##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9293285
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33608428020877	33.0	31.0	34.0	31.0	34.0
2	32.63163585319938	34.0	31.0	34.0	31.0	34.0
3	32.30285039143855	33.0	31.0	34.0	30.0	34.0
4	36.17264024508018	37.0	35.0	37.0	35.0	37.0
5	36.054371947056396	37.0	35.0	37.0	35.0	37.0
6	36.14221053158275	37.0	36.0	37.0	35.0	37.0
7	36.11990033664092	37.0	36.0	37.0	35.0	37.0
8	36.11892565438379	37.0	36.0	37.0	35.0	37.0
9	37.937618936683855	39.0	38.0	39.0	35.0	39.0
10-11	37.86383324088307	39.0	38.0	39.0	35.0	39.0
12-13	37.828754579247274	39.0	38.0	39.0	35.0	39.0
14-15	39.21584283705923	41.0	39.0	41.0	36.0	41.0
16-17	39.20027589813505	41.0	39.0	41.0	36.0	41.0
18-19	39.16143747878172	40.0	39.0	41.0	36.0	41.0
20-21	39.14932184925041	40.0	39.0	41.0	36.0	41.0
22-23	39.086827471657585	40.0	39.0	41.0	36.0	41.0
24-25	39.03265606422765	40.0	39.0	41.0	35.0	41.0
26-27	38.87714380313291	40.0	38.0	41.0	35.0	41.0
28-29	38.75727090188008	40.0	38.0	41.0	35.0	41.0
30-31	38.63124328738064	40.0	38.0	41.0	34.5	41.0
32-33	38.48139638986875	40.0	38.0	41.0	34.0	41.0
34-35	38.573089915458226	40.0	38.0	41.0	34.0	41.0
36-37	38.567056488368024	40.0	38.0	41.0	34.0	41.0
38-39	38.51466444955106	40.0	38.0	41.0	34.0	41.0
40-41	38.45436929524273	40.0	38.0	41.0	34.0	41.0
42-43	38.42404312606013	40.0	38.0	41.0	34.0	41.0
44-45	38.476404503295754	40.0	38.0	41.0	34.0	41.0
46-47	38.431599239649884	40.0	38.0	41.0	34.0	41.0
48-49	38.349631642307855	40.0	38.0	41.0	34.0	41.0
50-51	38.24819724371282	40.0	38.0	41.0	33.5	41.0
52-53	38.04956933763894	40.0	37.0	41.0	33.0	41.0
54-55	37.93707324660545	40.0	37.0	41.0	33.0	41.0
56-57	37.79272368789374	40.0	36.5	41.0	33.0	41.0
58-59	37.602428631947376	39.0	36.0	41.0	33.0	41.0
60-61	37.39581197314013	39.0	35.5	41.0	32.0	41.0
62-63	37.10982005962232	39.0	35.0	40.5	32.0	41.0
64-65	36.79589927106849	38.0	35.0	40.0	31.0	41.0
66-67	36.51004382439743	37.5	35.0	40.0	31.0	41.0
68-69	36.221711935674335	37.0	35.0	40.0	31.0	41.0
70-71	35.91260860130013	36.5	35.0	39.0	31.0	41.0
72-73	35.572246390045166	36.0	34.0	39.0	31.0	40.5
74-75	35.22539029575274	35.5	34.0	38.5	30.0	40.0
76-77	34.05578021008857	35.0	32.5	37.0	29.0	39.0
78-79	34.65499127157433	35.0	34.0	37.0	30.0	39.0
80-81	34.51577817312409	35.0	34.0	37.0	30.0	39.0
82-83	34.28824491037501	35.0	34.0	36.0	30.0	38.0
84-85	34.08828102329293	35.0	34.0	36.0	30.0	37.0
86-87	33.873078795394115	35.0	34.0	35.5	30.0	37.0
88-89	33.71365446295518	35.0	34.0	35.0	30.0	36.5
90-91	33.55310880314493	35.0	34.0	35.0	30.0	36.0
92-93	33.44755507863478	35.0	33.0	35.0	30.0	36.0
94-95	33.42488364804281	35.0	33.0	35.0	30.0	36.0
96-97	33.4446841984344	35.0	33.0	35.0	30.0	35.0
98-99	33.50865681101421	35.0	33.0	35.0	30.0	35.0
100	32.37214579528943	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	43.0
21	220.0
22	1022.0
23	3466.0
24	9106.0
25	19940.0
26	36308.0
27	59511.0
28	85847.0
29	115539.0
30	149351.0
31	188527.0
32	238170.0
33	306880.0
34	421446.0
35	608284.0
36	1008802.0
37	1901154.0
38	2582710.0
39	1487106.0
40	69847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12177932776193	19.819331915463692	13.486382909810684	24.572505846963693
