##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6458618
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36871696081112	33.0	31.0	34.0	31.0	34.0
2	32.65696841026981	34.0	31.0	34.0	31.0	34.0
3	32.328488385595804	33.0	31.0	34.0	30.0	34.0
4	36.19027058110574	37.0	35.0	37.0	35.0	37.0
5	36.075182647433245	37.0	35.0	37.0	35.0	37.0
6	36.168512365958165	37.0	36.0	37.0	35.0	37.0
7	36.14790749352261	37.0	36.0	37.0	35.0	37.0
8	36.15224123798621	37.0	37.0	37.0	35.0	37.0
9	37.976133903568844	39.0	38.0	39.0	35.0	39.0
10-11	37.90288719661079	39.0	38.0	39.0	35.0	39.0
12-13	37.866713049138376	39.0	38.0	39.0	35.0	39.0
14-15	39.25690410858793	41.0	39.0	41.0	36.0	41.0
16-17	39.245996744195125	41.0	39.0	41.0	36.0	41.0
18-19	39.21736422559749	41.0	39.0	41.0	36.0	41.0
20-21	39.208473187487684	40.5	39.0	41.0	36.0	41.0
22-23	39.148004073483776	40.0	39.0	41.0	36.0	41.0
24-25	39.09017357441422	40.0	39.0	41.0	35.5	41.0
26-27	38.934136407867626	40.0	38.0	41.0	35.0	41.0
28-29	38.8149933733839	40.0	38.0	41.0	35.0	41.0
30-31	38.68520787268822	40.0	38.0	41.0	35.0	41.0
32-33	38.528579796102946	40.0	38.0	41.0	34.0	41.0
34-35	38.618243780777206	40.0	38.0	41.0	35.0	41.0
36-37	38.60857497090179	40.0	38.0	41.0	34.5	41.0
38-39	38.558572034900315	40.0	38.0	41.0	34.0	41.0
40-41	38.48628912682826	40.0	38.0	41.0	34.0	41.0
42-43	38.45524399919966	40.0	38.0	41.0	34.0	41.0
44-45	38.498940547532285	40.0	38.0	41.0	34.0	41.0
46-47	38.448597497885686	40.0	38.0	41.0	34.0	41.0
48-49	38.360821424966986	40.0	38.0	41.0	34.0	41.0
50-51	38.26171919090667	40.0	37.5	41.0	34.0	41.0
52-53	38.05665183008446	40.0	37.0	41.0	33.0	41.0
54-55	37.9436269604504	40.0	37.0	41.0	33.0	41.0
56-57	37.79465563594457	40.0	36.5	41.0	33.0	41.0
58-59	37.597381543931576	39.0	36.0	41.0	33.0	41.0
60-61	37.397375458503134	39.0	35.5	41.0	32.5	41.0
62-63	37.112382465821156	39.0	35.0	40.5	32.0	41.0
64-65	36.7960376969852	38.0	35.0	40.0	31.5	41.0
66-67	36.508493850008065	37.5	35.0	40.0	31.0	41.0
68-69	36.21672748286823	37.0	35.0	40.0	31.0	41.0
70-71	35.91117110589856	36.5	35.0	39.0	31.0	41.0
72-73	35.57633222282504	36.0	34.0	39.0	31.0	40.5
74-75	35.23515198105936	35.5	34.0	38.5	30.5	40.0
76-77	34.05638877191181	35.0	32.5	36.5	29.5	39.0
78-79	34.66576345941558	35.0	34.0	37.0	30.0	39.0
80-81	34.53274574047708	35.0	34.0	36.5	30.0	39.0
82-83	34.309105540788345	35.0	34.0	36.0	30.0	38.0
84-85	34.11590686120901	35.0	34.0	36.0	30.0	37.0
86-87	33.90808259369162	35.0	34.0	35.5	30.0	37.0
88-89	33.75190651598794	35.0	34.0	35.0	30.0	36.5
90-91	33.596886789025056	35.0	34.0	35.0	30.0	36.0
92-93	33.48876989378532	35.0	34.0	35.0	30.0	36.0
94-95	33.46361628908048	35.0	33.0	35.0	30.0	36.0
96-97	33.483333158049945	35.0	33.0	35.0	30.0	35.0
98-99	33.54476337908223	35.0	33.0	35.0	30.0	35.0
100	32.40790627004467	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	18.0
21	171.0
22	661.0
23	2314.0
24	6080.0
25	13139.0
26	24208.0
27	38736.0
28	56713.0
29	76565.0
30	98523.0
31	125418.0
32	158218.0
33	206546.0
34	287639.0
35	419440.0
36	703729.0
37	1338917.0
38	1790478.0
39	1062648.0
40	48455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35200162016085	19.75806588963769	13.612122593409302	24.277809896792164
