##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7723746
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.219338906276825	33.0	31.0	34.0	31.0	34.0
2	32.49999637481606	34.0	31.0	34.0	31.0	34.0
3	32.301523250505646	34.0	31.0	34.0	30.0	34.0
4	36.09971638114459	37.0	35.0	37.0	35.0	37.0
5	35.94562457128963	37.0	35.0	37.0	35.0	37.0
6	36.0435765236195	37.0	35.0	37.0	35.0	37.0
7	36.009049106482784	37.0	35.0	37.0	35.0	37.0
8	35.988399152431995	37.0	35.0	37.0	35.0	37.0
9	37.78588394802211	39.0	38.0	39.0	35.0	39.0
10-11	37.71126769316339	39.0	37.5	39.0	35.0	39.0
12-13	37.68413960013703	39.0	37.0	39.0	35.0	39.0
14-15	39.02845685241333	40.0	38.0	41.0	36.0	41.0
16-17	39.01086953144238	40.0	38.0	41.0	36.0	41.0
18-19	38.97475752050883	40.0	38.5	41.0	35.0	41.0
20-21	38.96764472050993	40.0	38.0	41.0	35.0	41.0
22-23	38.8780758036061	40.0	38.0	41.0	35.0	41.0
24-25	38.816317491128714	40.0	38.0	41.0	35.0	41.0
26-27	38.67507968154584	40.0	38.0	41.0	34.0	41.0
28-29	38.554477606353245	40.0	38.0	41.0	34.0	41.0
30-31	38.41845532224496	40.0	38.0	41.0	34.0	41.0
32-33	38.26659644813412	40.0	38.0	41.0	34.0	41.0
34-35	38.360495624591124	40.0	38.0	41.0	34.0	41.0
36-37	38.35306572648861	40.0	38.0	41.0	34.0	41.0
38-39	38.28646723827666	40.0	38.0	41.0	34.0	41.0
40-41	38.265350286922676	40.0	38.0	41.0	33.5	41.0
42-43	38.32979642407197	40.0	38.0	41.0	34.0	41.0
44-45	38.29094169831255	40.0	38.0	41.0	33.5	41.0
46-47	38.20278495635421	40.0	38.0	41.0	33.0	41.0
48-49	38.100366226575126	40.0	37.0	41.0	33.0	41.0
50-51	37.986880805442425	40.0	37.0	41.0	33.0	41.0
52-53	37.83048027295962	40.0	37.0	41.0	33.0	41.0
54-55	37.6683562930597	40.0	36.0	41.0	33.0	41.0
56-57	37.554119993503505	39.0	36.0	41.0	32.0	41.0
58-59	37.348208416226726	39.0	35.5	41.0	32.0	41.0
60-61	37.136753710790565	39.0	35.0	41.0	32.0	41.0
62-63	36.8781900154296	38.5	35.0	40.0	31.0	41.0
64-65	36.57238323228229	38.0	35.0	40.0	31.0	41.0
66-67	36.28949329404924	37.0	35.0	40.0	31.0	41.0
68-69	35.997404380526355	37.0	35.0	39.5	31.0	41.0
70-71	35.68331040380047	36.0	34.0	39.0	30.5	41.0
72-73	35.222241289726874	36.0	34.0	39.0	30.0	40.5
74-75	34.95545276165757	35.0	34.0	38.0	30.0	40.0
76-77	33.69845309930977	34.5	32.5	36.5	28.5	39.0
78-79	34.37023161422691	35.0	34.0	37.0	29.5	39.0
80-81	34.26468826237824	35.0	34.0	36.0	30.0	39.0
82-83	34.027131891084665	35.0	34.0	36.0	30.0	37.5
84-85	33.8439010668677	35.0	34.0	35.5	30.0	37.0
86-87	33.66927092745689	35.0	33.0	35.0	29.5	37.0
88-89	33.51338752546832	35.0	33.0	35.0	29.0	36.0
90-91	33.39380973206782	35.0	33.0	35.0	29.0	36.0
92-93	33.3277140418556	35.0	33.0	35.0	29.0	36.0
94-95	33.26394417278832	35.0	33.0	35.0	29.0	35.5
96-97	33.30081624603207	35.0	33.0	35.0	29.0	35.0
98-99	33.39403955835876	35.0	33.0	35.0	30.0	35.0
100	32.21919110499189	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	38.0
21	223.0
22	986.0
23	3568.0
24	9276.0
25	20041.0
26	36566.0
27	57080.0
28	82550.0
29	110923.0
30	141205.0
31	176565.0
32	220391.0
33	282465.0
34	384719.0
35	548198.0
36	887519.0
37	1599413.0
38	2052553.0
39	1060649.0
40	48813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.741255344233224	19.529668634882608	12.909772019949905	24.81930400093426
