##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6222149
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3590834935004	33.0	31.0	34.0	31.0	34.0
2	32.650688371493516	34.0	31.0	34.0	31.0	34.0
3	32.31750011129595	33.0	31.0	34.0	30.0	34.0
4	36.18453993949679	37.0	35.0	37.0	35.0	37.0
5	36.06999269866408	37.0	35.0	37.0	35.0	37.0
6	36.16244516163145	37.0	36.0	37.0	35.0	37.0
7	36.14159786273199	37.0	36.0	37.0	35.0	37.0
8	36.14459505871685	37.0	36.0	37.0	35.0	37.0
9	37.96717806018467	39.0	38.0	39.0	35.0	39.0
10-11	37.892672370912365	39.0	38.0	39.0	35.0	39.0
12-13	37.859402113321295	39.0	38.0	39.0	35.0	39.0
14-15	39.24349384754367	41.0	39.0	41.0	36.0	41.0
16-17	39.23052863247087	41.0	39.0	41.0	36.0	41.0
18-19	39.20396972171512	40.5	39.0	41.0	36.0	41.0
20-21	39.19656949501437	40.0	39.0	41.0	36.0	41.0
22-23	39.13378940946451	40.0	39.0	41.0	36.0	41.0
24-25	39.076804045135475	40.0	39.0	41.0	35.5	41.0
26-27	38.920906785802025	40.0	38.0	41.0	35.0	41.0
28-29	38.8031646981667	40.0	38.0	41.0	35.0	41.0
30-31	38.66979595289436	40.0	38.0	41.0	35.0	41.0
32-33	38.510516701303125	40.0	38.0	41.0	34.0	41.0
34-35	38.60108666874183	40.0	38.0	41.0	34.5	41.0
36-37	38.59266084227085	40.0	38.0	41.0	34.5	41.0
38-39	38.543912317506646	40.0	38.0	41.0	34.0	41.0
40-41	38.480651865592385	40.0	38.0	41.0	34.0	41.0
42-43	38.45250481359797	40.0	38.0	41.0	34.0	41.0
44-45	38.499617966872385	40.0	38.0	41.0	34.0	41.0
46-47	38.45323921044059	40.0	38.0	41.0	34.0	41.0
48-49	38.37588037652086	40.0	38.0	41.0	34.0	41.0
50-51	38.28221942691468	40.0	38.0	41.0	33.5	41.0
52-53	38.093249390334314	40.0	37.0	41.0	33.0	41.0
54-55	37.99104214819509	40.0	37.0	41.0	33.0	41.0
56-57	37.85161062360031	40.0	37.0	41.0	33.0	41.0
58-59	37.66857424106985	39.0	36.0	41.0	33.0	41.0
60-61	37.472984301882775	39.0	36.0	41.0	32.5	41.0
62-63	37.19574980848897	39.0	35.0	41.0	32.0	41.0
64-65	36.895896472428575	38.5	35.0	40.0	31.5	41.0
66-67	36.6180755431936	38.0	35.0	40.0	31.0	41.0
68-69	36.333087924012645	37.0	35.0	40.0	31.0	41.0
70-71	36.03306400178605	37.0	35.0	39.5	31.0	41.0
72-73	35.69637346452291	36.0	34.5	39.0	31.0	41.0
74-75	35.35073608387978	36.0	34.0	39.0	30.0	40.0
76-77	34.16644516459406	35.0	32.5	37.0	29.5	39.0
78-79	34.77070108076748	35.0	34.0	37.0	30.0	39.0
80-81	34.62477313133692	35.0	34.0	37.0	30.0	39.0
82-83	34.388114280367176	35.0	34.0	36.0	30.0	38.5
84-85	34.17521667638235	35.0	34.0	36.0	30.0	37.0
86-87	33.94476533595227	35.0	34.0	36.0	30.0	37.0
88-89	33.77309839054281	35.0	34.0	35.0	30.0	36.5
90-91	33.603144198935176	35.0	34.0	35.0	30.0	36.0
92-93	33.49059427519402	35.0	33.5	35.0	30.0	36.0
94-95	33.46094230075265	35.0	33.0	35.0	30.0	36.0
96-97	33.47516412580796	35.0	33.0	35.0	30.0	35.5
98-99	33.53623412143035	35.0	33.0	35.0	30.0	35.0
100	32.37892425530004	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	13.0
21	153.0
22	667.0
23	2240.0
24	6126.0
25	13041.0
26	23678.0
27	38389.0
28	56312.0
29	75909.0
30	98998.0
31	123596.0
32	155739.0
33	202249.0
34	277690.0
35	400956.0
36	661917.0
37	1271148.0
38	1770233.0
39	999812.0
40	43278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.608309283496745	20.15998009690864	14.069736999226473	24.16197362036814
