##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140216_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6437468 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35364960260773 33.0 31.0 34.0 31.0 34.0 2 32.64606814356203 34.0 31.0 34.0 31.0 34.0 3 32.31058546621125 33.0 31.0 34.0 30.0 34.0 4 36.18402840992763 37.0 35.0 37.0 35.0 37.0 5 36.06558898622875 37.0 35.0 37.0 35.0 37.0 6 36.15911442200567 37.0 36.0 37.0 35.0 37.0 7 36.13848612529026 37.0 36.0 37.0 35.0 37.0 8 36.1400684244178 37.0 36.0 37.0 35.0 37.0 9 37.958136646271484 39.0 38.0 39.0 35.0 39.0 10-11 37.8844695616351 39.0 38.0 39.0 35.0 39.0 12-13 37.85066737419122 39.0 38.0 39.0 35.0 39.0 14-15 39.23637958278006 41.0 39.0 41.0 36.0 41.0 16-17 39.22155923726534 41.0 39.0 41.0 36.0 41.0 18-19 39.190271237076445 40.0 39.0 41.0 36.0 41.0 20-21 39.17895397676369 40.0 39.0 41.0 36.0 41.0 22-23 39.11599616065393 40.0 39.0 41.0 36.0 41.0 24-25 39.06134886268002 40.0 39.0 41.0 35.5 41.0 26-27 38.90531934054037 40.0 38.0 41.0 35.0 41.0 28-29 38.785260329860165 40.0 38.0 41.0 35.0 41.0 30-31 38.65080496591963 40.0 38.0 41.0 35.0 41.0 32-33 38.49306423800033 40.0 38.0 41.0 34.0 41.0 34-35 38.588533696932515 40.0 38.0 41.0 34.5 41.0 36-37 38.580123077574015 40.0 38.0 41.0 34.0 41.0 38-39 38.533189504858825 40.0 38.0 41.0 34.0 41.0 40-41 38.46134684903562 40.0 38.0 41.0 34.0 41.0 42-43 38.430920712156194 40.0 38.0 41.0 34.0 41.0 44-45 38.48079205526273 40.0 38.0 41.0 34.0 41.0 46-47 38.428716759903054 40.0 38.0 41.0 34.0 41.0 48-49 38.344792513129605 40.0 38.0 41.0 34.0 41.0 50-51 38.251162517543236 40.0 38.0 41.0 33.5 41.0 52-53 38.05051439137462 40.0 37.0 41.0 33.0 41.0 54-55 37.94174009471722 40.0 37.0 41.0 33.0 41.0 56-57 37.7997911547852 40.0 36.5 41.0 33.0 41.0 58-59 37.60966423640305 39.0 36.0 41.0 33.0 41.0 60-61 37.413882250565536 39.0 36.0 41.0 32.0 41.0 62-63 37.13219106691525 39.0 35.0 40.5 32.0 41.0 64-65 36.81949145745982 38.0 35.0 40.0 31.5 41.0 66-67 36.52969030589655 37.5 35.0 40.0 31.0 41.0 68-69 36.23737496449053 37.0 35.0 40.0 31.0 41.0 70-71 35.92891497369196 37.0 35.0 39.0 31.0 41.0 72-73 35.58789501363981 36.0 34.0 39.0 31.0 40.5 74-75 35.24079354383352 36.0 34.0 38.5 30.0 40.0 76-77 34.059533592131544 35.0 32.5 37.0 28.5 39.0 78-79 34.66812345575819 35.0 34.0 37.0 30.0 39.0 80-81 34.53261468897946 35.0 34.0 37.0 30.0 39.0 82-83 34.30111499150586 35.0 34.0 36.0 30.0 38.0 84-85 34.10099108595114 35.0 34.0 36.0 30.0 37.0 86-87 33.879624447221026 35.0 34.0 35.5 30.0 37.0 88-89 33.71932339791296 35.0 34.0 35.0 30.0 36.5 90-91 33.55336063613824 35.0 34.0 35.0 30.0 36.0 92-93 33.44614353007029 35.0 33.0 35.0 30.0 36.0 94-95 33.421878427761854 35.0 33.0 35.0 30.0 36.0 96-97 33.441921911670825 35.0 33.0 35.0 30.0 35.0 98-99 33.50348272870667 35.0 33.0 35.0 30.0 35.0 100 32.35638685063663 34.0 31.0 35.0 27.