##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6434836
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36226579822703	33.0	31.0	34.0	31.0	34.0
2	32.64853152434654	34.0	31.0	34.0	31.0	34.0
3	32.31847198592163	33.0	31.0	34.0	30.0	34.0
4	36.18587109290742	37.0	35.0	37.0	35.0	37.0
5	36.0723160621343	37.0	35.0	37.0	35.0	37.0
6	36.16177552932196	37.0	36.0	37.0	35.0	37.0
7	36.14021460686799	37.0	36.0	37.0	35.0	37.0
8	36.14354600490207	37.0	36.0	37.0	35.0	37.0
9	37.96553152248169	39.0	38.0	39.0	35.0	39.0
10-11	37.894045629134915	39.0	38.0	39.0	35.0	39.0
12-13	37.85912352700208	39.0	38.0	39.0	35.0	39.0
14-15	39.243244039164324	41.0	39.0	41.0	36.0	41.0
16-17	39.23442446707267	41.0	39.0	41.0	36.0	41.0
18-19	39.208031875249034	41.0	39.0	41.0	36.0	41.0
20-21	39.199388142063114	40.5	39.0	41.0	36.0	41.0
22-23	39.139489609151575	40.0	39.0	41.0	36.0	41.0
24-25	39.085673395336734	40.0	39.0	41.0	35.5	41.0
26-27	38.93355938655567	40.0	38.0	41.0	35.0	41.0
28-29	38.81495159832539	40.0	38.0	41.0	35.0	41.0
30-31	38.684320676227884	40.0	38.0	41.0	35.0	41.0
32-33	38.52842319038451	40.0	38.0	41.0	34.0	41.0
34-35	38.61543417512493	40.0	38.0	41.0	35.0	41.0
36-37	38.60612741025654	40.0	38.0	41.0	35.0	41.0
38-39	38.56410741806421	40.0	38.0	41.0	34.0	41.0
40-41	38.49579578473494	40.0	38.0	41.0	34.0	41.0
42-43	38.46702233325637	40.0	38.0	41.0	34.0	41.0
44-45	38.52011293130406	40.0	38.0	41.0	34.0	41.0
46-47	38.4752059223012	40.0	38.0	41.0	34.0	41.0
48-49	38.39483451139879	40.0	38.0	41.0	34.0	41.0
50-51	38.303264060277954	40.0	38.0	41.0	34.0	41.0
52-53	38.10898571780207	40.0	37.0	41.0	33.0	41.0
54-55	38.007177250914204	40.0	37.0	41.0	33.0	41.0
56-57	37.86995212379325	40.0	37.0	41.0	33.0	41.0
58-59	37.68763310745938	39.0	36.0	41.0	33.0	41.0
60-61	37.498687814709434	39.0	36.0	41.0	33.0	41.0
62-63	37.22060226581992	39.0	35.0	41.0	32.0	41.0
64-65	36.91220881874723	38.5	35.0	40.0	31.5	41.0
66-67	36.63506086236444	38.0	35.0	40.0	31.0	41.0
68-69	36.34614534185759	37.0	35.0	40.0	31.0	41.0
70-71	36.04186348606814	37.0	35.0	39.0	31.0	41.0
72-73	35.70702071941007	36.0	34.5	39.0	31.0	41.0
74-75	35.36271246988633	36.0	34.0	39.0	30.5	40.0
76-77	34.17772472972365	35.0	32.5	37.0	29.5	39.0
78-79	34.77566793621431	35.0	34.0	37.0	30.0	39.0
80-81	34.634848274337834	35.0	34.0	37.0	30.0	39.0
82-83	34.39815475133918	35.0	34.0	36.0	30.0	38.5
84-85	34.192688437497466	35.0	34.0	36.0	30.0	37.0
86-87	33.971423456542276	35.0	34.0	36.0	30.0	37.0
88-89	33.80595677035152	35.0	34.0	35.0	30.0	36.5
90-91	33.63918067636929	35.0	34.0	35.0	30.0	36.0
92-93	33.52782181860741	35.0	34.0	35.0	30.0	36.0
94-95	33.49480124922751	35.0	33.5	35.0	30.0	36.0
96-97	33.50792966010065	35.0	33.0	35.0	30.0	35.5
98-99	33.56781107372973	35.0	33.0	35.0	30.0	35.0
100	32.48016717541289	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	31.0
21	152.0
22	649.0
23	2242.0
24	6075.0
25	13129.0
26	23912.0
27	38558.0
28	55886.0
29	76193.0
30	98588.0
31	123873.0
32	157523.0
33	203875.0
34	283335.0
35	408436.0
36	675209.0
37	1299228.0
38	1808594.0
39	1108196.0