2	14.82278871817438	20.91050857187766	41.11225880318842	23.154443906759546
3	18.149243934852734	25.590017211095393	30.665176934850003	25.59556191920187
4	12.501082825242532	17.864165580091377	38.57675217999441	31.057999414671684
5	13.176894349524575	37.7315136619197	35.82717160903733	13.264420379518393
6	30.46953713258876	37.76227473819736	18.45190463937781	13.316283489836067
7	26.78899203474555	32.49194128909481	22.74820162615842	17.970865050001215
8	24.60773600889339	36.75289678855328	21.02174023797887	17.61762696457446
9	25.297015548807977	16.817453262530357	21.169743765377525	36.71578742328414
10-11	24.1815086716824	26.770573960861434	28.878744305223165	20.169173062233
12-13	24.840340531864474	24.13903138610149	29.009382449697043	22.011245632336994
14-15	21.971990706104915	25.7594361690792	26.445348705085653	25.823224419730224
16-17	21.784563500488336	28.99124562912267	27.175265330354513	22.04892554003448
18-19	21.784765281499155	28.130436498086226	28.7420685237122	21.34272969670242
20-21	22.41133919670779	27.25319162016134	28.730475899264825	21.60499328386605
22-23	22.125335291288728	27.31549495809341	28.559616912825554	21.99955283779231
24-25	21.95652830979797	27.449163199625982	28.73963706120995	21.8546714293661
26-27	21.932903376708907	27.56483754529477	28.621419902488004	21.880839175508317
28-29	21.74595607171394	27.56635698290427	28.628065489732606	22.059621455649175
30-31	22.026990188354663	27.393163333832426	28.669437558067894	21.910408919745016
32-33	21.85119608517178	27.547958667826016	28.59815178047843	22.00269346652377
34-35	21.959658698356233	27.75422261789443	28.64440390851768	21.64171477523166
36-37	22.010225606034773	27.60178203292832	28.416309285770662	21.97168307526625
38-39	21.851128219658726	27.628186965052308	28.590812131450782	21.92987268383818
40-41	22.13572463036272	27.476474838529075	28.509553761977752	21.878246769130453
42-43	22.08515171443657	27.562162016135826	28.37960027523388	21.973085994193724
44-45	22.018749375941578	27.76496105622025	28.221818281959354	21.99447128587882
46-47	22.22263120569314	27.583265808359343	27.873600422503646	22.32050256344387
48-49	22.104612095744443	27.681758022621583	27.981561422021855	22.23206845961212
50-51	22.04182444427945	27.838785702359175	27.94594898898581	22.173440864375564
52-53	22.13411397271701	27.85497543314004	27.92369195515561	22.08721863898734
54-55	22.143044436425544	27.781574337526937	27.81212422485427	22.263257001193253
56-57	22.25349743308978	27.93662913521927	27.648147338640094	22.161726093050856
58-59	22.0450934994903	28.031368446676403	27.91070526066696	22.01283279316634
60-61	22.245121820181204	28.163335291844895	27.64793430374914	21.943608584224762
62-63	22.10601876416069	28.279746870053458	27.62060510575532	21.993629260030534
64-65	22.071434711058803	28.17918286174685	27.633943699684117	22.115438727510238
66-67	22.140995632435896	28.18797819200895	27.501522624903384	22.169503550651772
68-69	22.122524918009983	28.03581842125175	27.625887444961023	22.21576921577724
70-71	22.13285876753703	27.7921235546615	27.680300283376486	22.39471739442498
72-73	22.17989210803578	27.83940362411953	27.620125946907525	22.36057832093717
