FastQCFastQC Report
Fri 27 May 2016
SRR2140223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4765099
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.047.5058751
GTATCAA93300.034.740411
TCAACGC109400.028.921484
ATCAACG110700.028.655323
CAACGCA111100.028.478945
AACGCAG115500.027.4292376
TATCAAC116450.027.415012
ACGCAGA136350.023.2647157
CGCAGAG139350.022.7054888
GTACATG139550.020.0501161
TACATGG136800.019.8883272
ACATGGG138600.019.1605593
GCAGAGT165850.019.0039759
GAGTACT91150.018.02531812-13
CATGGGG101500.017.4293614
CAGAGTA161650.017.08253510-11
AGAGTAC149000.016.97705510-11
GTGGTAT26150.016.6406521
GTATAGG14900.015.5577521
GTACTTT105850.015.42639114-15
Produced by FastQC (version 0.11.5)