##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4765099
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.369436815478544	33.0	31.0	34.0	31.0	34.0
2	32.65732674179487	34.0	31.0	34.0	31.0	34.0
3	32.3291125745761	33.0	31.0	34.0	30.0	34.0
4	36.18991777505567	37.0	35.0	37.0	35.0	37.0
5	36.07872260366469	37.0	35.0	37.0	35.0	37.0
6	36.166654249995645	37.0	36.0	37.0	35.0	37.0
7	36.14634050625181	37.0	36.0	37.0	35.0	37.0
8	36.150668852840205	37.0	37.0	37.0	35.0	37.0
9	37.97582736476199	39.0	38.0	39.0	35.0	39.0
10-11	37.90393074729403	39.0	38.0	39.0	35.0	39.0
12-13	37.872157640376415	39.0	38.0	39.0	35.0	39.0
14-15	39.26289674149477	41.0	39.0	41.0	36.0	41.0
16-17	39.25534506208581	41.0	39.0	41.0	36.0	41.0
18-19	39.235227641650255	41.0	39.0	41.0	36.0	41.0
20-21	39.22825136826118	40.5	39.0	41.0	36.0	41.0
22-23	39.16751760290899	40.0	39.0	41.0	36.0	41.0
24-25	39.11664038104479	40.0	39.0	41.0	36.0	41.0
26-27	38.96680029718978	40.0	38.5	41.0	35.0	41.0
28-29	38.855139312635536	40.0	38.0	41.0	35.0	41.0
30-31	38.727800223031664	40.0	38.0	41.0	35.0	41.0
32-33	38.57545836796912	40.0	38.0	41.0	34.5	41.0
34-35	38.65553623010237	40.0	38.0	41.0	35.0	41.0
36-37	38.646463023866346	40.0	38.0	41.0	35.0	41.0
38-39	38.60960800079247	40.0	38.0	41.0	35.0	41.0
40-41	38.54257728778178	40.0	38.0	41.0	34.0	41.0
42-43	38.51476465185229	40.0	38.0	41.0	34.0	41.0
44-45	38.56264198887321	40.0	38.0	41.0	34.0	41.0
46-47	38.520727449273615	40.0	38.0	41.0	34.0	41.0
48-49	38.44225292079735	40.0	38.0	41.0	34.0	41.0
50-51	38.35339766352401	40.0	38.0	41.0	34.0	41.0
52-53	38.15791491419916	40.0	37.0	41.0	33.0	41.0
54-55	38.05494441064194	40.0	37.0	41.0	33.0	41.0
56-57	37.917370598280186	40.0	37.0	41.0	33.0	41.0
58-59	37.735137829260026	39.5	36.0	41.0	33.0	41.0
60-61	37.542329792608456	39.0	36.0	41.0	33.0	41.0
62-63	37.2628709535468	39.0	35.0	41.0	32.0	41.0
64-65	36.95004133123837	38.5	35.0	40.0	32.0	41.0
66-67	36.66943312607855	38.0	35.0	40.0	31.5	41.0
68-69	36.37832848400828	37.0	35.0	40.0	31.0	41.0
70-71	36.07279359905192	37.0	35.0	39.0	31.0	41.0
72-73	35.735330412197285	36.0	34.5	39.0	31.0	41.0
74-75	35.38931615750567	36.0	34.0	39.0	31.0	40.0
76-77	34.1890764755114	35.0	32.5	37.0	29.5	39.0
78-79	34.780583023173335	35.0	34.0	37.0	30.5	39.0
80-81	34.640652621644094	35.0	34.0	37.0	31.0	39.0
82-83	34.41117982054688	35.0	34.0	36.0	31.0	38.5
84-85	34.21235884252927	35.0	34.0	36.0	30.5	37.0
86-87	33.99497073047041	35.0	34.0	35.5	30.0	37.0
88-89	33.83778862794158	35.0	34.0	35.0	30.0	36.5
90-91	33.67966073310997	35.0	34.0	35.0	30.0	36.0
92-93	33.57203238056367	35.0	34.0	35.0	30.0	36.0
94-95	33.542621815464585	35.0	34.0	35.0	30.0	36.0
96-97	33.556114636681656	35.0	34.0	35.0	30.0	35.0
98-99	33.61115221509064	35.0	34.0	35.0	30.0	35.0
100	32.53989659700074	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	22.0
21	101.0
22	447.0
23	1648.0
24	4339.0
25	9459.0
26	16971.0
27	27498.0
28	39611.0
29	53631.0
30	69714.0
31	88246.0
32	111139.0
33	144964.0
34	202131.0
35	293625.0
36	492555.0
37	959613.0
38	1347653.0
39	859897.0
40	41828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25937383462547	20.040129281679143	13.586769131134528	24.11372775256086
