##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8114444
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34641868253697	33.0	31.0	34.0	31.0	34.0
2	32.63548026211038	34.0	31.0	34.0	31.0	34.0
3	32.304914298502766	33.0	31.0	34.0	30.0	34.0
4	36.17806333989119	37.0	35.0	37.0	35.0	37.0
5	36.06106086874221	37.0	35.0	37.0	35.0	37.0
6	36.15003307681956	37.0	36.0	37.0	35.0	37.0
7	36.12892737937436	37.0	36.0	37.0	35.0	37.0
8	36.12934268817432	37.0	36.0	37.0	35.0	37.0
9	37.9487865095871	39.0	38.0	39.0	35.0	39.0
10-11	37.87323222638544	39.0	38.0	39.0	35.0	39.0
12-13	37.83909378141004	39.0	38.0	39.0	35.0	39.0
14-15	39.22022223580568	41.0	39.0	41.0	36.0	41.0
16-17	39.20800297592786	41.0	39.0	41.0	36.0	41.0
18-19	39.17986438750455	40.5	39.0	41.0	36.0	41.0
20-21	39.172593168673885	40.0	39.0	41.0	36.0	41.0
22-23	39.11186922428916	40.0	39.0	41.0	36.0	41.0
24-25	39.05868595820182	40.0	39.0	41.0	35.0	41.0
26-27	38.901224630208844	40.0	38.0	41.0	35.0	41.0
28-29	38.78204942535962	40.0	38.0	41.0	35.0	41.0
30-31	38.65258054879415	40.0	38.0	41.0	35.0	41.0
32-33	38.496655081762015	40.0	38.0	41.0	34.0	41.0
34-35	38.58245295627951	40.0	38.0	41.0	34.0	41.0
36-37	38.572492969827024	40.0	38.0	41.0	34.0	41.0
38-39	38.52554025273727	40.0	38.0	41.0	34.0	41.0
40-41	38.45732791789934	40.0	38.0	41.0	34.0	41.0
42-43	38.42856885043228	40.0	38.0	41.0	34.0	41.0
44-45	38.47580910128163	40.0	38.0	41.0	34.0	41.0
46-47	38.42925138382195	40.0	38.0	41.0	34.0	41.0
48-49	38.34548428307876	40.0	38.0	41.0	34.0	41.0
50-51	38.24964804040417	40.0	37.5	41.0	33.5	41.0
52-53	38.046754676454825	40.0	37.0	41.0	33.0	41.0
54-55	37.94092873272509	40.0	37.0	41.0	33.0	41.0
56-57	37.79677394948038	40.0	36.5	41.0	33.0	41.0
58-59	37.60864415308099	39.0	36.0	41.0	33.0	41.0
60-61	37.41385548919909	39.0	35.5	41.0	32.5	41.0
62-63	37.132683663162425	39.0	35.0	40.5	32.0	41.0
64-65	36.81708496970044	38.0	35.0	40.0	31.5	41.0
66-67	36.53556960787889	37.5	35.0	40.0	31.0	41.0
68-69	36.2428481066458	37.0	35.0	40.0	31.0	41.0
70-71	35.942724041557966	36.5	35.0	39.0	31.0	41.0
72-73	35.60515030779136	36.0	34.0	39.0	31.0	40.5
74-75	35.258268898444214	35.5	34.0	38.5	30.0	40.0
76-77	34.07933816182982	35.0	32.5	37.0	29.5	39.0
78-79	34.679192484168794	35.0	34.0	37.0	30.0	39.0
80-81	34.54385970305161	35.0	34.0	37.0	30.0	39.0
82-83	34.315815088437446	35.0	34.0	36.0	30.0	38.0
84-85	34.1211349001166	35.0	34.0	36.0	30.0	37.0
86-87	33.90355274458524	35.0	34.0	35.5	30.0	37.0
88-89	33.74584490705699	35.0	34.0	35.0	30.0	36.5
90-91	33.58810986067131	35.0	34.0	35.0	30.0	36.0
92-93	33.48237077379133	35.0	33.5	35.0	30.0	36.0
94-95	33.461236202342974	35.0	33.0	35.0	30.0	36.0
96-97	33.47940018662939	35.0	33.0	35.0	30.0	35.0
98-99	33.54166596616228	35.0	33.0	35.0	30.0	35.0
100	32.43332444235	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	36.0
21	164.0
22	883.0
23	3050.0
24	8058.0
25	17441.0
26	31698.0
27	50749.0
28	73281.0
29	99127.0
30	128010.0
31	161000.0
32	203213.0
33	262900.0
34	365984.0
35	529487.0
36	878437.0
37	1669497.0
38	2250656.0
39	1319191.0
40	61577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.954310116626594	20.054152816878148	13.926068132332912	24.06546893416234
2	15.18354184217674	20.858385368116412	40.66101140139731	23.297061388309537
