FastQCFastQC Report
Fri 27 May 2016
SRR2140218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6109343
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65950.047.1917271
GTATCAA117950.035.1344641
TCAACGC138500.028.8875064
ATCAACG139450.028.690713
CAACGCA143000.027.9490095
TATCAAC145650.027.7007332
AACGCAG147150.027.1607746
ACGCAGA170900.023.3862387
CGCAGAG175800.022.7823148
CTTATAC55100.020.713521
GTACATG169700.020.4246311
TACATGG166600.020.3749352
TGGTATC34300.019.522672
GTGGTAT38000.019.395041
ACATGGG169050.019.3073543
GCAGAGT209850.018.8849959
GAGTACT120150.018.36723312-13
TATACAC75700.018.3035893
CATGGGG129650.017.8984454
AGAGTAC186950.017.33482210-11
Produced by FastQC (version 0.11.5)