##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7281794
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21726321837723	33.0	31.0	34.0	31.0	34.0
2	32.50056126278771	34.0	31.0	34.0	31.0	34.0
3	32.30109036317149	34.0	31.0	34.0	30.0	34.0
4	36.10194616876006	37.0	35.0	37.0	35.0	37.0
5	35.94612234292813	37.0	35.0	37.0	35.0	37.0
6	36.043207621638295	37.0	35.0	37.0	35.0	37.0
7	36.0092791693915	37.0	35.0	37.0	35.0	37.0
8	35.98945026459139	37.0	35.0	37.0	35.0	37.0
9	37.785417714370936	39.0	38.0	39.0	35.0	39.0
10-11	37.71232151033111	39.0	37.5	39.0	35.0	39.0
12-13	37.68508090176679	39.0	37.0	39.0	35.0	39.0
14-15	39.02877834775332	40.0	38.0	41.0	36.0	41.0
16-17	39.01149867738637	40.0	38.0	41.0	36.0	41.0
18-19	38.98122763428903	40.0	38.5	41.0	35.0	41.0
20-21	38.97383126880078	40.0	38.5	41.0	35.0	41.0
22-23	38.88514674952445	40.0	38.0	41.0	35.0	41.0
24-25	38.824481261827536	40.0	38.0	41.0	35.0	41.0
26-27	38.68241670281553	40.0	38.0	41.0	34.5	41.0
28-29	38.564502160413824	40.0	38.0	41.0	34.0	41.0
30-31	38.428297492746324	40.0	38.0	41.0	34.0	41.0
32-33	38.276722162142484	40.0	38.0	41.0	34.0	41.0
34-35	38.366320367700766	40.0	38.0	41.0	34.0	41.0
36-37	38.36065053094777	40.0	38.0	41.0	34.0	41.0
38-39	38.2984342856313	40.0	38.0	41.0	34.0	41.0
40-41	38.27410240036235	40.0	38.0	41.0	33.5	41.0
42-43	38.34103141064011	40.0	38.0	41.0	34.0	41.0
44-45	38.30194903725635	40.0	38.0	41.0	33.5	41.0
46-47	38.21894453055841	40.0	38.0	41.0	33.0	41.0
48-49	38.11999216249015	40.0	37.0	41.0	33.0	41.0
50-51	38.00935366696413	40.0	37.0	41.0	33.0	41.0
52-53	37.85814333198401	40.0	37.0	41.0	33.0	41.0
54-55	37.70121836418216	40.0	36.0	41.0	33.0	41.0
56-57	37.58709371338253	39.0	36.0	41.0	32.5	41.0
58-59	37.38421700788919	39.0	36.0	41.0	32.0	41.0
60-61	37.176973967623525	39.0	35.0	41.0	32.0	41.0
62-63	36.92348788386892	38.5	35.0	40.0	31.0	41.0
64-65	36.619873908707255	38.0	35.0	40.0	31.0	41.0
66-67	36.332943880998556	37.0	35.0	40.0	31.0	41.0
68-69	36.03716271038725	37.0	35.0	39.5	31.0	41.0
70-71	35.724679596002524	36.5	34.0	39.0	30.5	41.0
72-73	35.25905923158177	36.0	34.0	39.0	30.0	40.5
74-75	34.99215432876042	35.0	34.0	38.5	30.0	40.0
76-77	33.7309213905546	34.5	32.5	36.5	28.5	39.0
78-79	34.39131429019373	35.0	34.0	37.0	29.5	39.0
80-81	34.27982403926332	35.0	34.0	36.5	30.0	39.0
82-83	34.03923082257508	35.0	34.0	36.0	30.0	37.5
84-85	33.855866927224255	35.0	34.0	35.5	30.0	37.0
86-87	33.682317083395716	35.0	33.5	35.0	29.5	37.0
88-89	33.52706536540789	35.0	33.0	35.0	29.0	36.0
90-91	33.408208205536454	35.0	33.0	35.0	29.0	36.0
92-93	33.34439118235443	35.0	33.0	35.0	29.0	36.0
94-95	33.28084592137796	35.0	33.0	35.0	29.0	35.5
96-97	33.30893653759402	35.0	33.0	35.0	29.0	35.0
98-99	33.40306044155869	35.0	33.0	35.0	30.0	35.0
100	32.23908627413108	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	32.0
21	208.0
22	955.0
23	3233.0
24	8547.0
25	18393.0
26	33368.0
27	53663.0
28	76475.0
29	102714.0
30	131574.0
31	163800.0
32	205113.0
33	262942.0
34	357866.0
35	510491.0
36	822880.0
37	1492499.0
38	1949428.0
39	1039121.0
40	48488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55469187950112	19.71324648843403	13.049682537023157	24.68237909504169