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 24.0 21 160.0 22 665.0 23 2412.0 24 6298.0 25 14053.0 26 25543.0 27 40838.0 28 60027.0 29 80449.0 30 104233.0 31 130934.0 32 165430.0 33 212893.0 34 295628.0 35 428232.0 36 716812.0 37 1373758.0 38 1822287.0 39 917855.0 40 38934.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91045299176633 19.933069958561347 14.07683890622835 24.079638143443972 2 15.064354494655351 20.739473967093893 40.797530954716976 23.398640583533776 3 18.345815466577854 25.36464647280577 30.327389588577375 25.962148472039004 4 12.769585806096433 17.490805391187962 38.31473803054244 31.424870772173158 5 13.524975968812583 37.558959516381286 35.44844028739249 13.467624227413635 6 30.997901659472326 37.28524941793886 18.31512016836433 13.401728754224488 7 27.37812444271568 32.25583412608808 22.55910242971305 17.80693900148319 8 24.984108658870227 36.42227037089737 20.73201761934972 17.861603350882678 9 25.697696672045595 16.742793905926987 21.041564789137592 36.51794463288983 10-11 24.621963169370318 26.522562908273873 28.532165130762593 20.323308791593217 12-13 25.20693695098756 23.938798608396965 28.740290437171883 22.11397400344359 14-15 22.2677068064649 25.46317123440458 26.261505299909842 26.007616659220673 16-17 22.07733692812143 28.589812019259746 26.997547793635633 22.335303258983192 18-19 22.084340398205065 27.866144554113692 28.53826763663862 21.511247411042625 20-21 22.82470387693671 27.040860314608373 28.47347782205291 21.660957986402003 22-23 22.519931263416133 26.95039901366327 28.330591523948357 22.199078198972245 24-25 22.324247434028933 27.063254250046192 28.40735209314027 22.20514622278461 26-27 22.284462660686234 27.244383318249582 28.276020010985924 22.195134010078256 28-29 22.24031995393141 27.352477193785873 28.25573479327118 22.151468059011535 30-31 22.5066791445245 27.144596131215266 28.195725892502267 22.152998831757962 32-33 22.33302850791207 27.259857144484283 28.145678986533746 22.261435361069896 34-35 22.431670801788936 27.346987120074644 28.15873240488209 22.062609673254325 36-37 22.44017750705017 27.27470132292457 28.082744681739037 22.202376488286223 38-39 22.28964688933072 27.40376133628072 28.15905880038135 22.147532974007216 40-41 22.591676890373904 27.24916142878363 28.01627634272456 22.142885338117907 42-43 22.403739148723474 27.330840599420597 27.870781408355576 22.394638843500353 44-45 22.478837143266237 27.46431557560865 27.712386665001826 22.34446061612329 46-47 22.534671598790013 27.41624375929047 27.49478890930166 22.554295732617856 48-49 22.494551249948735 27.50441937377649 27.509247638684354 22.49178173759042 50-51 22.47137090006405 27.60216558719587 27.435386299304987 22.491077213435094 52-53 22.598234143810096 27.56846195102986 27.316250071498025 22.51705383366202 54-55 22.606012910021423 27.419451795812797 27.39408727898514 22.580448015180636 56-57 22.58393548745449 27.589077572262706 