FastQCFastQC Report
Fri 27 May 2016
SRR2140212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10995510
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132800.051.5016751
GTATCAA241750.037.3565371
TCAACGC290400.030.5098634
ATCAACG295250.029.9942153
CAACGCA300450.029.4608755
TATCAAC303600.029.3944112
AACGCAG310350.028.5348516
ACGCAGA360150.024.6128987
GTGGTAT65450.023.366921
TGGTATC62600.022.8611762
GTACATG359950.022.5971091
CGCAGAG398650.022.2358868
TACATGG361200.021.9038942
ACATGGG366950.021.0018583
GCAGAGT435700.020.25689
GAGTACT238800.019.33835212-13
CATGGGG286850.019.0477564
CAGAGTA419150.018.47296710-11
AGAGTAC388200.017.89891210-11
TATATAC122400.017.6951663
Produced by FastQC (version 0.11.5)