FastQCFastQC Report
Fri 27 May 2016
SRR2140210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7441695
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74250.044.937961
GTATCAA127950.034.180781
TCAACGC147950.028.7259464
ATCAACG149750.028.4356063
CAACGCA152250.027.9146395
AACGCAG157650.027.0628666
TATCAAC161750.026.7075462
ACGCAGA185950.022.944137
CGCAGAG191450.022.2420068
GTACATG189550.021.5533331
TACATGG188450.021.1333452
CTTATAC51750.019.9550291
ACATGGG194850.019.826873
GCAGAGT226900.018.5312799
CATGGGG147800.018.3164144
GAGTACT131850.018.17639412-13
TATGCCG33150.017.22131342-43
TATACAC77650.016.9549883
AGAGTAC200800.016.83188810-11
CAGAGTA223100.016.68007710-11
Produced by FastQC (version 0.11.5)