2	15.22776336860997	21.153012770563635	40.337357104228055	23.28186675659834
3	18.15139622492628	25.854027376975797	30.557980012587354	25.436596385510562
4	12.67598007932259	17.555354044060785	38.59378367584807	31.174882200768543
5	13.268328737765994	37.82609763196945	35.61909206923088	13.286481561033675
6	30.583351993316406	37.54364809629349	18.507611279429874	13.365388630960236
7	26.878224355884317	32.48089913766744	23.110243879508065	17.530632626940175
8	24.91211620157315	36.12090745648726	21.235193644455236	17.731782697484356
9	25.75650579347879	16.328349106702714	21.212990538076966	36.70215456174153
10-11	24.40354334715816	26.48184224504045	28.950300927640747	20.164313480160644
12-13	25.061924211857928	24.115312189736247	28.96873706086694	21.854026537538886
14-15	22.191490669973486	25.583403408827394	26.527350218746765	25.69775570245235
16-17	21.886942957533517	28.619310952406234	27.283651399477744	22.210094690582505
18-19	21.925036896042087	27.94611420275956	28.80201843516711	21.326830466031247
20-21	22.695371692816472	27.16479996272783	28.647292064690298	21.4925362797654
22-23	22.45666796864424	27.00628202429318	28.450952420030696	22.086097587031883
24-25	22.198706402488945	27.117893296049512	28.59235723612668	22.091043065334862
26-27	22.1268535471679	27.338903102938307	28.467777049863486	22.06646630003031
28-29	22.100168761891272	27.39945606574537	28.487198484305754	22.013176688057605
30-31	22.39878713956096	27.242312162071542	28.365151338575433	21.993749359792066
32-33	22.172123976833586	27.38432429599727	28.300715413509685	22.142836313659462
34-35	22.288501688624944	27.42845149463575	28.356526725349408	21.9265200913899
36-37	22.262394769101533	27.32065427665868	28.30287874339856	22.114072210841226
38-39	22.092739564708378	27.514444488507344	28.347804403262877	22.045011543521404
40-41	22.375143689215367	27.347337384290938	28.203351295550828	22.074167630942867
42-43	22.239434943721147	27.449570306391085	28.02586409975939	22.285130650128377
44-45	22.317154240884225	27.5315373008388	27.90947166121334	22.241836797063634
46-47	22.323150773419837	27.49250549927752	27.749999887148007	22.434343840154632
48-49	22.31517170438451	27.55332330421229	27.748039203914214	22.383465787488984
50-51	22.284987422265992	27.71518620741385	27.611332508245095	22.388493862075066
52-53	22.46553412926722	27.584763283813057	27.452333911153758	22.497368675765962
54-55	22.464962321290233	27.45225873996334	27.542003490599704	22.54077544814672
56-57	22.4216470239728	27.552594521663888	27.54806477253649	22.47769368182683
58-59	22.34263447169429	27.58182133707757	27.54731978918013	22.528224402048007
60-61	22.392913872084698	27.60652695549049	27.545644753457193	22.45491441896762
62-63	22.444323174802726	27.634323754607642	27.484943113174186	22.436409957415442
64-65	22.378631204051793	27.606494003146327	27.45429356074567	22.56058123205622
66-67	22.42543631881886	27.679428389291626	27.280933555876214	22.6142017360133
68-69	22.416241771110577	27.63572266062555	27.38673911237935	22.561296455884523
70-71	22.363189026442733	27.413073671445993	27.452819983772887	22.770917318338388
72-73	22.41495025687841	27.42629821884308	27.36821904298632	22.79053248129219
