FastQCFastQC Report
Fri 27 May 2016
SRR2140206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7283695
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76550.042.412531
GTATCAA128750.033.047281
TCAACGC144700.028.3523464
ATCAACG146150.028.0992093
CAACGCA149650.027.4420265
AACGCAG153050.026.8324036
TATCAAC157150.026.3680172
ACGCAGA178500.022.914517
CGCAGAG183050.022.344938
GTACATG197200.021.7014521
TACATGG193400.021.2767812
ACATGGG196700.020.3968283
GCAGAGT218950.018.6059849
CATGGGG154400.017.6696784
GAGTACT118050.017.09760912-13
GTGGTAT42650.016.9807171
CAGAGTA216400.016.72403110-11
AGAGTAC193550.016.71058810-11
TGGTATC42000.016.3617422
AAAGGCG32000.016.13831194
Produced by FastQC (version 0.11.5)