74-75	22.075360818340922	27.946588924989722	27.618769236874503	22.35928101979485
76-77	22.1918192850211	27.858499721314722	27.552973650721242	22.396707342942936
78-79	22.127566107265675	27.904054315289716	27.526495976921733	22.44188360052288
80-81	22.13705699479538	27.881763933995764	27.565815312732166	22.41536375847669
82-83	22.184735578847548	27.904758904125153	27.666754300016994	22.243751217010306
84-85	22.314931484285573	27.898466809865862	27.576059591823764	22.210542114024797
86-87	22.14884759229577	27.910769735345614	27.60979480424674	22.33058786811187
88-89	22.14313518084407	27.90284943834872	27.652380935534804	22.301634445272406
90-91	22.162233781881422	27.94184340025566	27.645249266890882	22.25067355097204
92-93	22.191013998505348	27.906564802965523	27.703550506988755	22.198870691540375
94-95	22.031210959032272	28.035044128452107	27.73719984769876	22.19654506481686
96-97	22.067871478399034	28.110858692002882	27.81437925280128	22.006890576796806
98-99	23.160241870528573	30.047318989496958	28.046175694989785	18.746263444984685
100	22.115663499577238	31.185561429710585	21.773817794742346	24.924957275969835
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1853.0
1	1507.5
2	1654.5
3	2272.0
4	2835.0
5	4321.0
6	5810.5
7	7088.0
8	8193.0
9	8330.0
10	7536.5
11	6716.5
12	7160.5
13	9203.0
14	13722.0
15	20152.5
16	25697.5
17	28986.5
18	30123.5
19	29559.0
20	28172.0
21	27063.5
22	27488.0
23	30268.0
24	35838.5
25	44469.0
26	55646.5
27	68001.0
28	81404.5
29	96108.5
30	109844.5
31	122722.0
32	136084.5
33	148523.0
34	161108.0
35	173651.0
36	186784.0
37	199853.5
38	209839.0
39	218802.0
40	229249.5
41	238435.0
42	244571.0
43	251008.0
44	258872.5
45	268677.5
46	274955.5
47	277361.5
48	276106.0
49	270055.5
50	264808.0
51	257252.5
52	245059.0
53	229953.0
54	214555.0
55	199298.0
56	183631.0
57	166545.0
58	147705.5
59	128779.5
60	108393.5
61	88695.0
62	70988.5
63	55233.0
64	42382.5
65	32242.5
66	24802.0
67	19042.0
68	14180.0
69	10494.5
70	7750.5
71	5464.0
72	3770.5
73	2563.0
74	1718.0
75	1145.5
76	778.0
77	507.0
78	322.5
79	204.0
80	137.5
81	90.5
82	65.5
83	44.0
84	27.5
85	18.0
86	13.0
87	11.0
88	10.0
89	8.0
90	6.0
91	4.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.098262513482079E-4
20-21	0.0
22-23	4.7416328463676397E-4
24-25	0.006486715375709831
26-27	0.02840886247151475
28-29	0.027665150608902886
30-31	0.028179906130927097
32-33	0.049856836421635714
34-35	0.02378716694111317
36-37	0.018900387531391494
38-39	0.015188942384155739
40-41	0.007727387070290535
42-43	0.010938572140008707
44-45	0.004713477979103918
46-47	0.003595319389472068
48-49	0.007801961364756365
50-51	0.0018736012803929718
52-53	0.0021891043266460152
54-55	0.0012773308893735652
56-57	0.006475976768530586
58-59	0.004361565845573241
60-61	0.007415883670663075
62-63	0.006050026421527601
64-65	0.0011852479006646535
66-67	0.004228810356356563
68-69	0.011568215358711635
70-71	0.01499065476203133
72-73	0.005050830207012721
74-75	0.0015794483756804833
76-77	0.0024944354134796096
78-79	0.0023911489955206816
80-81	0.003511891581373131
82-83	0.0031195810836453686
84-85	0.003960953567695675
86-87	2.1159650408719807E-5
88-89	2.894465845624629E-4
90-91	0.0035570449941172786