2	15.359880395465408	20.741573506901943	40.297227673164755	23.601318424467895
3	18.556167898457534	25.274741438493496	30.038934025824098	26.130156637224864
4	12.913784342099191	17.283078206514148	37.90219517550039	31.90094227588627
5	13.652626614548188	37.49981807253502	35.24746315697879	13.600092155938004
6	31.341844338835333	36.857265749421934	18.186568705565183	13.614321206177543
7	27.595903643782616	32.08859852061231	22.42773918507024	17.887758650534835
8	25.28056311737279	36.1033118849884	20.623978690177992	17.99214630746082
9	25.86921846128692	16.401759633407643	20.936924896316828	36.79209700898861
10-11	24.91592628639749	26.324478394603922	28.27064551580539	20.48894980319319
12-13	25.39200336666451	23.81530383125306	28.48841656218095	22.304276239901476
14-15	22.471123079271756	25.248497433971167	26.07048442871215	26.209895058044925
16-17	22.28344360976296	28.402345517260812	26.744939861747515	22.569271011228718
18-19	22.328431433267724	27.6619844225917	28.278698550642062	21.730885593498513
20-21	23.06591540049207	26.806274899627308	28.189350972295124	21.9384587275855
22-23	22.820308954786256	26.75340888300563	27.98198725106055	22.44429491114757
24-25	22.549096900172746	26.90000391867556	28.14693267606973	22.403966505081964
26-27	22.50310906649431	27.054562344406225	28.03511259473945	22.407215994360016
28-29	22.445034732862783	27.189902656173814	27.99049855522705	22.37456405573635
30-31	22.79322324900895	26.94546448213069	27.884695747760723	22.376616521099635
32-33	22.544258492240292	27.068821849258008	27.89177081426555	22.49514884423615
34-35	22.660520024626827	27.1420641024909	27.91685794747409	22.28055792540819
36-37	22.666876882213916	27.05470946157992	27.821099331330462	22.4573143248757
38-39	22.45997500190179	27.236853450652156	27.921036977655838	22.382134569790217
40-41	22.695634295069546	27.062659782377036	27.807261766141544	22.434444156411875
42-43	22.59822549143649	27.144044340576762	27.602763643015116	22.654966524971627
44-45	22.61429872934344	27.26887496669515	27.5009348227986	22.615891481162812
46-47	22.693895637709844	27.182988672232174	27.330104150648893	22.793011539409093
48-49	22.665849197111385	27.35084819576974	27.2330797103086	22.75022289681028
50-51	22.661229289707112	27.385847054489133	27.199783005625815	22.753140650177937
52-53	22.790708759362957	27.333122683632766	27.095325994079083	22.78084256292519
54-55	22.73920355442173	27.21502539710806	27.197131117272587	22.848639931197614
56-57	22.799796503290075	27.326882990089658	27.070610427322155	22.80271007929812
58-59	22.66287127210304	27.409884966440018	27.190389823796853	22.736853937660097
60-61	22.804358955318726	27.37096602600993	27.164099684265093	22.660575334406253
62-63	22.760011448644338	27.435524428513265	27.12565103784705	22.678813084995355
64-65	22.673029152466864	27.441059213295127	27.057721573994026	22.828190060243987
66-67	22.77062604196438	27.460977058943804	26.885657430765868	22.882739468325948
68-69	22.680010116581332	27.44179805472112	26.990550210370394	22.887641618327155
70-71	22.67795849963942	27.255104822874888	27.02200277650606	23.04493390097963
72-73	22.744711511768664	27.261609065205878	26.96479169664458	23.02888772638088
