.0	8.139316888838365E-5	0.0
14-15	3.391382037015985E-5	4.2053137258998217E-4	0.0	8.139316888838365E-5	6.782764074031971E-6
16-17	4.74793485182238E-5	4.273141366640141E-4	0.0	8.139316888838365E-5	2.7131056296127883E-5
18-19	5.426211259225577E-5	4.6801072110820596E-4	0.0	1.0852422518451153E-4	2.7131056296127883E-5
20-21	5.426211259225577E-5	5.154900696264298E-4	0.0	1.9670015814692715E-4	2.7131056296127883E-5
22-23	5.426211259225577E-5	5.968832385148135E-4	0.0	3.5270373184966247E-4	4.747934851822379E-5
24-25	6.104487666628773E-5	6.918419355512609E-4	0.0	4.5444519296014205E-4	6.78276407403197E-5
26-27	6.78276407403197E-5	7.189729918473888E-4	0.0	6.036660025888454E-4	6.78276407403197E-5
28-29	6.78276407403197E-5	7.393212840694848E-4	0.0	0.001390466635176554	6.78276407403197E-5
30-31	6.78276407403197E-5	8.410627451799643E-4	0.0	0.004184965433677726	6.78276407403197E-5
32-33	8.817593296241562E-5	8.546282733280283E-4	0.0	0.00984179067142039	6.78276407403197E-5
34-35	1.2887251740660745E-4	9.699352625865718E-4	0.0	0.017499531311002486	6.78276407403197E-5
36-37	1.627863377767673E-4	0.0010784594877710834	0.0	0.029925555094629054	6.78276407403197E-5
38-39	1.627863377767673E-4	0.0010988077799931792	0.0	0.06098383178962145	6.78276407403197E-5
40-41	1.627863377767673E-4	0.0011666354207334989	0.0	0.09317483008497718	6.78276407403197E-5
42-43	1.627863377767673E-4	0.001180200948881563	0.0	0.11327216003633392	6.78276407403197E-5
44-45	1.627863377767673E-4	0.0012751596459180106	0.0	0.1368626134858171	7.461040481435168E-5
46-47	1.627863377767673E-4	0.0013429872866583302	0.0	0.1624878961575099	8.139316888838365E-5
48-49	1.627863377767673E-4	0.001356552814806394	0.0	0.1871025469821719	8.139316888838365E-5
50-51	1.627863377767673E-4	0.0014379459836947777	0.0	0.21046916921721204	8.139316888838365E-5
52-53	1.7635186592483124E-4	0.0014379459836947777	0.0	0.2667593282676034	8.817593296241562E-5
54-55	1.8991739407289517E-4	0.0014447287477688097	0.0	0.33096497299239	9.495869703644758E-5
56-57	1.8991739407289517E-4	0.0014922080962870335	0.0	0.39495356926680764	9.495869703644758E-5
58-59	1.8991739407289517E-4	0.0015057736244350976	0.0	0.4432129356535451	9.495869703644758E-5
60-61	1.8991739407289517E-4	0.0015193391525831616	0.0	0.4786460951762881	9.495869703644758E-5
62-63	1.8991739407289517E-4	0.0015329046807312254	0.0	0.5028469973924341	9.495869703644758E-5
64-65	1.8991739407289517E-4	0.0016685599622118649	0.0	0.5115831975197873	9.495869703644758E-5
66-67	1.8991739407289517E-4	0.0017092565466560567	0.0	0.5201633940734378	9.495869703644758E-5
68-69	1.8991739407289517E-4	0.0017296048388781525	0.0	0.5281399246244993	9.495869703644758E-5
70-71	1.9670015814692715E-4	0.0017703014233223444	0.0	0.5350854750363081	9.495869703644758E-5
72-73	2.0348292222095913E-4	0.0017906497155444402	0.0	0.5403760310140531	9.495869703644758E-5
74-75	2.0348292222095913E-4	0.001838129064062664	0.0	0.5445135170992126	9.495869703644758E-5
76-77	2.0348292222095913E-4	0.0019059567048029837	0.0	0.5483389960369667	9.495869703644758E-5
78-79	2.1704845036902307E-4	0.0019466532892471757	0.0	0.5510114050821353	9.495869703644758E-5
80-81	2.1704845036902307E-4	0.0019737843455433033	0.0	0.5518795988836114	9.495869703644758E-5
82-83	2.1704845036902307E-4	0.0019941326377653995	0.0	0.5524968304143483	9.495869703644758E-5
84-85	2.1704845036902307E-4	0.002014480929987495	0.0	0.5527206616287913	9.495869703644758E-5
86-87	2.1704845036902307E-4	0.002061960278505719	0.0	0.5527952720336056	9.495869703644758E-5
88	2.1704845036902307E-4	0.0021704845036902305	0.0	0.5527952720336056	9.495869703644758E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6445	0.0	43.72841	1
GTATCAA	11600	0.0	32.982765	1
ATCAACG	13170	0.0	28.055561	3
TCAACGC	13175	0.0	28.044909	4
CAACGCA	13415	0.0	27.51264	5
AACGCAG	13855	0.0	26.727606	6
TATCAAC	14330	0.0	26.156105	2
GTACATG	18865	0.0	22.604353	1
TACATGG	18585	0.0	22.371836	2
ACGCAGA	16575	0.0	22.316822	7
CGCAGAG	17370	0.0	21.295414	8
ACATGGG	19195	0.0	21.1487	3
CATGGGG	14685	0.0	19.024296	4
GCAGAGT	20955	0.0	17.61308	9
GAGTACT	11700	0.0	16.5605	12-13
AGAGTAC	18420	0.0	16.323174	10-11
TGGTATC	3640	0.0	16.092844	2
CAGAGTA	20300	0.0	15.830516	10-11
ATGGGGG	9135	0.0	15.829395	5
GTGGTAT	4190	0.0	15.251366	1
>>END_MODULE