##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140209_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6998499 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36159625085322 33.0 31.0 34.0 31.0 34.0 2 32.653463835602466 34.0 31.0 34.0 31.0 34.0 3 32.32401290619603 33.0 31.0 34.0 30.0 34.0 4 36.18777897946403 37.0 35.0 37.0 35.0 37.0 5 36.0733955952555 37.0 35.0 37.0 35.0 37.0 6 36.16233866719135 37.0 36.0 37.0 35.0 37.0 7 36.14266444847674 37.0 36.0 37.0 35.0 37.0 8 36.146059747954524 37.0 36.0 37.0 35.0 37.0 9 37.966076440105226 39.0 38.0 39.0 35.0 39.0 10-11 37.89666662808696 39.0 38.0 39.0 35.0 39.0 12-13 37.86196968807168 39.0 38.0 39.0 35.0 39.0 14-15 39.255204080189195 41.0 39.0 41.0 36.0 41.0 16-17 39.24436354138223 41.0 39.0 41.0 36.0 41.0 18-19 39.21419042854761 41.0 39.0 41.0 36.0 41.0 20-21 39.20396778131362 40.0 39.0 41.0 36.0 41.0 22-23 39.14147817601534 40.0 39.0 41.0 36.0 41.0 24-25 39.08762586016894 40.0 39.0 41.0 35.5 41.0 26-27 38.93428899916921 40.0 38.0 41.0 35.0 41.0 28-29 38.81523900608457 40.0 38.0 41.0 35.0 41.0 30-31 38.686293508703415 40.0 38.0 41.0 35.0 41.0 32-33 38.53295167714464 40.0 38.0 41.0 34.0 41.0 34-35 38.6219860459135 40.0 38.0 41.0 35.0 41.0 36-37 38.6118733041445 40.0 38.0 41.0 35.0 41.0 38-39 38.56550395573733 40.0 38.0 41.0 34.0 41.0 40-41 38.50678839204431 40.0 38.0 41.0 34.0 41.0 42-43 38.47880131936652 40.0 38.0 41.0 34.0 41.0 44-45 38.52937845794837 40.0 38.0 41.0 34.0 41.0 46-47 38.48487660121937 40.0 38.0 41.0 34.0 41.0 48-49 38.40827656743073 40.0 38.0 41.0 34.0 41.0 50-51 38.315258144703016 40.0 38.0 41.0 34.0 41.0 52-53 38.1282743369908 40.0 37.0 41.0 33.0 41.0 54-55 38.02433487177197 40.0 37.0 41.0 33.0 41.0 56-57 37.88509367217863 40.0 37.0 41.0 33.0 41.0 58-59 37.70483324753128 39.0 36.0 41.0 33.0 41.0 60-61 37.508108301906006 39.0 36.0 41.0 33.0 41.0 62-63 37.23141961226183 39.0 35.0 41.0 32.0 41.0 64-65 36.927179285032906 38.5 35.0 40.0 32.0 41.0 66-67 36.644929605861535 38.0 35.0 40.0 31.0 41.0 68-69 36.35916601483462 37.0 35.0 40.0 31.0 41.0 70-71 36.05547387514952 37.0 35.0 39.0 31.0 41.0 72-73 35.716193589061334 36.0 34.5 39.0 31.0 41.0 74-75 35.37008489380698 36.0 34.0 39.0 30.5 40.0 76-77 34.1826189180002 35.0 32.5 37.0 29.5 39.0 78-79 34.78456867579826 35.0 34.0 37.0 30.5 39.0 80-81 34.64045524719261 35.0 34.0 37.0 31.0 39.0 82-83 34.40347861381882 35.0 34.0 36.0 30.0 38.5 84-85 34.19237605956582 35.0 34.0 36.0 30.0 37.0 86-87 33.96718463148099 35.0 34.0 36.0 30.0 37.0 88-89 33.79837156026678 35.0 34.0 35.0 30.0 36.5 90-91 33.63014468036161 35.0 34.0 35.0 30.0 36.0 92-93 33.51483611685791 35.0 34.0 35.0 30.0 36.0 94-95 33.48590470054912 35.0 33.5 35.0 30.0 36.0 96-97 33.49989921796046 35.0 33.0 35.0 30.0 35.5 98-99 33.55696336208185 35.0 33.0 35.0 30.0 35.0 100 32.41862142706067 34.0 31.0 35.0 27.