74-75	22.635171962519944	27.38951309513865	26.980325566591844	22.994989375749558
76-77	22.80450286466607	27.374663103569784	26.799376070667346	23.021457961096797
78-79	22.69874533354489	27.34109104988629	26.862884804794735	23.097278811774085
80-81	22.766644494311443	27.336459992929402	26.87889462276203	23.018000889997126
82-83	22.849501964445082	27.353394950914517	27.000287229568233	22.79681585507217
84-85	22.91608592070066	27.384709730299235	26.84110551226655	22.858098836733554
86-87	22.699066843687618	27.471577697469225	26.931772764996253	22.897582693846903
88-89	22.772747479402234	27.337425619774542	26.94263332765158	22.94719357317164
90-91	22.77572136530305	27.39407056446345	26.946042397902747	22.884165672330752
92-93	22.918939604871888	27.353192520945193	26.999054575889257	22.72881329829366
94-95	22.704509567698743	27.475082782569533	27.080107234754415	22.740300414977312
96-97	22.70655401454201	27.56863028798821	27.149141173628127	22.575674523841656
98-99	23.83363159502986	29.525481205066196	27.31077217062729	19.330115029276655
100	30.621540707545936	41.0858368911863	28.292622401267764	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1529.0
1	1356.0
2	1667.0
3	2318.0
4	2977.5
5	4447.0
6	5856.5
7	7034.5
8	7886.5
9	7871.5
10	7264.5
11	6650.5
12	7007.5
13	8945.0
14	13671.0
15	20148.0
16	25441.5
17	28914.0
18	30581.0
19	30158.5
20	28311.0
21	26353.0
22	25712.5
23	27675.0
24	32340.0
25	39614.5
26	49438.5
27	61036.5
28	73666.0
29	88034.5
30	102646.0
31	115978.5
32	129573.5
33	143065.5
34	156195.5
35	169337.0
36	183767.0
37	197930.5
38	209337.5
39	219069.5
40	229194.0
41	239121.0
42	246641.0
43	254283.0
44	262075.0
45	268576.5
46	274857.0
47	281923.0
48	287183.0
49	287526.5
50	284630.0
51	280135.5
52	271204.0
53	258882.0
54	245919.0
55	231194.0
56	215572.0
57	197670.5
58	178365.0
59	156216.0
60	132568.5
61	111003.0
62	90876.0
63	73060.5
64	58078.5
65	46461.5
66	37331.0
67	28599.5
68	20944.5
69	15914.0
70	11955.0
71	8550.5
72	6076.5
73	4227.5
74	2854.5
75	1976.5
76	1360.0
77	861.0
78	527.5
79	338.5
80	233.5
81	157.0
82	115.0
83	80.0
84	48.5
85	32.0
86	23.5
87	18.0
88	12.5
89	7.0
90	3.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.26428409297469E-4
20-21	0.0
22-23	6.8133450822243E-4
24-25	0.005993820324781427
26-27	0.027354005452208733
28-29	0.02624901837508808
30-31	0.027885104671644158
32-33	0.04835374126557973
34-35	0.022952338589145162
36-37	0.0185084443499139
38-39	0.01430796369044895
40-41	0.007386569344280715
42-43	0.0102758334209849
44-45	0.004516676787670861
46-47	0.0035134521817091332
48-49	0.0083814556028776
50-51	0.001962180659326532
52-53	0.0019390330426606061
54-55	0.0011302430876791684
56-57	0.006864751229527537
58-59	0.0044908152241282945
60-61	0.007683934517170917
62-63	0.005668039361103657
64-65	0.0011502735642668794
66-67	0.00403921084726838
68-69	0.011057350479061348
70-71	0.014967441546354869
72-73	0.0046489620615879895
74-75	0.001400275173519863
76-77	0.002324218653824374
78-79	0.0025445940100257006
80-81	0.0033186604024928
82-83	0.0029337588035844376
84-85	0.0035766260418093033
86-87	3.163598204890016E-5
88-89	2.1413578864283735E-4
90-91	0.0037014054454205993
