##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7780380
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33979407175485	33.0	31.0	34.0	31.0	34.0
2	32.629155131240374	34.0	31.0	34.0	31.0	34.0
3	32.294487801367026	33.0	31.0	34.0	30.0	34.0
4	36.169532464995285	37.0	35.0	37.0	35.0	37.0
5	36.05360984939039	37.0	35.0	37.0	35.0	37.0
6	36.145623221487895	37.0	36.0	37.0	35.0	37.0
7	36.12409985116408	37.0	36.0	37.0	35.0	37.0
8	36.128813374153964	37.0	36.0	37.0	35.0	37.0
9	37.9464861613443	39.0	38.0	39.0	35.0	39.0
10-11	37.87237185073222	39.0	38.0	39.0	35.0	39.0
12-13	37.8321623751025	39.0	38.0	39.0	35.0	39.0
14-15	39.2026625306219	41.0	39.0	41.0	36.0	41.0
16-17	39.187967554283986	40.5	39.0	41.0	36.0	41.0
18-19	39.16650741994607	40.0	39.0	41.0	36.0	41.0
20-21	39.16094250780236	40.0	39.0	41.0	36.0	41.0
22-23	39.09825246750819	40.0	39.0	41.0	35.5	41.0
24-25	39.03591655952606	40.0	38.5	41.0	35.0	41.0
26-27	38.872965204465174	40.0	38.0	41.0	35.0	41.0
28-29	38.751811711894334	40.0	38.0	41.0	35.0	41.0
30-31	38.615076182933265	40.0	38.0	41.0	34.5	41.0
32-33	38.45072014217795	40.0	38.0	41.0	34.0	41.0
34-35	38.53821947432684	40.0	38.0	41.0	34.0	41.0
36-37	38.52765128906621	40.0	38.0	41.0	34.0	41.0
38-39	38.47789658735539	40.0	38.0	41.0	34.0	41.0
40-41	38.408812661907774	40.0	38.0	41.0	34.0	41.0
42-43	38.37969342851769	40.0	38.0	41.0	34.0	41.0
44-45	38.426187673843174	40.0	38.0	41.0	34.0	41.0
46-47	38.37311354350756	40.0	38.0	41.0	34.0	41.0
48-49	38.284210008306985	40.0	38.0	41.0	34.0	41.0
50-51	38.17983275512744	40.0	37.0	41.0	33.0	41.0
52-53	37.97179768840646	40.0	37.0	41.0	33.0	41.0
54-55	37.85661536496145	40.0	37.0	41.0	33.0	41.0
56-57	37.70321402940367	39.5	36.0	41.0	33.0	41.0
58-59	37.50511774009907	39.0	36.0	41.0	33.0	41.0
60-61	37.3010656218443	39.0	35.0	41.0	32.0	41.0
62-63	37.009976009295016	38.5	35.0	40.0	32.0	41.0
64-65	36.693774723442594	38.0	35.0	40.0	31.0	41.0
66-67	36.40907192536075	37.0	35.0	40.0	31.0	41.0
68-69	36.11939936893471	37.0	35.0	39.5	31.0	41.0
70-71	35.821493365371595	36.5	34.5	39.0	31.0	41.0
72-73	35.48936540191292	36.0	34.0	39.0	31.0	40.5
74-75	35.15182038312342	35.5	34.0	38.5	30.0	40.0
76-77	33.97407770160153	34.5	32.5	36.5	28.5	39.0
78-79	34.59265083783275	35.0	34.0	37.0	30.0	39.0
80-81	34.46626157554198	35.0	34.0	36.5	30.0	39.0
82-83	34.24918235221236	35.0	34.0	36.0	30.0	37.5
84-85	34.065931793980894	35.0	34.0	36.0	30.0	37.0
86-87	33.85869112693919	35.0	34.0	35.0	30.0	37.0
88-89	33.7063719104721	35.0	34.0	35.0	30.0	36.0
90-91	33.55173546186634	35.0	34.0	35.0	30.0	36.0
92-93	33.44610347991207	35.0	33.0	35.0	30.0	36.0
94-95	33.42845715789592	35.0	33.0	35.0	30.0	36.0
96-97	33.448117522761166	35.0	33.0	35.0	30.0	35.0
98-99	33.51371636679566	35.0	33.0	35.0	30.0	35.0
100	32.35916329560361	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	39.0
21	199.0
22	858.0
23	2964.0
24	7771.0
25	16617.0
26	30246.0
27	48523.0
28	70423.0
29	94503.0
30	121937.0
31	153289.0
32	195020.0
33	253185.0
34	356121.0
35	515799.0
36	862959.0
37	1632686.0
38	2102445.0
39	1258179.0
40	56610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36647824399322	19.8596212524324	13.650965634069287	24.122934869505087
