##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6361918
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36153169531578	33.0	31.0	34.0	31.0	34.0
2	32.64923706970131	34.0	31.0	34.0	31.0	34.0
3	32.32009890727922	33.0	31.0	34.0	30.0	34.0
4	36.18675374313218	37.0	35.0	37.0	35.0	37.0
5	36.07149636320368	37.0	35.0	37.0	35.0	37.0
6	36.16387290750997	37.0	36.0	37.0	35.0	37.0
7	36.143474499356955	37.0	36.0	37.0	35.0	37.0
8	36.14777037365147	37.0	36.0	37.0	35.0	37.0
9	37.96948011590215	39.0	38.0	39.0	35.0	39.0
10-11	37.89763967407313	39.0	38.0	39.0	35.0	39.0
12-13	37.86517509342308	39.0	38.0	39.0	35.0	39.0
14-15	39.25305473915256	41.0	39.0	41.0	36.0	41.0
16-17	39.24220620259488	41.0	39.0	41.0	36.0	41.0
18-19	39.216443924615184	40.5	39.0	41.0	36.0	41.0
20-21	39.21180365473096	40.0	39.0	41.0	36.0	41.0
22-23	39.15066115119272	40.0	39.0	41.0	36.0	41.0
24-25	39.09915952126339	40.0	39.0	41.0	36.0	41.0
26-27	38.94493399513532	40.0	38.5	41.0	35.0	41.0
28-29	38.82725657116006	40.0	38.0	41.0	35.0	41.0
30-31	38.69905681270387	40.0	38.0	41.0	35.0	41.0
32-33	38.54406945945249	40.0	38.0	41.0	34.0	41.0
34-35	38.63732498577839	40.0	38.0	41.0	35.0	41.0
36-37	38.627975241999245	40.0	38.0	41.0	35.0	41.0
38-39	38.58688411684378	40.0	38.0	41.0	34.0	41.0
40-41	38.522289856568754	40.0	38.0	41.0	34.0	41.0
42-43	38.495808941350376	40.0	38.0	41.0	34.0	41.0
44-45	38.547544149293685	40.0	38.0	41.0	34.0	41.0
46-47	38.50354848022073	40.0	38.0	41.0	34.0	41.0
48-49	38.42968414332152	40.0	38.0	41.0	34.0	41.0
50-51	38.34640447901474	40.0	38.0	41.0	34.0	41.0
52-53	38.15923709886151	40.0	37.5	41.0	33.0	41.0
54-55	38.06260078812386	40.0	37.0	41.0	33.0	41.0
56-57	37.93230968764665	40.0	37.0	41.0	33.0	41.0
58-59	37.75832579384881	39.5	36.5	41.0	33.0	41.0
60-61	37.57676713437104	39.0	36.0	41.0	33.0	41.0
62-63	37.30804101985131	39.0	35.5	41.0	32.0	41.0
64-65	37.005839758140986	38.5	35.0	40.0	32.0	41.0
66-67	36.73582993682596	38.0	35.0	40.0	31.0	41.0
68-69	36.45393989543524	37.5	35.0	40.0	31.0	41.0
70-71	36.14798448793296	37.0	35.0	39.5	31.0	41.0
72-73	35.80906616918554	36.5	34.5	39.0	31.0	41.0
74-75	35.46020219067829	36.0	34.0	39.0	31.0	40.0
76-77	34.25697395932477	35.0	33.0	37.0	29.5	39.0
78-79	34.86121067793703	35.0	34.0	37.0	30.5	39.0
80-81	34.708523158978515	35.0	34.0	37.0	31.0	39.0
82-83	34.46122818917165	35.0	34.0	36.5	30.0	39.0
84-85	34.24736934953513	35.0	34.0	36.0	30.0	37.0
86-87	34.01108176572121	35.0	34.0	36.0	30.0	37.0
88-89	33.83813687166561	35.0	34.0	35.0	30.0	37.0
90-91	33.66197056778382	35.0	34.0	35.0	30.0	36.0
92-93	33.540643213098136	35.0	34.0	35.0	30.0	36.0
94-95	33.50483137513487	35.0	34.0	35.0	30.0	36.0
96-97	33.51706804572776	35.0	33.5	35.0	30.0	35.5
98-99	33.572947434850164	35.0	33.0	35.0	30.0	35.0
100	32.439327388535034	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	20.0
21	143.0
22	614.0
23	2165.0
24	5734.0
25	12411.0
26	23083.0
27	37093.0
28	54375.0
29	74752.0
30	96183.0
31	122465.0
32	154167.0
33	200346.0
34	277023.0
35	400965.0
36	660483.0
37	1271296.0
38	1824306.0
39	1093971.0
40	50317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.683341407418325	20.399256953641967	13.937070550107688	23.980331088832017
2	14.825183223046887	21.21963848009358	40.948122877408984	23.007055419450552
