##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10995510
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26000922194605	33.0	31.0	34.0	31.0	34.0
2	32.56613244860857	34.0	31.0	34.0	31.0	34.0
3	32.211589457878716	33.0	31.0	34.0	30.0	34.0
4	36.12496100681096	37.0	35.0	37.0	35.0	37.0
5	35.99167623875564	37.0	35.0	37.0	35.0	37.0
6	36.091333917208026	37.0	35.0	37.0	35.0	37.0
7	36.06724653972394	37.0	35.0	37.0	35.0	37.0
8	36.070774707130454	37.0	36.0	37.0	35.0	37.0
9	37.877661063470455	39.0	38.0	39.0	35.0	39.0
10-11	37.795655135596256	39.0	38.0	39.0	35.0	39.0
12-13	37.75529529780792	39.0	38.0	39.0	35.0	39.0
14-15	39.09699149925743	40.0	38.5	41.0	36.0	41.0
16-17	39.07840395761542	40.0	38.0	41.0	36.0	41.0
18-19	39.04925469578036	40.0	38.5	41.0	36.0	41.0
20-21	39.03700654588317	40.0	39.0	41.0	35.5	41.0
22-23	38.963279657431706	40.0	38.0	41.0	35.0	41.0
24-25	38.896653115221476	40.0	38.0	41.0	35.0	41.0
26-27	38.72562263855413	40.0	38.0	41.0	35.0	41.0
28-29	38.589201378733435	40.0	38.0	41.0	34.0	41.0
30-31	38.44063344108696	40.0	38.0	41.0	34.0	41.0
32-33	38.26109623140301	40.0	38.0	41.0	34.0	41.0
34-35	38.36613788609282	40.0	38.0	41.0	34.0	41.0
36-37	38.352516541711864	40.0	38.0	41.0	34.0	41.0
38-39	38.297008470798815	40.0	38.0	41.0	34.0	41.0
40-41	38.20586271174113	40.0	38.0	41.0	33.5	41.0
42-43	38.16710717127408	40.0	38.0	41.0	33.0	41.0
44-45	38.209365911183816	40.0	38.0	41.0	33.0	41.0
46-47	38.143984988336214	40.0	37.5	41.0	33.0	41.0
48-49	38.040373188373565	40.0	37.0	41.0	33.0	41.0
50-51	37.92538273043749	40.0	37.0	41.0	33.0	41.0
52-53	37.701065174532246	40.0	36.5	41.0	33.0	41.0
54-55	37.57625862597105	39.0	36.0	41.0	32.5	41.0
56-57	37.41885866217823	39.0	36.0	41.0	32.0	41.0
58-59	37.20051079006383	39.0	35.0	41.0	32.0	41.0
60-61	36.98501934584837	39.0	35.0	40.0	31.5	41.0
62-63	36.680078003668974	38.0	35.0	40.0	31.0	41.0
64-65	36.3401808250415	37.5	35.0	40.0	31.0	41.0
66-67	36.03611023567625	37.0	35.0	40.0	31.0	41.0
68-69	35.73944981818126	36.5	34.0	39.0	30.0	41.0
70-71	35.42593341915459	36.0	34.0	39.0	30.0	40.5
72-73	35.095721972477875	35.5	34.0	39.0	30.0	40.0
74-75	34.760711263996164	35.0	34.0	37.5	29.5	40.0
76-77	33.62544454804002	34.5	32.5	36.0	28.5	39.0
78-79	34.286866118675185	35.0	33.5	37.0	29.5	39.0
80-81	34.18015845693189	35.0	34.0	36.0	30.0	39.0
82-83	33.97096294019443	35.0	34.0	36.0	30.0	37.0
84-85	33.78977948537179	35.0	34.0	35.5	29.5	37.0
86-87	33.59072600640019	35.0	33.0	35.0	29.0	37.0
88-89	33.44556314165034	35.0	33.0	35.0	29.0	36.0
90-91	33.29140570596829	35.0	33.0	35.0	29.0	36.0
92-93	33.18706622812768	35.0	33.0	35.0	29.0	36.0
94-95	33.175194749094146	35.0	33.0	35.0	29.0	35.0
96-97	33.219358311694094	35.0	33.0	35.0	29.0	35.0
98-99	33.30312877736796	35.0	33.0	35.0	29.5	35.0
100	32.04381728450131	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	11.0
20	45.0
21	305.0
22	1425.0
23	4872.0
24	13103.0
25	28323.0
26	52194.0
27	83044.0
28	119335.0
29	159547.0
30	205132.0
31	255397.0
32	320298.0
33	411822.0
34	568909.0
35	831988.0
36	1398713.0
37	2478415.0
38	2786198.0
39	1230446.0
40	45987.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.41306678817081	19.70350624936906	13.952549722568575	23.930877239891558
