FastQCFastQC Report
Fri 27 May 2016
SRR2140221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8114444
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.046.1048771
GTATCAA161100.035.568711
ATCAACG194100.028.8393973
TCAACGC194100.028.818084
CAACGCA198150.028.2499455
TATCAAC205600.027.648882
AACGCAG203850.027.4397326
ACGCAGA234150.023.8359077
CGCAGAG240500.023.2237638
GTACATG240650.021.7995381
TACATGG239250.021.6677112
ACATGGG242400.020.6863543
GCAGAGT283050.019.6010599
CATGGGG189950.019.0582354
GTGGTAT49050.018.725261
GAGTACT155700.018.0956312-13
AGAGTAC254450.017.52791410-11
CAGAGTA276650.017.46731210-11
TGGTATC49100.016.7681642
GTACTTT172600.016.3841414-15
Produced by FastQC (version 0.11.5)