FastQCFastQC Report
Fri 27 May 2016
SRR2140215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062691
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47850.049.8882261
GTATCAA84600.034.942461
ATCAACG101950.028.1566583
TCAACGC101800.028.1561224
CAACGCA104600.027.402425
TATCAAC106400.027.1800882
AACGCAG108150.026.5424986
GTGGTAT23300.025.3378031
TGGTATC24150.023.2059822
ACGCAGA125350.022.900457
GTACATG126850.022.5284751
CGCAGAG128100.022.408838
TACATGG126000.022.3748782
ACATGGG125600.021.4583683
GCAGAGT150950.019.0167039
GAGTACT81500.018.81816712-13
CATGGGG92550.018.5359024
CAGAGTA146900.017.56067810-11
AGAGTAC136350.017.38202710-11
GGCGTCG3502.8740033E-1017.1121988
Produced by FastQC (version 0.11.5)