74-75	22.354874094901287	27.650314691806336	27.297058309260873	22.6977529040315
76-77	22.500282767626707	27.537385307736866	27.233134196374404	22.729197728262022
78-79	22.453660178866706	27.50210260723297	27.23574684205304	22.80849037184729
80-81	22.446285786093608	27.498932422975074	27.212280393999176	22.842501396932136
82-83	22.60281395145551	27.48736207875809	27.324693562974446	22.585130406811952
84-85	22.650452207755563	27.463284717744706	27.243506035460737	22.64275703903899
86-87	22.467144447149458	27.457065802325875	27.343070198664517	22.73271955186015
88-89	22.555569830716838	27.460463367718734	27.33409555665774	22.64987124490669
90-91	22.626249700611943	27.40412255631449	27.36923166858996	22.600396074483605
92-93	22.735899729182954	27.342604992831497	27.388637946983422	22.532857331002123
94-95	22.51511976000077	27.391525905668036	27.466238127034735	22.627116207296464
96-97	22.589131907607953	27.46276377845236	27.600807616356626	22.34729669758306
98-99	23.633832969897515	29.406265845792767	27.774698931061593	19.185202253248125
100	30.388662541198354	40.804804306546465	28.80653315225518	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	913.0
1	827.5
2	1200.0
3	1765.5
4	2296.0
5	3598.5
6	4833.0
7	5874.5
8	6684.5
9	6691.0
10	6029.5
11	5339.0
12	5608.0
13	7191.5
14	10914.0
15	16109.5
16	20367.0
17	22823.5
18	23539.5
19	23017.5
20	22022.5
21	21215.0
22	21397.0
23	23305.5
24	27652.5
25	34547.5
26	43771.5
27	53932.0
28	64001.5
29	75204.5
30	85491.5
31	94159.0
32	104319.0
33	115092.0
34	125358.0
35	135756.0
36	146859.0
37	156661.5
38	164721.5
39	171897.5
40	179332.0
41	185861.0
42	189981.0
43	195315.0
44	200830.0
45	204664.5
46	207011.5
47	208114.0
48	209311.5
49	209406.5
50	207217.5
51	203109.5
52	195816.5
53	186789.5
54	176822.5
55	165854.0
56	153150.0
57	138693.0
58	123913.0
59	107767.5
60	91632.0
61	75853.0
62	61256.0
63	48452.5
64	36978.5
65	27901.5
66	21027.0
67	16143.5
68	12392.5
69	9242.0
70	6788.5
71	4773.0
72	3385.5
73	2398.0
74	1625.5
75	1059.5
76	669.5
77	434.5
78	299.0
79	207.5
80	146.5
81	105.0
82	75.0
83	51.0
84	32.5
85	22.0
86	18.5
87	15.0
88	10.5
89	6.0
90	4.5
91	3.0
92	2.5
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.5119734133540566E-4
20-21	0.0
22-23	6.401407050257866E-4
24-25	0.006392003634940392
26-27	0.027885878721205678
28-29	0.026051955372580253
30-31	0.027963935048735337
32-33	0.04824008184762048
34-35	0.023285589211866083
36-37	0.018791752257225774
38-39	0.014136444270516376
40-41	0.007519210985578131
42-43	0.010314837883298495
44-45	0.004753735356101536
46-47	0.0035659957478886145
48-49	0.008050094457109748
50-51	0.001979339171573634
52-53	0.0018023646321754156
54-55	9.46915334524474E-4
56-57	0.0067342528424358585
58-59	0.00430795703935349
60-61	0.007876951450206976
62-63	0.006258017696380141
64-65	0.0012222255542918893
66-67	0.00447109786530626
68-69	0.012035693020127204
70-71	0.01575671940633833
72-73	0.005117315797287129
74-75	0.001607957525058802
76-77	0.0024540200275453465
78-79	0.0029922027371835605
80-81	0.0037457439867380872
82-83	0.0035595478627857453
84-85	0.004182615621267799
86-87	1.4808468015115892E-5
88-89	2.861718163536047E-4