40	51150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27815596232756	20.050145800141607	13.563065165918758	24.108633071612083
2	15.165794435165092	21.05609218323513	40.52595901434007	23.25215436725971
3	18.250612758429273	25.74858162663353	30.41323819286148	25.587567422075715
4	12.613064264574886	17.512147939745475	38.69080734924713	31.183980446432514
5	13.22246596494456	37.801818103833575	35.753669557390424	13.22204637383144
6	30.58985497066281	37.625543215087376	18.4829419118063	13.301659902443513
7	26.864227775191164	32.46295632087593	23.042638538107266	17.63017736582564
8	24.780274120428246	36.451029987399835	21.080987922613723	17.687707969558197
9	25.597000451915168	16.43658051269683	21.292244277865045	36.67417475752296
10-11	24.41419641464056	26.569822447689422	28.900557838614688	20.11542329905533
12-13	25.011274568613718	24.10456925397943	29.048665731341096	21.83549044606576
14-15	22.12081240298898	25.615454690686757	26.585634816489495	25.678098089834766
16-17	21.86521925345106	28.715059715585607	27.26669335473352	22.153027676229822
18-19	21.844302951889567	28.025976833350413	28.82060784851395	21.30911236624606
20-21	22.600491715892648	27.19906101535291	28.739566189714683	21.460881079039755
22-23	22.29987885492767	27.137125668604	28.558895863186034	22.0040996132823
24-25	22.08173538942855	27.22615801103624	28.665988480578925	22.026118118956287
26-27	22.034909035247686	27.421260923462608	28.54224926300607	22.001580778283632
28-29	22.00527584138353	27.563597581777604	28.520156147220742	21.910970429618125
30-31	22.293628168688148	27.373712108252533	28.414808780501343	21.917850942557973
32-33	22.051223001953495	27.537532221572874	28.404945998235732	22.006298778237902
34-35	22.18875505161465	27.552315835726375	28.41578192414734	21.84314718851164
36-37	22.195261253159288	27.488511881146437	28.32617561334932	21.990051252344955
38-39	22.044393139305825	27.638765594966603	28.38833866127676	21.928502604450813
40-41	22.277585007732323	27.436348781208775	28.284211932354193	22.00185427870471
42-43	22.10974596034856	27.579127117849122	28.104615311709548	22.206511610092775
44-45	22.214260741969127	27.687614811426663	27.96795279526706	22.13017165133715
46-47	22.23039726036041	27.651435524520302	27.78023803807055	22.337929177048743
48-49	22.208314862694394	27.759958769164793	27.758253140792817	22.273473227347996
50-51	22.203766962831526	27.847831019940937	27.664003383284868	22.28439863394266
52-53	22.337778949362814	27.7731297658569	27.520060533419006	22.369030751361283
54-55	22.299979641473257	27.68011684355647	27.59444462693065	22.42545888803963
56-57	22.25938446706362	27.7755290604082	27.594443063089493	22.37064340943869
58-59	22.227901078265898	27.820674407081352	27.609200030339743	22.342224484313007
60-61	22.32454221291883	27.782666281163017	27.629686697022986	22.263104808895168
62-63	22.347807237423282	27.839591559614007	27.538053401452643	22.274547801510064
64-65	22.2567658881467	27.7847909758468	27.55548139159688	22.402961744409616