2	15.361794668126748	20.841090023880582	40.343042370681125	23.454072937311548
3	18.42359884735707	25.735491068559636	30.308082638637185	25.53282744544611
4	12.93470241813382	17.38199162508772	37.94133448494804	31.74197147183043
5	13.46686151576144	37.701860320447075	35.24868965269049	13.582588511101001
6	30.9034134579545	36.98337356273087	18.347844192700098	13.765368786614534
7	27.303319375146202	32.33849760551541	22.60641511083006	17.751767908508327
8	25.352771972577177	35.831643184523124	20.83548618447942	17.98009865842028
9	26.14062552214673	16.26587647389973	20.924672059719448	36.66882594423409
10-11	24.933184497415294	26.214291589870932	28.34416185327709	20.508362059436685
12-13	25.5106627182734	23.814363822846698	28.551124752261458	22.123848706618443
14-15	22.631516712551313	25.211435688231166	26.156530914942454	26.00051668427506
16-17	22.222500700479923	28.313379295098695	26.850802917081175	22.613317087340207
18-19	22.241148146155947	27.55155032878732	28.554752826469453	21.652548698587278
20-21	23.1051353935369	26.648388106995576	28.382544123326102	21.86393237614142
22-23	22.8747836051866	26.64483356680032	28.167584627363873	22.31279820064921
24-25	22.561531270666617	26.79634306113085	28.35367098260576	22.28845468559677
26-27	22.508683596490357	26.93825373997056	28.267002564907784	22.2860600986313
28-29	22.377377260120422	27.10198825977003	28.19891788661443	22.32171659349512
30-31	22.749038094005	26.93419964623937	28.05526052577145	22.261501733984183
32-33	22.49622975428034	27.06522339065815	28.06224284698312	22.37630400807839
34-35	22.598914510770946	27.113664491668455	28.15803788582481	22.12938311173579
36-37	22.580673634417785	27.051198671527395	28.04385246333058	22.32427523072424
38-39	22.444300015639733	27.14873375200683	28.090620492486103	22.316345739867334
40-41	22.66922874999592	26.934359481054905	27.998136053072397	22.39827571587678
42-43	22.539178572553556	27.091287305762883	27.78277642401515	22.586757697668414
44-45	22.626857016175656	27.216363938857533	27.62408347414515	22.532695570821655
46-47	22.702642913724986	27.126260868412473	27.38558585566855	22.785510362193996
48-49	22.668721373660038	27.27611643031871	27.30246474610037	22.752697449920888
50-51	22.570510017469754	27.35560343698778	27.276719870547435	22.797166674995033
52-53	22.718120435650643	27.314660533816127	27.146913831265433	22.8203051992678
54-55	22.72690323317852	27.175239346045423	27.16762395442047	22.930233466355592
56-57	22.7176566075876	27.27745449409051	27.126491461626905	22.87839743669499
58-59	22.60815029647418	27.4724786172032	27.210804207745255	22.70856687857737
60-61	22.744388022950382	27.39806710010319	27.16850625713861	22.689038619807825
62-63	22.73674946901866	27.479638435924404	27.054481169588474	22.729130925468464
64-65	22.6372051868427	27.518268581116807	27.007267440183686	22.837258791856808
66-67	22.764704304713625	27.524365538589734	26.83635015271732	22.874580003979318
68-69	22.654164305635355	27.42304290110874	27.015175318684854	22.90761747457105
70-71	22.64183184139119	27.171648649550622	27.05785971550841	23.128659793549776
72-73	22.75179578951186	27.20681592904392	27.018484814009707	23.02290346743451
74-75	22.63255667436229	27.39621228848878	26.99052911401732	22.980701923131605