3	18.322795745463274	25.554381791284776	30.35144490491277	25.771377558339182
4	12.815197196505393	17.549508013118338	38.272739327549736	31.362555462826535
5	13.43420448770119	37.68317336344918	35.44813421597339	13.434487932876237
6	30.865663747263522	37.26040872301294	18.378486560508644	13.495440969214897
7	27.305592348656297	32.34413842772222	22.65301233208338	17.697256891538103
8	25.007418869364308	36.31168075101633	20.840072345067632	17.84082803455172
9	25.743698520810543	16.621656394449207	21.123246398644195	36.51139868609605
10-11	24.690163614414	26.447117017506066	28.54279356663254	20.319925801447393
12-13	25.292823513231465	23.963878486314037	28.698559014024866	22.044738986429632
14-15	22.362382438032725	25.466982087743784	26.26788723910104	25.90274823512246
16-17	22.089511000384007	28.500510940737282	27.014395564255544	22.395582494623167
18-19	22.113154782822164	27.805104716018075	28.5608169231521	21.52092357800766
20-21	22.907138971085594	26.978736651366898	28.41164907306949	21.70247530447802
22-23	22.6660266610924	26.89172735072326	28.241427127797706	22.200818860386633
24-25	22.38719964967296	27.04613872191287	28.385261853927336	22.18139977448683
26-27	22.347961783046625	27.199417740413377	28.27663498368563	22.175985492854366
28-29	22.28573763428171	27.30772703821156	28.24118036742762	22.16535496007911
30-31	22.59144764685351	27.164698555633297	28.135038352293627	22.10881544521957
32-33	22.357504718226647	27.288645336573865	28.121114524696573	22.23273542050292
34-35	22.47227516692597	27.30736331557864	28.137510267448157	22.082851250047224
36-37	22.47915697676784	27.208303297385335	28.05890507100689	22.253634654839935
38-39	22.293151355239733	27.339703475103416	28.16863492329471	22.198510246362144
40-41	22.548723093797385	27.178278426823717	28.049856416894404	22.223142062484502
42-43	22.44992586068745	27.251066878482877	27.884479718889054	22.41452754194062
44-45	22.49586519480302	27.420672786241102	27.706196817181734	22.377265201774144
46-47	22.57397923579994	27.36398430944996	27.42978084787424	22.632255606875862
48-49	22.510567983824494	27.422347469447843	27.51000620514616	22.5570783415815
50-51	22.444303595319422	27.583714008761746	27.40876515834983	22.563217237569003
52-53	22.59955458829594	27.508312731069783	27.308297228935935	22.58383545169834
54-55	22.62796750802428	27.39926344600687	27.30655562363199	22.666213422336863
56-57	22.617869203242453	27.498533885924974	27.275972508492107	22.607624402340466
58-59	22.514763474634865	27.579116381970227	27.365972950028443	22.540147193366465
60-61	22.617306207835888	27.552920214864706	27.341598308122038	22.48817526917737
62-63	22.610563378618167	27.63033464736161	27.24316981959724	22.515932154422984
64-65	22.491829280589428	27.65023131943465	27.225551523107804	22.632387876868123
66-67	22.623301897935242	27.687149158067285	27.033787801830673	22.6557611421668
68-69	22.529574401992804	27.618388512515217	27.180814852006662	22.671222233485317
70-71	22.53621588778184	27.373737168752125	27.21126859649206	22.878778346973974
72-73	22.662460622546472	27.391734257463952	27.136440841225056	22.809364278764523
74-75	22.48235872173817	27.606543572288295	27.154643790595767	22.756453915377765
76-77	22.6550689751642	27.500499797429995	27.021856040250768	22.822575187155035