27.343755522902054 22.483231417380754 58-59 22.551209494477984 27.622805760419556 27.379599321570876 22.446385423531588 60-61 22.587014744757575 27.5877177481266 27.3899697036783 22.43529780343752 62-63 22.56943656364813 27.669141929500274 27.290771653918405 22.470649852933196 64-65 22.555818783570896 27.617932306752778 27.28942293120957 22.536825978466755 66-67 22.564891658978997 27.654860472180413 27.165008153661212 22.615239715179378 68-69 22.508823303888004 27.642775647042285 27.272910415864978 22.57549063320473 70-71 22.50586288864555 27.418308206753096 27.31617497416635 22.759653930435004 72-73 22.57531480672463 27.43064745042204 27.26945373139058 22.724584011462746 74-75 22.47138724309191 27.55776022701284 27.22614202988377 22.744710500011475 76-77 22.56958389309117 27.533258184659708 27.184972630416993 22.712185291832125 78-79 22.49618178299526 27.53713421518455 27.139994814398037 22.82668918742215 80-81 22.545213088391915 27.548444298966213 27.165036288871264 22.74130632377061 82-83 22.58743329827404 27.543154766741612 27.267944721670563 22.60146721331379 84-85 22.660385184990325 27.546751096320815 27.1737558839325 22.61910783475636 86-87 22.511506036416552 27.57460261330525 27.216412951845236 22.69747839843296 88-89 22.52400405793219 27.53765369170133 27.2318106166023 22.70653163376418 90-91 22.543262622149673 27.574018785162774 27.251827185524125 22.63089140716343 92-93 22.60594638439463 27.53593744037877 27.29890004870463 22.55921612652197 94-95 22.435664009071886 27.676573509404847 27.306825417734228 22.580937063789044 96-97 22.46786971135385 27.762922913362587 27.417486804680223 22.35172057060334 98-99 23.591518612753386 29.709817522758552 27.60521661243987 19.093447252048193 100 22.592187458161973 30.830324301112803 21.248064913775234 25.329423326949986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1212.0 1 1065.5 2 1339.0 3 1875.5 4 2455.0 5 3900.5 6 5272.5 7 6357.5 8 7170.5 9 7197.0 10 6613.0 11 6032.5 12 6452.0 13 8362.5 14 12985.0 15 19582.5 16 25077.5 17 28375.5 18 29552.0 19 28976.0 20 27368.0 21 25477.0 22 24493.0 23 25675.5 24 29895.5 25 37195.5 26 46891.5 27 57898.0 28 70032.5 29 84324.0 30 98238.5 31 111252.5 32 125740.5 33 139662.0 34 153740.0 35 167726.0 36 182135.0 37 195973.0 38 206261.5 39 216000.0 40 226927.0 41 237068.5 42 245074.5 43 251481.5 44 257340.0 45 264533.0 46 269482.5 47 271481.0 48 272219.0 49 271158.0 50 268827.0 51 264265.5 52 255366.5 53 241610.0 54 227521.0 55 212772.0 56 197580.5 57 181202.5 58 161563.5 59 140164.5 60 117795.5 61 96662.5 62 77958.0 63 61484.5 64 47075.0 65 35581.5 66 27083.5 67 20781.0 68 15530.0 69 11488.5 70 8452.5 71 5977.0 72 4215.5 73 2874.0 74 1953.0 75 1298.0 76 839.5 77 523.0 78 319.0 79 223.0 80 161.5 81 105.5 82 67.5 83 45.0 84 30.0 85 20.0 86 15.5 87 12.0 88 6.0 89 1.5 90 0.0 91 0.