2	15.673101985582125	21.046846422735936	39.35243155738819	23.92762003429375
3	18.671895964098955	25.257443426715998	30.112799126149408	25.95786148303564
4	13.083190763155345	17.099879507714718	37.698251282582284	32.11867844654765
5	13.610602002748223	37.49048654768317	35.35035459668318	13.54855685288543
6	31.539604114041126	36.788310682779546	18.070025051518897	13.602060151660428
7	27.636225358750877	31.8749747658338	22.63175805302924	17.857041822386076
8	25.442370382902897	35.807645753230595	20.863594877855647	17.886388986010864
9	25.94547442567038	16.033617539853502	20.866355186647688	37.154552847828434
10-11	24.966553571825845	26.170212999708585	28.4514296888926	20.411803739572967
12-13	25.364031171439343	23.667725288575863	28.60475728920648	22.363486250778312
14-15	22.449866063225627	25.159212139206353	25.948138604305477	26.442783193262535
16-17	22.399898980937937	28.194926964426624	26.76338413308589	22.641789921549552
18-19	22.44617191862335	27.657655517307962	28.048946454678614	21.847226109390075
20-21	23.123577442901148	26.88503043315093	27.921024539828853	22.07036758411907
22-23	22.93724112241136	26.73964091319404	27.748587041382507	22.57453092301209
24-25	22.67154492230217	26.836936225025305	27.91193751641716	22.57958133625536
26-27	22.59360785199099	27.037101564618204	27.81924228506903	22.550048298321776
28-29	22.601679069376132	27.077688585400455	27.802197507378374	22.51843483784504
30-31	22.80957363518424	26.97461466333526	27.693281351516212	22.52253034996429
32-33	22.573831543072917	27.121735816343385	27.683850499422917	22.62058214116078
34-35	22.78447419892859	27.096187017671546	27.69636695475619	22.42297182864367
36-37	22.72572606296361	27.005675426200355	27.66780965148487	22.600788859351166
38-39	22.488735348424687	27.13078334171103	27.649229816139993	22.73125149372429
40-41	22.93448763437866	26.869241774289282	27.73634394489376	22.459926646438298
42-43	22.52673139639427	26.9944963021974	27.555476054893447	22.923296246514884
44-45	22.54596902724031	27.05292774532275	27.52973907911479	22.87136414832215
46-47	22.599887294845914	26.983868880361634	27.639994521050877	22.776249303741576
48-49	22.52721583894154	27.370178822155903	27.349005815028583	22.753599523873973
50-51	22.50865557465872	27.445990575924277	27.05250798837134	22.992845861045666
52-53	22.495706729400894	27.151558372057178	27.005539048558376	23.347195849983553
54-55	22.728208310090654	26.752192078191168	27.550539394611096	22.96906021710709
56-57	22.430018703444823	26.314493128680176	27.756552390132676	23.49893577774233
58-59	22.129926470244257	26.004765679743592	27.80237529356033	24.062932556451816
60-61	22.418989546700917	26.504960155681996	28.228679619765945	22.847370677851146
62-63	21.79713278474493	26.788278040918044	28.226727585358013	23.18786158897901
64-65	21.481863306180614	27.285087003121237	27.67526190927813	23.55778778142001
66-67	21.52436158255669	28.294645287725672	26.9100690059231	23.270924123794543
68-69	21.804252845608108	28.326797317204853	26.58210053497907	23.286849302207973
70-71	21.77351127082257	27.56127245289018	26.620142112481805	24.045074163805445
72-73	22.132205759980344	26.646341514718834	26.599888431827722	24.621564293473096