2	15.513850653584962	20.803664404834336	40.11369186149619	23.568793080084507
3	18.80341157436081	25.14155323400188	29.917047958666764	26.13798723297055
4	13.182353524302194	17.3278729226748	37.31888743678101	32.170886116241995
5	13.980934035801887	37.174764972247765	34.88799519076423	13.956305801186119
6	31.594705475234893	36.4986344426043	17.966697315540618	13.939962766620193
7	27.842537544870588	31.93158843928112	22.111734698981675	18.114139316866613
8	25.592155343408358	35.849896912467	20.256477416690995	18.301470327433652
9	25.96448004685549	16.46032789747815	20.769377682344885	36.805814373321475
10-11	25.204674453481466	26.300380791795924	27.709187659326396	20.785757095396214
12-13	25.674584444013966	23.810077931810348	28.048253332496625	22.467084291679058
14-15	22.707327809260324	25.048633487669058	25.83667788033479	26.407360822735825
16-17	22.45712568130082	28.270457668630193	26.42655956840565	22.845857081663333
18-19	22.5346956898021	27.36119313993105	28.078102794808547	22.026008375458304
20-21	23.35785844707163	26.485767691205663	28.089805990364457	22.06656787135825
22-23	23.135771110357584	26.381784653468948	27.926222896677384	22.556221339496084
24-25	22.85423528134305	26.514623799417063	28.122004641138748	22.50913627810113
26-27	22.71024593547722	26.607018194486283	28.103523407196178	22.57921246284032
28-29	22.80899593201455	26.674139917079344	27.98925804493449	22.52760610597162
30-31	23.08835059403717	26.383695625865727	27.92631296296946	22.60164081712764
32-33	22.876966835760644	26.54981842274462	27.826400991793914	22.74681374970082
34-35	23.00167769594288	26.650580567182132	27.83425749770284	22.513484239172143
36-37	23.098262321649717	26.507077492622606	27.68637723220937	22.70828295351831
38-39	22.789159052831405	26.820438136107853	27.773153176346344	22.617249634714394
40-41	23.066981175588737	26.608075975367434	27.641690680923254	22.68325216812058
42-43	22.888079824570607	26.74675703938418	27.4329554979651	22.932207638080115
44-45	22.854220999795807	26.821620394228763	27.35021271976396	22.97394588621147
46-47	23.026067869464917	26.782152777406136	27.164195984201108	23.027583368927836
48-49	23.08736653318533	27.0212124708764	26.973901462005657	22.917519533932616
50-51	23.020653788175615	27.0524049217325	26.96564139228656	22.961299897805322
52-53	23.29686553805448	26.884668277955566	26.84975271572207	22.968713468267886
54-55	23.250430357855397	26.868428526678095	26.81340451416199	23.067736601304514
56-57	23.238031410527864	27.08833121597653	26.704035252456443	22.969602121039163
58-59	23.090403362882206	27.06053751072079	26.87989536046019	22.96916376593682
60-61	23.141419848935982	27.051387237772662	26.785646139570673	23.02154677372069
62-63	23.151619957730396	27.011665626604408	26.893973488720814	22.942740926944385
64-65	23.207341913372627	26.957915731682103	26.8073616262561	23.02738072868917
66-67	23.221922001168032	27.065488359155054	26.582995718971603	23.12959392070531
68-69	23.179057124098534	27.028111816812622	26.698167004847008	23.094664054241836
70-71	23.15302822619042	26.88250823180358	26.694290820306954	23.27017272169905
