##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7283695
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.191147075763055	33.0	31.0	34.0	31.0	34.0
2	32.47848777303278	34.0	31.0	34.0	31.0	34.0
3	32.272532279289564	33.0	31.0	34.0	30.0	34.0
4	36.08722042864233	37.0	35.0	37.0	35.0	37.0
5	35.92873232610646	37.0	35.0	37.0	35.0	37.0
6	36.02671844441592	37.0	35.0	37.0	35.0	37.0
7	35.99511470483045	37.0	35.0	37.0	35.0	37.0
8	35.97269504008611	37.0	35.0	37.0	35.0	37.0
9	37.76424671818356	39.0	38.0	39.0	35.0	39.0
10-11	37.69002731443313	39.0	37.0	39.0	35.0	39.0
12-13	37.66172148888717	39.0	37.0	39.0	35.0	39.0
14-15	38.996789472925485	40.0	38.0	41.0	35.5	41.0
16-17	38.977223785455045	40.0	38.0	41.0	35.5	41.0
18-19	38.94588186353218	40.0	38.0	41.0	35.0	41.0
20-21	38.93584882421743	40.0	38.0	41.0	35.0	41.0
22-23	38.85039799285987	40.0	38.0	41.0	35.0	41.0
24-25	38.78626891207007	40.0	38.0	41.0	35.0	41.0
26-27	38.641863537363236	40.0	38.0	41.0	34.0	41.0
28-29	38.51991974810201	40.0	38.0	41.0	34.0	41.0
30-31	38.376891741269965	40.0	38.0	41.0	34.0	41.0
32-33	38.220881005995025	40.0	38.0	41.0	34.0	41.0
34-35	38.32144806642884	40.0	38.0	41.0	34.0	41.0
36-37	38.318291450263274	40.0	38.0	41.0	34.0	41.0
38-39	38.25359157784973	40.0	38.0	41.0	34.0	41.0
40-41	38.23325127814927	40.0	38.0	41.0	33.0	41.0
42-43	38.30522976907395	40.0	38.0	41.0	34.0	41.0
44-45	38.26334363745313	40.0	38.0	41.0	33.0	41.0
46-47	38.179451134530396	40.0	38.0	41.0	33.0	41.0
48-49	38.08449125439542	40.0	37.0	41.0	33.0	41.0
50-51	37.97568897486545	40.0	37.0	41.0	33.0	41.0
52-53	37.826972732262064	40.0	37.0	41.0	33.0	41.0
54-55	37.66397570189967	40.0	36.5	41.0	33.0	41.0
56-57	37.55692989068015	39.0	36.0	41.0	32.0	41.0
58-59	37.35112234346664	39.0	36.0	41.0	32.0	41.0
60-61	37.146022953834056	39.0	35.0	41.0	32.0	41.0
62-63	36.88377060365441	38.5	35.0	40.0	31.0	41.0
64-65	36.57365054787018	38.0	35.0	40.0	31.0	41.0
66-67	36.2833854080572	37.5	35.0	40.0	31.0	41.0
68-69	35.99686980513607	37.0	34.5	39.5	30.5	41.0
70-71	35.6817206710101	36.5	34.0	39.0	30.0	41.0
72-73	35.21746779577221	36.0	34.0	39.0	30.0	40.5
74-75	34.975420377436656	35.5	34.0	38.5	30.0	40.0
76-77	33.75176870412895	34.5	32.5	36.5	28.5	39.0
78-79	34.45569915516316	35.0	34.0	37.0	29.5	39.0
80-81	34.3658597753663	35.0	34.0	36.5	30.0	39.0
82-83	34.127158055863575	35.0	34.0	36.0	30.0	38.0
84-85	33.90740209468875	35.0	34.0	36.0	30.0	37.0
86-87	33.71752836735722	35.0	33.5	35.5	29.5	37.0
88-89	33.53860107857148	35.0	33.0	35.0	29.0	36.0
90-91	33.38479561791394	35.0	33.0	35.0	29.0	36.0
92-93	33.301859002290364	35.0	33.0	35.0	29.0	36.0
94-95	33.2589130443677	35.0	33.0	35.0	29.0	36.0
96-97	33.308843647485915	35.0	33.0	35.0	29.0	35.0
98-99	33.39994118276605	35.0	33.0	35.0	30.0	35.0
100	32.209078324334854	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	48.0
21	218.0
22	1020.0
23	3330.0
24	8817.0
25	18691.0
26	34232.0
27	54449.0
28	78522.0
29	105518.0
30	134606.0
31	168829.0
32	211136.0
33	269411.0
34	364912.0
35	512576.0
36	816418.0
37	1460779.0
38	1901864.0
39	1089469.0
40	48840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22594438674327	19.8179907313527	13.52895748655044	24.427107395353595
2	15.350313268196981	20.89317303923352	40.22860649711444	23.527907195455054
3	18.688577706782066	25.21257411245254	30.109470536588915	25.989377644176482
4	12.89105323602924	17.254346866528596	38.09908295171613	31.755516945726036
