##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6109343
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34464720674547	33.0	31.0	34.0	31.0	34.0
2	32.63823245805646	34.0	31.0	34.0	31.0	34.0
3	32.300610392966966	33.0	31.0	34.0	30.0	34.0
4	36.17779833281582	37.0	35.0	37.0	35.0	37.0
5	36.059330602325	37.0	35.0	37.0	35.0	37.0
6	36.15261051802133	37.0	36.0	37.0	35.0	37.0
7	36.13216331117765	37.0	36.0	37.0	35.0	37.0
8	36.13270510429681	37.0	36.0	37.0	35.0	37.0
9	37.95025946325161	39.0	38.0	39.0	35.0	39.0
10-11	37.87590335000016	39.0	38.0	39.0	35.0	39.0
12-13	37.84134079556509	39.0	38.0	39.0	35.0	39.0
14-15	39.219062180663286	41.0	39.0	41.0	36.0	41.0
16-17	39.203515664450336	40.5	39.0	41.0	36.0	41.0
18-19	39.17757891478675	40.0	39.0	41.0	36.0	41.0
20-21	39.167591506449114	40.0	39.0	41.0	36.0	41.0
22-23	39.106380737319434	40.0	39.0	41.0	36.0	41.0
24-25	39.0480544286495	40.0	39.0	41.0	35.5	41.0
26-27	38.8909174215741	40.0	38.0	41.0	35.0	41.0
28-29	38.766138215847306	40.0	38.0	41.0	35.0	41.0
30-31	38.63160531845907	40.0	38.0	41.0	34.5	41.0
32-33	38.468387075194585	40.0	38.0	41.0	34.0	41.0
34-35	38.56509922097233	40.0	38.0	41.0	34.0	41.0
36-37	38.55856228065736	40.0	38.0	41.0	34.0	41.0
38-39	38.50844769014924	40.0	38.0	41.0	34.0	41.0
40-41	38.44381213204662	40.0	38.0	41.0	34.0	41.0
42-43	38.41167816251124	40.0	38.0	41.0	34.0	41.0
44-45	38.46124106533088	40.0	38.0	41.0	34.0	41.0
46-47	38.41288005534247	40.0	38.0	41.0	34.0	41.0
48-49	38.33575956652437	40.0	38.0	41.0	34.0	41.0
50-51	38.2406109032199	40.0	38.0	41.0	33.0	41.0
52-53	38.046526134855014	40.0	37.0	41.0	33.0	41.0
54-55	37.94004864099365	40.0	37.0	41.0	33.0	41.0
56-57	37.79856951278535	40.0	37.0	41.0	33.0	41.0
58-59	37.614694618544284	39.0	36.0	41.0	33.0	41.0
60-61	37.420919214774145	39.0	36.0	41.0	32.0	41.0
62-63	37.14047136349588	39.0	35.0	40.5	32.0	41.0
64-65	36.83562820645044	38.5	35.0	40.0	31.0	41.0
66-67	36.5510539598601	38.0	35.0	40.0	31.0	41.0
68-69	36.265882192817685	37.0	35.0	40.0	31.0	41.0
70-71	35.961007917339074	37.0	35.0	39.0	31.0	41.0
72-73	35.61928012114184	36.0	34.0	39.0	30.5	41.0
74-75	35.27320914065692	36.0	34.0	39.0	30.0	40.0
76-77	34.10361422798165	35.0	32.5	37.0	28.5	39.0
78-79	34.71314703173714	35.0	34.0	37.0	30.0	39.0
80-81	34.56810937139634	35.0	34.0	37.0	30.0	39.0
82-83	34.33307184918071	35.0	34.0	36.0	30.0	38.5
84-85	34.1243361745405	35.0	34.0	36.0	30.0	37.0
86-87	33.89280013747961	35.0	34.0	35.5	30.0	37.0
88-89	33.72087849226223	35.0	34.0	35.0	30.0	36.5
90-91	33.55174575107448	35.0	33.5	35.0	30.0	36.0
92-93	33.43758025920181	35.0	33.0	35.0	29.0	36.0
94-95	33.407465199850094	35.0	33.0	35.0	30.0	36.0
96-97	33.425782823866896	35.0	33.0	35.0	30.0	35.5
98-99	33.49093782787148	35.0	33.0	35.0	30.0	35.0
100	32.31775249884524	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	24.0
21	135.0
22	617.0
23	2160.0
24	5858.0
25	12862.0
26	23847.0
27	38676.0
28	56752.0
29	77184.0
30	99910.0
31	125034.0
32	157886.0
33	203728.0
34	280490.0
35	405357.0
36	668474.0
37	1270141.0
38	1723527.0
39	918810.0
40	37869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57432313098152	20.151250306293164	14.339348764670767	23.935077798054554