66-67	22.31030952530883	27.92442215737989	27.31898218420796	22.44628613310331
68-69	22.226917793321995	27.88208519418816	27.46218844416196	22.428808568327884
70-71	22.244312639625953	27.644823627695704	27.47959571809461	22.631268014583732
72-73	22.32060016052686	27.65963490972892	27.434309895801988	22.585455033942235
74-75	22.186693606204024	27.866172051666126	27.372275118205703	22.57485922392415
76-77	22.373606702534513	27.76427581794552	27.29871565287144	22.563401826648523
78-79	22.27443474626143	27.782499371259746	27.292196970946954	22.65086891153187
80-81	22.326305838730928	27.78408971652855	27.29015642758665	22.599448017153872
82-83	22.369781027630765	27.79004674039558	27.415391618638836	22.424780613334818
84-85	22.48933333236085	27.798613815107203	27.30341828289079	22.408634569641155
86-87	22.241079322522133	27.85816258048811	27.41348109279567	22.487277004194087
88-89	22.360751925712194	27.83239040351233	27.337450113701774	22.4694075570737
90-91	22.407172248745404	27.875405769824507	27.349038798659674	22.368383182770415
92-93	22.45906046046297	27.810696904904358	27.396633688124737	22.333608946507933
94-95	22.260314713308553	27.905916660861763	27.489795429112156	22.343973196717528
96-97	22.318651532646832	27.972110521616678	27.609406622549766	22.099831323186724
98-99	23.420382908375007	29.955427202973144	27.742310595465025	18.88187929318682
100	29.860254052783635	41.40596796932334	28.73377797789302	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1790.0
1	1543.0
2	1896.5
3	2687.0
4	3546.5
5	5434.0
6	7146.5
7	8471.5
8	9402.0
9	9365.5
10	8541.0
11	7598.5
12	7872.0
13	9915.5
14	14789.5
15	21429.0
16	26913.5
17	30558.0
18	32198.5
19	31752.0
20	30387.5
21	29452.5
22	29866.5
23	33005.5
24	39135.0
25	48047.0
26	59789.5
27	73187.5
28	87512.0
29	103011.0
30	117277.5
31	129650.0
32	142846.5
33	156684.0
34	169978.5
35	182488.5
36	196712.5
37	210966.5
38	220677.0
39	228435.0
40	237227.0
41	245301.5
42	251111.5
43	256998.5
44	264389.5
45	269827.0
46	272055.5
47	273958.0
48	274809.0
49	273217.0
50	269908.5
51	264775.5
52	256154.5
53	244148.0
54	231246.5
55	217351.5
56	201358.0
57	183914.5
58	165389.5
59	144080.5
60	121358.5
61	99438.5
62	79487.0
63	61757.0
64	46655.5
65	35749.5
66	27550.5
67	21135.0
68	16090.5
69	11874.0
70	8663.0
71	6181.0
72	4361.5
73	3004.0
74	1926.0
75	1267.5
76	878.5
77	570.0
78	358.5
79	219.0
80	144.0
81	94.5
82	60.0
83	36.0
84	24.0
85	19.5
86	15.5
87	12.0
88	8.0
89	6.0
90	5.5
91	5.0
92	4.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.962804957266976E-4
20-21	0.0
22-23	5.672936115900727E-4
24-25	0.006389164971115456
26-27	0.028039205745564504
28-29	0.027203865189907646
30-31	0.02848997741302119
32-33	0.049663407661909784
34-35	0.02262448056720405
36-37	0.01907115348653853
38-39	0.014246349402827912
40-41	0.007634493251268692
42-43	0.011058867495700389
44-45	0.004708308014557366
46-47	0.003511766784813128
48-49	0.008384602911759196
50-51	0.0020134149505489996
52-53	0.0020679649997352835
54-55	0.0011006830301543817
56-57	0.006982217946647971
58-59	0.004536577931674883
60-61	0.007931114551014229