3	18.028258144792183	25.995305189409862	30.684032708375053	25.292403957422906
4	12.4776207426754	17.84551451307609	39.01708572792042	30.65977901632809
5	13.100593248765547	37.87623480843356	35.94961456592179	13.07355737687911
6	30.183680456114022	37.98321512474697	18.682070407069062	13.151034012069946
7	26.541728453589	32.598313904706096	23.318219442627207	17.5417381990777
8	24.41001282946432	36.777729609215335	21.31880668691423	17.493450874406115
9	25.277015516389866	16.795909661205943	21.582217815444967	36.344857006959224
10-11	24.036186885778786	26.84553620464772	29.19041081636073	19.92786609321277
12-13	24.674044839936634	24.377412597898935	29.325503723876984	21.62303883828745
14-15	21.807983064226857	25.941021245479746	26.78757412465863	25.463421565634764
16-17	21.557932686337676	29.05545937561597	27.535697567934704	21.850910370111656
18-19	21.569397816936675	28.34564310017501	29.052901922480224	21.032057160408094
20-21	22.2541255305629	27.545627132125045	28.969856207188027	21.230391130124026
22-23	22.020247243783672	27.475764640116118	28.7698734745732	21.73411464152701
24-25	21.82225280397205	27.567437396996908	28.880232038327964	21.730077760703086
26-27	21.801817322222668	27.767530331314	28.784521216383435	21.6461311300799
28-29	21.71041911510611	27.801501455878498	28.782713971631818	21.705365457383575
30-31	21.993678208995657	27.694556780416907	28.67118526524881	21.640579745338623
32-33	21.77790445105613	27.840559957750738	28.634410216122575	21.74712537507056
34-35	21.91094295586417	27.86488311631005	28.641208305129553	21.58296562269623
36-37	21.876264076561075	27.7692698035436	28.610190688480387	21.74427543141494
38-39	21.735520847883432	27.905719464213814	28.689613423137644	21.669146264765107
40-41	21.98746625807015	27.737372464606803	28.53294049947016	21.74222077785289
42-43	21.883188873211047	27.87035958397579	28.346369539551358	21.900082003261804
44-45	21.93553282148335	27.975063967472668	28.212304401031545	21.877098810012434
46-47	21.957145146677284	27.93171688699325	28.0678313211009	22.043306645228565
48-49	21.90864058289841	28.005544569188327	28.05360088401975	22.032213963893508
50-51	21.90981755920376	28.134930522804698	27.973876721489937	21.981375196501613
52-53	22.039287185415763	28.043307358541377	27.8576647279778	22.059740728065062
54-55	22.016876537448525	27.93770564781734	27.93473727698373	22.110680537750408
56-57	22.00861504792197	28.040652752170914	27.880499415928956	22.07023278397816
58-59	21.921856446045577	28.084608121809175	27.923322933708032	22.07021249843721
60-61	21.998834896992665	28.082075034072073	27.926871806305222	21.99221826263004
62-63	22.011590657040735	28.116656336037472	27.861043678845178	22.010709328076615
64-65	21.913614093133134	28.10442407099322	27.82988394774223	22.152077888131423
66-67	21.96861858236832	28.171275245520448	27.710380188937517	22.14972598317371
68-69	21.982704978012244	28.156754468125257	27.75911326161456	22.101427292247944
70-71	21.949428267661347	27.949153399549942	27.814319447604625	22.287098885184083
72-73	22.016085060109578	27.975659493536796	27.720776804248764	22.28747864210486
74-75	21.928871665137027	28.140871461900396	27.67033575101055	22.259921121952022
76-77	22.06460411843654	28.0423132613088	27.627002763603514	22.266079856651146