2	14.759475773417863	20.912821558717525	41.263569585141965	23.064133082722645
3	18.222352223471493	25.68083016455288	30.48301593150033	25.613801680475294
4	12.626414984393575	17.845143086580666	38.46652577863119	31.06191615039457
5	13.336082128634782	37.60261946333673	35.68896033501475	13.372338073013742
6	30.442225948027474	37.646159333335845	18.461297720556857	13.450316998079826
7	26.827516477631065	32.46502938204648	22.71763101204172	17.989823128280733
8	24.547287654335335	36.74113566712492	20.995563680088022	17.716012998451717
9	25.264877090711717	17.08689461370887	21.234607387406466	36.41362090817294
10-11	24.210794843242557	26.868535618314443	28.710885933233737	20.209783605209267
12-13	24.896760584566948	24.19188118918843	28.953948075922405	21.95741015032222
14-15	22.0185132836706	25.762573815220392	26.450888417952633	25.76802448315637
16-17	21.788300313143328	29.032974576808012	27.132426514602308	22.046298595446352
18-19	21.786476238053883	28.10383045144571	28.78757423510952	21.322119075390887
20-21	22.42350037157196	27.288637754985125	28.763352855866092	21.524509017576825
22-23	22.130783710072137	27.28458885696094	28.591785889006687	21.992841543960235
24-25	22.020583283638594	27.422982974761624	28.70775169199543	21.848682049604356
26-27	21.950463295629437	27.592622264075395	28.61281794950685	21.844096490788314
28-29	21.802519304960754	27.515218799391526	28.6526061184713	22.029655777176416
30-31	22.05690970141289	27.37424778543105	28.677851026624086	21.89099148653197
32-33	21.88466018026504	27.563614176985475	28.600996550402453	21.950729092347032
34-35	22.001243901409453	27.680052829049632	28.61543232430388	21.70327094523703
36-37	22.019895163557983	27.570362032667017	28.446243366362427	21.963499437412572
38-39	21.86069883900791	27.628876441981703	28.61368704834947	21.896737670660922
40-41	22.180329862538187	27.479948712904108	28.45439174393644	21.885329680621265
42-43	22.104070702219715	27.559029516052668	28.3650268746436	21.971872907084016
44-45	22.05844967657862	27.770369575109992	28.168973053458927	22.002207694852462
46-47	22.2549333157915	27.58809469300423	27.875795170831623	22.281176820372643
48-49	22.169024421627988	27.65330477313383	27.94807659224511	22.229594212993074
50-51	22.022663970809106	27.86746625195191	27.937279155838873	22.172590621400108
52-53	22.193772038544225	27.842343167362237	27.888265136467822	22.075619657625715
54-55	22.181856252569183	27.735257562075898	27.79776057607826	22.285125609276655
56-57	22.277574516235923	27.89587406000078	27.64760718921242	22.178944234550876
58-59	22.107694978502295	27.98279380514189	27.892861791298216	22.0166494250576
60-61	22.285683270954525	28.028536001185405	27.677337288619142	22.008443439240928
62-63	22.11872958065299	28.220451292562686	27.632848399513794	22.027970727270528
64-65	22.111877230045714	28.109334292630063	27.626891525619758	22.151896951704465
66-67	22.197739866486323	28.086190117559575	27.519724473903366	22.19634554205074
68-69	22.12896590906367	28.015064161726382	27.64287101251591	22.213098916694037
70-71	22.16493031642418	27.750986447715192	27.712572491439253	22.371510744421375
