FastQCFastQC Report
Fri 27 May 2016
SRR2140220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6458618
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.045.1494641
GTATCAA122450.035.1296961
TCAACGC145200.028.9735154
ATCAACG146550.028.818973
CAACGCA148350.028.3583055
TATCAAC153650.027.6480222
AACGCAG153200.027.6217526
ACGCAGA178550.023.5617717
GTACATG185000.022.6735421
CGCAGAG187400.022.4270978
TACATGG186600.021.8614462
ACATGGG190550.021.084233
GCAGAGT222200.018.8961359
CATGGGG148900.018.7988434
GAGTACT121050.018.36316512-13
GTGGTAT37750.017.7740971
TGGTATC35950.017.6335092
AGAGTAC195750.017.08592610-11
CAGAGTA214150.016.80964910-11
GTACTTT137100.015.97378814-15
Produced by FastQC (version 0.11.5)