##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8218688
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.330722129858195	33.0	31.0	34.0	31.0	34.0
2	32.62619410786734	34.0	31.0	34.0	31.0	34.0
3	32.29111483487389	33.0	31.0	34.0	30.0	34.0
4	36.16678525818233	37.0	35.0	37.0	35.0	37.0
5	36.04812069761013	37.0	35.0	37.0	35.0	37.0
6	36.14046828398888	37.0	36.0	37.0	35.0	37.0
7	36.12115400900971	37.0	36.0	37.0	35.0	37.0
8	36.12368726005903	37.0	36.0	37.0	35.0	37.0
9	37.941425322387225	39.0	38.0	39.0	35.0	39.0
10-11	37.86859727489351	39.0	38.0	39.0	35.0	39.0
12-13	37.83301799508632	39.0	38.0	39.0	35.0	39.0
14-15	39.21172521696894	41.0	39.0	41.0	36.0	41.0
16-17	39.197390203886556	40.0	39.0	41.0	36.0	41.0
18-19	39.16718994564583	40.0	39.0	41.0	36.0	41.0
20-21	39.157821967333504	40.0	39.0	41.0	36.0	41.0
22-23	39.09223773305746	40.0	39.0	41.0	36.0	41.0
24-25	39.034907951035976	40.0	39.0	41.0	35.0	41.0
26-27	38.87760241988315	40.0	38.0	41.0	35.0	41.0
28-29	38.75380418703879	40.0	38.0	41.0	35.0	41.0
30-31	38.619253073884934	40.0	38.0	41.0	34.5	41.0
32-33	38.45803019888875	40.0	38.0	41.0	34.0	41.0
34-35	38.554743851957255	40.0	38.0	41.0	34.0	41.0
36-37	38.54482014545644	40.0	38.0	41.0	34.0	41.0
38-39	38.4950540312692	40.0	38.0	41.0	34.0	41.0
40-41	38.43346937826695	40.0	38.0	41.0	34.0	41.0
42-43	38.405963370476286	40.0	38.0	41.0	34.0	41.0
44-45	38.45741975685422	40.0	38.0	41.0	34.0	41.0
46-47	38.4103661861109	40.0	38.0	41.0	34.0	41.0
48-49	38.32980339295642	40.0	38.0	41.0	34.0	41.0
50-51	38.23499573236391	40.0	38.0	41.0	33.0	41.0
52-53	38.04222193920572	40.0	37.0	41.0	33.0	41.0
54-55	37.941554079819795	40.0	37.0	41.0	33.0	41.0
56-57	37.801611175780565	40.0	37.0	41.0	33.0	41.0
58-59	37.61506298169613	39.0	36.0	41.0	33.0	41.0
60-61	37.422328037203684	39.0	36.0	41.0	32.0	41.0
62-63	37.14508986038837	39.0	35.0	40.5	32.0	41.0
64-65	36.83711173722902	38.0	35.0	40.0	31.0	41.0
66-67	36.5570254183052	38.0	35.0	40.0	31.0	41.0
68-69	36.268722036314486	37.0	35.0	40.0	31.0	41.0
70-71	35.96056723255266	37.0	35.0	39.0	31.0	41.0
72-73	35.62074931551458	36.0	34.0	39.0	30.5	40.5
74-75	35.276432788583406	36.0	34.0	39.0	30.0	40.0
76-77	34.09628429056514	35.0	32.5	37.0	28.5	39.0
78-79	34.70953467707987	35.0	34.0	37.0	30.0	39.0
80-81	34.56640605395735	35.0	34.0	37.0	30.0	39.0
82-83	34.33174630218453	35.0	34.0	36.0	30.0	38.5
84-85	34.122082397703494	35.0	34.0	36.0	30.0	37.0
86-87	33.89136138999366	35.0	34.0	35.5	30.0	37.0
88-89	33.72254285691906	35.0	34.0	35.0	30.0	36.5
90-91	33.548665262309456	35.0	33.5	35.0	30.0	36.0
92-93	33.430553675307344	35.0	33.0	35.0	29.0	36.0
94-95	33.40213718115366	35.0	33.0	35.0	30.0	36.0
96-97	33.419455863887535	35.0	33.0	35.0	30.0	35.5
98-99	33.48423312005803	35.0	33.0	35.0	30.0	35.0
100	32.303833822456006	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	27.0
21	181.0
22	814.0
23	2934.0
24	7751.0
25	17281.0
26	31673.0
27	51082.0
28	75089.0
29	102327.0
30	132956.0
31	168301.0
32	213455.0
33	273942.0
34	376052.0
35	544429.0
36	897056.0
37	1695234.0
38	2298994.0
39	1273540.0
40	55567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.954567444341485	20.07597806365201	13.727787208858642	24.24166728314787