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.815557918113146E-4 20-21 0.0 22-23 5.670534048566338E-4 24-25 0.006067741006438565 26-27 0.02814098157702185 28-29 0.027082224994117797 30-31 0.028417458142041524 32-33 0.04969340657464121 34-35 0.023073699757546376 36-37 0.019474975675888715 38-39 0.014266113299107272 40-41 0.008123877963660032 42-43 0.010375345053990872 44-45 0.004571678332213178 46-47 0.0036135621036829818 48-49 0.007956608870426216 50-51 0.002131924123256494 52-53 0.0020309171912166564 54-55 0.0010770202206789386 56-57 0.006757471487130972 58-59 0.004607159474916181 60-61 0.007393183262551875 62-63 0.005885260038316322 64-65 0.0012969254163435232 66-67 0.004314106301688002 68-69 0.011189842285340269 70-71 0.015113290516526734 72-73 0.005124471697298362 74-75 0.0015362195301600586 76-77 0.002549853092431343 78-79 0.0026992048179965418 80-81 0.0036378734182573617 82-83 0.0032502561488632607 84-85 0.0042165337056261805 86-87 9.790079164538141E-6 88-89 1.984314982223018E-4 90-91 0.004087766563156899 92-93 6.240210031147856E-4 94-95 0.002040924492978647 96-97 4.1645652519567314E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 130.0 20-21 361.0 22-23 906.0 24-25 1834.0 26-27 2761.0 28-29 5063.0 30-31 10930.0 32-33 14198.0 34-35 19639.0 36-37 29432.0 38-39 34715.0 40-41 36894.0 42-43 45088.0 44-45 51252.0 46-47 51499.0 48-49 47458.0 50-51 43133.0 52-53 42230.0 54-55 46220.0 56-57 48561.0 58-59 49362.0 60-61 49351.0 62-63 49405.0 64-65 52485.0 66-67 55871.0 68-69 56755.0 70-71 55852.0 72-73 54466.0 74-75 55679.0 76-77 57196.0 78-79 61598.0 80-81 61727.0 82-83 60231.0 84-85 61558.0 86-87 66660.0 88-89 71621.0 90-91 77733.0 92-93 82319.0 94-95 102573.0 96-97 246795.0 98-99 442515.0 100-101 4033412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.91710998979472 #Duplication Level Percentage of deduplicated Percentage of total 1 90.08941259383373 77.4022197074041 2 6.934872598570091 11.9164842363312 3 1.7092881710679908 4.40571299393711 4 0.6134251096809582 2.1081485047584314 5 0.27355993279270746 1.1751739417275948 6 0.13756890428584606 0.7091713608421548 7 0.07256788268511215 0.43643759308683117 8 0.04856873284554507 0.3338308129564521 9 0.032120572045747274 0.2483736049250635 >10 0.08733091195523852 1.109690786656658 >50 8.35135874215504E-4 0.0473716601842455 >100 3.950069491223738E-4 0.06495566013407265 >500 2.722370779817061E-5 0.016162367673827907 >1k 2.7223705916450755E-5 0.026266769382266086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.106811560072998E-5 0.0 0.0 0.0 5 0.0 3.106811560072998E-5 0.0 0.0 0.0 6 0.0 3.106811560072998E-5 0.0 0.0 0.0 7 0.0 3.106811560072998E-5 0.0 1.553405780036499E-5 0.0 8 0.0 3.106811560072998E-5 0.0 1.553405780036499E-5 0.0 9 0.0 7.767028900182495E-5 0.0 4.660217340109497E-5 0.0 10-11 0.0 7.767028900182495E-5 0.0 4.660217340109497E-5 0.0 12-13 0.0 9.320434680218994E-5 0.0 5.436920230127746E-5 0.0 14-15 0.0 1.0873840460255491E-4 0.0 