72-73	23.193618442293914	26.893952254832097	26.613952727218283	23.298476575655705
74-75	23.017829772852004	26.974955985137488	26.669902693451895	23.33731154855861
76-77	23.164563764223256	26.98654903760362	26.613485862097658	23.235401336075462
78-79	23.03959452354505	26.956517956806664	26.686724093215652	23.317163426432632
80-81	23.07084833076842	26.920025586038665	26.743483459510557	23.265642623682357
82-83	23.302136171082484	26.782840232338966	26.847689913105548	23.067333683473
84-85	23.231603595287968	26.817412254869353	26.77591845790193	23.17506569194075
86-87	23.09232158299767	26.936752806307517	26.759191662949526	23.21173394774528
88-89	23.232819958673957	26.78197157156972	26.69914015914923	23.286068310607096
90-91	23.205125025695768	26.79248904381896	26.779666136629665	23.222719793855607
92-93	23.268427370001056	26.756435905894264	26.873596396636383	23.101540327468296
94-95	22.92327433458994	26.956488198240457	26.974336137696152	23.14590132947345
96-97	23.014958651162704	27.02170759218329	26.99384349938973	22.969490257264276
98-99	24.236892863633802	29.023775720576122	27.150181400013324	19.58915001577675
100	23.139475345096255	30.12728407509883	20.84372334382114	25.889517235983778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2387.0
1	2033.5
2	2553.0
3	3633.5
4	4703.0
5	7380.5
6	9970.0
7	12516.0
8	14763.5
9	15087.5
10	13607.0
11	11800.0
12	12342.5
13	16243.5
14	25712.0
15	39147.0
16	50178.5
17	56734.5
18	58533.5
19	55874.5
20	50625.0
21	44808.0
22	41532.5
23	42518.5
24	48065.5
25	59062.0
26	74338.0
27	90750.0
28	108647.0
29	128831.0
30	147812.5
31	166217.0
32	186520.0
33	207752.5
34	228123.5
35	246843.0
36	269359.0
37	291447.0
38	308238.5
39	324946.5
40	344297.5
41	360992.5
42	371928.0
43	383218.0
44	396863.0
45	416459.5
46	435974.5
47	454783.0
48	469084.0
49	472857.5
50	473116.5
51	475099.5
52	459414.5
53	430494.0
54	413237.0
55	396639.0
56	375693.5
57	347843.0
58	314429.5
59	277980.0
60	238451.5
61	204498.0
62	170725.5
63	139647.0
64	114287.5
65	88506.5
66	69039.0
67	51575.5
68	34525.0
69	24665.5
70	18144.5
71	12265.0
72	8518.0
73	5925.0
74	4268.5
75	2936.5
76	1829.5
77	1042.5
78	704.0
79	502.5
80	324.5
81	196.0
82	132.5
83	96.0
84	67.0
85	39.0
86	24.5
87	16.0
88	10.5
89	7.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.72920310199345E-4
20-21	0.0
22-23	5.457381308323489E-4
24-25	0.006713838508439809
26-27	0.028384271528000236
28-29	0.02688135575628384
30-31	0.028138371707389004
32-33	0.04907330376671726
34-35	0.023342534769602272
36-37	0.018664690578127544
38-39	0.014581164354393125
40-41	0.007258797595474626
42-43	0.010225340772420283
44-45	0.004673599125064667
46-47	0.003352930136538608
48-49	0.007857497644779526
50-51	0.0020051912815437145
52-53	0.0018878153619764945
54-55	0.0010672332066834528
56-57	0.006347985052336264
58-59	0.004309653822479867
60-61	0.007392374730548689
62-63	0.0057019984750422414
64-65	0.0011458489239768786
66-67	0.004052589719961445
68-69	0.01068558076467318
70-71	0.014030938218772394
72-73	0.004507499767903302
74-75	0.0015013916622929318
76-77	0.0023667547978019517
78-79	0.002414009869385755
80-81	0.0032949630576333045
82-83	0.0029721655030879907
84-85	0.003762293137943408