2	15.03214381662864	21.0599550682542	40.73041828574098	23.177482829376174
3	18.37714243441289	25.549442928116996	30.42523332191221	25.64818131555791
4	12.620092160695235	17.692495444528372	38.42629139833511	31.26112099644128
5	13.3182084537094	37.649074888838705	35.68575665605021	13.346960001401683
6	30.630558064742207	37.60490725526994	18.40674569955691	13.35778898043094
7	26.968805726656125	32.316058232165524	22.719356179477796	17.99577986170055
8	24.629709754160274	36.686646335765516	21.038370114548695	17.645273795525515
9	25.3191385291667	16.744144564193213	21.18570506630742	36.751011840332666
10-11	24.303149115771276	26.75389307879798	28.746437387573298	20.19652041785745
12-13	24.939886998995462	24.050925159830864	29.026816688989776	21.9823711521839
14-15	21.98074072163343	25.641544003130427	26.40278472671064	25.974930548525503
16-17	21.853037857137295	28.94429135161233	27.096180071563737	22.106490719686644
18-19	21.872716700311717	28.066616350168754	28.695041261677872	21.365625687841654
20-21	22.485683551660678	27.263864157047486	28.63769078077733	21.612761510514506
22-23	22.212091663968224	27.279950584779627	28.472052619032233	22.03590513221991
24-25	22.06317457388511	27.38455637969157	28.621857891837404	21.930411154585915
26-27	22.08026535782075	27.536725736492233	28.524449490729104	21.858559414957906
28-29	21.938034118375057	27.54491933820431	28.597037134985154	21.92000940843548
30-31	22.277957101938714	27.479573790532424	28.434141779360928	21.808327328167934
32-33	22.056100334088153	27.487881551198186	28.398374789683295	22.05764332503037
34-35	22.04604742291202	27.651896791123526	28.515153853962676	21.786901932001776
36-37	22.1698102715258	27.554022418636425	28.344635992504386	21.93153131733339
38-39	21.905571596380952	27.600762038838724	28.528346430019656	21.965319934760668
40-41	22.184515004410652	27.474949010176115	28.381372676260785	21.959163309152448
42-43	22.128960051279485	27.57177449440999	28.21684710973341	22.08241834457711
44-45	22.10366444675533	27.747522544914847	28.079715916862792	22.06909709146703
46-47	22.279745554131846	27.615395403331476	27.81922042315184	22.285638619384834
48-49	22.19686851279862	27.71156516289153	27.813921691108767	22.277644633201078
50-51	22.11709417001974	27.77681017963443	27.8833120230575	22.22278362728833
52-53	22.273471894986535	27.74391636039184	27.84758595887546	22.135025785746162
54-55	22.200974819102125	27.69559633047277	27.792255977623682	22.31117287280142
56-57	22.289655496839504	27.83292491324472	27.640006243225855	22.237413346689923
58-59	22.170262570827454	27.96663381208429	27.780476162705675	22.082627454382582
60-61	22.301890789873934	28.055166931218146	27.591184875458108	22.051757403449816
62-63	22.20590059970565	28.19583085300082	27.53351806856919	22.064750478724342
64-65	22.17239539360277	28.088816298390608	27.565528291832514	22.173260016174112
66-67	22.210378122215676	28.071293612523064	27.452787860197343	22.26554040506392
68-69	22.21554827846592	27.973767942743414	27.607686932174673	22.20299684661599
70-71	22.218472517683768	27.74606964975443	27.66515531631837	22.37030251624343
72-73	22.28991779362885	27.79694020008211	27.577769221932268	22.335372784356775
74-75	22.144622491668834	27.90891117266624	27.572942055095723	22.3735242805692
