FastQCFastQC Report
Fri 27 May 2016
SRR2140207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9293285
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98250.039.0396421
CTTATAC75450.031.8621831
GTATCAA163650.031.8576131
TCAACGC181500.027.607444
ATCAACG184250.027.2403073
CAACGCA188450.026.5673275
AACGCAG196100.025.6153186
TATCAAC201600.025.3064482
TATACAC108100.024.9563223
TTATACA112050.022.304052
ACGCAGA227300.022.0992687
CGCAGAG234050.021.4265698
GTACATG252200.021.3939591
TACATGG250150.021.1888452
ACATGGG256050.020.1673323
TACACAT138950.019.415465
ACATCTC129200.019.3754378
ACACATC128550.019.183726
CATGGGG200700.018.18364
GCAGAGT284800.017.47779
Produced by FastQC (version 0.11.5)