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 6.0 20 24.0 21 168.0 22 710.0 23 2449.0 24 6418.0 25 14129.0 26 25585.0 27 41757.0 28 61089.0 29 83226.0 30 107739.0 31 136401.0 32 172069.0 33 224021.0 34 307401.0 35 446196.0 36 739644.0 37 1422709.0 38 1986260.0 39 1167111.0 40 53387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09381183022245 19.944319489079014 13.57206738187717 24.38980129882136 2 15.123357165586507 20.956193606657656 40.57821541447673 23.342233813279105 3 18.444540750809566 25.41349223597803 30.311685405684845 25.830281607527557 4 12.656556784533368 17.56199436479165 38.26937747651318 31.512071374161803 5 13.333073277569948 37.58363043275422 35.71680155987734 13.36649472979849 6 30.78137183416044 37.40396333556667 18.377083428889538 13.437581401383353 7 27.08425049428456 32.20447698856569 22.658515776025688 18.05275674112406 8 24.75942341350624 36.579872341197735 20.983813814933743 17.67689043036228 9 25.367067995580193 16.70718249727549 21.127544634928146 36.798204872216175 10-11 24.388501020004433 26.62843846944895 28.769497573694014 20.213562936852604 12-13 24.96017360293972 23.959444732363323 28.936533390945684 22.14384827375127 14-15 22.031767097487617 25.55969501460242 26.310720341604675 26.097817546305286 16-17 21.991479887330126 28.794124282935528 27.042084309792713 22.172311519941633 18-19 21.9897053117445 28.0126130542797 28.528004754186405 21.469676879789397 20-21 22.540236375196415 27.175381347749152 28.527971717454324 21.756410559600106 22-23 22.31421168483313 27.226252254165388 28.322170698215004 22.137365362786472 24-25 22.16625824709852 27.328883135970443 28.4831928325707 22.021665784360327 26-27 22.166746997601514 27.500897465785457 28.385369662939024 21.946985873674002 28-29 22.071486696836672 27.493915081002363 28.438633150894898 21.995965071266067 30-31 22.371106599101232 27.417041631894556 28.27402716956458 21.937824599439633 32-33 22.161423308914973 27.39634282846664 28.306470548627527 22.13576331399086 34-35 22.127239253949856 27.597761352698313 28.431418713189455 21.843580680162376 36-37 22.270996114290895 27.495920190712965 28.225196318003455 22.007887376992688 38-39 22.013214369615746 27.52291682207366 28.382214297951442 22.081654510359154 40-41 22.28653344407407 27.40955185419133 28.255863741871067 22.048050959863534 42-43 22.245773448432157 27.478158497447026 28.11465360528929 22.161414448831522 44-45 22.17487198234949 27.660231597316574 28.01010652559214 22.154789894741796 46-47 22.36001284886673 27.52594124513702 27.71749867251787 22.39654723347838 48-49 22.25925311890282 27.629694087642402 27.686172211018146 22.424880582436632 50-51 22.15859294188969 27.734035574159606 27.781727617946693 22.325643866004008 52-53 22.337030839189826 27.65777889071041 27.802223674079094 22.20296659602067 54-55 22.29828762866989 27.628905558628375 27.66305378102192 22.40975303167982 56-57 22.36774034376825 27.795522810355948 27.501274833084405 22.335462012791396 58-59 22.22301717222231 27.90113339792777 27.726770747055486 22.14907868279444 60-61 22.36302146506376 28.016688692986573 27.49864281924822 22.121647022701445 62-63 22.26625944282397 28.183170165540872 27.448492722761674 22.102077668873484 64-65 22.25542547488422 28.048183990230907 27.465455123851555 22.23093541103332 66-67 22.315809167031794 28.013266805630018 27.35735547182465 22.313568555513534 68-69 22.26367882283965 27.925438871275876 27.495175621861957 22.31570668402252 70-71 22.293195241813095 27.683314098607987 27.5886348553498 22.434855804229123 72-73 22.37743018470491 