2	15.739059777470674	21.06334154437497	39.20490135227129	23.99269732588306
3	18.84683934453567	25.03725523858501	29.98713318640981	26.128772230469515
4	13.094578718668377	17.030868182356073	37.5900502165659	32.28450288240965
5	13.716038823648525	37.37530985612422	35.271240146944244	13.637411173283015
6	31.678410967942238	36.62292882236159	18.071645546086057	13.627014663610119
7	27.77305209156282	31.80702731550209	22.461523203896142	17.958397389038947
8	25.535925184489493	35.7313407250834	20.818835834321845	17.913898256105263
9	26.050623622268258	15.967057953485265	20.77728863688682	37.205029787359656
10-11	25.067111217795095	26.09612615432978	28.38179168501916	20.454970942855965
12-13	25.407477149041412	23.581219527415843	28.528158486827504	22.48314483671524
14-15	22.50506425250131	25.02314938891051	25.90715696761649	26.56462939097169
16-17	22.529288249510017	28.093246463568327	26.660735606789764	22.716729680131895
18-19	22.560620196469433	27.50361029479737	27.979797626695646	21.955971882037552
20-21	23.259596785272613	26.758854400206346	27.799611320922313	22.18193749359873
22-23	23.01894640172722	26.64929441607341	27.660849140346176	22.670910041853194
24-25	22.767125548000568	26.75245236285399	27.83574145550402	22.64468063364142
26-27	22.76424686886328	26.92471439009253	27.71439961680032	22.596639124243865
28-29	22.71534918489115	27.023111004825395	27.702722856766393	22.55881695351706
30-31	22.929327027263238	26.83148568771827	27.61816977329533	22.621017511723164
32-33	22.694775717413073	27.02092663542449	27.55675734382078	22.727540303341655
34-35	22.846777432324945	27.036102640680937	27.56595317730571	22.551166749688413
36-37	22.868406958751418	26.929899988959072	27.519076912428954	22.682616139860553
38-39	22.61045929910205	27.060085312667955	27.543381257979476	22.786074130250523
40-41	23.062211104428243	26.8264489018205	27.604035857338694	22.507304136412568
42-43	22.680835833612896	26.883981711983473	27.457077145034415	22.978105309369216
44-45	22.6506995337854	26.9896825012216	27.38026000269166	22.97935796230134
46-47	22.763093251796505	26.88172430017997	27.494081100038443	22.861101347985084
48-49	22.696990325239522	27.225910401422183	27.24757135887434	22.829527914463956
50-51	22.59444168734491	27.309670329670325	27.033888691953205	23.061999291031547
52-53	22.635925920312054	27.070343196852885	26.94386026560846	23.3498706172266
54-55	22.87278841648397	26.69426478920769	27.407966830950027	23.024979963358312
56-57	22.583909524697596	26.30444792574676	27.613444073563194	23.498198475992453
58-59	22.315826818613452	26.0702815324873	27.677843494124538	23.936048154774713
60-61	22.590861289649975	26.45080591493521	28.093547768931554	22.86478502648326
62-63	22.016215264021287	26.742307918897545	28.057674648003616	23.183802169077552
64-65	21.71532645787053	27.25100045573176	27.569591466443118	23.464081619954595
66-67	21.79543889071906	28.108322601898717	26.856690425563773	23.239548081818455
68-69	21.977971931845456	28.12527941927244	26.622468641662174	23.27428000721993
70-71	21.96644323554249	27.4321366342929	26.555127337578778	24.046292792585838
72-73	22.31341831292692	26.623720577424443	26.534146859869466	24.528714249779167
74-75	22.350721501514066	26.689814608097045	26.90985334623182	24.049610544157073