76-77	22.83957138797412	27.214172280637232	26.859973922201235	23.086282409187408
78-79	22.726584945786396	27.21039669000071	26.883430614575783	23.179587749637115
80-81	22.73626502330356	27.257766991963457	26.905888411603208	23.100079573129772
82-83	22.81897079464521	27.233460461132765	27.050042866507766	22.89752587771426
84-85	22.95425074888237	27.205554309624663	26.87703383919705	22.963161102295913
86-87	22.761689052330418	27.194858226934265	27.000339688489067	23.043113032246247
88-89	22.81429119499248	27.120818415541425	27.032070254148294	23.0328201353178
90-91	22.9050262638764	27.056474920124412	27.062795690933537	22.975703125065646
92-93	22.98361373533541	27.02852247578707	27.120967089451014	22.866896699426505
94-95	22.757592708783392	27.145998807347787	27.188358859039102	22.908049624829715
96-97	22.817522867927604	27.213443180896462	27.216419917202778	22.752614033973153
98-99	23.902556855607042	29.158548194734607	27.383882561796284	19.555012387862064
100	30.796660094730143	40.658837945471255	28.544501959798602	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1222.0
1	1147.0
2	1846.0
3	2829.0
4	3876.5
5	6424.5
6	8716.0
7	10296.0
8	11411.5
9	11287.0
10	10223.0
11	9028.5
12	9473.0
13	12398.5
14	19217.0
15	28959.0
16	37205.5
17	42374.0
18	44262.5
19	42985.5
20	39759.5
21	36311.5
22	34492.0
23	35457.0
24	40030.0
25	48301.5
26	60354.5
27	74572.5
28	89638.5
29	106911.0
30	123381.0
31	138915.5
32	156045.0
33	173627.5
34	190852.0
35	207885.0
36	225670.0
37	243296.5
38	257789.5
39	267264.5
40	277285.0
41	287745.5
42	295069.0
43	303667.5
44	315003.5
45	325732.0
46	335033.0
47	343408.5
48	348905.0
49	349170.5
50	346140.0
51	340855.5
52	331280.0
53	320253.5
54	306137.5
55	287750.5
56	267741.5
57	244997.0
58	220872.0
59	192884.5
60	163088.5
61	135549.5
62	109250.0
63	85768.5
64	65830.0
65	50373.0
66	38646.5
67	29418.5
68	22259.5
69	16641.0
70	12080.0
71	8512.5
72	5920.5
73	4002.0
74	2651.5
75	1814.5
76	1300.5
77	890.0
78	598.0
79	403.0
80	269.0
81	176.0
82	112.5
83	74.5
84	51.5
85	37.5
86	32.0
87	24.5
88	22.5
89	18.0
90	8.5
91	4.5
92	4.5
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.976571838393498E-4
20-21	0.0
22-23	7.198379696147395E-4
24-25	0.006891209972403662
26-27	0.028790154371688803
28-29	0.027745398137455024
30-31	0.028852721425298655
32-33	0.05016065184012825
34-35	0.02424908231222048
36-37	0.019663260133230352
38-39	0.015109751138243737
40-41	0.0081254662569034
42-43	0.010424725105921332
44-45	0.004591701038569199
46-47	0.003555675597004837
48-49	0.00851858635947721
50-51	0.00209502435961263
52-53	0.00209616384131682
54-55	0.0010269463489960009
56-57	0.007047421093948563
58-59	0.004453045927871029
60-61	0.007922507671740799
62-63	0.0062964854749802054
64-65	0.0013458753534845481
66-67	0.004584116193312492
68-69	0.011956295942679999
70-71	0.015384489514884856
72-73	0.005171742930223349
74-75	0.0016978386267024026
76-77	0.0024739863676664694
78-79	0.0026292300494871984
80-81	0.003754608437397416
82-83	0.0036194354240264765
84-85	0.004304118926444471
86-87	3.606033615445363E-5
88-89	3.0251838303374236E-4
90-91	0.0040238443117685775
92-93	6.758158167743387E-4
94-95	0.0021869672304830187
96-97	4.7947200542762313E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	481.0
