##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140213_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7819022
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37188193101388	33.0	31.0	34.0	31.0	34.0
2	32.66473722672733	34.0	31.0	34.0	31.0	34.0
3	32.32792080646403	33.0	31.0	34.0	30.0	34.0
4	36.19641228787948	37.0	35.0	37.0	35.0	37.0
5	36.080400208619444	37.0	35.0	37.0	35.0	37.0
6	36.17413891404833	37.0	36.0	37.0	35.0	37.0
7	36.15444578618656	37.0	37.0	37.0	35.0	37.0
8	36.157947247110954	37.0	37.0	37.0	35.0	37.0
9	37.97793816157571	39.0	38.0	39.0	35.0	39.0
10-11	37.90653805552664	39.0	38.0	39.0	35.0	39.0
12-13	37.871150637509395	39.0	38.0	39.0	35.0	39.0
14-15	39.25734778595073	41.0	39.0	41.0	36.0	41.0
16-17	39.24445717124213	41.0	39.0	41.0	36.0	41.0
18-19	39.22001626290347	40.5	39.0	41.0	36.0	41.0
20-21	39.21006819991186	40.0	39.0	41.0	36.0	41.0
22-23	39.146441430296576	40.0	39.0	41.0	36.0	41.0
24-25	39.08912778512153	40.0	39.0	41.0	36.0	41.0
26-27	38.93226877783354	40.0	38.0	41.0	35.0	41.0
28-29	38.812684365055915	40.0	38.0	41.0	35.0	41.0
30-31	38.67975561283173	40.0	38.0	41.0	35.0	41.0
32-33	38.520809299209574	40.0	38.0	41.0	34.0	41.0
34-35	38.61885920953529	40.0	38.0	41.0	35.0	41.0
36-37	38.60902276700206	40.0	38.0	41.0	35.0	41.0
38-39	38.567714443047976	40.0	38.0	41.0	34.0	41.0
40-41	38.49695276809191	40.0	38.0	41.0	34.0	41.0
42-43	38.46335173420245	40.0	38.0	41.0	34.0	41.0
44-45	38.50727725074203	40.0	38.0	41.0	34.0	41.0
46-47	38.455481715424526	40.0	38.0	41.0	34.0	41.0
48-49	38.373191371845344	40.0	38.0	41.0	34.0	41.0
50-51	38.2809032472759	40.0	38.0	41.0	34.0	41.0
52-53	38.08252223031201	40.0	37.0	41.0	33.0	41.0
54-55	37.972194376783385	40.0	37.0	41.0	33.0	41.0
56-57	37.82980465137694	40.0	36.5	41.0	33.0	41.0
58-59	37.63949416914721	39.0	36.0	41.0	33.0	41.0
60-61	37.44178287459456	39.0	36.0	41.0	32.5	41.0
62-63	37.15931188458177	39.0	35.0	40.5	32.0	41.0
64-65	36.842971588520584	38.0	35.0	40.0	31.5	41.0
66-67	36.55445558092032	37.5	35.0	40.0	31.0	41.0
68-69	36.25461882295919	37.0	35.0	40.0	31.0	41.0
70-71	35.94067618517511	36.5	35.0	39.0	31.0	41.0
72-73	35.59801974350798	36.0	34.0	39.0	31.0	40.5
74-75	35.25175765021726	36.0	34.0	38.5	30.0	40.0
76-77	34.07369596907586	35.0	32.5	37.0	29.0	39.0
78-79	34.67797894351392	35.0	34.0	37.0	30.0	39.0
80-81	34.53836007054376	35.0	34.0	37.0	30.0	39.0
82-83	34.30960062119955	35.0	34.0	36.0	30.0	38.0
84-85	34.10697792164072	35.0	34.0	36.0	30.0	37.0
86-87	33.888110969005766	35.0	34.0	35.5	30.0	37.0
88-89	33.72753874689056	35.0	34.0	35.0	30.0	36.5
90-91	33.56103977524728	35.0	34.0	35.0	30.0	36.0
92-93	33.452069934655114	35.0	33.0	35.0	30.0	36.0
94-95	33.42804672584066	35.0	33.0	35.0	30.0	36.0
96-97	33.44547208163053	35.0	33.0	35.0	30.0	35.0
98-99	33.50986464191927	35.0	33.0	35.0	30.0	35.0
100	32.319273531259284	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	16.0
21	159.0
22	714.0
23	2702.0
24	7149.0
25	15817.0
26	29555.0
27	47825.0
28	69817.0
29	94748.0
30	123601.0
31	156475.0
32	197700.0
33	255920.0
34	354762.0
35	516314.0
36	862576.0
37	1670700.0
38	2224809.0
39	1133949.0
40	53708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06696182719527	19.926072595779882	13.974522644903672	24.032442932121178
