FastQCFastQC Report
Fri 27 May 2016
SRR2140209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998499
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70650.044.551551
GTATCAA124100.034.4285621
TCAACGC145000.028.9811784
ATCAACG146850.028.6160773
CAACGCA150150.027.9871545
AACGCAG154900.027.3158426
TATCAAC155600.027.2727012
CTTATAC55550.024.943141
ACGCAGA180500.023.327117
CGCAGAG185350.022.7167178
TATACAC76800.021.6498663
GTACATG179650.020.6056421
TACATGG178600.020.1941412
ACATGGG182450.019.065253
GCAGAGT221150.018.833589
TATGCCG42500.018.67378442-43
GTGGTAT38100.018.5635681
TTATACA84050.018.207672
CTCGTAT42450.017.86488538-39
GAGTACT129350.017.63488212-13
Produced by FastQC (version 0.11.5)