22-23	1139.0
24-25	2644.0
26-27	3226.0
28-29	10393.0
30-31	24207.0
32-33	31393.0
34-35	42924.0
36-37	64451.0
38-39	73666.0
40-41	75176.0
42-43	87356.0
44-45	96773.0
46-47	94962.0
48-49	87092.0
50-51	77249.0
52-53	73895.0
54-55	79628.0
56-57	84621.0
58-59	83152.0
60-61	82527.0
62-63	80984.0
64-65	85997.0
66-67	90670.0
68-69	90140.0
70-71	86175.0
72-73	81959.0
74-75	84266.0
76-77	85385.0
78-79	89410.0
80-81	87799.0
82-83	85338.0
84-85	86336.0
86-87	90951.0
88-89	96418.0
90-91	102583.0
92-93	106532.0
94-95	121286.0
96-97	276221.0
98-99	1592118.0
100-101	3182725.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.68580164884844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.00073652461731	74.48097983400545
2	7.4368159419891	12.447118076405877
3	1.9410606325209228	4.873176452446028
4	0.7423985654399203	2.485128763671792
5	0.3435599263739977	1.4375543926513674
6	0.18404727720126474	0.9241286360325401
7	0.10029017478324041	0.587500457196702
8	0.06652173099016839	0.44535395079850293
9	0.043567934777410146	0.32814157932290805
>10	0.13797869049173203	1.7261481299538466
>50	0.0023821063674886204	0.1309226679311361
>100	5.891106286275579E-4	0.09220062920141786
>500	2.5691909592384487E-5	0.017673394972055733
>1k	2.569190921878712E-5	0.023973035410329583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.2852842663211822E-5
3	0.0	0.0	0.0	1.2852842663211822E-5	1.2852842663211822E-5
4	0.0	8.996989864248275E-5	0.0	2.5705685326423644E-5	3.8558527989635464E-5
5	0.0	8.996989864248275E-5	0.0	2.5705685326423644E-5	3.8558527989635464E-5
6	6.426421331605912E-5	8.996989864248275E-5	0.0	2.5705685326423644E-5	7.711705597927093E-5
7	6.426421331605912E-5	8.996989864248275E-5	0.0	3.8558527989635464E-5	7.711705597927093E-5
8	6.426421331605912E-5	1.156755839689064E-4	0.0	3.8558527989635464E-5	7.711705597927093E-5
9	6.426421331605912E-5	2.1849832527460098E-4	0.0	3.8558527989635464E-5	8.996989864248275E-5
10-11	6.426421331605912E-5	2.1849832527460098E-4	0.0	3.8558527989635464E-5	8.996989864248275E-5
12-13	6.426421331605912E-5	2.1849832527460098E-4	0.0	5.783779198445321E-5	1.0282274130569458E-4
14-15	7.711705597927093E-5	3.2774748791190145E-4	0.0	9.639631997408866E-5	1.4138126929533003E-4
16-17	1.6708695462175368E-4	3.3417390924350736E-4	0.0	1.0924916263730049E-4	1.6708695462175368E-4
18-19	1.6708695462175368E-4	3.7273243723314283E-4	0.0	1.4138126929533006E-4	1.6708695462175368E-4
20-21	1.6708695462175368E-4	4.3699665054920195E-4	0.0	3.984381225595665E-4	1.6708695462175368E-4
22-23	1.6708695462175368E-4	4.819815998704434E-4	0.0	0.0013302692156424236	1.799397972849655E-4
24-25	1.6708695462175368E-4	5.14113706528473E-4	0.0	0.003264622036455803	2.0564548261138915E-4
26-27	1.6708695462175368E-4	5.398193918548966E-4	0.0	0.007975188872522937	2.0564548261138915E-4
28-29	1.6708695462175368E-4	5.462458131865025E-4	0.0	0.02621337261162051	2.0564548261138915E-4
30-31	1.6708695462175368E-4	6.812006611502265E-4	0.0	0.02866826556029397	2.0564548261138915E-4
32-33	1.6708695462175368E-4	6.812006611502265E-4	0.0	0.03376441767625746	2.0564548261138915E-4
34-35	1.6708695462175368E-4	8.611404584351922E-4	0.0	0.04021654469318979	2.1849832527460098E-4