72-73	22.249278515169436	27.775105430464254	27.597223029799707	22.378393024566606
74-75	22.077409154910228	27.93348262918575	27.60402098575983	22.385087230144187
76-77	22.24118711510735	27.856589070670996	27.549992742159784	22.35223107206187
78-79	22.18176789232392	27.885065139888383	27.508467988788233	22.42469897899947
80-81	22.136051859000517	27.933149022014252	27.522583983639404	22.408215135345827
82-83	22.240171210457564	27.873099198193362	27.607634727652485	22.27909486369658
84-85	22.325960763711905	27.86967865269495	27.538314326008713	22.266046257584428
86-87	22.151528079551788	27.882656767893323	27.634115423313222	22.33169972924167
88-89	22.171860185019092	27.91111438553332	27.623527577753293	22.293497851694298
90-91	22.217318097853628	27.902421565675805	27.63333345241803	22.246926884052534
92-93	22.208352429027215	27.872668264660273	27.698130875016897	22.220848431295618
94-95	22.037061237758728	27.99139520878625	27.736779602222366	22.23476395123266
96-97	22.108632631944182	28.120038887618016	27.78839215828227	21.98293632215553
98-99	23.159552656290984	30.055917963627042	28.038846741430852	18.74568263865112
100	22.181240685192645	31.21198542620282	21.795088714284994	24.81168517431954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1889.0
1	1501.0
2	1602.0
3	2188.0
4	2722.0
5	4081.0
6	5416.5
7	6642.5
8	7701.0
9	7858.0
10	7187.5
11	6424.5
12	6807.5
13	8834.5
14	13522.5
15	20051.0
16	25713.5
17	29334.5
18	30536.0
19	29731.0
20	28093.0
21	26508.5
22	26208.0
23	28442.0
24	33599.5
25	41755.0
26	52665.0
27	64591.0
28	76956.5
29	91389.0
30	105330.5
31	117724.5
32	131332.5
33	144907.5
34	158090.0
35	170759.0
36	184112.0
37	196621.5
38	205915.0
39	214761.5
40	225986.0
41	235421.0
42	240864.0
43	246518.0
44	252313.5
45	259789.5
46	264095.0
47	264424.5
48	262314.0
49	257376.0
50	252943.0
51	245997.0
52	235437.0
53	222274.5
54	208173.0
55	193812.0
56	178046.0
57	161006.5
58	143472.5
59	125355.0
60	106373.0
61	87577.0
62	70027.0
63	54550.0
64	41355.0
65	30790.0
66	23281.0
67	18106.5
68	13852.0
69	10145.5
70	7344.0
71	5303.0
72	3724.5
73	2553.5
74	1740.5
75	1149.0
76	776.0
77	522.5
78	344.0
79	240.0
80	168.0
81	110.5
82	78.5
83	58.0
84	36.5
85	22.5
86	14.5
87	8.0
88	3.0
89	1.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	6.056297706643742E-4
20-21	0.0
22-23	5.074687532253936E-4
24-25	0.0067210281322917734
26-27	0.02841647513525669
28-29	0.027321043070550898
30-31	0.02754419382182748
32-33	0.04917343367182762
34-35	0.023768743177738912
36-37	0.018817172616473483
38-39	0.014752761273909712
40-41	0.008067701319886041
42-43	0.010152357515779233
44-45	0.004592370171082068
46-47	0.0033062952814232856
48-49	0.008233806541208628
50-51	0.002010740529279822
52-53	0.002053776766556975
54-55	0.001138361166861428
56-57	0.006501977044132508
58-59	0.0042531752782817905
60-61	0.007270036714146119
62-63	0.0062492200099280755
64-65	0.0013236467425288357
66-67	0.004448361025566315
68-69	0.011438249083070453
70-71	0.014760489493884815
72-73	0.005001835800987231
74-75	0.001442992315374075
76-77	0.0024059111539937875
78-79	0.0025217472965103757
80-81	0.0036814675941109574
82-83	0.0030519337041638992
84-85	0.0042526233370210244
86-87	2.108873549411329E-5