2	15.165349323738953	20.833487870989494	40.56406287129004	23.437099933981514
3	18.447626825963656	25.41604308057964	30.228435218624526	25.90789487483217
4	12.873975799019366	17.428599126591536	38.17734494160523	31.520080132783868
5	13.507430980498583	37.608411896014616	35.37534489607524	13.508812227411562
6	31.075625570563687	37.13335248321337	18.27012380832283	13.520898137900112
7	27.425105083474634	32.22580778005229	22.584729906118696	17.764357230354385
8	25.084710082667627	36.25554449136989	20.799007855458136	17.86073757050434
9	25.759973561910936	16.668657537988764	21.04245262387035	36.52891627622994
10-11	24.659810395724683	26.5060579187525	28.496690506817863	20.337441178704957
12-13	25.21995717622997	23.97984684018027	28.718892209281417	22.081303774308346
14-15	22.32446078294702	25.447479237173138	26.273349787224028	25.954710192655806
16-17	22.065074123080866	28.570919739067115	27.036872386341926	22.3271337515101
18-19	22.094213189841465	27.850085807233537	28.585859574129845	21.469841428795153
20-21	22.812537609464727	27.024720920985366	28.514052526697363	21.64868894285254
22-23	22.55808908670985	26.91027312483684	28.354738048318218	22.17689974013509
24-25	22.359703018962644	27.043171096725064	28.417035905720777	22.180089978591514
26-27	22.271390914484982	27.20756551740866	28.38419437496308	22.136849193143274
28-29	22.226714040337107	27.260628583205797	28.37840407552034	22.134253300936756
30-31	22.490040999955905	27.159099416176215	28.26567776577929	22.08518181808859
32-33	22.259340630544227	27.314938065895067	28.205803415648596	22.219917887912114
34-35	22.449576171787992	27.241272182454573	28.27579463122607	22.03335701453136
36-37	22.39893870611746	27.178172534685523	28.191197005810487	22.23169175338653
38-39	22.233487928142733	27.343305615280826	28.296447977689613	22.126758478886824
40-41	22.501317713663923	27.23618458049679	28.11848776763303	22.14400993820625
42-43	22.36003604525589	27.30816642768848	27.93538227876593	22.396415248289703
44-45	22.415217885281553	27.41374387457461	27.848553846439223	22.322484393704617
46-47	22.477122739464033	27.38197665812734	27.622492372575824	22.518408229832808
48-49	22.418775212930534	27.47749341657455	27.616916250794223	22.486815119700697
50-51	22.423340020176692	27.578055949797253	27.51786960444868	22.480734425577374
52-53	22.59980067539392	27.458821995272558	27.39030287912332	22.55107445021021
54-55	22.590300212103006	27.35952362345645	27.457367488684692	22.59280867575585
56-57	22.493447313874604	27.499063107963728	27.453847732568427	22.553641845593244
58-59	22.4649314998614	27.53269308871594	27.458207473899204	22.544167937523458
60-61	22.528614653176444	27.53996155378141	27.4710707932793	22.460352999762847
62-63	22.532885848044845	27.587629168812143	27.429468805625813	22.450016177517202
64-65	22.50643288180157	27.524656143297822	27.37693651825362	22.591974456646994
66-67	22.55333886990218	27.571074122685918	27.25787517354441	22.61771183386749
68-69	22.48386367277979	27.597686829775505	27.337779238264503	22.580670259180202
70-71	22.44906755467733	27.392902433122117	27.416218944639642	22.741811067560906
72-73	22.557207596063318	27.40211039736145	27.324029427763257	22.71665257881197