62-63	0.006261640330473247
64-65	0.0014111723800210247
66-67	0.004701739105597536
68-69	0.011728160948303975
70-71	0.01513084039978134
72-73	0.00534418990265871
74-75	0.0014523589233127194
76-77	0.002526721311621033
78-79	0.0030298612323555584
80-81	0.003690700256635479
82-83	0.0034129419168685477
84-85	0.004334309907534375
86-87	1.0565516078443885E-5
88-89	2.466758816196009E-4
90-91	0.003989947077603599
92-93	6.989202458779237E-4
94-95	0.0022955234240072623
96-97	5.571457423618801E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	149.0
20-21	439.0
22-23	1015.0
24-25	2225.0
26-27	3787.0
28-29	8373.0
30-31	18431.0
32-33	23853.0
34-35	33292.0
36-37	50441.0
38-39	56850.0
40-41	57598.0
42-43	65175.0
44-45	71918.0
46-47	72679.0
48-49	67576.0
50-51	60054.0
52-53	56947.0
54-55	60636.0
56-57	64259.0
58-59	63707.0
60-61	62524.0
62-63	60906.0
64-65	63823.0
66-67	66623.0
68-69	67274.0
70-71	65909.0
72-73	63169.0
74-75	63645.0
76-77	64203.0
78-79	68744.0
80-81	68331.0
82-83	65072.0
84-85	65697.0
86-87	69550.0
88-89	73558.0
90-91	79132.0
92-93	82744.0
94-95	98896.0
96-97	229039.0
98-99	1338194.0
100-101	2778399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.76257595841612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.65734950967344	78.65665173021719
2	6.672640138089498	11.578708936483327
3	1.5740397324062134	4.097032255333772
4	0.5223665612081649	1.8128747377984016
5	0.23126089696396912	1.0032395569523909
6	0.11413800566542161	0.5941744431772964
7	0.06848257142794358	0.41592070137412274
8	0.04324778787616933	0.3001831584511687
9	0.029822865854605217	0.2328757797607056
>10	0.08445104474888507	1.1282056618985756
>50	0.001784802039189629	0.10073486838866196
>100	3.8954157843709307E-4	0.06235676270533385
>500	2.6542468045049916E-5	0.017041407459130326
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5540411597125398E-5	0.0	0.0	0.0
3	0.0	1.5540411597125398E-5	0.0	0.0	0.0
4	0.0	1.5540411597125398E-5	0.0	1.5540411597125398E-5	0.0
5	0.0	1.5540411597125398E-5	0.0	1.5540411597125398E-5	0.0
6	0.0	1.5540411597125398E-5	0.0	3.1080823194250796E-5	0.0
7	0.0	1.5540411597125398E-5	0.0	3.1080823194250796E-5	0.0
8	0.0	1.5540411597125398E-5	0.0	3.1080823194250796E-5	1.5540411597125398E-5
9	0.0	1.0878288117987778E-4	0.0	3.1080823194250796E-5	1.5540411597125398E-5
10-11	0.0	1.0878288117987778E-4	0.0	4.662123479137619E-5	1.5540411597125398E-5
12-13	0.0	1.0878288117987778E-4	0.0	4.662123479137619E-5	1.5540411597125398E-5
14-15	0.0	1.9425514496406745E-4	0.0	5.439144058993889E-5	2.3310617395688097E-5
16-17	0.0	2.0202535076263017E-4	0.0	6.216164638850159E-5	3.1080823194250796E-5
18-19	0.0	2.0979555656119286E-4	0.0	7.770205798562698E-5	3.1080823194250796E-5
20-21	0.0	2.3310617395688095E-4	0.0	1.1655308697844049E-4	4.662123479137619E-5
22-23	0.0	2.3310617395688095E-4	0.0	1.6317432176981666E-4	6.216164638850159E-5
24-25	0.0	2.4864658555400637E-4	0.0	2.6418699715113176E-4	9.324246958275237E-5
26-27	0.0	2.6418699715113176E-4	0.0	4.4290173051807387E-4	9.324246958275237E-5
