FastQCFastQC Report
Fri 27 May 2016
SRR2140227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7281794
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT106600.064.0962794
TATCTCG135100.050.13120794
GGTATCA67650.045.234911
GCCGTCT63850.036.8778490-91
CCGTCTT61950.036.8070190-91
TGCCGTC62350.035.3756788-89
TATGCCG61200.035.3045586-87
ATGCCGT65200.035.1251388-89
GTATCAA112750.033.1031761
TCGTATG70650.031.94847384-85
TCTCGTA68850.031.73117482-83
CGTATGC68250.031.3715884-85
CTCGTAT67300.031.36157882-83
CTATCTC160150.029.68867992-93
GTATGCC77400.029.21360686-87
TCAACGC136200.026.7980864
ATCAACG137750.026.4666063
CAACGCA140000.025.9528735
ACGCTAT279650.025.46880394
TATCAAC144550.025.3927362
Produced by FastQC (version 0.11.5)