FastQCFastQC Report
Fri 27 May 2016
SRR2140217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6222149
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.046.1437341
GTATCAA117500.034.9074131
TCAACGC137000.029.2681124
ATCAACG138150.029.054713
CAACGCA140700.028.5281335
TATCAAC145650.027.7593272
AACGCAG145350.027.701686
ACGCAGA167750.023.8781197
CGCAGAG173400.023.1241728
CTTATAC60750.022.4825481
GTACATG162500.021.6422671
TACATGG158700.021.3709182
ACATGGG160300.020.6364863
GTGGTAT34850.020.4945031
GCAGAGT202650.019.7658759
TATACAC87050.019.1446823
TGGTATC34550.018.9799752
GAGTACT119350.018.51302112-13
CATGGGG122700.018.0075724
CAGAGTA199750.017.67953710-11
Produced by FastQC (version 0.11.5)