2	15.266076527299013	20.63407072956441	40.441200286012965	23.658652457123612
3	18.64638822547678	25.08166918371706	30.093847331702406	26.17809525910375
4	12.801985519652092	17.286449301834605	37.684188099256616	32.22737707925669
5	13.566892654212154	37.52027404733633	35.42061822057539	13.492215077876121
6	31.455594012235714	36.83247635254918	18.102759142757378	13.609170492457725
7	27.72558896020083	31.843035051652883	22.13468111652661	18.29669487161967
8	25.201702089196658	36.46846082951292	20.642743658458766	17.687093422831644
9	25.61934773333649	16.55926833191923	20.63870848682678	37.182675447917504
10-11	24.913537032384138	26.337532960626948	28.291153235911736	20.457776771077178
12-13	25.373460514769537	23.54636708117743	28.576638938760624	22.503533465292413
14-15	22.365600538453304	25.17722742819143	25.77104866578395	26.68612336757132
16-17	22.425132770597266	28.516391136180587	26.498111270664786	22.560364822557364
18-19	22.529947030325907	27.714929838263114	27.88759294119318	21.867530190217796
20-21	23.125741690803228	26.825248069613156	27.82993376919034	22.219076470393272
22-23	22.82786464036521	26.954112268063255	27.630377733427245	22.58764535814428
24-25	22.717945422403123	27.039496211434983	27.81138585666263	22.431172509499262
26-27	22.717422807446276	27.17534818462991	27.771074468908918	22.336154539014892
28-29	22.600221281761563	27.117318994493605	27.83333771614633	22.449122007598504
30-31	22.9074085209288	27.086103467696343	27.631269148944554	22.3752188624303
32-33	22.690006922219894	27.024229502345236	27.719424190862668	22.566339384572203
34-35	22.587777070628555	27.240349804113865	27.919414115278073	22.252459009979496
36-37	22.798020072819693	27.11719702043724	27.653604436087797	22.431178470655265
38-39	22.505008445570887	27.135064452229052	27.79411539463003	22.565811707570028
40-41	22.778769315553955	26.97441106185941	27.728669682174328	22.518149940412307
42-43	22.720143405070463	27.018458398858424	27.663195550242182	22.598202645828934
44-45	22.59845357874161	27.205871934828824	27.555910841894494	22.639763644535073
46-47	22.83488017365758	27.037841092219594	27.272968005269522	22.854310728853307
48-49	22.708263544128606	27.140180383602036	27.303333538112728	22.848222534156623
50-51	22.602481021599196	27.2394747222607	27.403642318458783	22.754401937681322
52-53	22.801306515239514	27.206237988608656	27.393859832641553	22.59859566351028
54-55	22.727078004215436	27.14822564086386	27.279801938895233	22.84489441602547
56-57	22.83799862838326	27.41275666391832	27.068438939345075	22.68080576835335
58-59	22.608697352252168	27.585843266667005	27.32522366076794	22.48023572031289
60-61	22.82361785320767	27.724972156980115	26.994211099601795	22.45719889021042
62-63	22.666750966366607	27.912387346865188	26.945486048118244	22.47537563864996
64-65	22.675589872462627	27.749584279576233	26.980832913958608	22.593992934002536
66-67	22.73763393373635	27.64035300531072	26.928189115573442	22.69382394537949
68-69	22.693659076044888	27.49715666742974	27.097927309946247	22.711256946579127
70-71	22.72233980322422	27.245930765413384	27.15699754226056	22.874731889101838
72-73	22.773651874560677	27.286536027281542	27.066991175526084	22.872820922631696