78-79	22.01761373105982	28.039399393106418	27.59517192568788	22.34781495014588
80-81	22.019332460486314	28.077814156535748	27.634564985189904	22.26828839778804
82-83	22.0768858334117	28.091997222310056	27.688772243797732	22.14234470048051
84-85	22.164296689493394	28.0813170463193	27.61464782778559	22.139738436401718
86-87	22.002106428296614	28.099097656221584	27.68123871226778	22.217557203214028
88-89	22.039552451036094	28.082056207038153	27.698945066824848	22.17944627510091
90-91	22.081754947379043	28.16293541898625	27.680425781785527	22.07488385184918
92-93	22.158707872623907	28.091972906835398	27.69777107762213	22.051548142918566
94-95	21.957105228342616	28.19277246920239	27.80484841955658	22.045273882898414
96-97	22.00637333006496	28.225423019376684	27.917394149764007	21.850809500794355
98-99	23.04701612280142	30.198516283964942	28.128655658483552	18.625811934750082
100	22.03483741200134	31.265973851827024	21.861991283942338	24.837197452229297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1967.0
1	1605.0
2	1761.0
3	2376.5
4	3025.0
5	4622.0
6	6068.0
7	7278.5
8	8286.0
9	8299.5
10	7603.0
11	6872.0
12	7244.5
13	9248.5
14	13819.0
15	20178.0
16	25530.0
17	28961.0
18	30386.5
19	30074.0
20	28973.5
21	28362.5
22	29210.0
23	32538.5
24	38863.5
25	48535.0
26	60894.0
27	74400.5
28	88852.0
29	104729.0
30	119839.0
31	133834.5
32	148872.5
33	163046.0
34	177187.0
35	190221.5
36	203671.0
37	216825.0
38	227476.5
39	237220.5
40	246175.0
41	253785.5
42	257651.5
43	261105.5
44	264041.5
45	267539.5
46	270248.5
47	270258.5
48	269751.5
49	266918.0
50	262192.5
51	254190.5
52	243204.0
53	229978.0
54	214942.5
55	198971.5
56	183264.0
57	166489.0
58	147693.0
59	128321.0
60	107467.0
61	87331.5
62	69832.0
63	54703.5
64	41730.0
65	31622.5
66	24024.5
67	18482.0
68	14089.5
69	10351.0
70	7557.0
71	5429.0
72	3879.5
73	2647.5
74	1757.5
75	1174.5
76	799.5
77	527.0
78	339.0
79	228.0
80	164.0
81	111.5
82	74.0
83	52.0
84	35.5
85	23.0
86	16.0
87	10.5
88	6.0
89	3.0
90	2.5
91	3.0
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.029929653290093E-4
20-21	0.0
22-23	6.052269601100413E-4
24-25	0.0063442972275735586
26-27	0.02831929728506727
28-29	0.027315349691956675
30-31	0.028911258594261777
32-33	0.04986853881703353
34-35	0.02337500288617016
36-37	0.01906848306068461
38-39	0.01518809600613377
40-41	0.008115810425694932
42-43	0.01073191672032625
44-45	0.004735658125484361
46-47	0.00356137116133722
48-49	0.008293338335825422
50-51	0.002007366265415505
52-53	0.0020694555857322418
54-55	0.001140328870846352
56-57	0.006649523327688861
58-59	0.00467703391400873
60-61	0.007811693070758567
62-63	0.006014481271886534
64-65	0.0013592729138972247
66-67	0.0041258334601748355
68-69	0.01155953234402297
70-71	0.015055476717719429
72-73	0.004847972217553091
74-75	0.0015408297953573237
76-77	0.002655342207967389
78-79	0.0028993119932639987
80-81	0.003434665419514999
82-83	0.003500735924500727
84-85	0.004243685749245601
86-87	3.163120878453495E-5
88-89	2.888812495847332E-4
90-91	0.004239894998456787
92-93	6.966992710866817E-4
94-95	0.0021410641223913386
96-97	4.046909461963387E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	90.0
20-21	406.0
22-23	935.0
24-25	2001.0
26-27	3384.0
28-29	7597.0
30-31	16900.0