92-93	6.869318201333291E-4
94-95	0.0022587591574331416
96-97	5.912671237045599E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	112.0
20-21	410.0
22-23	952.0
24-25	2217.0
26-27	3538.0
28-29	8038.0
30-31	18596.0
32-33	23800.0
34-35	32890.0
36-37	49848.0
38-39	56328.0
40-41	56977.0
42-43	65352.0
44-45	72966.0
46-47	71973.0
48-49	67421.0
50-51	59798.0
52-53	56847.0
54-55	62056.0
56-57	65264.0
58-59	64325.0
60-61	63715.0
62-63	62155.0
64-65	66261.0
66-67	69030.0
68-69	69376.0
70-71	66840.0
72-73	63626.0
74-75	64954.0
76-77	65106.0
78-79	68778.0
80-81	68229.0
82-83	65460.0
84-85	66478.0
86-87	70544.0
88-89	75352.0
90-91	80005.0
92-93	82689.0
94-95	99279.0
96-97	233107.0
98-99	1361582.0
100-101	2756344.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.00413559514128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64210273162226	77.95595693959167
2	6.62218666939261	11.390708805015581
3	1.525626775648003	3.9363063624122723
4	0.5288401730267146	1.8192976779659018
5	0.244841424153869	1.0528687521118427
6	0.12690174418607167	0.6548444888543306
7	0.07927505936051366	0.4772588068188143
8	0.05467547524711594	0.37618535895054
9	0.03647817482297619	0.28235465043646624
>10	0.13598727652314677	1.8210679621448334
>50	0.002575020376079978	0.1380035474713858
>100	4.8040475601726683E-4	0.07709508652804893
>500	2.90708846080761E-5	0.018051561698300588
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.741594254374542E-5	0.0	3.096637701749817E-5	0.0
3	0.0	7.741594254374542E-5	0.0	4.644956552624725E-5	0.0
4	0.0	1.85798262104989E-4	0.0	4.644956552624725E-5	0.0
5	0.0	1.85798262104989E-4	0.0	4.644956552624725E-5	0.0
6	0.0	1.85798262104989E-4	0.0	4.644956552624725E-5	1.5483188508749084E-5
7	0.0	2.0128145061373812E-4	0.0	4.644956552624725E-5	1.5483188508749084E-5
8	0.0	2.4773101613998535E-4	0.0	4.644956552624725E-5	1.5483188508749084E-5
9	0.0	5.109452207887198E-4	0.0	4.644956552624725E-5	3.096637701749817E-5
10-11	0.0	5.109452207887198E-4	0.0	8.515753679811996E-5	3.096637701749817E-5
12-13	0.0	5.109452207887198E-4	0.0	9.28991310524945E-5	3.096637701749817E-5
14-15	1.5483188508749084E-5	6.580355116218361E-4	0.0	1.2386550806999267E-4	5.4191159780621795E-5
16-17	4.644956552624725E-5	6.812602943849597E-4	0.0	1.2386550806999267E-4	6.193275403499634E-5
18-19	4.644956552624725E-5	7.354514541655815E-4	0.0	1.470902908331163E-4	6.193275403499634E-5
20-21	4.644956552624725E-5	8.051258024549525E-4	0.0	2.4773101613998535E-4	6.193275403499634E-5
22-23	4.644956552624725E-5	8.593169622355743E-4	0.0	3.019221759206071E-4	6.967434828937088E-5
24-25	6.193275403499634E-5	9.444744990336942E-4	0.0	3.2514695868373077E-4	7.741594254374542E-5
26-27	7.741594254374542E-5	9.909240645599414E-4	0.0	5.186868150430944E-4	8.515753679811996E-5
28-29	7.741594254374542E-5	0.0010064072530686905	0.0	0.001083823195612436	9.28991310524945E-5
30-31	7.741594254374542E-5	0.001099306384121185	0.0	0.003421784660433548	1.0064072530686905E-4
32-33	7.741594254374542E-5	0.0011302727611386832	0.0	0.008167381938365142	1.083823195612436E-4
34-35	8.515753679811996E-5	0.0012851046462261742	0.0	0.014352915747610403	1.083823195612436E-4