74-75	22.646858740883513	27.405532727630494	27.104914791135148	22.842693740350846
76-77	22.79038739120562	27.341416351512436	26.998834943889477	22.869361313392464
78-79	22.660095600666025	27.37474006004436	27.024776661107257	22.940387678182354
80-81	22.715811180672663	27.362015946842	27.066949073213802	22.85522379927154
82-83	22.755110004734437	27.36618032368947	27.147261240114094	22.731448431461995
84-85	22.829021997023304	27.387802425928353	27.031301168679196	22.751874408369144
86-87	22.69958552294058	27.363523375922398	27.146628284374565	22.790262816762453
88-89	22.744150453887137	27.339133615924798	27.10335354242274	22.813362387765327
90-91	22.730982948830786	27.393997426568838	27.14423114536834	22.730788479232032
92-93	22.754616264734427	27.373988007177225	27.175245976538704	22.696149751549644
94-95	22.635865104390884	27.45212891928217	27.19629279322865	22.7157131830983
96-97	22.681590151917757	27.56083315594784	27.246467379165505	22.511109312968898
98-99	23.77486540011276	29.543423294575728	27.417261521317876	19.264449783993644
100	22.741861336632283	30.572864306757964	21.133623518238632	25.55165083837112
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1075.0
1	1011.0
2	1491.0
3	2203.0
4	2873.0
5	4547.0
6	6134.0
7	7263.0
8	8085.5
9	8112.0
10	7496.0
11	6801.0
12	7201.0
13	9329.5
14	14367.0
15	21518.5
16	27703.5
17	31747.5
18	33542.0
19	33221.0
20	31736.0
21	30359.0
22	30334.0
23	33168.5
24	39542.5
25	49382.0
26	62750.5
27	78382.0
28	96110.5
29	116516.5
30	136650.0
31	156095.5
32	176705.0
33	197098.0
34	217632.5
35	238174.5
36	260719.5
37	281454.0
38	298971.5
39	316657.5
40	335135.0
41	352472.5
42	365000.0
43	376594.5
44	392063.0
45	413568.5
46	428070.5
47	435445.5
48	435935.5
49	429018.0
50	423346.5
51	414452.5
52	398599.0
53	377447.0
54	356333.0
55	333717.0
56	310408.0
57	285051.5
58	255121.5
59	222354.0
60	188078.5
61	156241.5
62	128085.0
63	102301.5
64	79313.5
65	60987.0
66	47076.0
67	36412.5
68	27894.0
69	21036.0
70	15621.0
71	11210.0
72	7888.5
73	5469.5
74	3844.5
75	2684.0
76	1829.0
77	1143.5
78	682.0
79	427.0
80	285.5
81	205.5
82	156.5
83	106.5
84	65.5
85	42.0
86	26.0
87	20.0
88	13.5
89	8.5
90	6.0
91	4.5
92	3.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.62699680468209E-4
20-21	0.0
22-23	5.00420703147049E-4
24-25	0.00666806701455786
26-27	0.028683822765415538
28-29	0.02748022794636286
30-31	0.02900411523877294
32-33	0.05023448713900795
34-35	0.023942063140875977
36-37	0.01960242587122485
38-39	0.01519299733929742
40-41	0.007453812210845168
42-43	0.010736051700876098
44-45	0.004845437623774721
46-47	0.0038401097514829986
48-49	0.008084268795272992
50-51	0.0022138415110862213
52-53	0.0020997590186424293
54-55	0.0011172687689801261
56-57	0.006547763549143955
58-59	0.004588141310353608
60-61	0.007583703973731088
62-63	0.006084467162011914
64-65	0.001284526230025617
66-67	0.004249420335482442
68-69	0.011373335821480587
70-71	0.015061231902176344
72-73	0.004680581286916171
74-75	0.0016430987495614476
76-77	0.0023844214708842043
78-79	0.002710094751256746
80-81	0.003506673367067252
82-83	0.0032689262871980988
84-85	0.0042061796159077285
86-87	2.9042282804746842E-5
88-89	2.2091216399812135E-4
90-91	0.0037695762734492513
92-93	5.095923534982831E-4
