##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7441695
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36047768687107	33.0	31.0	34.0	31.0	34.0
2	32.65307487071158	34.0	31.0	34.0	31.0	34.0
3	32.3233127667823	33.0	31.0	34.0	30.0	34.0
4	36.18846633730622	37.0	35.0	37.0	35.0	37.0
5	36.073521959714824	37.0	35.0	37.0	35.0	37.0
6	36.16307077890185	37.0	36.0	37.0	35.0	37.0
7	36.140952968376155	37.0	36.0	37.0	35.0	37.0
8	36.14432975283185	37.0	36.0	37.0	35.0	37.0
9	37.963905803718106	39.0	38.0	39.0	35.0	39.0
10-11	37.8935828463811	39.0	38.0	39.0	35.0	39.0
12-13	37.86008301872087	39.0	38.0	39.0	35.0	39.0
14-15	39.25199836865123	41.0	39.0	41.0	36.0	41.0
16-17	39.24173121849256	41.0	39.0	41.0	36.0	41.0
18-19	39.21177466961492	41.0	39.0	41.0	36.0	41.0
20-21	39.201860860939625	40.0	39.0	41.0	36.0	41.0
22-23	39.13969452356736	40.0	39.0	41.0	36.0	41.0
24-25	39.087370746203874	40.0	39.0	41.0	36.0	41.0
26-27	38.93612861637483	40.0	38.0	41.0	35.0	41.0
28-29	38.81933455301694	40.0	38.0	41.0	35.0	41.0
30-31	38.68984946549304	40.0	38.0	41.0	35.0	41.0
32-33	38.53814731954202	40.0	38.0	41.0	34.0	41.0
34-35	38.62512106504256	40.0	38.0	41.0	35.0	41.0
36-37	38.619085498087045	40.0	38.0	41.0	35.0	41.0
38-39	38.57271279878772	40.0	38.0	41.0	34.0	41.0
40-41	38.51084285054384	40.0	38.0	41.0	34.0	41.0
42-43	38.48317383613972	40.0	38.0	41.0	34.0	41.0
44-45	38.533463862727956	40.0	38.0	41.0	34.0	41.0
46-47	38.49076730012706	40.0	38.0	41.0	34.0	41.0
48-49	38.41228635984886	40.0	38.0	41.0	34.0	41.0
50-51	38.319463027801845	40.0	38.0	41.0	34.0	41.0
52-53	38.12789346582305	40.0	37.0	41.0	33.0	41.0
54-55	38.02201501967264	40.0	37.0	41.0	33.0	41.0
56-57	37.88390880326577	40.0	37.0	41.0	33.0	41.0
58-59	37.70064757692052	39.0	36.0	41.0	33.0	41.0
60-61	37.506002898217346	39.0	36.0	41.0	33.0	41.0
62-63	37.22341412829054	39.0	35.0	41.0	32.0	41.0
64-65	36.914003574949106	38.5	35.0	40.0	32.0	41.0
66-67	36.62830926518341	38.0	35.0	40.0	31.0	41.0
68-69	36.33984007220252	37.0	35.0	40.0	31.0	41.0
70-71	36.034946830991316	37.0	35.0	39.0	31.0	41.0
72-73	35.69390451910739	36.0	34.5	39.0	31.0	41.0
74-75	35.34749927059095	36.0	34.0	39.0	31.0	40.0
76-77	34.16733762255328	35.0	32.5	37.0	29.5	39.0
78-79	34.765209648950886	35.0	34.0	37.0	30.5	39.0
80-81	34.62408218785373	35.0	34.0	37.0	31.0	39.0
82-83	34.39267500328622	35.0	34.0	36.0	30.0	38.5
84-85	34.18539955852122	35.0	34.0	36.0	30.0	37.0
86-87	33.963596381644756	35.0	34.0	35.5	30.0	37.0
88-89	33.79878296936805	35.0	34.0	35.0	30.0	36.5
90-91	33.63300231964118	35.0	34.0	35.0	30.0	36.0
92-93	33.52007423560529	35.0	34.0	35.0	30.0	36.0
94-95	33.4927616697652	35.0	33.5	35.0	30.0	36.0
96-97	33.503874066372916	35.0	33.0	35.0	30.0	35.5
98-99	33.56125885693595	35.0	33.0	35.0	30.0	35.0
100	32.46081021159659	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	3.0
20	28.0
21	167.0
22	698.0
23	2503.0
24	6749.0
25	14792.0
26	27226.0
27	43450.0
28	64028.0
29	87592.0
30	113057.0
31	143370.0
32	182226.0
33	235008.0
34	324646.0
35	472648.0
36	787224.0
37	1514877.0
38	2103608.0
39	1258906.0
40	58885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10933127466256	19.983767139072484	13.55407605390976	24.352825532355197