76-77	22.868098584566155	26.589713855110464	25.924881099359748	24.617306460963633
78-79	23.11939340209853	26.670757544807095	25.300673451676403	24.909175601417974
80-81	22.877125108670036	27.22150322756295	25.570139044069652	24.331232619697364
82-83	22.853470045688255	26.844174814450017	25.71900619347517	24.58334894638656
84-85	22.91983285898427	27.490722872675118	25.572663647032712	24.016780621307902
86-87	23.68762681327918	27.29719348649105	25.681398537319705	23.333781162910068
88-89	23.16606611431954	27.521339650072797	26.254342233270567	23.058252002337095
90-91	23.16671077200131	26.925946059428103	25.99627805012438	23.911065118446203
92-93	23.195803533557108	26.2209850172331	26.39025911518476	24.192952334025026
94-95	22.073180023880866	26.945564981844004	27.377761022309084	23.603493971966046
96-97	22.1227878805754	26.697613306230444	27.7069881590571	23.472610654137053
98-99	23.444884134418434	28.568847848029634	28.05430499655803	19.931963020993905
100	30.486272657169827	40.39377052866806	29.119956814162112	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1525.0
1	1353.0
2	1744.5
3	2461.0
4	3170.0
5	4911.0
6	6560.0
7	7700.5
8	8431.0
9	8372.0
10	7697.5
11	6993.5
12	7384.0
13	9188.5
14	13619.0
15	19765.5
16	24898.0
17	28103.0
18	29369.5
19	29022.0
20	27640.0
21	26180.0
22	25956.0
23	28448.0
24	33612.0
25	41446.5
26	52569.5
27	65519.5
28	79463.5
29	95151.0
30	110162.0
31	124074.0
32	139247.5
33	155542.0
34	171386.0
35	187246.5
36	205438.0
37	222653.5
38	236895.5
39	249672.5
40	263376.0
41	276959.5
42	287646.5
43	297044.0
44	308603.0
45	320658.0
46	330618.0
47	340532.0
48	347693.5
49	350263.5
50	350605.0
51	347415.0
52	337968.5
53	324841.5
54	310181.0
55	291799.0
56	270312.5
57	246951.5
58	221779.0
59	193741.0
60	164271.5
61	136864.0
62	112436.5
63	90355.5
64	70731.5
65	55455.0
66	43858.5
67	33650.5
68	24881.0
69	18764.0
70	13912.0
71	9990.5
72	7235.0
73	5090.5
74	3451.0
75	2391.5
76	1655.5
77	1084.5
78	726.0
79	494.0
80	329.5
81	219.0
82	156.0
83	109.0
84	72.5
85	48.0
86	30.0
87	19.5
88	12.5
89	7.5
90	6.0
91	5.0
92	3.5
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.004907127116603181
22-23	0.004473757591872756
24-25	0.0036004295131092548
26-27	0.017348010086721263
28-29	0.021977118642859117
30-31	0.03865451373777911
32-33	0.023990749386023216
34-35	0.01232241433417615
36-37	0.0068877468631091615
38-39	0.0024628221724892936
40-41	0.007559468937272463
42-43	0.009960478389478491
44-45	0.005392594552891716
46-47	0.0018964483813051717
48-49	0.009837914059514334
50-51	0.004714417930668281
52-53	0.0
54-55	2.493885882489665E-4
56-57	4.85141333799069E-4
58-59	3.429164184094679E-4
60-61	6.274674979048356E-4
62-63	7.665248522687603E-4
64-65	0.0
66-67	2.620459725967296E-4
68-69	4.7059866526074056E-4
70-71	2.1548093536272254E-4
72-73	6.317855523279894E-4
74-75	2.3708459913459378E-4
76-77	0.0
78-79	8.122192868617196E-6
80-81	3.626786223124999E-4
82-83	0.0029022708387437266
84-85	6.787723451856035E-5
86-87	7.147997918985281E-4
88-89	2.0129227892703517E-4
90-91	0.010064088650905354
92-93	9.161635099693557E-4
94-95	2.7774897418045538E-5
96-97	2.3785534399115558E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	200.0