88-89	3.73709833533624E-4
90-91	0.0035074626138585693
92-93	6.923858329945589E-4
94-95	0.001922771438035169
96-97	4.696550166257876E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	350.0
22-23	793.0
24-25	1889.0
26-27	3255.0
28-29	7181.0
30-31	15890.0
32-33	19735.0
34-35	26980.0
36-37	40067.0
38-39	44858.0
40-41	45165.0
42-43	52923.0
44-45	58513.0
46-47	57884.0
48-49	52091.0
50-51	46876.0
52-53	44770.0
54-55	48337.0
56-57	50709.0
58-59	51581.0
60-61	50466.0
62-63	49863.0
64-65	53224.0
66-67	56390.0
68-69	56819.0
70-71	52231.0
72-73	49197.0
74-75	50261.0
76-77	50706.0
78-79	53776.0
80-81	53883.0
82-83	52420.0
84-85	53832.0
86-87	58390.0
88-89	62342.0
90-91	68242.0
92-93	73402.0
94-95	92127.0
96-97	225376.0
98-99	411609.0
100-101	3764839.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.32863810276386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.03224262021472	79.49721771463727
2	6.419065756608801	11.21136540833468
3	1.4999355728191768	3.9296199244856305
4	0.5005774626458774	1.7485899231120645
5	0.20799091291385072	0.9081781581258573
6	0.12369739093300552	0.6481394812226725
7	0.06628875295645503	0.4052234562052445
8	0.04679542384272236	0.3269264506901238
9	0.02875106080446078	0.22597118856569828
>10	0.07320327306808336	0.9453074529952252
>50	0.001061224662895079	0.06246433609846144
>100	3.6336311222844E-4	0.07247879458380413
>500	2.7185417717781905E-5	0.018517710943279395
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6368372180118223E-5	0.0	0.0	0.0
3	0.0	1.6368372180118223E-5	0.0	0.0	0.0
4	0.0	4.910511654035467E-5	0.0	1.6368372180118223E-5	0.0
5	0.0	4.910511654035467E-5	0.0	1.6368372180118223E-5	0.0
6	0.0	4.910511654035467E-5	0.0	3.2736744360236446E-5	1.6368372180118223E-5
7	0.0	4.910511654035467E-5	0.0	4.910511654035467E-5	1.6368372180118223E-5
8	0.0	4.910511654035467E-5	0.0	4.910511654035467E-5	1.6368372180118223E-5
9	0.0	9.821023308070934E-5	0.0	8.184186090059111E-5	3.2736744360236446E-5
10-11	0.0	9.821023308070934E-5	0.0	1.391311635310049E-4	3.2736744360236446E-5
12-13	0.0	9.821023308070934E-5	0.0	1.47315349621064E-4	3.2736744360236446E-5
14-15	0.0	9.821023308070934E-5	0.0	2.0460465225147779E-4	7.3657674810532E-5
16-17	3.2736744360236446E-5	9.821023308070934E-5	0.0	2.291572105216551E-4	8.184186090059111E-5
18-19	3.2736744360236446E-5	1.0639441917076845E-4	0.0	2.3734139661171424E-4	8.184186090059111E-5
20-21	3.2736744360236446E-5	1.1457860526082755E-4	0.0	2.782623270620098E-4	8.184186090059111E-5
22-23	3.2736744360236446E-5	1.1457860526082755E-4	0.0	3.109990714222462E-4	1.0639441917076845E-4
24-25	3.2736744360236446E-5	1.47315349621064E-4	0.0	4.41946048863192E-4	1.3094697744094578E-4
26-27	3.2736744360236446E-5	1.47315349621064E-4	0.0	5.15603723673724E-4	1.3094697744094578E-4
28-29	3.2736744360236446E-5	1.47315349621064E-4	0.0	0.0010148390751673298	1.391311635310049E-4
30-31	3.2736744360236446E-5	1.47315349621064E-4	0.0	0.0027662548984399796	1.47315349621064E-4
32-33	3.2736744360236446E-5	1.47315349621064E-4	0.0	0.0065637172442274076	1.9642046616141868E-4
34-35	4.0920930450295554E-5	1.7186790789124133E-4	0.0	0.011073203779849976	1.9642046616141868E-4