36-37	1.6708695462175368E-4	0.0010475066770517637	0.0	0.05102578537295094	2.1849832527460098E-4
38-39	1.6708695462175368E-4	0.001086065205041399	0.0	0.07901285027209468	2.4420401060102465E-4
40-41	1.6708695462175368E-4	0.0013109899516476058	0.0	0.10903066431202589	2.699096959274483E-4
42-43	1.799397972849655E-4	0.0013495484796372413	0.0	0.1288176155920405	2.763361172590542E-4
44-45	1.799397972849655E-4	0.001523061855590601	0.0	0.15302594474819997	2.827625385906601E-4
46-47	1.799397972849655E-4	0.0016451638608911132	0.0	0.17805042941347338	3.2774748791190145E-4
48-49	1.799397972849655E-4	0.0016451638608911132	0.0	0.2017574977057676	3.8558527989635466E-4
50-51	1.8636621861657142E-4	0.001882941450160532	0.0	0.22437207437168877	3.8558527989635466E-4
52-53	1.9279263994817733E-4	0.0019022207141553497	0.0	0.27929227107159293	3.984381225595665E-4
54-55	1.9921906127978324E-4	0.0019150735568185615	0.0	0.34660260809883325	4.2414380788599013E-4
56-57	2.0564548261138915E-4	0.0019150735568185615	0.0	0.40973577126052974	4.4342307188080786E-4
58-59	2.0564548261138915E-4	0.0019279263994817733	0.0	0.4520601821504862	4.498494932124138E-4
60-61	2.3135116793781283E-4	0.001953632084808197	0.0	0.48072202128944863	4.627023358756256E-4
62-63	2.699096959274483E-4	0.001992190612797833	0.0	0.5042105912564682	4.884080212020493E-4
64-65	2.699096959274483E-4	0.0022878059940517046	0.0	0.5156560476480583	4.884080212020493E-4
66-67	2.827625385906601E-4	0.0023135116793781283	0.0	0.5256684120827004	5.07687285196867E-4
68-69	2.827625385906601E-4	0.0023520702073677635	0.0	0.5354237196640781	5.269665491916848E-4
70-71	2.827625385906601E-4	0.002377775892694187	0.0	0.5433282179019534	5.719514985129262E-4
72-73	3.1489464524868963E-4	0.002397055156689005	0.0	0.5490991442577355	6.683478184870147E-4
74-75	3.2132106658029554E-4	0.002454892948673458	0.0	0.5534241258139063	6.747742398186206E-4
76-77	3.3417390924350736E-4	0.002538436425984335	0.0	0.5572799786128698	6.812006611502265E-4
78-79	3.3417390924350736E-4	0.0025705685326423643	0.0	0.5602811173747297	6.812006611502265E-4
80-81	3.3417390924350736E-4	0.0025705685326423643	0.0	0.5612258013104758	7.004799251450443E-4
82-83	3.3417390924350736E-4	0.0025705685326423643	0.0	0.5618812962862996	7.19759189139862E-4
84-85	3.534531732383251E-4	0.002596274217968788	0.0	0.5621062210329058	7.19759189139862E-4
86-87	3.59879594569931E-4	0.002609127060632	0.0	0.5622154701955431	7.261856104714679E-4
88	3.7273243723314283E-4	0.002724802644600906	0.0	0.5622218966168747	7.326120318030738E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9790	0.0	48.076473	1
GTATCAA	17100	0.0	35.691353	1
TCAACGC	20940	0.0	28.504587	4
ATCAACG	21095	0.0	28.237242	3
CAACGCA	21330	0.0	27.868877	5
TATCAAC	21685	0.0	27.619179	2
AACGCAG	22035	0.0	27.143524	6
ACGCAGA	25640	0.0	23.215971	7
CGCAGAG	26150	0.0	22.763191	8
GTACATG	26335	0.0	21.83026	1
TACATGG	26215	0.0	21.61956	2
ACATGGG	27120	0.0	20.522787	3
GCAGAGT	30380	0.0	19.45971	9
CATGGGG	21030	0.0	18.915281	4
GTGGTAT	4620	0.0	18.771334	1
GAGTACT	16315	0.0	18.642056	12-13
CGTCGAA	545	3.2221942E-7	18.302645	94
CAGAGTA	29590	0.0	17.68136	10-11
AGAGTAC	27500	0.0	17.189255	10-11
GTACTTT	18170	0.0	16.638561	14-15
>>END_MODULE