2	15.17011917311849	21.06886939064286	40.40090328883406	23.36010814740459
3	18.38571723243159	25.52994982997825	30.408784557819153	25.67554837977101
4	12.708878823977601	17.53963310777988	38.33997496538087	31.411513102861647
5	13.322650283302393	37.63313331169847	35.69731089489693	13.3469055101022
6	30.773472978938265	37.473801331551485	18.352727436424093	13.39999825308616
7	27.007341741364034	32.26647961250764	22.851648717126945	17.87452992900139
8	24.81574426256384	36.41759034736038	21.0775636464542	17.68910174362158
9	25.45417408265187	16.619654527631138	21.164129946201772	36.76204144351522
10-11	24.430052561949932	26.60008237370653	28.778960975960448	20.190904088383093
12-13	24.989159055833383	23.97272395603421	28.991815708652396	22.04630127948001
14-15	22.078128705892944	25.541163673061046	26.324002797749706	26.056704823296307
16-17	22.001741538722026	28.703736178384094	27.0666696229824	22.227852659911484
18-19	22.01360450061176	27.966749331969837	28.546459087393867	21.473187080024537
20-21	22.65079311966224	27.170148722619032	28.454443678015416	21.724614479703316
22-23	22.372263031228716	27.160829894255713	28.33862228361026	22.12828479090531
24-25	22.193977289568586	27.227837266440762	28.47576502106944	22.10242042292121
26-27	22.19713706899543	27.416670679274958	28.364968826741134	22.021223424988477
28-29	22.135611547275378	27.431182898534946	28.375995217182933	22.05721033700675
30-31	22.38940231225956	27.34504860195622	28.25881932479863	22.006729760985593
32-33	22.190195332597575	27.445098508795084	28.21248744629763	22.15221871230971
34-35	22.236770029783067	27.511601970898013	28.31404756077658	21.937580438542337
36-37	22.308678032853145	27.418498349876725	28.18798613446582	22.084837482804307
38-39	22.09344442418783	27.52909623383572	28.292816542750685	22.084642799225758
40-41	22.339252816652643	27.37163646228665	28.168051390467326	22.121059330593386
42-43	22.26289596097984	27.478436265426488	28.02814703667901	22.23052073691467
44-45	22.26020349460259	27.62275461109792	27.8807246456992	22.236317248600283
46-47	22.408452008322524	27.563381926515657	27.613327732759913	22.414838332401903
48-49	22.35976590991121	27.58513216482613	27.644617321993238	22.410484603269417
50-51	22.29281787088947	27.697080194623236	27.659361884892768	22.35074004959453
52-53	22.458264480160906	27.657184012702835	27.583263326197123	22.30128818093914
54-55	22.412540256763087	27.57233465791616	27.538827008068544	22.47629807725221
56-57	22.453588035592393	27.751258974207378	27.42610909686905	22.369043893331177
58-59	22.327424535903386	27.817116392317548	27.571763208429896	22.283695863349166
60-61	22.4389309086588	27.882051750467273	27.42293219631161	22.25608514456231
62-63	22.380567347479126	28.018264064866443	27.369163332538175	22.232005255116253
64-65	22.36811031226441	27.880433981778435	27.417491410727518	22.333964295229634
66-67	22.39211419953566	27.885427908361876	27.298208723438556	22.424249168663913
68-69	22.384227372174053	27.84965078377608	27.39868057171087	22.367441272338993
70-71	22.3921263409597	27.567436186474993	27.485783597641188	22.554653874924117