74-75	22.24505813946049	26.715148419386864	26.935489002974638	24.104304438178005
76-77	22.78129186306047	26.638444966863428	25.908494655501542	24.671768514574563
78-79	23.11385252999135	26.64381025892291	25.227994159711404	25.014343051374333
80-81	22.766908799446288	27.268897183741082	25.546715419501435	24.417478597311195
82-83	22.73486804568006	26.945989527067145	25.61009142094181	24.709051006310983
84-85	22.818775609707256	27.610283003885183	25.52402045754556	24.046920928861997
86-87	23.73413795374403	27.357868211131887	25.621193710572843	23.28680012455124
88-89	23.142077780257388	27.644409533923366	26.217606378584684	22.995906307234566
90-91	23.14477111481797	26.944774494444136	25.915369332789375	23.99508505794852
92-93	23.110503752204387	26.22704392875996	26.336734495115476	24.32571782392018
94-95	21.895021627739347	26.906014519193693	27.488726649458478	23.710237203608475
96-97	21.95694762713273	26.62343086082548	27.916261642874584	23.503359869167205
98-99	23.336271176795613	28.476089885619142	28.23052257970956	19.957116357875684
100	30.39002687429026	40.289789434774065	29.320183690935668	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1470.0
1	1337.0
2	1766.5
3	2484.5
4	3152.0
5	4843.5
6	6413.0
7	7527.0
8	8261.0
9	8137.0
10	7545.0
11	6941.0
12	7219.5
13	8891.5
14	13269.0
15	19292.0
16	24365.5
17	27621.0
18	28802.5
19	28402.0
20	27231.0
21	26270.0
22	26159.0
23	28219.0
24	33151.5
25	40626.0
26	51229.0
27	63447.0
28	76518.0
29	91745.0
30	106669.0
31	120666.0
32	136742.0
33	153608.0
34	168599.5
35	183780.0
36	201098.0
37	216679.0
38	230556.5
39	243960.5
40	257390.5
41	270130.5
42	279087.5
43	288291.5
44	298585.5
45	307605.0
46	315709.0
47	323714.5
48	329825.0
49	332657.0
50	332509.5
51	328845.5
52	319949.0
53	306855.0
54	291696.5
55	274244.5
56	254044.5
57	231519.0
58	207869.5
59	181595.0
60	154170.0
61	127959.5
62	104200.5
63	83267.5
64	64873.0
65	50137.0
66	39028.0
67	30591.5
68	23668.5
69	17975.5
70	13577.0
71	10056.5
72	7354.5
73	5280.5
74	3702.0
75	2595.0
76	1802.0
77	1201.0
78	779.0
79	513.0
80	336.5
81	227.0
82	172.5
83	125.5
84	88.0
85	58.5
86	38.5
87	31.0
88	27.5
89	20.5
90	12.0
91	5.5
92	1.5
93	0.5
94	1.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005129440433669125
22-23	0.004717880692282721
24-25	0.0037916025345489175
26-27	0.016965965819041792
28-29	0.021747047178093667
30-31	0.039206814706856684
32-33	0.024255419183123156
34-35	0.012481316290469311
36-37	0.007235303062797733
38-39	0.0027991636155665447
40-41	0.007694336184159764
42-43	0.009649743867093746
44-45	0.006288526812897922
46-47	0.001985301803283704
48-49	0.010063488596940909
50-51	0.004998069239006302
52-53	0.0
54-55	4.5127499719291655E-4
56-57	6.356725982834083E-4
58-59	3.4510451605303504E-4
60-61	8.831404536506587E-4
62-63	9.259982241237447E-4
64-65	0.0
66-67	2.498952455253032E-4
68-69	4.988468052092364E-4
70-71	3.069804704002793E-4
72-73	7.5724430384007E-4
74-75	3.156243074392308E-4
76-77	0.0
78-79	1.7558437773116013E-5
80-81	4.4573414151470624E-4
82-83	0.002570378595045541
84-85	4.592740567291641E-5
86-87	8.302103277231949E-4
88-89	1.8968491533556068E-4
90-91	0.009481366363375586
92-93	0.0010132747850701536