74-75	22.432836078646183	27.561937687538478	27.281660069998203	22.723566163817132
76-77	22.600892829589192	27.414387900510384	27.25733063449068	22.727388635409742
78-79	22.517914179565203	27.426414098152957	27.227244580508497	22.828427141773343
80-81	22.529977097471658	27.471994757609615	27.24385543691408	22.754172708004642
82-83	22.629319478263547	27.430590662081254	27.315690806217958	22.62439905343724
84-85	22.677290197805814	27.39269454557636	27.256251811075654	22.673763445542164
86-87	22.521911525142407	27.442892342477165	27.354336432057792	22.680859700322635
88-89	22.587946439365915	27.401568838001424	27.324697376904776	22.68578734572789
90-91	22.63779695860873	27.422528947130637	27.34201058523924	22.597663509021395
92-93	22.65554884729083	27.343304695776787	27.378388835060818	22.622757621871568
94-95	22.51750812277715	27.340046361774874	27.45840428541143	22.684041230036545
96-97	22.598187887084464	27.470918113931724	27.533209718095975	22.397684280887834
98-99	23.686640382093767	29.40094252551382	27.724194821353077	19.188222271039336
100	22.617721470797328	30.459584853723875	21.470797328802437	25.451896346676357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1257.0
1	1102.5
2	1543.5
3	2268.5
4	3018.0
5	4822.5
6	6512.5
7	7930.5
8	9114.5
9	9335.0
10	8588.0
11	7728.5
12	8245.0
13	10689.5
14	16438.5
15	24454.5
16	31105.5
17	35368.5
18	37004.5
19	35890.5
20	33240.0
21	30493.0
22	29206.5
23	30551.0
24	35405.5
25	43911.5
26	55299.5
27	68605.0
28	82915.0
29	99546.0
30	116815.5
31	133833.5
32	152856.5
33	171968.5
34	190846.0
35	208273.0
36	225811.0
37	242738.5
38	255953.5
39	268468.0
40	281734.5
41	293008.0
42	300118.0
43	307866.0
44	315555.0
45	321481.0
46	324839.0
47	326259.5
48	327866.5
49	327266.5
50	324356.5
51	316908.5
52	306602.0
53	293737.5
54	277619.0
55	260215.5
56	240591.0
57	218840.0
58	196354.5
59	172135.5
60	145897.5
61	120900.0
62	97997.5
63	76814.5
64	58205.5
65	43531.5
66	32809.0
67	25264.5
68	19356.5
69	14396.5
70	10649.5
71	7756.5
72	5518.5
73	3763.0
74	2473.5
75	1602.5
76	1021.0
77	651.0
78	435.0
79	289.0
80	190.5
81	119.5
82	81.0
83	61.0
84	48.0
85	30.5
86	16.5
87	11.5
88	7.0
89	3.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.43546238903024E-4
20-21	0.0
22-23	6.26731345341506E-4
24-25	0.00685679606993011
26-27	0.028600013960901668
28-29	0.027677417269389794
30-31	0.028725300838736492
32-33	0.04993108316491168
34-35	0.02360052339486353
36-37	0.0188816556221147
38-39	0.014213419987795548
40-41	0.007353836299162943
42-43	0.010937544778860461
44-45	0.0046304978633134
46-47	0.003354584156192111
48-49	0.008316420246102688
50-51	0.0020106997758273537
52-53	0.0018540525415229091
54-55	9.578931532898496E-4
56-57	0.006169142034908173
58-59	0.004218104299845836
60-61	0.007131727891148769
62-63	0.005468257903575435
64-65	0.0012659877711335798
66-67	0.0039005792469136396
68-69	0.011089432937333181
70-71	0.014671744563888697
72-73	0.004841880013566258
74-75	0.0013860688867147722
76-77	0.0025039707462705765
78-79	0.0028737464055900333
80-81	0.0036926817007159095
82-83	0.003095010145284538
84-85	0.004105003251869504
86-87	4.0689153825915685E-5
88-89	2.5582492728382756E-4
90-91	0.003855612993013462