72-73	22.421492227227972	27.647471361587794	27.42744088616977	22.503595525014465
74-75	22.31432432070762	27.786615235317562	27.378664606431435	22.520395837543383
76-77	22.41917750944065	27.707656396555947	27.351210049891	22.521956044112397
78-79	22.338124598110994	27.725748035370835	27.335855490152056	22.60027187636612
80-81	22.314914483387582	27.755832780409673	27.373405394834975	22.55584734136777
82-83	22.418222942114177	27.728485131800323	27.434997723218963	22.418294202866534
84-85	22.497254603608614	27.69488930848673	27.37266896199339	22.435187125911263
86-87	22.367992806994234	27.765069935099618	27.402913946480624	22.464023311425528
88-89	22.395254876800237	27.720419422075654	27.404019739866882	22.480305961257226
90-91	22.401988180337998	27.808865867840172	27.41659526378641	22.37255068803542
92-93	22.44280340129784	27.706544257861808	27.460470825993067	22.39018151484729
94-95	22.28501673551638	27.790248194695693	27.49878201980155	22.425953049986376
96-97	22.329744851913198	27.91672360483957	27.579429376893668	22.174102166353567
98-99	23.425114966560905	29.858096132509456	27.77636347291811	18.940425428011537
100	29.94722729993493	41.359901622527026	28.692871077538047	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1900.0
1	1590.5
2	1874.5
3	2676.0
4	3456.0
5	5229.0
6	6966.5
7	8394.5
8	9491.5
9	9498.5
10	8607.0
11	7708.0
12	8147.0
13	10600.5
14	16041.5
15	23346.0
16	29500.0
17	33317.5
18	34759.5
19	34156.5
20	32633.0
21	31459.0
22	31870.0
23	35216.5
24	41946.5
25	52100.0
26	65833.0
27	81323.0
28	97114.0
29	114120.0
30	130684.5
31	145488.5
32	160704.5
33	176817.0
34	191847.0
35	206934.0
36	223726.0
37	238094.0
38	250593.5
39	262426.5
40	274909.5
41	288099.0
42	296095.5
43	303202.0
44	313619.5
45	323609.5
46	329091.5
47	332281.0
48	331715.0
49	327990.0
50	323873.5
51	315460.5
52	302481.0
53	286257.0
54	269414.5
55	252064.0
56	232325.0
57	211061.0
58	189077.0
59	164099.5
60	139123.0
61	115502.0
62	93058.0
63	73154.0
64	56021.0
65	43070.0
66	33200.0
67	25603.5
68	19579.5
69	14781.0
70	11126.0
71	8117.0
72	5786.5
73	4068.0
74	2809.5
75	1943.0
76	1319.5
77	862.5
78	575.0
79	386.5
80	255.0
81	154.5
82	111.5
83	90.0
84	62.5
85	39.5
86	28.5
87	18.5
88	10.0
89	7.0
90	5.5
91	4.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.37511951242291E-4
20-21	0.0
22-23	6.249265879250206E-4
24-25	0.0065191874135325555
26-27	0.02865391542778709
28-29	0.027808575268386728
30-31	0.028482105990511587
32-33	0.04955075939616252
34-35	0.023673272698507865
36-37	0.01906451667588107
38-39	0.014931561721351816
40-41	0.007638103455430657
42-43	0.010328907863891854
44-45	0.004586460867071494
46-47	0.00377261281804242
48-49	0.007417787343986918
50-51	0.0019525604440874
52-53	0.0020175346842874623
54-55	0.001105875630066998
56-57	0.006474881340761516
58-59	0.004341119639409977
60-61	0.007194639526368471
62-63	0.005866850373895556
64-65	0.0011549137598018328
66-67	0.004483373096390828
68-69	0.011107815066640356
70-71	0.014781317969144515
72-73	0.004805244044866915
74-75	0.0014736720859404907
76-77	0.0024170499733653095
78-79	0.0025949346874900195
80-81	0.003613730381774349
82-83	0.0031799099524322883
84-85	0.004186379416077664
86-87	4.57244360165164E-5
