FastQCFastQC Report
Fri 27 May 2016
SRR2140211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8218688
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85400.044.086121
GTATCAA152200.035.612351
TCAACGC177800.029.4764694
ATCAACG178100.029.426823
CAACGCA183200.028.630385
TATCAAC189400.028.177472
AACGCAG188250.027.8844916
CTTATAC59200.026.1995121
ACGCAGA221300.023.6824077
CGCAGAG226950.023.1111978
GTACATG210200.020.9064311
TATACAC89150.020.7650813
TACATGG209600.020.6678942
GTGGTAT50200.020.5147041
ACATGGG210650.019.9117093
TGGTATC48050.019.1765672
GCAGAGT272550.019.0303259
TATGCCG45750.018.16129742-43
GAGTACT157600.017.81787912-13
CATGGGG157700.017.7668134
Produced by FastQC (version 0.11.5)