92-93	6.298574997132491E-4
94-95	0.0019003559063005342
96-97	5.647415051352291E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	122.0
20-21	374.0
22-23	829.0
24-25	2049.0
26-27	3577.0
28-29	8047.0
30-31	17486.0
32-33	22049.0
34-35	30325.0
36-37	44822.0
38-39	49627.0
40-41	50015.0
42-43	57958.0
44-45	64012.0
46-47	62659.0
48-49	57347.0
50-51	51360.0
52-53	49725.0
54-55	53446.0
56-57	56600.0
58-59	57334.0
60-61	55612.0
62-63	55119.0
64-65	58827.0
66-67	62526.0
68-69	62423.0
70-71	56585.0
72-73	53021.0
74-75	53771.0
76-77	54236.0
78-79	58104.0
80-81	57656.0
82-83	56191.0
84-85	57331.0
86-87	60925.0
88-89	65666.0
90-91	71244.0
92-93	75211.0
94-95	92826.0
96-97	225298.0
98-99	408945.0
100-101	3740869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.52537875325822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.17499173803645	79.8012568469683
2	6.418800962527923	11.236159707740699
3	1.37853300178224	3.619698693145696
4	0.5006251395623731	1.7526961981439784
5	0.2203245669307215	0.9641995584629496
6	0.11282219586520642	0.5924883254925886
7	0.06030208504968352	0.36945739835093183
8	0.036458771712147564	0.2552854242387434
9	0.021579821197409404	0.16999038213577636
>10	0.0733129747160281	0.9547545411299692
>50	0.0013705640527903109	0.08444649543232888
>100	8.245549163539406E-4	0.16204245453197347
>500	5.3623650612202044E-5	0.03752397422601763
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.428647079971887E-5	0.0	1.6071617699929718E-5	0.0
5	0.0	6.428647079971887E-5	0.0	3.2143235399859436E-5	0.0
6	9.642970619957832E-5	6.428647079971887E-5	0.0	3.2143235399859436E-5	1.6071617699929718E-5
7	1.1250132389950803E-4	9.642970619957832E-5	0.0	3.2143235399859436E-5	1.6071617699929718E-5
8	1.1250132389950803E-4	1.1250132389950803E-4	0.0	3.2143235399859436E-5	1.6071617699929718E-5
9	1.1250132389950803E-4	1.7678779469922692E-4	0.0	4.821485309978916E-5	3.2143235399859436E-5
10-11	1.1250132389950803E-4	1.7678779469922692E-4	0.0	9.642970619957832E-5	4.01790442498243E-5
12-13	1.1250132389950803E-4	1.9285941239915663E-4	0.0	9.642970619957832E-5	4.821485309978916E-5
14-15	1.1250132389950803E-4	3.1339654514862955E-4	0.0	1.1250132389950803E-4	5.6250661949754015E-5
16-17	1.607161769992972E-4	3.214323539985944E-4	0.0	1.2857294159943774E-4	6.428647079971887E-5
18-19	1.607161769992972E-4	3.5357558939845383E-4	0.0	1.2857294159943774E-4	6.428647079971887E-5
20-21	1.607161769992972E-4	3.6964720709838355E-4	0.0	1.366087504494026E-4	7.232227964968374E-5
22-23	1.607161769992972E-4	4.178620601981727E-4	0.0	2.732175008988052E-4	9.642970619957832E-5
24-25	1.607161769992972E-4	4.741127221479267E-4	0.0	3.5357558939845383E-4	1.1250132389950803E-4
26-27	1.607161769992972E-4	5.14291766397751E-4	0.0	4.339336778981024E-4	1.1250132389950803E-4
28-29	1.607161769992972E-4	5.14291766397751E-4	0.0	0.0011250132389950805	1.1250132389950803E-4
30-31	1.607161769992972E-4	5.946498548973996E-4	0.0	0.003150037069186225	1.1250132389950803E-4
32-33	1.607161769992972E-4	5.946498548973996E-4	0.0	0.006774186860520377	1.2857294159943774E-4
34-35	1.607161769992972E-4	6.428647079971888E-4	0.0	0.011057272977551647	1.2857294159943774E-4