92-93	5.96665475324601E-4
94-95	0.0020669653344727694
96-97	4.4538343148693395E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	98.0
20-21	407.0
22-23	912.0
24-25	2294.0
26-27	2622.0
28-29	6258.0
30-31	13672.0
32-33	18197.0
34-35	25474.0
36-37	37921.0
38-39	43751.0
40-41	46776.0
42-43	57023.0
44-45	64954.0
46-47	64359.0
48-49	59838.0
50-51	53481.0
52-53	51510.0
54-55	57257.0
56-57	60909.0
58-59	61660.0
60-61	61350.0
62-63	61284.0
64-65	65992.0
66-67	70233.0
68-69	72019.0
70-71	70599.0
72-73	69058.0
74-75	71777.0
76-77	72923.0
78-79	77465.0
80-81	77213.0
82-83	76475.0
84-85	78466.0
86-87	84004.0
88-89	90593.0
90-91	98931.0
92-93	105475.0
94-95	124283.0
96-97	296476.0
98-99	531845.0
100-101	4833188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.15558374359796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42079594374964	77.90256457094505
2	6.7477964253219875	11.627206800131589
3	1.5703646180261754	4.058870410690122
4	0.5987425638915651	2.0634006041686517
5	0.26165335983918986	1.1271448977709546
6	0.13845457566652083	0.7157180873112753
7	0.07808944682244837	0.47094893126418835
8	0.052635798066102274	0.36278943265561486
9	0.030341561529642933	0.23526854507508135
>10	0.09933751350969733	1.2658193939329212
>50	0.001378070981222837	0.0752533412027822
>100	3.6448161483291494E-4	0.05844529366605778
>500	2.2820501875758503E-5	0.013585845393556414
>1k	2.282047914027433E-5	0.02298384579215915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.8367969804919334E-5	0.0	0.0	0.0
3	0.0	3.8367969804919334E-5	0.0	0.0	0.0
4	0.0	7.673593960983867E-5	0.0	0.0	0.0
5	0.0	7.673593960983867E-5	0.0	0.0	0.0
6	1.2789323268306445E-5	7.673593960983867E-5	0.0	0.0	1.2789323268306445E-5
7	1.2789323268306445E-5	7.673593960983867E-5	0.0	1.2789323268306445E-5	1.2789323268306445E-5
8	1.2789323268306445E-5	1.0231458614645156E-4	0.0	1.2789323268306445E-5	1.2789323268306445E-5
9	1.2789323268306445E-5	1.9183984902459668E-4	0.0	1.2789323268306445E-5	1.2789323268306445E-5
10-11	1.2789323268306445E-5	1.9183984902459668E-4	0.0	2.557864653661289E-5	1.2789323268306445E-5
12-13	1.2789323268306445E-5	1.9183984902459668E-4	0.0	3.8367969804919334E-5	1.9183984902459667E-5
14-15	1.2789323268306445E-5	2.749704502685886E-4	0.0	5.755195470737901E-5	5.115729307322578E-5
16-17	5.755195470737901E-5	2.813651119027418E-4	0.0	7.034127797568545E-5	6.394661634153223E-5
18-19	7.673593960983867E-5	3.069437584393547E-4	0.0	8.952526287814512E-5	7.034127797568545E-5
20-21	7.673593960983867E-5	3.325224049759676E-4	0.0	3.133384200735079E-4	7.673593960983867E-5
22-23	7.673593960983867E-5	3.900743596833466E-4	0.0	6.970181181227013E-4	1.0231458614645156E-4
24-25	7.673593960983867E-5	4.6041563765903207E-4	0.0	0.0018608465355385879	1.406825559513709E-4
26-27	7.673593960983867E-5	4.923889458297981E-4	0.0	0.0037344823943454824	1.470772175855241E-4
28-29	8.952526287814512E-5	5.051782690981046E-4	0.0	0.011753388083573624	1.662612024879838E-4
30-31	1.0231458614645156E-4	5.371515772688708E-4	0.0	0.012872453869550438	1.7905052575629024E-4
32-33	1.0231458614645156E-4	5.371515772688708E-4	0.0	0.014624591157308419	1.9183984902459668E-4
34-35	1.0231458614645156E-4	6.138875168787093E-4	0.0	0.017016194608481725	1.9183984902459668E-4