76-77	22.282239680697685	27.840975133740827	27.524576808045946	22.35220837751554
78-79	22.19338142410948	27.84914228640597	27.53668312297198	22.420793166512574
80-81	22.178536816234207	27.913841285999425	27.559261493632643	22.34836040413373
82-83	22.252357366208837	27.912997161154767	27.61722979409929	22.217415678537105
84-85	22.331380287080794	27.86608332237947	27.551287530761552	22.25124885977819
86-87	22.21028788407806	27.877498972219055	27.624941268223346	22.28727187547954
88-89	22.222929071359275	27.84237971092861	27.61080777767855	22.32388344003357
90-91	22.219576862365532	27.936368756835762	27.607763737502744	22.236290643295963
92-93	22.244427081681742	27.88076228789997	27.628339606329966	22.246471024088326
94-95	22.08699758022267	27.9770377191719	27.713977489511414	22.221987211094017
96-97	22.173958863927776	28.09498006428609	27.74110796901621	21.98995310276992
98-99	23.23434573630165	30.036113593667352	27.981315391091403	18.748225278939593
100	22.23141011194056	31.14952726745141	21.715212822363494	24.903849798244536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2351.0
1	1889.5
2	2103.5
3	2953.5
4	3764.5
5	5839.5
6	7871.5
7	9527.5
8	10905.5
9	10829.5
10	9546.0
11	8523.0
12	9143.0
13	11928.5
14	18143.0
15	26589.5
16	33582.0
17	37944.0
18	39527.5
19	38479.5
20	36553.0
21	35268.5
22	35614.0
23	38808.5
24	45951.5
25	57104.0
26	71637.0
27	87734.5
28	104493.0
29	123024.0
30	140706.5
31	157834.5
32	175499.5
33	191722.5
34	208674.0
35	225826.0
36	244047.5
37	261802.0
38	276547.0
39	289449.5
40	303493.5
41	316625.0
42	325674.0
43	333656.5
44	342925.5
45	355125.0
46	363169.5
47	365001.0
48	363436.5
49	359728.0
50	354488.0
51	343729.0
52	327033.0
53	306971.5
54	287591.0
55	267539.0
56	245110.5
57	221858.5
58	197310.5
59	172252.0
60	146378.5
61	120794.5
62	97647.5
63	77118.0
64	59058.5
65	45006.5
66	34793.0
67	26675.5
68	20101.0
69	15058.0
70	11050.5
71	7782.5
72	5587.5
73	3983.0
74	2710.0
75	1790.0
76	1194.5
77	792.5
78	526.0
79	347.0
80	235.5
81	154.5
82	113.5
83	81.5
84	57.5
85	40.5
86	32.0
87	29.5
88	25.5
89	19.0
90	11.5
91	5.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.475326475466644E-4
20-21	0.0
22-23	6.875346619382941E-4
24-25	0.006389763136957453
26-27	0.029141066013919772
28-29	0.027809303110438572
30-31	0.029375274439752377
32-33	0.051125223076165634
34-35	0.02390434575599567
36-37	0.019897930596685254
38-39	0.01499866170098272
40-41	0.008017582678103662
42-43	0.010747521467651639
44-45	0.0047341536043478466
46-47	0.0036611995771637915
48-49	0.008624879658734633
50-51	0.002047435379141772
52-53	0.0019864278980333233
54-55	0.0010227717437235459
56-57	0.00698099449738573
58-59	0.004718802635770667
60-61	0.0077047891303333805
62-63	0.005926494910999476
64-65	0.0013323514564550338
66-67	0.0040644240603582025
68-69	0.011331280354882352
70-71	0.0150104313322679
72-73	0.005127802119377757
74-75	0.0014099449416500286
76-77	0.0024793234282719603
78-79	0.0028450582067467757
80-81	0.0035234766376812856
82-83	0.0033063529175972823
84-85	0.0041800425378929595
86-87	2.4140474386071525E-5
88-89	2.202102077707778E-4
90-91	0.003708517172586773
92-93	6.39253620930173E-4
94-95	0.0021236182138623973
