FastQCFastQC Report
Fri 27 May 2016
SRR2140208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7347449
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.043.916121
GTATCAA132400.032.8588871
TCAACGC150850.028.0412034
ATCAACG152850.027.7286593
CAACGCA154750.027.3076535
AACGCAG161700.026.2624366
TATCAAC163800.026.2555182
ACGCAGA187900.022.5783947
TACATGG205050.022.1895482
GTACATG210400.022.0992971
CGCAGAG194100.021.9214138
ACATGGG207050.021.2728043
GCAGAGT232950.018.140639
CATGGGG156600.017.8042984
GAGTACT124250.017.32317512-13
GTGGTAT44700.017.290181
CAGAGTA224400.016.85049410-11
AGAGTAC203300.016.69855110-11
TATCGCG3709.549694E-1015.7224587
GTACTTT140950.015.19761514-15
Produced by FastQC (version 0.11.5)