20-21	508.0
22-23	1174.0
24-25	2540.0
26-27	4533.0
28-29	11386.0
30-31	24494.0
32-33	32426.0
34-35	45102.0
36-37	66906.0
38-39	41150.0
40-41	75134.0
42-43	80168.0
44-45	90927.0
46-47	89801.0
48-49	84874.0
50-51	43954.0
52-53	9069.0
54-55	8910.0
56-57	9228.0
58-59	47142.0
60-61	84639.0
62-63	82357.0
64-65	86253.0
66-67	90853.0
68-69	90510.0
70-71	88306.0
72-73	83567.0
74-75	84298.0
76-77	85120.0
78-79	89565.0
80-81	89497.0
82-83	84824.0
84-85	85982.0
86-87	91238.0
88-89	97693.0
90-91	101324.0
92-93	107163.0
94-95	128020.0
96-97	317583.0
98-99	1721295.0
100-101	3264033.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.2997957101682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57436139479987	78.16548884950173
2	6.681491948289767	11.53222780353081
3	1.5520685337876947	4.01829592182175
4	0.5518054528030879	1.9048279139465336
5	0.2414470804492161	1.0418416858791948
6	0.14398221843655792	0.7455381622183057
7	0.07634296386692338	0.4611867529936967
8	0.043120667469279624	0.29770438347879413
9	0.028474246305114453	0.221158947521915
>10	0.10456013038540725	1.3836261069005382
>50	0.0018679090015304536	0.10805830322009674
>100	4.3201477646630763E-4	0.08028186160509253
>500	2.271981491462958E-5	0.01707783100949373
>1k	2.2719814273009863E-5	0.022685476371961076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5894171040839508E-5
2	0.0	7.768251312251853E-5	0.0	1.2947085520419754E-5	2.5894171040839508E-5
3	0.0	7.768251312251853E-5	0.0	5.1788342081679016E-5	2.5894171040839508E-5
4	0.0	1.2947085520419754E-4	0.0	5.1788342081679016E-5	2.5894171040839508E-5
5	0.0	1.424179407246173E-4	0.0	5.1788342081679016E-5	2.5894171040839508E-5
6	0.0	1.424179407246173E-4	0.0	6.473542760209877E-5	6.473542760209877E-5
7	0.0	1.424179407246173E-4	0.0	7.768251312251853E-5	6.473542760209877E-5
8	0.0	1.9420628280629633E-4	0.0	9.062959864293828E-5	6.473542760209877E-5
9	0.0	2.848358814492346E-4	0.0	9.062959864293828E-5	9.062959864293828E-5
10-11	1.2947085520419754E-5	2.848358814492346E-4	0.0	1.2299731244398766E-4	9.062959864293828E-5
12-13	2.5894171040839508E-5	2.848358814492346E-4	0.0	1.2947085520419754E-4	9.062959864293828E-5
14-15	2.5894171040839508E-5	4.531479932146914E-4	0.0	1.5536502624503707E-4	1.2299731244398766E-4
16-17	2.5894171040839508E-5	4.660950787351112E-4	0.0	1.683121117654568E-4	1.5536502624503707E-4
18-19	2.5894171040839508E-5	4.919892497759507E-4	0.0	2.3952108212776544E-4	1.8125919728587657E-4
20-21	2.5894171040839508E-5	5.178834208167902E-4	0.0	2.977829669696544E-4	1.8125919728587657E-4
22-23	2.5894171040839508E-5	6.020394766995186E-4	0.0	3.754654800921729E-4	2.2010045384713583E-4
24-25	2.5894171040839508E-5	7.574045029445557E-4	0.0	4.531479932146914E-4	2.7836233868902473E-4
26-27	3.2367713801049386E-5	8.156663877864446E-4	0.0	6.861955325822471E-4	2.848358814492346E-4
28-29	3.884125656125927E-5	8.350870160670742E-4	0.0	0.0018384861438996052	2.848358814492346E-4
30-31	6.473542760209877E-5	9.516107857508521E-4	0.0	0.005625508658622384	2.848358814492346E-4
32-33	6.473542760209877E-5	9.775049567916916E-4	0.0	0.0126493025534501	2.977829669696544E-4
34-35	6.473542760209877E-5	0.0011199228975163089	0.0	0.021291482138330285	3.366242235309136E-4