96-97	4.2154635850691086E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	165.0
20-21	525.0
22-23	1205.0
24-25	2485.0
26-27	4512.0
28-29	10485.0
30-31	22582.0
32-33	29275.0
34-35	39759.0
36-37	59896.0
38-39	67686.0
40-41	66459.0
42-43	77344.0
44-45	84981.0
46-47	84728.0
48-49	78199.0
50-51	70566.0
52-53	68880.0
54-55	74174.0
56-57	76523.0
58-59	75675.0
60-61	75570.0
62-63	72984.0
64-65	77949.0
66-67	82598.0
68-69	82192.0
70-71	75780.0
72-73	70913.0
74-75	72298.0
76-77	72963.0
78-79	77544.0
80-81	76609.0
82-83	74510.0
84-85	76882.0
86-87	81805.0
88-89	88061.0
90-91	94935.0
92-93	101143.0
94-95	125433.0
96-97	296971.0
98-99	538018.0
100-101	4907426.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.88499714314294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.78885433945086	78.88189349912409
2	6.552493772424545	11.386268053951369
3	1.5271443475614186	3.980577968251222
4	0.548679344613632	1.9068801315702784
5	0.23643545945974553	1.0271347109848838
6	0.11398615378113272	0.594221198737894
7	0.06941077194263565	0.42215283053574704
8	0.04421057352513752	0.3072988443542627
9	0.031722729664873224	0.24806063486746852
>10	0.08553391286112967	1.115292041077003
>50	0.001211371580100809	0.06867037589244689
>100	2.7532899952043867E-4	0.038781712469151394
>500	4.189413516800665E-5	0.022767998184156132
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.650217650311096E-5	0.0	0.0	0.0
3	0.0	3.650217650311096E-5	0.0	0.0	0.0
4	0.0	8.517174517392556E-5	0.0	1.2167392167703653E-5	1.2167392167703653E-5
5	0.0	8.517174517392556E-5	0.0	1.2167392167703653E-5	1.2167392167703653E-5
6	2.4334784335407306E-5	8.517174517392556E-5	0.0	1.2167392167703653E-5	1.2167392167703653E-5
7	2.4334784335407306E-5	8.517174517392556E-5	0.0	1.2167392167703653E-5	1.2167392167703653E-5
8	2.4334784335407306E-5	8.517174517392556E-5	0.0	1.2167392167703653E-5	2.4334784335407306E-5
9	2.4334784335407306E-5	1.9467827468325845E-4	0.0	2.4334784335407306E-5	2.4334784335407306E-5
10-11	3.650217650311096E-5	1.9467827468325845E-4	0.0	3.0418480419259132E-5	2.4334784335407306E-5
12-13	4.866956867081461E-5	1.9467827468325845E-4	0.0	3.650217650311096E-5	2.4334784335407306E-5
14-15	5.475326475466644E-5	2.859337159410359E-4	0.0	3.650217650311096E-5	2.4334784335407306E-5
16-17	8.517174517392556E-5	2.9201741202488767E-4	0.0	6.0836960838518265E-5	2.4334784335407306E-5
18-19	9.733913734162922E-5	3.2243589244414683E-4	0.0	7.908804909007375E-5	2.4334784335407306E-5
20-21	9.733913734162922E-5	3.711054611149614E-4	0.0	1.0950652950933288E-4	2.4334784335407306E-5
22-23	1.2167392167703653E-4	4.015239415342206E-4	0.0	1.3992500992859202E-4	2.4334784335407306E-5
24-25	1.2775761776088836E-4	4.319424219534797E-4	0.0	1.885945785994066E-4	3.0418480419259132E-5
26-27	1.581760981801475E-4	4.380261180373315E-4	0.0	2.494315394379249E-4	3.650217650311096E-5
28-29	1.581760981801475E-4	4.380261180373315E-4	0.0	5.475326475466644E-4	3.650217650311096E-5
30-31	1.581760981801475E-4	4.988630788758498E-4	0.0	0.0015330914131306602	3.650217650311096E-5
32-33	1.581760981801475E-4	4.988630788758498E-4	0.0	0.0034190371991247265	3.650217650311096E-5
34-35	1.642597942639993E-4	5.962022162174789E-4	0.0	0.006101947172103382	3.650217650311096E-5