90-91	0.00401980875836546
92-93	4.5360163609417704E-4
94-95	0.001948837582278485
96-97	2.625960773397967E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	312.0
22-23	600.0
24-25	1453.0
26-27	1654.0
28-29	6611.0
30-31	15269.0
32-33	19805.0
34-35	27035.0
36-37	40596.0
38-39	45300.0
40-41	46495.0
42-43	53997.0
44-45	59993.0
46-47	59176.0
48-49	54568.0
50-51	48554.0
52-53	46881.0
54-55	50665.0
56-57	52886.0
58-59	51322.0
60-61	50490.0
62-63	50099.0
64-65	53395.0
66-67	54870.0
68-69	54590.0
70-71	53613.0
72-73	51326.0
74-75	53228.0
76-77	53624.0
78-79	55635.0
80-81	54112.0
82-83	52904.0
84-85	53577.0
86-87	56203.0
88-89	59284.0
90-91	62500.0
92-93	65166.0
94-95	73288.0
96-97	165676.0
98-99	955511.0
100-101	1952748.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.85072113887175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.98889166448427	81.73279361154975
2	5.981592411654939	10.629375986686886
3	1.209878931108654	3.224958465591938
4	0.38956113489723865	1.3845115105318777
5	0.17104755864854856	0.7598849467483497
6	0.09155396412193864	0.4880781441213984
7	0.05209728166378427	0.32402167316415065
8	0.03464949198165286	0.24629058797323292
9	0.019947870014789485	0.15951443723986697
>10	0.05849917608916021	0.8040157972286432
>50	0.001513561027523062	0.08864198876762346
>100	7.669543075341169E-4	0.1579128503962584
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0985922852809565E-5
2	0.0	4.197184570561913E-5	0.0	0.0	2.0985922852809565E-5
3	0.0	4.197184570561913E-5	0.0	0.0	2.0985922852809565E-5
4	0.0	6.295776855842869E-5	0.0	0.0	2.0985922852809565E-5
5	0.0	1.4690145996966694E-4	0.0	0.0	2.0985922852809565E-5
6	0.0	1.4690145996966694E-4	0.0	0.0	4.197184570561913E-5
7	0.0	1.4690145996966694E-4	0.0	0.0	4.197184570561913E-5
8	0.0	1.4690145996966694E-4	0.0	0.0	4.197184570561913E-5
9	0.0	2.308451513809052E-4	0.0	0.0	6.295776855842869E-5
10-11	2.0985922852809565E-5	2.308451513809052E-4	0.0	3.1478884279214346E-5	8.394369141123826E-5
12-13	2.0985922852809565E-5	2.4133811280731E-4	0.0	4.197184570561913E-5	1.4690145996966694E-4
14-15	2.0985922852809565E-5	3.462677270713578E-4	0.0	4.197184570561913E-5	1.4690145996966694E-4
16-17	2.0985922852809565E-5	4.092254956297865E-4	0.0	4.197184570561913E-5	1.5739442139607173E-4
18-19	2.0985922852809565E-5	4.931691870410247E-4	0.0	6.295776855842869E-5	1.9936626710169084E-4
20-21	2.0985922852809565E-5	5.456339941730487E-4	0.0	3.1478884279214346E-4	2.0985922852809563E-4
22-23	4.197184570561913E-5	5.876058398786679E-4	0.0	0.0011961976026101451	2.3084515138090518E-4
24-25	4.197184570561913E-5	6.295776855842869E-4	0.0	0.003021972890804577	2.728169970865243E-4
26-27	4.197184570561913E-5	6.295776855842869E-4	0.0	0.007324087075630538	2.938029199393339E-4
28-29	4.197184570561913E-5	6.295776855842869E-4	0.0	0.028467404349836176	2.938029199393339E-4
30-31	4.197184570561913E-5	6.715495312899061E-4	0.0	0.031940574581976154	2.938029199393339E-4
32-33	4.197184570561913E-5	6.715495312899061E-4	0.0	0.03922268981190108	2.938029199393339E-4
34-35	4.197184570561913E-5	7.659861841275491E-4	0.0	0.04816269294719795	2.938029199393339E-4