88-89	2.8758464613405063E-4
90-91	0.003731373872072108
92-93	7.665183262075874E-4
94-95	0.0021763114838491827
96-97	5.606188671674661E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	179.0
20-21	458.0
22-23	1023.0
24-25	2215.0
26-27	4511.0
28-29	10841.0
30-31	23273.0
32-33	29673.0
34-35	40602.0
36-37	61622.0
38-39	69529.0
40-41	68034.0
42-43	77141.0
44-45	86180.0
46-47	84679.0
48-49	77676.0
50-51	70997.0
52-53	68690.0
54-55	73855.0
56-57	75802.0
58-59	74735.0
60-61	72997.0
62-63	71146.0
64-65	75330.0
66-67	78826.0
68-69	77922.0
70-71	73679.0
72-73	69226.0
74-75	69885.0
76-77	71110.0
78-79	74604.0
80-81	73989.0
82-83	71614.0
84-85	72975.0
86-87	76965.0
88-89	81539.0
90-91	87230.0
92-93	91842.0
94-95	111392.0
96-97	261849.0
98-99	1553171.0
100-101	3222689.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.8346318974101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.2797882733559	80.17526602663747
2	6.314345821751581	11.092364818529992
3	1.4243294360321235	3.753163551435819
4	0.47447476309509934	1.6670126464427566
5	0.21305389717267403	0.9356755316235245
6	0.10478367878074905	0.5522181512738134
7	0.060339022676647515	0.37098990920969704
8	0.03543480651196963	0.24899225490678428
9	0.021607050179503443	0.17080625690149984
>10	0.07084431734978217	0.9252165359878242
>50	7.679530952525822E-4	0.043943104966257605
>100	2.0900999994001758E-4	0.04408908119736835
>500	0.0	0.0
>1k	2.196999872911868E-5	0.020262130887166423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.375119512422909E-5	0.0	0.0	0.0
3	0.0	5.375119512422909E-5	0.0	0.0	0.0
4	0.0	8.062679268634363E-5	0.0	0.0	0.0
5	1.3437798781057273E-5	8.062679268634363E-5	0.0	0.0	0.0
6	1.3437798781057273E-5	8.062679268634363E-5	0.0	0.0	0.0
7	1.3437798781057273E-5	8.062679268634363E-5	0.0	0.0	0.0
8	1.3437798781057273E-5	1.2094018902951545E-4	0.0	0.0	0.0
9	1.3437798781057273E-5	2.687559756211455E-4	0.0	0.0	0.0
10-11	2.6875597562114546E-5	2.687559756211455E-4	0.0	3.3594496952643184E-5	0.0
12-13	2.6875597562114546E-5	2.687559756211455E-4	0.0	4.0313396343171816E-5	0.0
14-15	2.6875597562114546E-5	3.359449695264318E-4	0.0	6.047009451475773E-5	1.3437798781057273E-5
16-17	3.3594496952643184E-5	3.4266386891696046E-4	0.0	6.718899390528637E-5	1.3437798781057273E-5
18-19	4.0313396343171816E-5	3.8969616465066087E-4	0.0	8.734569207687228E-5	1.3437798781057273E-5
20-21	4.0313396343171816E-5	4.232906616033041E-4	0.0	1.276590884200441E-4	1.3437798781057273E-5
22-23	4.0313396343171816E-5	4.501662591654186E-4	0.0	1.8812918293480182E-4	2.6875597562114546E-5
24-25	4.7032295733700454E-5	4.636040579464759E-4	0.0	2.21723679887445E-4	4.0313396343171816E-5
26-27	5.375119512422909E-5	4.7032295733700455E-4	0.0	2.687559756211455E-4	4.0313396343171816E-5
28-29	5.375119512422909E-5	4.7032295733700455E-4	0.0	6.853277378339209E-4	4.0313396343171816E-5
30-31	5.375119512422909E-5	5.576686494138767E-4	0.0	0.0022306745976555073	4.0313396343171816E-5
32-33	5.375119512422909E-5	5.643875488044054E-4	0.0	0.004468068094701543	4.0313396343171816E-5
34-35	6.047009451475773E-5	6.382954421002205E-4	0.0	0.007518448418001544	5.375119512422909E-5