78-79	22.5762555005078	27.512811906745544	27.018732869107996	22.892199723638658
80-81	22.610687388438983	27.513258399722424	27.06019584531572	22.815858366522875
82-83	22.64326264254993	27.50751023281397	27.19488420993844	22.654342914697654
84-85	22.77386759885727	27.544697219865256	27.015642525569184	22.665792655708294
86-87	22.57052611556155	27.551579548052203	27.127121373163117	22.750772963223138
88-89	22.63594311122283	27.536561199796978	27.106505161832732	22.72099052714746
90-91	22.69411048006843	27.585528246085524	27.083571524011685	22.636789749834357
92-93	22.787426756567662	27.52708129970614	27.12609426007685	22.559397683649347
94-95	22.498254708981374	27.62140389003644	27.232273429432325	22.64806797154986
96-97	22.55125493588801	27.739296361589943	27.336604549140304	22.372844153381745
98-99	23.70066096140772	29.659203603203068	27.456772035536027	19.18336339985319
100	22.64982975755311	30.650095634882252	21.224105188570512	25.475969418994122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1812.0
1	1610.5
2	2061.5
3	2895.0
4	3702.0
5	5616.5
6	7445.0
7	8892.0
8	9907.5
9	9961.0
10	9239.0
11	8373.0
12	8885.5
13	11397.5
14	17171.0
15	25434.0
16	32474.0
17	37021.0
18	39028.0
19	38369.0
20	36065.5
21	33760.5
22	33080.0
23	35183.5
24	40854.0
25	50111.5
26	62827.5
27	77109.0
28	92832.5
29	111641.5
30	129519.0
31	146931.5
32	165787.0
33	183497.0
34	201538.5
35	219924.0
36	240165.0
37	260103.5
38	273863.0
39	284199.0
40	297130.0
41	308572.5
42	316331.0
43	325428.5
44	333829.0
45	342878.5
46	350222.0
47	355145.5
48	358212.0
49	355375.5
50	351321.0
51	345731.0
52	334528.0
53	318713.5
54	302015.0
55	283530.0
56	263403.5
57	241087.0
58	217177.0
59	191799.0
60	162149.0
61	132931.5
62	106534.0
63	83410.0
64	64407.5
65	49378.0
66	37641.5
67	28937.5
68	22214.0
69	16478.5
70	11939.0
71	8562.0
72	6107.0
73	4252.0
74	2835.5
75	1890.0
76	1300.5
77	852.0
78	564.5
79	392.0
80	266.5
81	182.5
82	130.5
83	85.0
84	55.0
85	39.0
86	29.0
87	22.0
88	15.5
89	9.0
90	6.5
91	5.5
92	4.0
93	2.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.60728498465206E-4
20-21	0.0
22-23	6.162552608170978E-4
24-25	0.007014432101591659
26-27	0.029073684061239103
28-29	0.027939932846667584
30-31	0.02884908824397182
32-33	0.049672190812615716
34-35	0.024386340363867345
36-37	0.019462072082383514
38-39	0.015309566435223283
40-41	0.007628930840019729
42-43	0.010569570796442846
44-45	0.004822435710376583
46-47	0.0035399359948047665
48-49	0.008530600706432067
50-51	0.002061655590024676
52-53	0.002068264668980682
54-55	0.0010751197834092446
56-57	0.006423354430730213
58-59	0.004172108433098176
60-61	0.00782271643226363
62-63	0.006076930939437693
64-65	0.0012233037482026845
66-67	0.004462031082845557
68-69	0.011634427874783282
70-71	0.015105130581599381
72-73	0.004990884028592896
74-75	0.0016247091308653156
76-77	0.0025028230830772746
78-79	0.0026935539698957033
80-81	0.003763434962358844
82-83	0.0033523802102421304
84-85	0.004137888937909208
86-87	4.171413387483841E-5
88-89	2.794818643371317E-4
90-91	0.004287248397490256
92-93	7.621937196814451E-4
94-95	0.0023040793546363035
96-97	5.226045252600897E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	506.0
22-23	1250.0
24-25	2916.0
26-27	4572.0
28-29	9362.0