9.320434680218994E-5 0.0 16-17 0.0 1.165054335027374E-4 0.0 1.0873840460255491E-4 0.0 18-19 0.0 1.2427246240291991E-4 0.0 1.2427246240291991E-4 0.0 20-21 0.0 1.2427246240291991E-4 0.0 1.475735491034674E-4 0.0 22-23 0.0 1.320394913031024E-4 0.0 1.8640869360437987E-4 0.0 24-25 0.0 1.7087463580401488E-4 0.0 2.0194275140474483E-4 0.0 26-27 0.0 1.7087463580401488E-4 0.0 2.563119537060223E-4 0.0 28-29 0.0 1.786416647041974E-4 0.0 5.359249941125921E-4 0.0 30-31 0.0 2.0194275140474483E-4 0.0 0.001429133317633579 0.0 32-33 0.0 2.0194275140474483E-4 0.0 0.0035262311206828525 0.0 34-35 0.0 2.4077789590565732E-4 0.0 0.006073816599942711 0.0 36-37 0.0 2.9514709820693477E-4 0.0 0.01049325604414655 0.0 38-39 0.0 2.9514709820693477E-4 0.0 0.02090107477039109 0.0 40-41 0.0 3.2621521380766475E-4 0.0 0.03197685798205133 0.0 42-43 0.0 3.2621521380766475E-4 0.0 0.03962738144873108 0.0 44-45 0.0 3.7281738720875974E-4 0.0 0.04927403134275774 0.0 46-47 0.0 4.194195606098547E-4 0.0 0.060310979409917066 0.0 48-49 0.0 4.194195606098547E-4 0.0 0.07064889487605996 0.0 50-51 0.0 5.048568785118621E-4 0.0 0.08167030888541893 0.0 52-53 0.0 5.126239074120446E-4 0.0 0.10344129089263046 0.0 54-55 0.0 5.203909363122271E-4 0.0 0.12908025329213288 0.0 56-57 0.0 5.281579652124096E-4 0.0 0.15679301240798402 0.0 58-59 0.0 5.436920230127745E-4 0.0 0.1819581860445753 0.0 60-61 0.0 5.592260808131395E-4 0.0 0.19358542830814848 0.0 62-63 0.0 5.902941964138695E-4 0.0 0.2083505502473954 0.0 64-65 0.0 6.058282542142345E-4 0.0 0.21653699870818777 0.0 66-67 0.0 6.213623120145996E-4 0.0 0.22294479755083832 0.0 68-69 0.0 6.524304276153295E-4 0.0 0.2291584206709843 0.0 70-71 0.0 6.60197456515512E-4 0.0 0.23485165285481807 0.0 72-73 0.0 6.679644854156945E-4 0.0 0.2395429383105283 0.0 74-75 0.0 6.679644854156945E-4 0.0 0.24316237377801336 0.0 76-77 0.0 6.679644854156945E-4 0.0 0.2464555940316907 0.0 78-79 0.0 6.834985432160595E-4 0.0 0.2488167708173462 0.0 80-81 0.0 6.834985432160595E-4 0.0 0.24953133747616296 0.0 82-83 0.0 6.834985432160595E-4 0.0 0.25003619435467483 0.0 84-85 0.0 6.990326010164245E-4 0.0 0.25025367116388 0.0 86-87 0.0 7.145666588167895E-4 0.0 0.2503313414528818 0.0 88 0.0 8.233050634193444E-4 0.0 0.2503313414528818 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6635 0.0 47.602493 1 GTATCAA 11710 0.0 35.343925 1 TCAACGC 13840 0.0 29.411674 4 ATCAACG 13955 0.0 29.108208 3 CAACGCA 14180 0.0 28.676397 5 TATCAAC 14660 0.0 27.766544 2 AACGCAG 14760 0.0 27.607302 6 ACGCAGA 17190 0.0 23.605516 7 CGCAGAG 17745 0.0 22.8432 8 TACATGG 16620 0.0 20.901575 2 GTACATG 17080 0.0 20.638119 1 ACATGGG 16930 0.0 19.889442 3 GCAGAGT 21245 0.0 18.939465 9 GAGTACT 11935 0.0 18.160215 12-13 CATGGGG 13225 0.0 18.145342 4 GTGGTAT 3950 0.0 18.128614 1 TGGTATC 3660 0.0 18.051073 2 AGAGTAC 18845 0.0 17.57427 10-11 CAGAGTA 20645 0.0 17.280766 10-11 ATACCGT 780 0.0 16.39378 6 >>END_MODULE