86-87	1.7114324141648187E-5
88-89	1.793260130140937E-4
90-91	0.0037080451004060455
92-93	6.037931481194862E-4
94-95	0.002096129217104268
96-97	3.691008996114994E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	231.0
20-21	670.0
22-23	1549.0
24-25	3606.0
26-27	4163.0
28-29	8639.0
30-31	17358.0
32-33	23452.0
34-35	32670.0
36-37	47065.0
38-39	54795.0
40-41	59580.0
42-43	75840.0
44-45	85990.0
46-47	86044.0
48-49	77906.0
50-51	69643.0
52-53	67981.0
54-55	74122.0
56-57	79201.0
58-59	80762.0
60-61	80452.0
62-63	80623.0
64-65	88243.0
66-67	94083.0
68-69	97887.0
70-71	95809.0
72-73	92377.0
74-75	96168.0
76-77	96146.0
78-79	105983.0
80-81	106914.0
82-83	105232.0
84-85	110014.0
86-87	119671.0
88-89	129566.0
90-91	144295.0
92-93	152853.0
94-95	183657.0
96-97	439806.0
98-99	773332.0
100-101	6851132.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.68128352154145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.45837809090979	74.85991900402674
2	7.229273672079292	12.099097996161646
3	1.7688922527282096	4.440695223588223
4	0.68131542661682	2.2805339752928835
5	0.31020017391691296	1.297897435098633
6	0.168897248828615	0.8480123139141401
7	0.09687933213476232	0.567489080182264
8	0.06350100890845013	0.4251076744297556
9	0.04594222020392952	0.3460053559044704
>10	0.1696753635480532	2.3132482967740446
>50	0.005454669052315398	0.3003881113424217
>100	0.0015282699735269594	0.19254608534286108
>500	6.227109932874448E-5	0.029059447941919475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.6378485399949615E-5	0.0	1.8189242699974807E-5	0.0
3	0.0	3.6378485399949615E-5	0.0	1.8189242699974807E-5	0.0
4	0.0	7.275697079989923E-5	0.0	2.728386404996221E-5	0.0
5	0.0	7.275697079989923E-5	0.0	2.728386404996221E-5	0.0
6	0.0	7.275697079989923E-5	0.0	3.6378485399949615E-5	0.0
7	0.0	7.275697079989923E-5	0.0	3.6378485399949615E-5	0.0
8	0.0	8.185159214988663E-5	0.0	3.6378485399949615E-5	0.0
9	0.0	1.7279780564976067E-4	0.0	6.366234944991183E-5	9.094621349987404E-6
10-11	0.0	1.7279780564976067E-4	0.0	7.275697079989923E-5	9.094621349987404E-6
12-13	0.0	1.8189242699974807E-4	0.0	7.275697079989923E-5	9.094621349987404E-6
14-15	0.0	2.4100746577466618E-4	0.0	7.275697079989923E-5	1.8189242699974807E-5
16-17	0.0	2.546493977996473E-4	0.0	7.730428147489293E-5	3.6378485399949615E-5
18-19	0.0	2.910278831995969E-4	0.0	9.094621349987404E-5	5.9115038774918124E-5
20-21	9.094621349987404E-6	3.5469023264950877E-4	0.0	1.5460856294978586E-4	6.366234944991183E-5
22-23	9.094621349987404E-6	4.0925796074943314E-4	0.0	3.4104830062452765E-4	7.275697079989923E-5
24-25	9.094621349987404E-6	4.4108913547438906E-4	0.0	9.91313727148627E-4	8.185159214988663E-5
26-27	9.094621349987404E-6	4.547310674993702E-4	0.0	0.002596514395421404	9.094621349987404E-5
28-29	9.094621349987404E-6	4.592783781743639E-4	0.0	0.00966758249503661	1.0004083484986144E-4
30-31	9.094621349987404E-6	5.411299703242505E-4	0.0	0.010363321028310647	1.1368276687484255E-4
32-33	9.094621349987404E-6	5.456772809992442E-4	0.0	0.012013994803333362	1.1823007754983625E-4
34-35	9.094621349987404E-6	6.548127371990931E-4	0.0	0.0141512308205804	1.1823007754983625E-4