36-37	6.473542760209877E-5	0.0012429202099602966	0.0	0.039864076317372425	3.366242235309136E-4
38-39	7.120897036230865E-5	0.001268814381001136	0.0	0.28919904926961604	3.430977662911235E-4
40-41	7.768251312251853E-5	0.0013594439796440743	0.0	0.5495584655424971	3.4957130905133337E-4
42-43	7.768251312251853E-5	0.0013982852362053335	0.0	0.5929312020359033	3.4957130905133337E-4
44-45	7.768251312251853E-5	0.0015730708907310002	0.0	0.6507464124273377	3.5604485181154325E-4
46-47	7.768251312251853E-5	0.0017090152886954076	0.0	0.7144719673588438	3.754654800921729E-4
48-49	7.768251312251853E-5	0.0017090152886954076	0.0	0.7740285607527746	3.8841256561259266E-4
50-51	7.768251312251853E-5	0.001974430541864013	0.0	0.8282185871984915	3.8841256561259266E-4
52-53	7.768251312251853E-5	0.0019938511701446423	0.0	1.5333427588115922	4.013596511330124E-4
54-55	7.768251312251853E-5	0.0020003247129048523	0.0	2.4795351892721484	4.1430673665343213E-4
56-57	7.768251312251853E-5	0.0020715336832671607	0.0	3.5042840611278514	4.1430673665343213E-4
58-59	7.768251312251853E-5	0.0020780072260273706	0.0	4.5355453170003255	4.272538221738519E-4
60-61	9.062959864293828E-5	0.0021427426536294696	0.0	4.812153325601334	4.272538221738519E-4
62-63	9.062959864293828E-5	0.0021880574529509387	0.0	4.823287819148895	4.272538221738519E-4
64-65	9.062959864293828E-5	0.0023952108212776544	0.0	4.8334901225389855	4.337273649340618E-4
66-67	9.062959864293828E-5	0.002427578535078704	0.0	4.842158196294907	4.4020090769427165E-4
68-69	9.062959864293828E-5	0.0024664197916399633	0.0	4.850373122057613	4.4020090769427165E-4
70-71	9.062959864293828E-5	0.0025052610482012225	0.0	4.857286865725517	4.531479932146914E-4
72-73	9.062959864293828E-5	0.0025117345909614324	0.0	4.862057866739792	4.919892497759507E-4
74-75	9.062959864293828E-5	0.002537628762002272	0.0	4.866654082099541	4.919892497759507E-4
76-77	9.062959864293828E-5	0.0025958906468441607	0.0	4.87094604094956	4.919892497759507E-4
78-79	1.0357668416335803E-4	0.0026153112751247905	0.0	4.873613140566766	4.919892497759507E-4
80-81	1.0357668416335803E-4	0.0026217848178850004	0.0	4.8744935423821545	5.24356963577E-4
82-83	1.0357668416335803E-4	0.0026282583606452104	0.0	4.875289788141661	5.567246773780495E-4
84-85	1.0357668416335803E-4	0.0026541525316860497	0.0	4.875483994424467	5.696717628984692E-4
86-87	1.0357668416335803E-4	0.002692993788247309	0.0	4.875606991736911	5.696717628984692E-4
88	1.0357668416335803E-4	0.002744782130328988	0.0	4.875613465279671	5.696717628984692E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	10435	0.0	59.570847	94
TATCTCG	12940	0.0	52.69754	94
GGTATCA	6850	0.0	45.824924	1
CCGTCTT	5655	0.0	34.92668	90-91
GTATCAA	11855	0.0	34.204117	1
ATGCCGT	5975	0.0	32.919643	88-89
TATGCCG	5715	0.0	32.89841	86-87
TGCCGTC	5960	0.0	32.55246	88-89
GCCGTCT	6240	0.0	32.241184	90-91
CGTATGC	6170	0.0	30.23855	84-85
TCGTATG	6510	0.0	29.53974	84-85
CTCGTAT	6320	0.0	29.180906	82-83
CTATCTC	15825	0.0	28.856016	92-93
TCTCGTA	6510	0.0	28.502241	82-83
TCAACGC	14120	0.0	27.891857	4
ATCAACG	14265	0.0	27.549976	3
CGCTATC	16565	0.0	27.00508	92-93
CAACGCA	14725	0.0	26.717604	5
TATCAAC	15175	0.0	26.391699	2
GTATGCC	7420	0.0	26.283175	86-87
>>END_MODULE