FastQCFastQC Report
Fri 27 May 2016
SRR2140219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780380
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97900.048.0764731
GTATCAA171000.035.6913531
TCAACGC209400.028.5045874
ATCAACG210950.028.2372423
CAACGCA213300.027.8688775
TATCAAC216850.027.6191792
AACGCAG220350.027.1435246
ACGCAGA256400.023.2159717
CGCAGAG261500.022.7631918
GTACATG263350.021.830261
TACATGG262150.021.619562
ACATGGG271200.020.5227873
GCAGAGT303800.019.459719
CATGGGG210300.018.9152814
GTGGTAT46200.018.7713341
GAGTACT163150.018.64205612-13
CGTCGAA5453.2221942E-718.30264594
CAGAGTA295900.017.6813610-11
AGAGTAC275000.017.18925510-11
GTACTTT181700.016.63856114-15
Produced by FastQC (version 0.11.5)