36-37	2.068456668509621E-4	6.935413535591082E-4	0.0	0.010196274636535661	3.650217650311096E-5
38-39	2.068456668509621E-4	7.057087457268119E-4	0.0	0.016231301151716674	3.650217650311096E-5
40-41	2.068456668509621E-4	8.030478830684411E-4	0.0	0.0221507374413045	3.650217650311096E-5
42-43	2.068456668509621E-4	8.334663634877002E-4	0.0	0.027857244367957515	3.650217650311096E-5
44-45	2.068456668509621E-4	9.79475069500144E-4	0.0	0.034032195893067115	3.650217650311096E-5
46-47	2.068456668509621E-4	0.001082897902925625	0.0	0.04083985181089731	3.650217650311096E-5
48-49	2.068456668509621E-4	0.001082897902925625	0.0	0.04760492185614054	3.650217650311096E-5
50-51	2.494315394379249E-4	0.0011741533441834025	0.0	0.05385896143034022	3.650217650311096E-5
52-53	2.555152355217767E-4	0.0011802370402672544	0.0	0.05990007164160509	3.650217650311096E-5
54-55	2.615989316056285E-4	0.0011802370402672544	0.0	0.06604460468629543	4.258587258696278E-5
56-57	2.6768262768948035E-4	0.001192404432434958	0.0	0.07236556491741747	4.866956867081461E-5
58-59	2.9201741202488767E-4	0.0012045718246026615	0.0	0.07881428276630041	4.866956867081461E-5
60-61	3.1026850027644315E-4	0.0012471576971896244	0.0	0.08516566147784171	6.0836960838518265E-5
62-63	3.2243589244414683E-4	0.0012775761776088836	0.0	0.0909269216692494	6.0836960838518265E-5
64-65	3.285195885279986E-4	0.0013262457462796982	0.0	0.09656650793898003	6.0836960838518265E-5
66-67	3.4068698069570226E-4	0.0013262457462796982	0.0	0.1027232083758381	6.0836960838518265E-5
68-69	3.4068698069570226E-4	0.001362747922782809	0.0	0.10820461854738858	6.0836960838518265E-5
70-71	3.467706767795541E-4	0.0013931664032020682	0.0	0.11360085697376515	6.0836960838518265E-5
72-73	3.5285437286340594E-4	0.0014114174914536238	0.0	0.11778035618337136	6.0836960838518265E-5
74-75	3.5285437286340594E-4	0.0014418359718728828	0.0	0.12126023034333461	6.0836960838518265E-5
76-77	3.589380689472578E-4	0.0014905055405436975	0.0	0.12452109144427917	6.0836960838518265E-5
78-79	3.650217650311096E-4	0.0015148403248791049	0.0	0.12657738072062108	6.0836960838518265E-5
80-81	3.711054611149614E-4	0.0015270077170468084	0.0	0.12717966663292243	6.0836960838518265E-5
82-83	3.8327285328266505E-4	0.001539175109214512	0.0	0.12771503188830138	6.0836960838518265E-5
84-85	3.893565493665169E-4	0.001545258805298364	0.0	0.12789754277081694	6.0836960838518265E-5
86-87	3.893565493665169E-4	0.0015695935896337713	0.0	0.127976630819907	6.0836960838518265E-5
88	3.893565493665169E-4	0.001581760981801475	0.0	0.12798879821207473	6.0836960838518265E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8540	0.0	44.08612	1
GTATCAA	15220	0.0	35.61235	1
TCAACGC	17780	0.0	29.476469	4
ATCAACG	17810	0.0	29.42682	3
CAACGCA	18320	0.0	28.63038	5
TATCAAC	18940	0.0	28.17747	2
AACGCAG	18825	0.0	27.884491	6
CTTATAC	5920	0.0	26.199512	1
ACGCAGA	22130	0.0	23.682407	7
CGCAGAG	22695	0.0	23.111197	8
GTACATG	21020	0.0	20.906431	1
TATACAC	8915	0.0	20.765081	3
TACATGG	20960	0.0	20.667894	2
GTGGTAT	5020	0.0	20.514704	1
ACATGGG	21065	0.0	19.911709	3
TGGTATC	4805	0.0	19.176567	2
GCAGAGT	27255	0.0	19.030325	9
TATGCCG	4575	0.0	18.161297	42-43
GAGTACT	15760	0.0	17.817879	12-13
CATGGGG	15770	0.0	17.766813	4
>>END_MODULE