5	13.502254007066469	37.57104052270173	35.453928260312935	13.472777209918869
6	31.299141438514383	37.10948907113766	18.156224279023213	13.435145211324748
7	27.53546105376461	32.08324895537224	22.46803305190566	17.913256938957495
8	25.103687070916614	36.409981472315906	20.731332105476685	17.75499935129079
9	25.65961369881633	16.547260696665635	20.920439419827435	36.8726861846906
10-11	24.74413741926316	26.484133396579622	28.466080746104826	20.30564843805239
12-13	25.1723198184438	23.803509070602217	28.756283726872145	22.267887384081845
14-15	22.271182414969324	25.40873141997297	26.141765958074853	26.178320206982857
16-17	22.14619090997083	28.626768419051047	26.878878096899996	22.348162574078128
18-19	22.213121224872815	27.8676070318705	28.314900610198535	21.60437113305815
20-21	22.916331985271	27.095105536886628	28.19760440892451	21.790958068917867
22-23	22.614008700193747	27.029866124617	28.028220874986086	22.327904300203166
24-25	22.390732988988596	27.097227145433028	28.194480934573356	22.31755893100502
26-27	22.392406919950247	27.275181138075766	28.0843315263895	22.248080415584486
28-29	22.29890718598587	27.380540132282764	28.111472983107884	22.209079698623484
30-31	22.590684701460543	27.23130918600797	27.98344380963453	22.194562302896962
32-33	22.390466860974826	27.31271539839791	27.97301324166021	22.323804498967053
34-35	22.444655899466888	27.409291560437556	27.993661155472843	22.15239138462272
36-37	22.50235486314883	27.28188173766068	27.942170654323238	22.273592744867248
38-39	22.251537252542406	27.485948935380566	28.044870623363988	22.217643188713037
40-41	22.567469849181183	27.300805286726685	27.869559651045968	22.262165213046163
42-43	22.406300773709276	27.387932489010492	27.769407533963914	22.436359203316318
44-45	22.431451492435013	27.530407629593444	27.657760907296435	22.380379970675108
46-47	22.5317333742871	27.432240535840414	27.437123072047857	22.59890301782462
48-49	22.472430425924607	27.489757375304553	27.490001802838233	22.547810395932608
50-51	22.422261325596395	27.632434806090085	27.463538782807383	22.48176508550614
52-53	22.61818548276051	27.57348546360401	27.321522981296535	22.48680607233895
54-55	22.566130054620658	27.42205141409052	27.392801409444928	22.619017121843896
56-57	22.566772650641493	27.603846926615596	27.307132027530923	22.522248395211992
58-59	22.479553610222972	27.681650441336952	27.396200838441164	22.442595109998912
60-61	22.585019911504073	27.6340407079991	27.366831762490236	22.414107618006586
62-63	22.578643830828383	27.728820679238993	27.26931142957008	22.42322406036255
64-65	22.487792228733518	27.6795513474234	27.31483700760827	22.517819416234815
66-67	22.57338015548628	27.698781206315225	27.1477067092012	22.5801319289973
68-69	22.548457259221763	27.631960693221735	27.31421936153118	22.505362686025325
70-71	22.492200056726862	27.416708885698416	27.33356684175863	22.7575242158161
72-73	22.581061844098166	27.47657417935196	27.262543897650932	22.679820078898942
74-75	22.414766401802844	27.598771185025857	27.298288658720114	22.688173754451185
76-77	22.54454681882795	27.58334570343341	27.183544653198766	22.688562824539872
78-79	22.459959536580975	27.610737326407254	27.154220581850147	22.775082555161628
80-81	22.50355683101625	27.621420941604914	27.1895206759213	22.68550155145753
82-83	22.542259855576905	27.60844433219578	27.33283101649897	22.516464795728343