36-37	9.28991310524945E-5	0.0014476781255680395	0.0	0.025570485822199115	1.083823195612436E-4
38-39	9.28991310524945E-5	0.0015018692853486612	0.0	0.05162869208242382	1.083823195612436E-4
40-41	9.28991310524945E-5	0.001641217981927403	0.0	0.0798545447338734	1.083823195612436E-4
42-43	9.28991310524945E-5	0.0016799259531992757	0.0	0.09796987528910983	1.2386550806999267E-4
44-45	9.28991310524945E-5	0.001950881752102385	0.0	0.12049791456933975	1.2386550806999267E-4
46-47	1.0838231956124359E-4	0.0021908711739879955	0.0	0.14455878951193585	1.2386550806999267E-4
48-49	1.2386550806999267E-4	0.0022063543624967446	0.0	0.16727262705427076	1.2386550806999267E-4
50-51	1.2386550806999267E-4	0.0024153774073648574	0.0	0.18923552995393134	1.2386550806999267E-4
52-53	1.2386550806999267E-4	0.0024153774073648574	0.0	0.24001264666837396	1.3934869657874176E-4
54-55	1.2386550806999267E-4	0.0024153774073648574	0.0	0.3026189194035009	1.5483188508749084E-4
56-57	1.2386550806999267E-4	0.0024153774073648574	0.0	0.363328501546306	1.6257347934186538E-4
58-59	1.2386550806999267E-4	0.0024308605958736065	0.0	0.4048698963152798	1.7031507359623993E-4
60-61	1.3160710232436722E-4	0.002469568567145479	0.0	0.4339163579576931	1.7031507359623993E-4
62-63	1.5483188508749084E-4	0.0025315013211804757	0.0	0.4557941033205556	2.167646391224872E-4
64-65	1.5483188508749084E-4	0.002725041177539839	0.0	0.46657814411689935	2.167646391224872E-4
66-67	1.5483188508749084E-4	0.0027637491488117115	0.0	0.4766422166475862	2.2450623337686175E-4
68-69	1.5483188508749084E-4	0.002825681902846708	0.0	0.4859321297528357	2.322478276312363E-4
70-71	1.5483188508749084E-4	0.002872131468372955	0.0	0.4934801841508508	2.786973931574835E-4
72-73	1.5483188508749084E-4	0.002895356251136079	0.0	0.4987289850553168	2.941805816662326E-4
74-75	1.5483188508749084E-4	0.002972772193679824	0.0	0.5031494353745647	2.941805816662326E-4
76-77	1.5483188508749084E-4	0.0030734129189866934	0.0	0.5074615033742513	2.941805816662326E-4
78-79	1.5483188508749084E-4	0.003127604078767315	0.0	0.5103181516541154	2.941805816662326E-4
80-81	1.5483188508749084E-4	0.0031430872672760643	0.0	0.5112471429646404	3.096637701749817E-4
82-83	1.5483188508749084E-4	0.0031585704557848134	0.0	0.511773571373938	3.2514695868373077E-4
84-85	1.5483188508749084E-4	0.0031740536442935625	0.0	0.5121219431153847	3.2514695868373077E-4
86-87	1.5483188508749084E-4	0.00322050320981981	0.0	0.512253550217709	3.2514695868373077E-4
88	1.5483188508749084E-4	0.0033134023408723044	0.0	0.5122612918119634	3.2514695868373077E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6920	0.0	45.149464	1
GTATCAA	12245	0.0	35.129696	1
TCAACGC	14520	0.0	28.973515	4
ATCAACG	14655	0.0	28.81897	3
CAACGCA	14835	0.0	28.358305	5
TATCAAC	15365	0.0	27.648022	2
AACGCAG	15320	0.0	27.621752	6
ACGCAGA	17855	0.0	23.561771	7
GTACATG	18500	0.0	22.673542	1
CGCAGAG	18740	0.0	22.427097	8
TACATGG	18660	0.0	21.861446	2
ACATGGG	19055	0.0	21.08423	3
GCAGAGT	22220	0.0	18.896135	9
CATGGGG	14890	0.0	18.798843	4
GAGTACT	12105	0.0	18.363165	12-13
GTGGTAT	3775	0.0	17.774097	1
TGGTATC	3595	0.0	17.633509	2
AGAGTAC	19575	0.0	17.085926	10-11
CAGAGTA	21415	0.0	16.809649	10-11
GTACTTT	13710	0.0	15.973788	14-15
>>END_MODULE