27.72552939718389 27.478442312666512 22.418598105444683 74-75 22.234515113671005 27.82800818506324 27.496082236821522 22.44139446444423 76-77 22.342154593684953 27.78168863782212 27.452220785694596 22.423935982798326 78-79 22.279313689271653 27.762388965309174 27.439275772441906 22.519021572977266 80-81 22.257203643691557 27.83864223757746 27.458512438921943 22.445641679809032 82-83 22.344117417635793 27.85087807813581 27.52136586865901 22.28363863556939 84-85 22.408881540276436 27.801095937121843 27.449452920026467 22.34056960257525 86-87 22.26070172165111 27.83488886469077 27.531282725827406 22.373126687830712 88-89 22.3052783077545 27.809333361974858 27.525577208115187 22.359811122155456 90-91 22.316516335947014 27.869851845476333 27.518206470758788 22.29542534781786 92-93 22.365743644456916 27.82259861104139 27.543260554837495 22.2683971896642 94-95 22.19830831457076 27.89661226674659 27.624501468074197 22.280577950608453 96-97 22.267325830246573 28.03764187218342 27.65968441905105 22.035347878518955 98-99 23.299108233485207 29.98390651979181 27.87480026923684 18.842184977486152 100 22.256567836355604 31.064058893715597 21.616122830389777 25.06325043953902 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1820.0 1 1542.5 2 1793.0 3 2481.5 4 3146.0 5 4850.0 6 6486.5 7 7739.5 8 8756.5 9 8732.5 10 7849.0 11 7143.0 12 7703.0 13 9830.5 14 14561.5 15 21156.0 16 26924.0 17 30623.0 18 32025.0 19 31410.0 20 29947.0 21 28800.0 22 29096.5 23 32053.5 24 37955.5 25 47112.5 26 59042.0 27 72693.5 28 87816.5 29 104083.0 30 119574.0 31 134879.0 32 150700.5 33 164653.5 34 178526.5 35 192553.0 36 207563.0 37 222323.0 38 235413.0 39 247528.0 40 259554.0 41 270474.5 42 277384.0 43 284233.0 44 292821.5 45 304322.5 46 312429.5 47 313895.5 48 312156.5 49 307542.0 50 302913.5 51 296027.0 52 284359.0 53 268891.0 54 252375.5 55 234914.0 56 216664.5 57 197027.0 58 175508.5 59 153787.0 60 130850.0 61 107942.5 62 87272.0 63 68962.0 64 53298.5 65 40899.5 66 31269.5 67 24224.5 68 18751.0 69 14120.0 70 10487.5 71 7684.0 72 5515.0 73 3860.5 74 2701.5 75 1857.5 76 1248.5 77 834.0 78 554.0 79 360.5 80 227.5 81 138.0 82 89.5 83 69.5 84 55.0 85 36.5 86 23.5 87 15.5 88 10.0 89 5.5 90 4.0 91 3.0 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.072516263844576E-4 20-21 0.0 22-23 6.073473162786873E-4 24-25 0.006496268611916773 26-27 0.02890362881020496 28-29 0.02748022832986176 30-31 0.029257366054373977 32-33 0.05094872039333621 34-35 0.024115669726805563 36-37 0.019845647065797112 38-39 0.014990100379456148 40-41 0.007881264098572286 42-43 0.011231180682906532 44-45 0.004744941300399065 46-47 0.003610559964263853 48-49 0.008493390636438329 50-51 0.0022241744664303004 52-53 0.002285715907108821 54-55 0.0011703730337135905 56-57 0.007147408786628357 58-59 0.004761333866928287 60-61 0.007959298435993741 62-63 0.006233308838399181 64-65 0.001455917306590012 66-67 0.00454065289988414 68-69 0.01181252750899508 70-71 0.015046439498633761 72-73 0.005321402078069388 74-75 0.0017789412510462452 76-77 0.0026587514250093736 78-79 0.0031394998785846473 80-81 0.0036429284918164813 82-83 0.0034448158337627623 84-85 0.0039818533921494575 86-87 2.8883685974255393E-5 88-89 