30-31	22265.0
32-33	29185.0
34-35	40304.0
36-37	60678.0
38-39	67915.0
40-41	69394.0
42-43	80416.0
44-45	89613.0
46-47	88957.0
48-49	82313.0
50-51	73959.0
52-53	70495.0
54-55	76301.0
56-57	81193.0
58-59	79641.0
60-61	78441.0
62-63	77137.0
64-65	81873.0
66-67	85248.0
68-69	86099.0
70-71	82572.0
72-73	78557.0
74-75	80261.0
76-77	81586.0
78-79	85526.0
80-81	85323.0
82-83	82001.0
84-85	83377.0
86-87	88313.0
88-89	93720.0
90-91	100164.0
92-93	104604.0
94-95	126455.0
96-97	293579.0
98-99	514309.0
100-101	4693894.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.8122717787066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4394979895681	75.85567011242333
2	7.222781079261351	12.251609437848268
3	1.9038323237509935	4.8440503338916745
4	0.6673017195837491	2.263814991989408
5	0.3062999585663979	1.2988997665869957
6	0.17169063153059022	0.8736883501938113
7	0.08919225673794585	0.5295218543310387
8	0.06031386789016723	0.40922849244206955
9	0.036414871328079725	0.27795851674773553
>10	0.10126060937993382	1.261707169180686
>50	0.0010231874252095417	0.056502532353920026
>100	3.685758163325208E-4	0.06107151862810802
>500	2.2929161057919222E-5	0.016276923382957578
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.929481305188624E-5	0.0	0.0	0.0
3	0.0	4.929481305188624E-5	0.0	0.0	0.0
4	0.0	9.858962610377248E-5	0.0	0.0	0.0
5	0.0	1.1091332936674404E-4	0.0	0.0	0.0
6	0.0	1.1091332936674404E-4	2.464740652594312E-5	0.0	1.232370326297156E-5
7	0.0	1.1091332936674404E-4	2.464740652594312E-5	0.0	1.232370326297156E-5
8	0.0	1.232370326297156E-4	2.464740652594312E-5	0.0	2.464740652594312E-5
9	0.0	2.711214717853743E-4	3.697110978891468E-5	2.464740652594312E-5	2.464740652594312E-5
10-11	0.0	2.711214717853743E-4	5.545666468337203E-5	7.394221957782937E-5	2.464740652594312E-5
12-13	0.0	2.772833234168601E-4	7.394221957782937E-5	7.394221957782937E-5	2.464740652594312E-5
14-15	0.0	4.128440593095473E-4	7.394221957782937E-5	7.394221957782937E-5	4.3132961420400465E-5
16-17	0.0	4.1900591094103307E-4	8.010407120931514E-5	8.010407120931514E-5	4.929481305188624E-5
18-19	0.0	4.4365331746697617E-4	9.242777447228669E-5	9.858962610377248E-5	6.778036794634359E-5
20-21	0.0	4.6830072399291927E-4	9.858962610377248E-5	1.4172258752417294E-4	7.394221957782937E-5
22-23	0.0	5.730522017281776E-4	9.858962610377248E-5	1.9717925220754496E-4	7.394221957782937E-5
24-25	0.0	6.16185163148578E-4	1.1091332936674404E-4	2.834451750483459E-4	8.626592284080092E-5
26-27	0.0	6.408325696745211E-4	1.293988842612014E-4	3.3890183973171793E-4	8.626592284080092E-5
28-29	0.0	6.531562729374927E-4	1.3556073589268716E-4	9.057921898284097E-4	8.626592284080092E-5
30-31	0.0	7.086129376208647E-4	1.3556073589268716E-4	0.0028529373053779164	8.626592284080092E-5
32-33	0.0	7.332603441468078E-4	1.3556073589268716E-4	0.007098453079471619	8.626592284080092E-5
34-35	0.0	8.811447833024667E-4	1.4172258752417294E-4	0.01228057030155116	8.626592284080092E-5
36-37	0.0	0.0010167055191951538	1.6020814241863028E-4	0.02164042292977806	8.626592284080092E-5
38-39	0.0	0.0010536766289840683	1.6020814241863028E-4	0.045191019865316706	8.626592284080092E-5
40-41	0.0	0.001121456996930412	1.7253184568160183E-4	0.07038066933483059	8.626592284080092E-5
