FastQCFastQC Report
Fri 27 May 2016
SRR2140226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7723746
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT104350.059.57084794
TATCTCG129400.052.6975494
GGTATCA68500.045.8249241
CCGTCTT56550.034.9266890-91
GTATCAA118550.034.2041171
ATGCCGT59750.032.91964388-89
TATGCCG57150.032.8984186-87
TGCCGTC59600.032.5524688-89
GCCGTCT62400.032.24118490-91
CGTATGC61700.030.2385584-85
TCGTATG65100.029.5397484-85
CTCGTAT63200.029.18090682-83
CTATCTC158250.028.85601692-93
TCTCGTA65100.028.50224182-83
TCAACGC141200.027.8918574
ATCAACG142650.027.5499763
CGCTATC165650.027.0050892-93
CAACGCA147250.026.7176045
TATCAAC151750.026.3916992
GTATGCC74200.026.28317586-87
Produced by FastQC (version 0.11.5)