36-37	9.094621349987404E-6	7.730428147489294E-4	0.0	0.01808010724377496	1.1823007754983625E-4
38-39	9.094621349987404E-6	8.003266787988915E-4	0.0	0.02738390488481207	1.2277738822482996E-4
40-41	9.094621349987404E-6	8.821782709487782E-4	0.0	0.03737434643777324	1.2732469889982365E-4
42-43	9.094621349987404E-6	9.094621349987405E-4	0.0	0.04439084680928852	1.3187200957481734E-4
44-45	9.094621349987404E-6	0.0010140502805235955	0.0	0.05355822513007582	1.4551394159979846E-4
46-47	9.094621349987404E-6	0.0010913545619984884	0.0	0.06355776130438698	1.5006125227479215E-4
48-49	9.094621349987404E-6	0.0010913545619984884	0.0	0.07339814160507334	1.5460856294978586E-4
50-51	9.094621349987404E-6	0.001282341610348224	0.0	0.08351590785693433	1.5460856294978586E-4
52-53	9.094621349987404E-6	0.0013096254743981861	0.0	0.10438806385515542	1.6825049497476695E-4
54-55	9.094621349987404E-6	0.0013278147170981609	0.0	0.12918909627657107	1.8189242699974807E-4
56-57	9.094621349987404E-6	0.0013460039597981358	0.0	0.15645022377315831	1.8189242699974807E-4
58-59	9.094621349987404E-6	0.001359645891823117	0.0	0.18104662721419926	1.8189242699974807E-4
60-61	9.094621349987404E-6	0.0013914770665480727	0.0	0.1930106016001077	1.8189242699974807E-4
62-63	9.094621349987404E-6	0.001405118998573054	0.0	0.20643881002336406	1.8189242699974807E-4
64-65	9.094621349987404E-6	0.0016097479789477704	0.0	0.21307788360885488	1.8189242699974807E-4
66-67	9.094621349987404E-6	0.0016415791536727265	0.0	0.21861650801099722	1.8189242699974807E-4
68-69	9.094621349987404E-6	0.0016734103283976825	0.0	0.22430519366541435	1.8189242699974807E-4
70-71	9.094621349987404E-6	0.0017052415031226383	0.0	0.22875701081623318	1.8189242699974807E-4
72-73	9.094621349987404E-6	0.0017279780564976067	0.0	0.23266315068605276	1.9098704834973548E-4
74-75	9.094621349987404E-6	0.0017779984739225373	0.0	0.2357234907703235	1.9098704834973548E-4
76-77	9.094621349987404E-6	0.001878039308772399	0.0	0.23879747278661928	1.955343590247292E-4
78-79	9.094621349987404E-6	0.0019189651048473423	0.0	0.24091651956116633	2.0008166969972288E-4
80-81	9.094621349987404E-6	0.001923512415522336	0.0	0.24168501506524026	2.13723601724704E-4
82-83	9.094621349987404E-6	0.0019326070368723234	0.0	0.24226252352096445	2.273655337496851E-4
84-85	9.094621349987404E-6	0.0019598909009222857	0.0	0.24241258477323924	2.273655337496851E-4
86-87	9.094621349987404E-6	0.002028100561047191	0.0	0.24249443636538914	2.273655337496851E-4
88	9.094621349987404E-6	0.0021918037453469643	0.0	0.24251717291876412	2.273655337496851E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13280	0.0	51.501675	1
GTATCAA	24175	0.0	37.356537	1
TCAACGC	29040	0.0	30.509863	4
ATCAACG	29525	0.0	29.994215	3
CAACGCA	30045	0.0	29.460875	5
TATCAAC	30360	0.0	29.394411	2
AACGCAG	31035	0.0	28.534851	6
ACGCAGA	36015	0.0	24.612898	7
GTGGTAT	6545	0.0	23.36692	1
TGGTATC	6260	0.0	22.861176	2
GTACATG	35995	0.0	22.597109	1
CGCAGAG	39865	0.0	22.235886	8
TACATGG	36120	0.0	21.903894	2
ACATGGG	36695	0.0	21.001858	3
GCAGAGT	43570	0.0	20.2568	9
GAGTACT	23880	0.0	19.338352	12-13
CATGGGG	28685	0.0	19.047756	4
CAGAGTA	41915	0.0	18.472967	10-11
AGAGTAC	38820	0.0	17.898912	10-11
TATATAC	12240	0.0	17.695166	3
>>END_MODULE