FastQCFastQC Report
Fri 27 May 2016
SRR2140213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7819022
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86000.048.359881
GTATCAA150900.035.781391
TCAACGC180800.029.2530964
ATCAACG183700.028.8375423
CAACGCA187900.028.1477385
TATCAAC193250.027.566332
AACGCAG194450.027.265136
ACGCAGA227150.023.3775067
CGCAGAG235250.022.518418
GTACATG227000.021.5589471
TACATGG225400.021.0711782
TGGTATC43650.020.6325342
ACATGGG228650.020.400093
GTGGTAT47600.020.3483311
GCAGAGT276000.019.1782919
GAGTACT156250.018.13466512-13
CATGGGG171850.017.77384
TATACCG4800.017.7006685
AGAGTAC246450.017.57354410-11
CAGAGTA268600.017.36589810-11
Produced by FastQC (version 0.11.5)