94-95	0.002083893040664887
96-97	5.563965091365076E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	207.0
20-21	659.0
22-23	1269.0
24-25	2788.0
26-27	4861.0
28-29	11544.0
30-31	25950.0
32-33	32727.0
34-35	45153.0
36-37	70779.0
38-39	82342.0
40-41	79756.0
42-43	88418.0
44-45	98244.0
46-47	97291.0
48-49	92498.0
50-51	85105.0
52-53	83205.0
54-55	89474.0
56-57	93389.0
58-59	93821.0
60-61	95137.0
62-63	93234.0
64-65	98017.0
66-67	103441.0
68-69	101248.0
70-71	91856.0
72-73	84801.0
74-75	85521.0
76-77	86738.0
78-79	92910.0
80-81	92186.0
82-83	88544.0
84-85	90093.0
86-87	95036.0
88-89	102404.0
90-91	110754.0
92-93	114879.0
94-95	141592.0
96-97	335368.0
98-99	595797.0
100-101	5414249.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.46226087229212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.04259655768212	76.95243876631181
2	6.936547988201071	11.85626147441626
3	1.6724112648214735	4.287841433997984
4	0.6108449148537113	2.0881674986296375
5	0.2941158395934237	1.2567902305003198
6	0.15577644298433363	0.7987804204850915
7	0.08885092692415528	0.5315380766875986
8	0.04985763771802438	0.3408757152907224
9	0.03667197416128226	0.28206628402261147
>10	0.11015061936978601	1.4277267714885926
>50	0.001866308520616239	0.10677106689534299
>100	2.6913261294872655E-4	0.039190652580784115
>500	2.01963518516972E-5	0.011650674761673835
>1k	2.0196205119223027E-5	0.01990093393160352
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.304183074122875E-5	0.0	1.0760457685307187E-5	0.0
3	0.0	4.304183074122875E-5	0.0	1.0760457685307187E-5	0.0
4	0.0	1.0760457685307187E-4	0.0	1.0760457685307187E-5	0.0
5	0.0	1.0760457685307187E-4	0.0	1.0760457685307187E-5	0.0
6	0.0	1.0760457685307187E-4	0.0	1.0760457685307187E-5	0.0
7	0.0	1.0760457685307187E-4	0.0	1.0760457685307187E-5	0.0
8	1.0760457685307187E-5	1.1836503453837906E-4	0.0	1.0760457685307187E-5	0.0
9	2.1520915370614374E-5	2.0444869602083654E-4	0.0	1.0760457685307187E-5	1.0760457685307187E-5
10-11	3.228137305592156E-5	2.0444869602083654E-4	0.0	1.0760457685307187E-5	1.0760457685307187E-5
12-13	5.918251726918953E-5	2.0444869602083654E-4	0.0	2.1520915370614374E-5	1.614068652796078E-5
14-15	7.532320379715031E-5	3.3895441708717635E-4	0.0	4.304183074122875E-5	4.842205958388234E-5
16-17	8.60836614824575E-5	3.6047533245779076E-4	0.0	4.304183074122875E-5	5.3802288426535935E-5
18-19	1.0222434801041827E-4	4.142776208843267E-4	0.0	6.456274611184312E-5	6.456274611184312E-5
20-21	1.1298480569572547E-4	4.62699680468209E-4	0.0	8.07034326398039E-5	6.456274611184312E-5
22-23	1.1836503453837906E-4	4.842205958388234E-4	0.0	1.0760457685307187E-4	6.456274611184312E-5
24-25	1.2374526338103264E-4	5.380228842653593E-4	0.0	1.0760457685307187E-4	6.456274611184312E-5
26-27	1.72167322964915E-4	5.810647150065881E-4	0.0	1.3988594990899344E-4	6.456274611184312E-5
28-29	1.72167322964915E-4	5.810647150065881E-4	0.0	5.057415112094377E-4	6.456274611184312E-5
30-31	1.9906846717818297E-4	6.8866929185966E-4	0.0	0.0015387454489989277	6.456274611184312E-5
32-33	2.0444869602083654E-4	6.8866929185966E-4	0.0	0.003599373095735254	6.456274611184312E-5
34-35	2.1520915370614374E-4	8.124145552406926E-4	0.0	0.006294867745904704	6.456274611184312E-5