36-37	1.607161769992972E-4	6.91079561096978E-4	0.0	0.01856271844341883	1.2857294159943774E-4
38-39	1.607161769992972E-4	6.91079561096978E-4	0.0	0.0364745363699905	1.2857294159943774E-4
40-41	1.607161769992972E-4	7.553660318966968E-4	0.0	0.054506891429311644	1.2857294159943774E-4
42-43	1.607161769992972E-4	7.794734584465914E-4	0.0	0.06715525455915633	1.2857294159943774E-4
44-45	1.8482360354919177E-4	8.517957380962751E-4	0.0	0.08178042666609238	1.2857294159943774E-4
46-47	2.0893103009908635E-4	9.000105911960642E-4	0.0	0.09735382421732428	1.5268036814933234E-4
48-49	2.0893103009908635E-4	9.16082208895994E-4	0.0	0.11247721647295814	1.607161769992972E-4
50-51	2.4107426549894578E-4	0.0011009058124451857	0.0	0.12555951328070092	1.607161769992972E-4
52-53	2.571458831988755E-4	0.0011089416212951505	0.0	0.15137053130678807	1.8482360354919177E-4
54-55	2.6518169204884035E-4	0.0011250132389950802	0.0	0.18215571501100342	2.0893103009908635E-4
56-57	3.1339654514862955E-4	0.00114108485669501	0.0	0.21586593313660601	2.0893103009908635E-4
58-59	3.5357558939845383E-4	0.0011651922832449045	0.0	0.24584753595582493	2.0893103009908635E-4
60-61	3.5357558939845383E-4	0.0011812639009448342	0.0	0.2598137717370639	2.0893103009908635E-4
62-63	4.178620601981727E-4	0.001189299709794799	0.0	0.27525056053784636	2.0893103009908635E-4
64-65	4.339336778981024E-4	0.001317872651394237	0.0	0.28341494232941067	2.0893103009908635E-4
66-67	4.58041104447997E-4	0.001317872651394237	0.0	0.2898034103651327	2.0893103009908635E-4
68-69	4.6607691329796184E-4	0.001317872651394237	0.0	0.2962400932539545	2.0893103009908635E-4
70-71	4.6607691329796184E-4	0.001325908460244202	0.0	0.3014312257710319	2.169668389490512E-4
72-73	4.8214853099789155E-4	0.0013339442690941668	0.0	0.3056821686526633	2.732175008988052E-4
74-75	4.8214853099789155E-4	0.0013500158867940965	0.0	0.30932239006169737	2.732175008988052E-4
76-77	4.8214853099789155E-4	0.001382159122193956	0.0	0.3130992202211808	2.732175008988052E-4
78-79	4.8214853099789155E-4	0.0014143023575938153	0.0	0.31561442839121984	2.732175008988052E-4
80-81	4.901843398478564E-4	0.0014143023575938153	0.0	0.31659479707091553	2.732175008988052E-4
82-83	4.982201486978213E-4	0.001430373975293745	0.0	0.31723766177891277	2.892891185987349E-4
84-85	4.982201486978213E-4	0.001430373975293745	0.0	0.3174465928090118	2.892891185987349E-4
86-87	5.14291766397751E-4	0.0014625172106936045	0.0	0.3175590941329113	2.892891185987349E-4
88	5.14291766397751E-4	0.0015268036814933233	0.0	0.3175912373683112	2.892891185987349E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6825	0.0	46.143734	1
GTATCAA	11750	0.0	34.907413	1
TCAACGC	13700	0.0	29.268112	4
ATCAACG	13815	0.0	29.05471	3
CAACGCA	14070	0.0	28.528133	5
TATCAAC	14565	0.0	27.759327	2
AACGCAG	14535	0.0	27.70168	6
ACGCAGA	16775	0.0	23.878119	7
CGCAGAG	17340	0.0	23.124172	8
CTTATAC	6075	0.0	22.482548	1
GTACATG	16250	0.0	21.642267	1
TACATGG	15870	0.0	21.370918	2
ACATGGG	16030	0.0	20.636486	3
GTGGTAT	3485	0.0	20.494503	1
GCAGAGT	20265	0.0	19.765875	9
TATACAC	8705	0.0	19.144682	3
TGGTATC	3455	0.0	18.979975	2
GAGTACT	11935	0.0	18.513021	12-13
CATGGGG	12270	0.0	18.007572	4
CAGAGTA	19975	0.0	17.679537	10-11
>>END_MODULE