FastQCFastQC Report
Fri 27 May 2016
SRR2140225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6361918
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65600.043.405921
GTATCAA119100.035.164791
TCAACGC136100.029.948984
ATCAACG137150.029.7499563
CAACGCA139400.029.2995515
AACGCAG144700.028.1403266
TATCAAC148550.027.8021972
ACGCAGA168150.024.2405937
CGCAGAG172000.023.7221348
GTACATG165500.020.4905071
TACATGG165950.019.9597112
GCAGAGT206750.019.554299
ACATGGG169850.018.8904633
GAGTACT116050.017.97295812-13
AGAGTAC181650.017.69761310-11
GTGGTAT38500.017.6812021
CATGGGG119650.017.3107744
CAGAGTA199450.017.11711310-11
GGACCGA8550.015.5350466
GTACTTT134450.015.49821914-15
Produced by FastQC (version 0.11.5)