36-37	6.718899390528637E-5	7.122033353960355E-4	0.0	0.012188083494418946	5.375119512422909E-5
38-39	6.718899390528637E-5	7.122033353960355E-4	0.0	0.01896745297946234	5.375119512422909E-5
40-41	6.718899390528637E-5	7.659545305202646E-4	0.0	0.026291053315138556	5.375119512422909E-5
42-43	6.718899390528637E-5	8.062679268634363E-4	0.0	0.03279494792517027	5.375119512422909E-5
44-45	6.718899390528637E-5	9.540837134550664E-4	0.0	0.039930419077911686	5.375119512422909E-5
46-47	6.718899390528637E-5	0.0010615861037035245	0.0	0.04881952297158107	6.047009451475773E-5
48-49	6.718899390528637E-5	0.0010615861037035245	0.0	0.056848607743262794	9.406459146740091E-5
50-51	6.718899390528637E-5	0.0012429963872477977	0.0	0.06396392219783262	1.0750239024845818E-4
52-53	7.3907893295815E-5	0.0012631530854193835	0.0	0.07119345794204143	1.276590884200441E-4
54-55	8.062679268634363E-5	0.0012631530854193835	0.0	0.07834236689356389	1.3437798781057274E-4
56-57	8.062679268634363E-5	0.0012631530854193835	0.0	0.08539049235422844	1.3437798781057274E-4
58-59	8.062679268634363E-5	0.0012631530854193835	0.0	0.0927476871868573	1.3437798781057274E-4
60-61	8.062679268634363E-5	0.0013034664817625553	0.0	0.09937252198591853	1.3437798781057274E-4
62-63	8.062679268634363E-5	0.0013437798781057273	0.0	0.10623923716303879	1.3437798781057274E-4
64-65	8.062679268634363E-5	0.0013975310732299564	0.0	0.11301188774869167	1.3437798781057274E-4
66-67	8.062679268634363E-5	0.0014109688720110136	0.0	0.11967031704470554	1.3437798781057274E-4
68-69	8.062679268634363E-5	0.0014378444695731282	0.0	0.12596592577363086	1.3437798781057274E-4
70-71	8.062679268634363E-5	0.0014445633689636568	0.0	0.13179121154521922	1.3437798781057274E-4
72-73	8.062679268634363E-5	0.0014512822683541854	0.0	0.13641381432590288	1.4781578659163E-4
74-75	8.062679268634363E-5	0.0014781578659163	0.0	0.1403107759724095	1.4781578659163E-4
76-77	8.062679268634363E-5	0.001552065759212115	0.0	0.14406664073171502	1.4781578659163E-4
78-79	8.062679268634363E-5	0.0015856602561647583	0.0	0.14659294690255378	1.4781578659163E-4
80-81	8.062679268634363E-5	0.0015856602561647583	0.0	0.14750671721966568	1.4781578659163E-4
82-83	8.062679268634363E-5	0.0015856602561647583	0.0	0.1479770401770027	1.6125358537268726E-4
84-85	8.062679268634363E-5	0.001605816954336344	0.0	0.1481920449574996	1.6125358537268726E-4
86-87	8.062679268634363E-5	0.00162597365250793	0.0	0.14827939064957646	1.6125358537268726E-4
88	8.062679268634363E-5	0.0016662870488511018	0.0	0.148286109548967	1.6125358537268726E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7425	0.0	44.93796	1
GTATCAA	12795	0.0	34.18078	1
TCAACGC	14795	0.0	28.725946	4
ATCAACG	14975	0.0	28.435606	3
CAACGCA	15225	0.0	27.914639	5
AACGCAG	15765	0.0	27.062866	6
TATCAAC	16175	0.0	26.707546	2
ACGCAGA	18595	0.0	22.94413	7
CGCAGAG	19145	0.0	22.242006	8
GTACATG	18955	0.0	21.553333	1
TACATGG	18845	0.0	21.133345	2
CTTATAC	5175	0.0	19.955029	1
ACATGGG	19485	0.0	19.82687	3
GCAGAGT	22690	0.0	18.531279	9
CATGGGG	14780	0.0	18.316414	4
GAGTACT	13185	0.0	18.176394	12-13
TATGCCG	3315	0.0	17.221313	42-43
TATACAC	7765	0.0	16.954988	3
AGAGTAC	20080	0.0	16.831888	10-11
CAGAGTA	22310	0.0	16.680077	10-11
>>END_MODULE