FastQCFastQC Report
Fri 27 May 2016
SRR2140216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6437468
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66350.047.6024931
GTATCAA117100.035.3439251
TCAACGC138400.029.4116744
ATCAACG139550.029.1082083
CAACGCA141800.028.6763975
TATCAAC146600.027.7665442
AACGCAG147600.027.6073026
ACGCAGA171900.023.6055167
CGCAGAG177450.022.84328
TACATGG166200.020.9015752
GTACATG170800.020.6381191
ACATGGG169300.019.8894423
GCAGAGT212450.018.9394659
GAGTACT119350.018.16021512-13
CATGGGG132250.018.1453424
GTGGTAT39500.018.1286141
TGGTATC36600.018.0510732
AGAGTAC188450.017.5742710-11
CAGAGTA206450.017.28076610-11
ATACCGT7800.016.393786
Produced by FastQC (version 0.11.5)