36-37	1.0231458614645156E-4	6.90623456488548E-4	0.0	0.021550009707096363	1.9183984902459668E-4
38-39	1.0231458614645156E-4	6.90623456488548E-4	0.0	0.03439049026847603	2.0462917229290312E-4
40-41	1.0231458614645156E-4	7.545700728300803E-4	0.0	0.047819279700197795	2.1741849556120957E-4
42-43	1.0231458614645156E-4	7.929380426349997E-4	0.0	0.05670785937167078	2.238131571953628E-4
44-45	1.0870924778060478E-4	9.080419520497577E-4	0.0	0.06820546098987827	2.30207818829516E-4
46-47	1.15103909414758E-4	9.847778916595962E-4	0.0	0.08015068892247648	2.493918037319757E-4
48-49	1.15103909414758E-4	9.847778916595962E-4	0.0	0.09199999693056242	2.685757886344354E-4
50-51	1.2789323268306446E-4	0.0011318551092451205	0.0	0.10372780636759943	2.685757886344354E-4
52-53	1.2789323268306446E-4	0.00115103909414758	0.0	0.1367754176929033	2.87759773536895E-4
54-55	1.2789323268306446E-4	0.00115103909414758	0.0	0.17607061343477481	3.069437584393547E-4
56-57	1.2789323268306446E-4	0.001176617740684193	0.0	0.2149629454936947	3.069437584393547E-4
58-59	1.2789323268306446E-4	0.0012021963872208058	0.0	0.2401898856404292	3.069437584393547E-4
60-61	1.342878943172177E-4	0.0012405643570257252	0.0	0.2564451155144467	3.069437584393547E-4
62-63	1.406825559513709E-4	0.0012469590186598785	0.0	0.2687548391601916	3.1973308170766114E-4
64-65	1.406825559513709E-4	0.0013684575897087896	0.0	0.2744716666611246	3.325224049759676E-4
66-67	1.406825559513709E-4	0.0013684575897087896	0.0	0.2800286276212038	3.325224049759676E-4
68-69	1.406825559513709E-4	0.0013940362362454026	0.0	0.28578382309194167	3.3891706661012084E-4
70-71	1.470772175855241E-4	0.0014196148827820155	0.0	0.2908547897678252	3.45311728244274E-4
72-73	1.5347187921967734E-4	0.001432404206050322	0.0	0.2951136344161712	3.8367969804919337E-4
74-75	1.5347187921967734E-4	0.0014515881909527815	0.0	0.29856675169861396	3.8367969804919337E-4
76-77	1.5347187921967734E-4	0.0014899561607577007	0.0	0.3020006849961543	3.8367969804919337E-4
78-79	1.5347187921967734E-4	0.0015091401456601605	0.0	0.3044242617554983	3.8367969804919337E-4
80-81	1.5347187921967734E-4	0.0015091401456601605	0.0	0.30528114641447485	3.900743596833466E-4
82-83	1.5347187921967734E-4	0.0015091401456601605	0.0	0.30572877272886556	3.9646902131749984E-4
84-85	1.5347187921967734E-4	0.001521929468928467	0.0	0.3059397965627927	3.9646902131749984E-4
86-87	1.5347187921967734E-4	0.0015347187921967735	0.0	0.3060293218256708	3.9646902131749984E-4
88	1.5347187921967734E-4	0.00154750811546508	0.0	0.30603571648730493	4.220476678541127E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8600	0.0	48.35988	1
GTATCAA	15090	0.0	35.78139	1
TCAACGC	18080	0.0	29.253096	4
ATCAACG	18370	0.0	28.837542	3
CAACGCA	18790	0.0	28.147738	5
TATCAAC	19325	0.0	27.56633	2
AACGCAG	19445	0.0	27.26513	6
ACGCAGA	22715	0.0	23.377506	7
CGCAGAG	23525	0.0	22.51841	8
GTACATG	22700	0.0	21.558947	1
TACATGG	22540	0.0	21.071178	2
TGGTATC	4365	0.0	20.632534	2
ACATGGG	22865	0.0	20.40009	3
GTGGTAT	4760	0.0	20.348331	1
GCAGAGT	27600	0.0	19.178291	9
GAGTACT	15625	0.0	18.134665	12-13
CATGGGG	17185	0.0	17.7738	4
TATACCG	480	0.0	17.700668	5
AGAGTAC	24645	0.0	17.573544	10-11
CAGAGTA	26860	0.0	17.365898	10-11
>>END_MODULE