36-37	2.367300690767581E-4	9.415400474643788E-4	0.0	0.010227815029884482	6.456274611184312E-5
38-39	2.367300690767581E-4	9.79201649362954E-4	0.0	0.016883158108246976	6.456274611184312E-5
40-41	2.474905267620653E-4	0.0010007225647335684	0.0	0.02359768370387866	7.532320379715031E-5
42-43	2.474905267620653E-4	0.0010491446243174507	0.0	0.03007009900159093	7.532320379715031E-5
44-45	2.474905267620653E-4	0.0012213119472823657	0.0	0.037188141760421636	7.532320379715031E-5
46-47	2.474905267620653E-4	0.0013342967529780911	0.0	0.0442416217731405	8.07034326398039E-5
48-49	2.5287075560471886E-4	0.0013342967529780911	0.0	0.05132738315891528	8.60836614824575E-5
50-51	2.582509844473725E-4	0.0015118443047856599	0.0	0.05842928523121803	8.60836614824575E-5
52-53	2.582509844473725E-4	0.0015279849913136206	0.0	0.06531059792097196	9.684411916776468E-5
54-55	2.582509844473725E-4	0.0015387454489989277	0.0	0.0723102756452643	1.0760457685307187E-4
56-57	2.636312132900261E-4	0.001549505906684235	0.0	0.07892257689288557	1.0760457685307187E-4
58-59	2.690114421326797E-4	0.0015548861355268884	0.0	0.08589535347296462	1.0760457685307187E-4
60-61	2.690114421326797E-4	0.0015710268220548492	0.0	0.0926206395262816	1.1836503453837906E-4
62-63	2.7439167097533326E-4	0.0015925477374254637	0.0	0.09917375825663369	1.1836503453837906E-4
64-65	2.7977189981798683E-4	0.0017700952892330322	0.0	0.10530183890841613	1.1836503453837906E-4
66-67	2.7977189981798683E-4	0.001786235975760993	0.0	0.11156980551010756	1.1836503453837906E-4
68-69	2.7977189981798683E-4	0.001850798721872836	0.0	0.11704687847192893	1.1836503453837906E-4
70-71	2.851521286606404E-4	0.001893840552614065	0.0	0.12235178411078537	1.3988594990899342E-4
72-73	2.9053235750329403E-4	0.001893840552614065	0.0	0.12688731702514233	1.5064640759430062E-4
74-75	2.9053235750329403E-4	0.00193150215451264	0.0	0.13049207034972027	1.5064640759430062E-4
76-77	2.9053235750329403E-4	0.0019691637564112153	0.0	0.13421518870883653	1.5064640759430062E-4
78-79	2.9053235750329403E-4	0.0019906846717818298	0.0	0.13681921946868086	1.5064640759430062E-4
80-81	2.9053235750329403E-4	0.0019906846717818298	0.0	0.13768543631234811	1.5064640759430062E-4
82-83	2.9053235750329403E-4	0.002001445129467137	0.0	0.13816965690818694	1.5064640759430062E-4
84-85	2.9053235750329403E-4	0.002012205587152444	0.0	0.1383472044599945	1.5064640759430062E-4
86-87	2.9053235750329403E-4	0.0020337265025230583	0.0	0.1384601892656902	1.5064640759430062E-4
88	2.9053235750329403E-4	0.0021628519947467445	0.0	0.13846556949453287	1.5064640759430062E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9825	0.0	39.039642	1
CTTATAC	7545	0.0	31.862183	1
GTATCAA	16365	0.0	31.857613	1
TCAACGC	18150	0.0	27.60744	4
ATCAACG	18425	0.0	27.240307	3
CAACGCA	18845	0.0	26.567327	5
AACGCAG	19610	0.0	25.615318	6
TATCAAC	20160	0.0	25.306448	2
TATACAC	10810	0.0	24.956322	3
TTATACA	11205	0.0	22.30405	2
ACGCAGA	22730	0.0	22.099268	7
CGCAGAG	23405	0.0	21.426569	8
GTACATG	25220	0.0	21.393959	1
TACATGG	25015	0.0	21.188845	2
ACATGGG	25605	0.0	20.167332	3
TACACAT	13895	0.0	19.41546	5
ACATCTC	12920	0.0	19.375437	8
ACACATC	12855	0.0	19.18372	6
CATGGGG	20070	0.0	18.1836	4
GCAGAGT	28480	0.0	17.4777	9
>>END_MODULE