36-37	4.197184570561913E-5	8.499298755387873E-4	0.0	0.06281086709845903	2.938029199393339E-4
38-39	4.197184570561913E-5	8.814087598180017E-4	0.0	0.09543348417315149	2.938029199393339E-4
40-41	4.197184570561913E-5	9.863383740820494E-4	0.0	0.13000779207315524	2.938029199393339E-4
42-43	4.197184570561913E-5	0.001059789104066883	0.0	0.15481315288517616	3.0429588136573864E-4
44-45	4.197184570561913E-5	0.0011857046411837403	0.0	0.18294478246936735	3.1478884279214346E-4
46-47	4.197184570561913E-5	0.0012591553711685738	0.0	0.21408789198293676	3.3577476564495304E-4
48-49	4.197184570561913E-5	0.0012696483325949786	0.0	0.2430799444040932	3.5676068849776257E-4
50-51	4.197184570561913E-5	0.0014165497925646454	0.0	0.2699104467714102	3.5676068849776257E-4
52-53	4.197184570561913E-5	0.0014270427539910503	0.0	0.3314411725758478	3.7774661135057215E-4
54-55	6.295776855842869E-5	0.0015319723682550982	0.0	0.40600415647188026	3.9873253420338173E-4
56-57	6.295776855842869E-5	0.0015739442139607173	0.0	0.47777601262848896	3.9873253420338173E-4
58-59	6.295776855842869E-5	0.001584437175387122	0.0	0.5270509594868857	3.9873253420338173E-4
60-61	9.443665283764304E-5	0.0016264090210927412	0.0	0.5617301970011537	3.9873253420338173E-4
62-63	1.0492961426404781E-4	0.001636901982519146	0.0	0.5901451365438577	3.9873253420338173E-4
64-65	1.0492961426404781E-4	0.0017208456739303843	0.0	0.6052340150750278	3.9873253420338173E-4
66-67	1.2591553711685738E-4	0.0017628175196360034	0.0	0.61903225935075	3.9873253420338173E-4
68-69	1.2591553711685738E-4	0.0018047893653416226	0.0	0.630826347994029	3.9873253420338173E-4
70-71	1.2591553711685738E-4	0.0018467612110472415	0.0	0.6388639564466552	3.9873253420338173E-4
72-73	1.2591553711685738E-4	0.001867747133900051	0.0	0.6447714937297211	4.4070437990900084E-4
74-75	1.2591553711685738E-4	0.0018887330567528607	0.0	0.6501543829414667	4.4070437990900084E-4
76-77	1.2591553711685738E-4	0.0018887330567528607	0.0	0.6548972015062017	4.4070437990900084E-4
78-79	1.2591553711685738E-4	0.001920211941032075	0.0	0.6583388928540624	4.4070437990900084E-4
80-81	1.2591553711685738E-4	0.0019307049024584798	0.0	0.6594091749195556	4.4070437990900084E-4
82-83	1.2591553711685738E-4	0.0019307049024584798	0.0	0.6602171329493889	4.721832641882152E-4
84-85	1.2591553711685738E-4	0.0019516908253112894	0.0	0.6605214288307546	4.8267622561461995E-4
86-87	1.2591553711685738E-4	0.0019516908253112894	0.0	0.6606053725221659	4.8267622561461995E-4
88	1.2591553711685738E-4	0.002014648593869718	0.0	0.6606158654835923	4.8267622561461995E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5145	0.0	47.505875	1
GTATCAA	9330	0.0	34.74041	1
TCAACGC	10940	0.0	28.92148	4
ATCAACG	11070	0.0	28.65532	3
CAACGCA	11110	0.0	28.47894	5
AACGCAG	11550	0.0	27.429237	6
TATCAAC	11645	0.0	27.41501	2
ACGCAGA	13635	0.0	23.264715	7
CGCAGAG	13935	0.0	22.705488	8
GTACATG	13955	0.0	20.050116	1
TACATGG	13680	0.0	19.888327	2
ACATGGG	13860	0.0	19.160559	3
GCAGAGT	16585	0.0	19.003975	9
GAGTACT	9115	0.0	18.025318	12-13
CATGGGG	10150	0.0	17.429361	4
CAGAGTA	16165	0.0	17.082535	10-11
AGAGTAC	14900	0.0	16.977055	10-11
GTGGTAT	2615	0.0	16.640652	1
GTATAGG	1490	0.0	15.557752	1
GTACTTT	10585	0.0	15.426391	14-15
>>END_MODULE