32-33	22263.0
34-35	30688.0
36-37	46217.0
38-39	51473.0
40-41	52564.0
42-43	61016.0
44-45	68102.0
46-47	67825.0
48-49	62428.0
50-51	56143.0
52-53	53726.0
54-55	58140.0
56-57	61522.0
58-59	60917.0
60-61	59718.0
62-63	58570.0
64-65	62497.0
66-67	64403.0
68-69	65489.0
70-71	63980.0
72-73	60554.0
74-75	61731.0
76-77	62883.0
78-79	66330.0
80-81	65254.0
82-83	63211.0
84-85	64059.0
86-87	67978.0
88-89	72475.0
90-91	77404.0
92-93	81142.0
94-95	97835.0
96-97	227997.0
98-99	405321.0
100-101	3728750.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.9668082783158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.95907128864216	81.81305064788602
2	6.023264854283067	10.717412990010388
3	1.2153080310695246	3.243662297977795
4	0.3913076671990267	1.3925357682212323
5	0.16816745513552836	0.7480660869847413
6	0.08224832703058807	0.4390422685285529
7	0.05097766645875426	0.31747241948183436
8	0.023675732026011226	0.16850834496055375
9	0.022763008580550005	0.18226369982011048
>10	0.061650026027323654	0.834732547037858
>50	0.0011332833037696873	0.0655851546387213
>100	4.3266024374357575E-4	0.07766777445222398
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.571853016653154E-5	0.0
2	0.0	0.0	0.0	3.143706033306308E-5	0.0
3	0.0	0.0	0.0	6.287412066612616E-5	0.0
4	0.0	0.0	0.0	9.431118099918924E-5	0.0
5	0.0	0.0	0.0	9.431118099918924E-5	0.0
6	4.715559049959462E-5	0.0	0.0	9.431118099918924E-5	0.0
7	4.715559049959462E-5	0.0	0.0	9.431118099918924E-5	0.0
8	4.715559049959462E-5	0.0	0.0	9.431118099918924E-5	0.0
9	4.715559049959462E-5	3.143706033306308E-5	0.0	9.431118099918924E-5	0.0
10-11	5.501485558286039E-5	3.929632541632885E-5	0.0	1.571853016653154E-4	0.0
12-13	6.287412066612616E-5	4.715559049959462E-5	0.0	1.7290383183184694E-4	0.0
14-15	6.287412066612616E-5	4.715559049959462E-5	0.0	1.7290383183184694E-4	7.85926508326577E-6
16-17	9.431118099918924E-5	4.715559049959462E-5	0.0	1.8862236199837848E-4	1.571853016653154E-5
18-19	9.431118099918924E-5	5.501485558286039E-5	0.0	2.122001572481758E-4	1.571853016653154E-5
20-21	9.431118099918924E-5	6.287412066612616E-5	0.0	2.2005942233144156E-4	1.571853016653154E-5
22-23	9.431118099918924E-5	6.287412066612616E-5	0.0	2.7507427791430197E-4	1.571853016653154E-5
24-25	9.431118099918924E-5	7.85926508326577E-5	0.0	3.851039890800227E-4	1.571853016653154E-5
26-27	9.431118099918924E-5	7.85926508326577E-5	0.0	5.029929653290093E-4	1.571853016653154E-5
28-29	9.431118099918924E-5	9.431118099918924E-5	3.143706033306308E-5	0.001265341678405789	1.571853016653154E-5
30-31	9.431118099918924E-5	1.2574824133225232E-4	3.143706033306308E-5	0.003961069601965948	1.571853016653154E-5
32-33	9.431118099918924E-5	1.2574824133225232E-4	3.143706033306308E-5	0.009462555160251986	1.571853016653154E-5
34-35	9.431118099918924E-5	1.571853016653154E-4	3.143706033306308E-5	0.016960294049687533	1.571853016653154E-5
36-37	9.431118099918924E-5	1.8862236199837848E-4	3.143706033306308E-5	0.028474117396671883	1.571853016653154E-5
38-39	9.431118099918924E-5	1.9648162708164424E-4	3.143706033306308E-5	0.05590295253726942	1.571853016653154E-5
40-41	9.431118099918924E-5	2.357779524979731E-4	3.143706033306308E-5	0.08463642568168908	1.571853016653154E-5
42-43	9.431118099918924E-5	2.357779524979731E-4	3.143706033306308E-5	0.10444963295660209	1.571853016653154E-5