94-95	5.021646308577755E-5
96-97	2.0649072624337098E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	202.0
20-21	566.0
22-23	1206.0
24-25	2584.0
26-27	4875.0
28-29	12323.0
30-31	26781.0
32-33	35682.0
34-35	48961.0
36-37	72666.0
38-39	43844.0
40-41	79497.0
42-43	83425.0
44-45	95586.0
46-47	93606.0
48-49	87165.0
50-51	45011.0
52-53	8650.0
54-55	8568.0
56-57	8563.0
58-59	46418.0
60-61	84042.0
62-63	81098.0
64-65	85659.0
66-67	88822.0
68-69	87955.0
70-71	85145.0
72-73	81277.0
74-75	82468.0
76-77	82425.0
78-79	86467.0
80-81	85356.0
82-83	81205.0
84-85	82066.0
86-87	87197.0
88-89	91894.0
90-91	95680.0
92-93	99997.0
94-95	119798.0
96-97	296532.0
98-99	1587662.0
100-101	3002870.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.66899081921711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.22984940019084	79.98028829503892
2	6.431305307401349	11.276520919003023
3	1.37000434469787	3.603206949528154
4	0.454351337584823	1.5933009297369145
5	0.1998284928580236	0.8759381152894031
6	0.10682236662220436	0.5619005447213448
7	0.057577190525078134	0.3533413931277573
8	0.04176421426030516	0.2929141213246808
9	0.025219362860453242	0.19898604819716223
>10	0.08161536911780481	1.101388844106516
>50	0.0013083987807567404	0.075442298287657
>100	3.090692599467482E-4	0.05534589409401265
>500	4.5145840467709496E-5	0.03142564754443801
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.373287956237158E-5	0.0	1.373287956237158E-5	0.0
3	0.0	1.373287956237158E-5	0.0	1.373287956237158E-5	0.0
4	0.0	4.119863868711474E-5	0.0	2.746575912474316E-5	0.0
5	0.0	4.119863868711474E-5	0.0	2.746575912474316E-5	0.0
6	6.86643978118579E-5	4.119863868711474E-5	0.0	2.746575912474316E-5	0.0
7	6.86643978118579E-5	4.119863868711474E-5	0.0	6.86643978118579E-5	0.0
8	6.86643978118579E-5	4.119863868711474E-5	0.0	6.86643978118579E-5	1.373287956237158E-5
9	6.86643978118579E-5	1.0986303649897264E-4	0.0	8.239727737422948E-5	2.746575912474316E-5
10-11	6.86643978118579E-5	1.0986303649897264E-4	0.0	8.926371715541527E-5	2.746575912474316E-5
12-13	6.86643978118579E-5	1.235959160613442E-4	0.0	9.613015693660106E-5	6.86643978118579E-5
14-15	6.86643978118579E-5	1.5106167518608737E-4	0.0	1.0299659671778685E-4	1.1672947628015842E-4
16-17	1.0986303649897264E-4	1.6479455474845896E-4	0.0	1.235959160613442E-4	1.5106167518608737E-4
18-19	1.0986303649897264E-4	1.7852743431083054E-4	0.0	1.5792811496727317E-4	1.991267536543879E-4
20-21	1.0986303649897264E-4	1.9226031387320212E-4	0.0	1.7852743431083054E-4	2.1972607299794528E-4
22-23	1.0986303649897264E-4	2.2659251277913108E-4	0.0	2.815240310286174E-4	2.8839047080980317E-4
24-25	1.1672947628015842E-4	2.471918321226884E-4	0.0	4.188528266523332E-4	3.8452062774640424E-4
26-27	1.235959160613442E-4	2.471918321226884E-4	0.0	6.042467007443495E-4	3.982535073087758E-4
28-29	1.235959160613442E-4	2.471918321226884E-4	0.0	0.001977534656981508	4.051199470899616E-4
30-31	1.235959160613442E-4	2.746575912474316E-4	0.0	0.006454453394314642	4.119863868711474E-4
32-33	1.373287956237158E-4	2.746575912474316E-4	0.0	0.014948239403641466	4.119863868711474E-4
34-35	1.6479455474845896E-4	3.227226697157321E-4	0.0	0.025550022425792324	4.119863868711474E-4
