FastQCFastQC Report
Fri 27 May 2016
SRR2140224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434836
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.044.1228641
GTATCAA123300.031.8397881
TCAACGC146900.025.911384
ATCAACG149200.025.539553
CAACGCA150150.025.323095
AACGCAG154100.024.7007246
TATCAAC156200.024.5796222
ACGCAGA179850.021.0954867
GTACATG178700.020.81631
CGCAGAG183450.020.6590568
TACATGG175100.020.5384882
ACATGGG180050.019.5162453
GTGGTAT41750.018.4512371
CATGGGG137300.018.1520294
GCAGAGT218850.017.2608879
AGAGTAC194250.016.99741410-11
GAGTACT124000.016.95955312-13
AGGACCG11000.016.4778425
CAGAGTA210550.016.33697310-11
TGGTATC40100.016.334022
Produced by FastQC (version 0.11.5)