2.245750965941429E-4 90-91 0.003926517797437689 92-93 6.854627266537947E-4 94-95 0.002360769754639041 96-97 5.262391247422481E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 175.0 20-21 497.0 22-23 1038.0 24-25 2151.0 26-27 4043.0 28-29 9686.0 30-31 20817.0 32-33 26971.0 34-35 36931.0 36-37 55986.0 38-39 62200.0 40-41 61104.0 42-43 69671.0 44-45 77481.0 46-47 76089.0 48-49 70585.0 50-51 63996.0 52-53 62645.0 54-55 67216.0 56-57 68801.0 58-59 68534.0 60-61 67595.0 62-63 66037.0 64-65 70153.0 66-67 74256.0 68-69 74167.0 70-71 67630.0 72-73 62997.0 74-75 64185.0 76-77 65039.0 78-79 68735.0 80-81 67846.0 82-83 65509.0 84-85 66862.0 86-87 71456.0 88-89 76384.0 90-91 81865.0 92-93 85666.0 94-95 104307.0 96-97 246768.0 98-99 445244.0 100-101 4099181.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.61885178782639 #Duplication Level Percentage of deduplicated Percentage of total 1 91.79428288448676 81.3470394991014 2 6.046646527619336 10.716937448889459 3 1.2600537671613556 3.349935541102934 4 0.4498045315431032 1.5944464445724376 5 0.1883911849383564 0.8347505248092597 6 0.08715157061789836 0.4633963271798292 7 0.05552065667858902 0.34441237917538714 8 0.031906435839562215 0.22620093669951644 9 0.01932005822355401 0.15409092386207782 >10 0.0656434076682918 0.8647051363948678 >50 0.0010913250636318682 0.06262427400772681 >100 1.650841745177558E-4 0.030201849642864247 >500 2.2565985005998422E-5 0.01125871456230095 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.286633462403867E-5 0.0 2.857755641602578E-5 0.0 3 0.0 4.286633462403867E-5 0.0 2.857755641602578E-5 0.0 4 0.0 1.1431022566410312E-4 0.0 4.286633462403867E-5 0.0 5 0.0 1.28599003872116E-4 0.0 4.286633462403867E-5 0.0 6 0.0 1.28599003872116E-4 0.0 5.715511283205156E-5 0.0 7 0.0 1.28599003872116E-4 0.0 5.715511283205156E-5 0.0 8 0.0 1.428877820801289E-4 0.0 5.715511283205156E-5 0.0 9 0.0 1.7146533849615468E-4 0.0 7.144389104006445E-5 0.0 10-11 2.857755641602578E-5 1.8575411670416757E-4 0.0 1.1431022566410312E-4 7.144389104006445E-6 12-13 2.857755641602578E-5 2.0004289491218046E-4 0.0 1.1431022566410312E-4 1.428877820801289E-5 14-15 3.572194552003222E-5 3.5721945520032224E-4 0.0 1.428877820801289E-4 2.1433167312019334E-5 16-17 5.7155112832051554E-5 3.7150823340833513E-4 0.0 1.7146533849615468E-4 2.857755641602578E-5 18-19 7.144389104006445E-5 3.9294140072035447E-4 0.0 2.0004289491218046E-4 2.857755641602578E-5 20-21 7.144389104006445E-5 4.429521244483996E-4 0.0 2.1433167312019335E-4 2.857755641602578E-5 22-23 7.144389104006445E-5 5.286847936964769E-4 0.0 2.1433167312019335E-4 4.286633462403867E-5 24-25 7.858828014407089E-5 5.78695517424522E-4 0.0 2.2862045132820624E-4 5.715511283205156E-5 26-27 8.573266924807734E-5 6.001286847365414E-4 0.0 2.5005361864022555E-4 5.715511283205156E-5 28-29 8.573266924807734E-5 6.001286847365414E-4 0.0 6.858613539846187E-4 5.715511283205156E-5 30-31 8.573266924807734E-5 6.715725757766058E-4 0.0 0.0020004289491218044 5.715511283205156E-5 32-33 8.573266924807734E-5 6.930057430886251E-4 0.0 0.004143745680323737 5.715511283205156E-5 34-35 9.287705835208378E-5 8.287491360647476E-4 0.0 0.0070872339911743935 5.715511283205156E-5 36-37 