FastQCFastQC Report
Fri 27 May 2016
SRR2140222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6312239
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68750.046.440951
GTATCAA126000.035.8898351
TCAACGC145600.030.2905834
ATCAACG146300.030.1173443
CAACGCA149750.029.423495
TATCAAC155100.028.7556572
AACGCAG153500.028.6776966
ACGCAGA179250.024.5349277
CGCAGAG191950.022.9331938
GTACATG183000.022.402861
TACATGG185600.021.687412
ACATGGG186950.021.2649883
GTGGTAT38000.019.9428331
GCAGAGT220250.019.8548859
CATGGGG142200.018.812754
TGGTATC38000.018.0902212
GAGTACT122250.018.00419812-13
CAGAGTA211750.017.53259810-11
AGAGTAC195650.017.51489410-11
ACCGAAC8550.016.467678
Produced by FastQC (version 0.11.5)