44-45	1.1788897624898655E-4	2.907928080808335E-4	3.143706033306308E-5	0.12884479177505903	1.571853016653154E-5
46-47	1.4146677149878386E-4	3.458076636636939E-4	3.143706033306308E-5	0.154245936524174	1.571853016653154E-5
48-49	1.4146677149878386E-4	3.458076636636939E-4	3.143706033306308E-5	0.17798091707563662	1.571853016653154E-5
50-51	1.4146677149878386E-4	3.615261938302254E-4	3.143706033306308E-5	0.20118932686652044	1.571853016653154E-5
52-53	1.4146677149878386E-4	3.6938545891349115E-4	3.143706033306308E-5	0.2515907938455038	1.571853016653154E-5
54-55	1.4146677149878386E-4	3.851039890800227E-4	3.143706033306308E-5	0.31495218894679244	1.571853016653154E-5
56-57	1.4146677149878386E-4	3.929632541632885E-4	3.143706033306308E-5	0.37523275213544094	1.571853016653154E-5
58-59	1.4146677149878386E-4	3.929632541632885E-4	3.143706033306308E-5	0.41871806584115046	1.571853016653154E-5
60-61	1.571853016653154E-4	4.008225192465543E-4	3.143706033306308E-5	0.44947137011196936	1.571853016653154E-5
62-63	1.7290383183184694E-4	4.0868178432982004E-4	3.143706033306308E-5	0.47333209890476424	1.571853016653154E-5
64-65	1.7290383183184694E-4	4.479781097461489E-4	3.143706033306308E-5	0.48538821154249395	1.571853016653154E-5
66-67	1.8862236199837848E-4	4.5583737482941464E-4	3.143706033306308E-5	0.49649335310514847	1.571853016653154E-5
68-69	1.8862236199837848E-4	4.6369663991268045E-4	3.143706033306308E-5	0.5065689309418953	1.571853016653154E-5
70-71	2.2005942233144156E-4	4.8727443516247777E-4	3.143706033306308E-5	0.5132964618531708	1.571853016653154E-5
72-73	2.2005942233144156E-4	5.10852230412275E-4	3.143706033306308E-5	0.5184128434223767	1.571853016653154E-5
74-75	2.2005942233144156E-4	5.265707605788066E-4	3.143706033306308E-5	0.5229633579055876	3.143706033306308E-5
76-77	2.2005942233144156E-4	5.344300256620723E-4	3.143706033306308E-5	0.5271995017854678	4.715559049959462E-5
78-79	2.2005942233144156E-4	5.344300256620723E-4	3.143706033306308E-5	0.5296358739612803	5.501485558286039E-5
80-81	2.2005942233144156E-4	5.344300256620723E-4	3.143706033306308E-5	0.5304453782648566	8.645191591592347E-5
82-83	2.2005942233144156E-4	5.422892907453381E-4	3.143706033306308E-5	0.5309483712301857	9.431118099918924E-5
84-85	2.2005942233144156E-4	5.737263510784013E-4	3.143706033306308E-5	0.531286319628766	1.0217044608245501E-4
86-87	2.2005942233144156E-4	5.973041463281985E-4	3.143706033306308E-5	0.5313884900748485	1.1002971116572078E-4
88	2.2005942233144156E-4	6.287412066612616E-4	3.143706033306308E-5	0.5314120678700983	1.1002971116572078E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6560	0.0	43.40592	1
GTATCAA	11910	0.0	35.16479	1
TCAACGC	13610	0.0	29.94898	4
ATCAACG	13715	0.0	29.749956	3
CAACGCA	13940	0.0	29.299551	5
AACGCAG	14470	0.0	28.140326	6
TATCAAC	14855	0.0	27.802197	2
ACGCAGA	16815	0.0	24.240593	7
CGCAGAG	17200	0.0	23.722134	8
GTACATG	16550	0.0	20.490507	1
TACATGG	16595	0.0	19.959711	2
GCAGAGT	20675	0.0	19.55429	9
ACATGGG	16985	0.0	18.890463	3
GAGTACT	11605	0.0	17.972958	12-13
AGAGTAC	18165	0.0	17.697613	10-11
GTGGTAT	3850	0.0	17.681202	1
CATGGGG	11965	0.0	17.310774	4
CAGAGTA	19945	0.0	17.117113	10-11
GGACCGA	855	0.0	15.535046	6
GTACTTT	13445	0.0	15.498219	14-15
>>END_MODULE