42-43	0.0	0.0011399425518248693	1.7253184568160183E-4	0.08628440839569537	8.626592284080092E-5
44-45	0.0	0.0012262084746656704	1.848555489445734E-4	0.1062118365719204	8.626592284080092E-5
46-47	0.0	0.0013063125458749854	1.848555489445734E-4	0.12783993579843547	9.858962610377248E-5
48-49	6.16185163148578E-6	0.0013247981007694428	1.848555489445734E-4	0.14786595360076427	1.1091332936674404E-4
50-51	1.232370326297156E-5	0.001515815501345502	1.848555489445734E-4	0.16679516181268858	1.1091332936674404E-4
52-53	1.232370326297156E-5	0.0015281392046084736	1.848555489445734E-4	0.2159236048705247	1.293988842612014E-4
54-55	1.232370326297156E-5	0.001540462907871445	1.9717925220754496E-4	0.2746214035120582	1.4788443915565873E-4
56-57	1.232370326297156E-5	0.001565110314397388	1.9717925220754496E-4	0.3305771781775806	1.4788443915565873E-4
58-59	1.232370326297156E-5	0.0015712721660288739	1.9717925220754496E-4	0.3667410854027707	1.4788443915565873E-4
60-61	1.232370326297156E-5	0.001626728830712246	1.9717925220754496E-4	0.39180749783965485	1.4788443915565873E-4
62-63	1.232370326297156E-5	0.0016636999405011607	1.9717925220754496E-4	0.41185200119687804	1.4788443915565873E-4
64-65	1.232370326297156E-5	0.0017869369731308763	1.9717925220754496E-4	0.42108245494084373	1.4788443915565873E-4
66-67	1.232370326297156E-5	0.001793098824762362	1.9717925220754496E-4	0.42979531314776465	1.4788443915565873E-4
68-69	1.232370326297156E-5	0.0018054225280253336	1.9717925220754496E-4	0.4375592462034368	1.4788443915565873E-4
70-71	1.232370326297156E-5	0.0018115843796568194	1.9717925220754496E-4	0.44421404596544134	1.4788443915565873E-4
72-73	1.232370326297156E-5	0.0018115843796568194	1.9717925220754496E-4	0.4489340243151595	1.4788443915565873E-4
74-75	1.232370326297156E-5	0.0018423936378142484	1.9717925220754496E-4	0.4528036671397325	1.4788443915565873E-4
76-77	1.232370326297156E-5	0.0019409832639180208	1.9717925220754496E-4	0.45637754108599426	1.4788443915565873E-4
78-79	1.232370326297156E-5	0.0019717925220754497	1.9717925220754496E-4	0.4592489639462667	1.4788443915565873E-4
80-81	1.232370326297156E-5	0.0019717925220754497	1.9717925220754496E-4	0.4600992994714117	1.6020814241863028E-4
82-83	1.232370326297156E-5	0.0019717925220754497	2.0334110383903075E-4	0.46057992389866764	1.7253184568160183E-4
84-85	1.232370326297156E-5	0.001996439928601393	2.0950295547051654E-4	0.46083255981555854	1.7253184568160183E-4
86-87	1.232370326297156E-5	0.002045734741653279	2.0950295547051654E-4	0.4609557968481882	1.7253184568160183E-4
88	1.232370326297156E-5	0.002181295477545966	2.0950295547051654E-4	0.4609804442547142	1.7253184568160183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9180	0.0	46.104877	1
GTATCAA	16110	0.0	35.56871	1
ATCAACG	19410	0.0	28.839397	3
TCAACGC	19410	0.0	28.81808	4
CAACGCA	19815	0.0	28.249945	5
TATCAAC	20560	0.0	27.64888	2
AACGCAG	20385	0.0	27.439732	6
ACGCAGA	23415	0.0	23.835907	7
CGCAGAG	24050	0.0	23.223763	8
GTACATG	24065	0.0	21.799538	1
TACATGG	23925	0.0	21.667711	2
ACATGGG	24240	0.0	20.686354	3
GCAGAGT	28305	0.0	19.601059	9
CATGGGG	18995	0.0	19.058235	4
GTGGTAT	4905	0.0	18.72526	1
GAGTACT	15570	0.0	18.09563	12-13
AGAGTAC	25445	0.0	17.527914	10-11
CAGAGTA	27665	0.0	17.467312	10-11
TGGTATC	4910	0.0	16.768164	2
GTACTTT	17260	0.0	16.38414	14-15
>>END_MODULE