1.0002144745609023E-4 9.573481399368636E-4 0.0 0.011881119079962718 5.715511283205156E-5 38-39 1.0002144745609023E-4 9.716369181448765E-4 0.0 0.019182684744257307 5.715511283205156E-5 40-41 1.0002144745609023E-4 0.0010716583656009667 0.0 0.025848399778295318 5.715511283205156E-5 42-43 1.0002144745609023E-4 0.001107380311120999 0.0 0.032206906080861054 5.715511283205156E-5 44-45 1.0716583656009668E-4 0.001250268093201128 0.0 0.039229840570099386 5.715511283205156E-5 46-47 1.1431022566410312E-4 0.0013431451515532116 0.0 0.04637422967410583 5.715511283205156E-5 48-49 1.28599003872116E-4 0.0013574339297612245 0.0 0.053847260676896574 5.715511283205156E-5 50-51 1.28599003872116E-4 0.0014503109881133084 0.0 0.06077017371867882 5.715511283205156E-5 52-53 1.28599003872116E-4 0.0014717441554253276 0.0 0.06780025259702116 7.144389104006445E-5 54-55 1.28599003872116E-4 0.0015003217118413534 0.0 0.07543760454920406 8.573266924807734E-5 56-57 1.28599003872116E-4 0.0015503324355693984 0.0 0.08263914876604256 8.573266924807734E-5 58-59 1.28599003872116E-4 0.001557476824673405 0.0 0.08931915257828857 8.573266924807734E-5 60-61 1.428877820801289E-4 0.0015931987701934373 0.0 0.0961063222270947 8.573266924807734E-5 62-63 1.428877820801289E-4 0.0016289207157134695 0.0 0.10265058264636459 1.0002144745609023E-4 64-65 1.428877820801289E-4 0.0018718299452496886 0.0 0.10888763433416222 1.0716583656009668E-4 66-67 1.428877820801289E-4 0.0018861187234577015 0.0 0.11501037579629575 1.1431022566410312E-4 68-69 1.428877820801289E-4 0.0019289850580817402 0.0 0.12121884992767734 1.1431022566410312E-4 70-71 1.428877820801289E-4 0.001943273836289753 0.0 0.12676289587238637 1.1431022566410312E-4 72-73 1.428877820801289E-4 0.0019504182253937594 0.0 0.13129958295343044 1.7146533849615468E-4 74-75 1.428877820801289E-4 0.001978995781809785 0.0 0.13504324284392982 1.7146533849615468E-4 76-77 1.428877820801289E-4 0.0020575840619538564 0.0 0.13862258178503706 1.7146533849615468E-4 78-79 1.428877820801289E-4 0.002100450396577895 0.0 0.14130172769903948 1.7146533849615468E-4 80-81 1.428877820801289E-4 0.0021075947856819014 0.0 0.14225907583897635 1.7860972760016112E-4 82-83 1.428877820801289E-4 0.0021290279529939202 0.0 0.14273060551984076 2.0004289491218046E-4 84-85 1.428877820801289E-4 0.0021433167312019333 0.0 0.14290921524744093 2.0004289491218046E-4 86-87 1.428877820801289E-4 0.002171894287617959 0.0 0.143023525473105 2.0004289491218046E-4 88 1.428877820801289E-4 0.002314782069698088 0.0 0.14303066986220903 2.0004289491218046E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7065 0.0 44.55155 1 GTATCAA 12410 0.0 34.428562 1 TCAACGC 14500 0.0 28.981178 4 ATCAACG 14685 0.0 28.616077 3 CAACGCA 15015 0.0 27.987154 5 AACGCAG 15490 0.0 27.315842 6 TATCAAC 15560 0.0 27.272701 2 CTTATAC 5555 0.0 24.94314 1 ACGCAGA 18050 0.0 23.32711 7 CGCAGAG 18535 0.0 22.716717 8 TATACAC 7680 0.0 21.649866 3 GTACATG 17965 0.0 20.605642 1 TACATGG 17860 0.0 20.194141 2 ACATGGG 18245 0.0 19.06525 3 GCAGAGT 22115 0.0 18.83358 9 TATGCCG 4250 0.0 18.673784 42-43 GTGGTAT 3810 0.0 18.563568 1 TTATACA 8405 0.0 18.20767 2 CTCGTAT 4245 0.0 17.864885 38-39 GAGTACT 12935 0.0 17.634882 12-13 >>END_MODULE