36-37	4.910511654035467E-5	1.9642046616141868E-4	0.0	0.018193445678201404	2.0460465225147779E-4
38-39	4.910511654035467E-5	1.9642046616141868E-4	0.0	0.03396437227374531	2.20973024431596E-4
40-41	4.910511654035467E-5	1.9642046616141868E-4	0.0	0.04965345700838862	2.291572105216551E-4
42-43	4.910511654035467E-5	2.0460465225147779E-4	0.0	0.06192155195738723	2.291572105216551E-4
44-45	4.910511654035467E-5	2.4552558270177335E-4	0.0	0.07520448598155317	2.291572105216551E-4
46-47	4.910511654035467E-5	2.782623270620098E-4	0.0	0.08885570837977178	2.5370976879183246E-4
48-49	4.910511654035467E-5	2.782623270620098E-4	0.0	0.10195859030995641	2.6189395488189157E-4
50-51	5.7289302630413784E-5	2.94630699242128E-4	0.0	0.11457860526082755	2.782623270620098E-4
52-53	6.547348872047289E-5	2.94630699242128E-4	0.0	0.13843550771334986	2.864465131520689E-4
54-55	7.3657674810532E-5	2.94630699242128E-4	0.0	0.16673642321277427	2.94630699242128E-4
56-57	8.184186090059111E-5	2.94630699242128E-4	0.0	0.1965759656971298	3.028148853321871E-4
58-59	8.184186090059111E-5	2.94630699242128E-4	0.0	0.22376383188830615	3.109990714222462E-4
60-61	9.002604699065022E-5	2.94630699242128E-4	0.0	0.23688308219067092	3.109990714222462E-4
62-63	1.3094697744094578E-4	2.94630699242128E-4	0.0	0.25132817063962526	3.2736744360236443E-4
64-65	1.3094697744094578E-4	3.2736744360236443E-4	0.0	0.2589640162616504	3.2736744360236443E-4
66-67	1.3094697744094578E-4	3.3555162969242354E-4	0.0	0.2651921818761854	3.2736744360236443E-4
68-69	1.3094697744094578E-4	3.8465674623277825E-4	0.0	0.2711502693497484	3.2736744360236443E-4
70-71	1.554995357111231E-4	4.0920930450295557E-4	0.0	0.27597075495679324	3.4373581578248265E-4
72-73	1.8005209398130043E-4	4.0920930450295557E-4	0.0	0.2800464796296427	3.7647256014271914E-4
74-75	1.8005209398130043E-4	4.173934905930147E-4	0.0	0.28317283871604526	3.7647256014271914E-4
76-77	1.8005209398130043E-4	4.501302349532511E-4	0.0	0.2863728554772583	3.7647256014271914E-4
78-79	1.8005209398130043E-4	4.583144210433102E-4	0.0	0.28837798106932283	3.7647256014271914E-4
80-81	1.8005209398130043E-4	4.583144210433102E-4	0.0	0.2892782415392293	3.9284093232283735E-4
82-83	1.8005209398130043E-4	4.583144210433102E-4	0.0	0.28985113456553346	4.41946048863192E-4
84-85	1.8005209398130043E-4	4.583144210433102E-4	0.0	0.2899575289847042	4.583144210433102E-4
86-87	1.8005209398130043E-4	4.7468279322342844E-4	0.0	0.29001481828733466	4.910511654035467E-4
88	1.8005209398130043E-4	5.401562819439014E-4	0.0	0.29001481828733466	5.237879097637831E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6595	0.0	47.191727	1
GTATCAA	11795	0.0	35.134464	1
TCAACGC	13850	0.0	28.887506	4
ATCAACG	13945	0.0	28.69071	3
CAACGCA	14300	0.0	27.949009	5
TATCAAC	14565	0.0	27.700733	2
AACGCAG	14715	0.0	27.160774	6
ACGCAGA	17090	0.0	23.386238	7
CGCAGAG	17580	0.0	22.782314	8
CTTATAC	5510	0.0	20.71352	1
GTACATG	16970	0.0	20.424631	1
TACATGG	16660	0.0	20.374935	2
TGGTATC	3430	0.0	19.52267	2
GTGGTAT	3800	0.0	19.39504	1
ACATGGG	16905	0.0	19.307354	3
GCAGAGT	20985	0.0	18.884995	9
GAGTACT	12015	0.0	18.367233	12-13
TATACAC	7570	0.0	18.303589	3
CATGGGG	12965	0.0	17.898445	4
AGAGTAC	18695	0.0	17.334822	10-11
>>END_MODULE