28-29	0.0	2.7195720294969446E-4	0.0	0.0012976243683599708	9.324246958275237E-5
30-31	0.0	3.263486435396333E-4	0.0	0.004164830308029607	1.0101267538131507E-4
32-33	0.0	3.263486435396333E-4	0.0	0.009774918894591875	1.2432329277700318E-4
34-35	0.0	3.962804957266976E-4	0.0	0.017459652429370386	1.3986370437412858E-4
36-37	0.0	4.662123479137619E-4	0.0	0.029184892979401494	1.3986370437412858E-4
38-39	0.0	4.662123479137619E-4	0.0	0.05916234695025639	1.3986370437412858E-4
40-41	0.0	4.972931711080127E-4	0.0	0.09077931434460801	1.3986370437412858E-4
42-43	0.0	5.128335827051381E-4	0.0	0.11045347542656876	1.4763391017269127E-4
44-45	0.0	6.138462580864532E-4	0.0	0.1346887473122858	1.5540411597125397E-4
46-47	0.0	6.993185218706428E-4	0.0	0.16111521723319755	1.5540411597125397E-4
48-49	0.0	7.070887276692055E-4	0.0	0.18651601998869902	1.7094452756837936E-4
50-51	0.0	8.00331197251958E-4	0.0	0.21169148677604216	1.7094452756837936E-4
52-53	0.0	8.081014030505207E-4	0.0	0.2661295486007724	1.7871473336694205E-4
54-55	0.0	8.081014030505207E-4	0.0	0.33051347384766294	1.8648493916550475E-4
56-57	0.0	8.081014030505207E-4	0.0	0.39241093323901344	1.8648493916550475E-4
58-59	0.0	8.391822262447715E-4	0.0	0.4364369192936697	1.8648493916550475E-4
60-61	0.0	8.858034610361476E-4	0.0	0.4675488233111147	1.8648493916550475E-4
62-63	0.0	9.324246958275238E-4	0.0	0.49298847709560895	1.8648493916550475E-4
64-65	0.0	0.0011189096349930286	0.0	0.5051643895819566	1.8648493916550475E-4
66-67	0.0	0.0011189096349930286	0.0	0.5155920057636278	1.8648493916550475E-4
68-69	0.0	0.0011733010755829675	0.0	0.5260584729742919	1.8648493916550475E-4
70-71	0.0	0.0012043818987772183	0.0	0.5336655044510847	1.8648493916550475E-4
72-73	0.0	0.001212152104575781	0.0	0.5392833632434455	2.0202535076263017E-4
74-75	0.0	0.0012510031335685944	0.0	0.5439144058993889	2.0202535076263017E-4
76-77	0.0	0.0013287051915542214	0.0	0.5480559255900228	2.0202535076263017E-4
78-79	0.0	0.0013675562205470349	0.0	0.5507988082369154	2.0202535076263017E-4
80-81	0.0	0.0013675562205470349	0.0	0.5516224500515631	2.0202535076263017E-4
82-83	0.0	0.0013675562205470349	0.0	0.5522906877502395	2.0202535076263017E-4
84-85	0.0	0.0013675562205470349	0.0	0.5525160237183978	2.0202535076263017E-4
86-87	0.0	0.0013675562205470349	0.0	0.5525937257763834	2.0202535076263017E-4
88	0.0	0.0013986370437412857	0.0	0.552601495982182	2.0202535076263017E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7245	0.0	44.122864	1
GTATCAA	12330	0.0	31.839788	1
TCAACGC	14690	0.0	25.91138	4
ATCAACG	14920	0.0	25.53955	3
CAACGCA	15015	0.0	25.32309	5
AACGCAG	15410	0.0	24.700724	6
TATCAAC	15620	0.0	24.579622	2
ACGCAGA	17985	0.0	21.095486	7
GTACATG	17870	0.0	20.8163	1
CGCAGAG	18345	0.0	20.659056	8
TACATGG	17510	0.0	20.538488	2
ACATGGG	18005	0.0	19.516245	3
GTGGTAT	4175	0.0	18.451237	1
CATGGGG	13730	0.0	18.152029	4
GCAGAGT	21885	0.0	17.260887	9
AGAGTAC	19425	0.0	16.997414	10-11
GAGTACT	12400	0.0	16.959553	12-13
AGGACCG	1100	0.0	16.477842	5
CAGAGTA	21055	0.0	16.336973	10-11
TGGTATC	4010	0.0	16.33402	2
>>END_MODULE