36-37	1.6479455474845896E-4	3.7078774818403266E-4	0.0	0.04781788663617784	4.25719266433519E-4
38-39	1.6479455474845896E-4	3.776541879652184E-4	0.0	0.33567277514304855	4.3945214599589057E-4
40-41	1.6479455474845896E-4	4.6691790512063373E-4	0.0	0.6347199604932521	4.3945214599589057E-4
42-43	1.6479455474845896E-4	4.7378434490181955E-4	0.0	0.683540347337483	4.669179051206337E-4
44-45	1.7166099452964475E-4	5.355823029324916E-4	0.0	0.7479200867258811	4.943836642453768E-4
46-47	1.7852743431083054E-4	5.973802609631637E-4	0.0	0.8169758716052664	4.943836642453768E-4
48-49	1.7852743431083054E-4	6.042467007443495E-4	0.0	0.88196672413419	5.081165438077485E-4
50-51	1.7852743431083054E-4	6.317124598690927E-4	0.0	0.9422197332140954	5.081165438077485E-4
52-53	1.8539387409201633E-4	6.317124598690927E-4	0.0	1.7166511439351346	5.2184942337012E-4
54-55	1.9226031387320212E-4	6.454453394314642E-4	0.0	2.75331875633944	5.355823029324916E-4
56-57	1.9226031387320212E-4	6.729110985562075E-4	0.0	3.869911727796749	5.355823029324916E-4
58-59	1.9226031387320212E-4	6.729110985562075E-4	0.0	4.980977215230203	5.424487427136774E-4
60-61	1.9226031387320212E-4	6.935104178997648E-4	0.0	5.280285325292091	5.630480620572348E-4
62-63	2.3345895256031687E-4	7.141097372433223E-4	0.0	5.291855276323389	5.90513821181978E-4
64-65	2.3345895256031687E-4	7.55308375930437E-4	0.0	5.301557555734204	5.90513821181978E-4
66-67	2.6092471168506E-4	7.690412554928085E-4	0.0	5.3106693213238385	5.973802609631637E-4
68-69	2.6092471168506E-4	7.759076952739944E-4	0.0	5.319369100526601	6.042467007443495E-4
70-71	2.815240310286174E-4	7.827741350551801E-4	0.0	5.326743656851594	6.042467007443495E-4
72-73	3.0212335037217475E-4	7.827741350551801E-4	0.0	5.332360404592604	6.454453394314643E-4
74-75	3.0212335037217475E-4	7.896405748363659E-4	0.0	5.336699994534314	6.454453394314643E-4
76-77	3.0212335037217475E-4	8.033734543987374E-4	0.0	5.341149447512523	6.454453394314643E-4
78-79	3.0212335037217475E-4	8.102398941799233E-4	0.0	5.344102016618432	6.454453394314643E-4
80-81	3.1585622993454633E-4	8.102398941799233E-4	0.0	5.344974054470643	6.591782189938359E-4
82-83	3.1585622993454633E-4	8.102398941799233E-4	0.0	5.345605766930512	6.729110985562075E-4
84-85	3.1585622993454633E-4	8.239727737422948E-4	0.0	5.345859825202416	6.729110985562075E-4
86-87	3.3645554927810373E-4	8.377056533046664E-4	0.0	5.345935356040009	6.797775383373932E-4
88	3.433219890592895E-4	8.789042919917811E-4	0.0	5.345935356040009	6.86643978118579E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	10660	0.0	64.09627	94
TATCTCG	13510	0.0	50.131207	94
GGTATCA	6765	0.0	45.23491	1
GCCGTCT	6385	0.0	36.87784	90-91
CCGTCTT	6195	0.0	36.80701	90-91
TGCCGTC	6235	0.0	35.37567	88-89
TATGCCG	6120	0.0	35.30455	86-87
ATGCCGT	6520	0.0	35.12513	88-89
GTATCAA	11275	0.0	33.103176	1
TCGTATG	7065	0.0	31.948473	84-85
TCTCGTA	6885	0.0	31.731174	82-83
CGTATGC	6825	0.0	31.37158	84-85
CTCGTAT	6730	0.0	31.361578	82-83
CTATCTC	16015	0.0	29.688679	92-93
GTATGCC	7740	0.0	29.213606	86-87
TCAACGC	13620	0.0	26.798086	4
ATCAACG	13775	0.0	26.466606	3
CAACGCA	14000	0.0	25.952873	5
ACGCTAT	27965	0.0	25.468803	94
TATCAAC	14455	0.0	25.392736	2
>>END_MODULE