FastQCFastQC Report
Fri 27 May 2016
SRR2140228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20368276
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224550.046.2927741
GTATCAA388250.034.8637961
ATCAACG452700.028.9702823
TCAACGC456650.028.7289124
CAACGCA458300.028.5857835
AACGCAG479550.027.4487386
TATCAAC490700.027.1043342
ACGCAGA563550.023.267737
CGCAGAG574850.022.8249958
GTACATG554100.022.2402531
TACATGG544100.021.999022
ACATGGG557800.020.9756413
CATGGGG409500.018.9382954
GCAGAGT693050.018.8896649
GTGGTAT118650.018.735761
GAGTACT415400.018.30120312-13
CAGAGTA667250.017.4183210-11
AGAGTAC612350.017.161710-11
TGGTATC120050.016.6935982
GAGTACA395000.016.5424481
Produced by FastQC (version 0.11.5)