FastQCFastQC Report
Fri 27 May 2016
SRR2140214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5055324
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.048.0216521
GTATCAA92650.035.2133521
TCAACGC109450.029.1484724
ATCAACG110050.029.0281523
CAACGCA112450.028.3708345
AACGCAG116450.027.4327876
TATCAAC118350.027.351322
ACGCAGA135950.023.5292117
CGCAGAG141300.022.6683968
GTACATG143900.021.609361
TACATGG144200.021.1225092
GTGGTAT28550.020.384781
ACATGGG146450.020.2758663
CATGGGG115100.019.5979584
GCAGAGT166850.018.9934649
TGGTATC27850.018.6091372
GAGTACT91750.017.80262412-13
AGAGTAC147300.017.5777210-11
CAGAGTA162100.017.54523510-11
ATGGGGG74500.016.4220685
Produced by FastQC (version 0.11.5)