84-85	22.660266190825723	27.54445927225878	27.22028089158275	22.574993645332746
86-87	22.451910196948084	27.59277016053615	27.28506199722844	22.67025764528733
88-89	22.48721804223969	27.566909441071164	27.314241279036445	22.631631237652705
90-91	22.49393915650013	27.621396230912644	27.306557065643762	22.578107546943464
92-93	22.560437761959015	27.59372406275937	27.32633069708907	22.519507478192544
94-95	22.39505518995837	27.702498784618168	27.332840779421087	22.569605246002382
96-97	22.40733917874906	27.824005628893183	27.45016647119012	22.318488721167643
98-99	23.509543465162047	29.7604455438823	27.653634631334047	19.076376359621605
100	30.11187425389794	41.347720061694574	28.54040568440749	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1373.0
1	1173.5
2	1412.5
3	1994.5
4	2629.5
5	4113.5
6	5496.0
7	6481.0
8	7188.5
9	7261.0
10	6709.5
11	6079.5
12	6468.5
13	8375.5
14	12935.5
15	19114.5
16	24399.5
17	28015.0
18	29685.0
19	29504.0
20	28484.0
21	27586.0
22	27850.5
23	30674.0
24	36506.0
25	45568.5
26	58265.0
27	72432.0
28	86365.5
29	102672.0
30	118487.5
31	132889.0
32	149375.5
33	166505.0
34	183314.5
35	199803.0
36	216853.5
37	232158.5
38	244336.0
39	256395.5
40	270245.5
41	283477.5
42	292299.0
43	300311.0
44	309694.5
45	317877.5
46	323357.0
47	327298.0
48	329482.5
49	327996.5
50	323670.0
51	315883.5
52	302846.5
53	285934.5
54	268866.5
55	250737.0
56	230980.5
57	210301.0
58	188866.0
59	164860.5
60	140804.5
61	118077.0
62	95759.0
63	75637.0
64	58567.0
65	45892.5
66	35669.5
67	27109.0
68	20604.0
69	15723.0
70	11536.5
71	8241.0
72	5881.5
73	4131.0
74	2843.0
75	1947.0
76	1324.0
77	847.5
78	526.5
79	330.0
80	225.5
81	157.0
82	111.5
83	76.5
84	59.0
85	48.0
86	33.5
87	21.5
88	14.5
89	11.0
90	7.0
91	3.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005258512165080486
22-23	0.004709711184170373
24-25	0.0033990172239532794
26-27	0.01590855903143527
28-29	0.0202905968136752
30-31	0.03806399984138279
32-33	0.022570193370425134
34-35	0.011769051290398845
36-37	0.006755112867548898
38-39	0.002987255664451763
40-41	0.007446937907786342
42-43	0.009319463699937318
44-45	0.005895302608414615
46-47	0.0022984724600909417
48-49	0.010309334850899023
50-51	0.00468010438205287
52-53	2.2697272778926217E-5
54-55	5.964671252173376E-4
56-57	6.7308152038439E-4
58-59	5.559404507909702E-4
60-61	0.001164865285706984
62-63	0.0010902593662899499
64-65	0.0
66-67	2.546282796738458E-4
68-69	6.74128408145635E-4
70-71	3.2048970827424305E-4
72-73	7.603301097045289E-4
74-75	3.373572557111772E-4
76-77	0.0
78-79	2.6642865706635406E-5
80-81	4.8643267213320903E-4
82-83	0.0019279676561953047
84-85	0.0
86-87	6.488318769408536E-4
88-89	3.5346096076229876E-4
90-91	0.008680509146369617
92-93	7.722635410718958E-4
94-95	4.042848534477513E-5
96-97	2.1825424457736934E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	520.0
22-23	1003.0
24-25	2176.0
26-27	3799.0
28-29	8735.0
30-31	19606.0
32-33	25905.0
34-35	36407.0
36-37	55666.0
38-39	63799.0
40-41	64085.0
42-43	69651.0
44-45	81011.0
46-47	80790.0
48-49	75711.0
50-51	69008.0
52-53	68730.0
54-55	74585.0
56-57	77667.0
58-59	76136.0
60-61	73769.0
62-63	71799.0
64-65	75727.0
66-67	79444.0
68-69	79717.0
70-71	77224.0
72-73	72501.0
74-75	73934.0
76-77	74818.0
78-79	79145.0
80-81	79156.0
82-83	76638.0
84-85	77196.0
86-87	82058.0
88-89	88106.0
90-91	93555.0
92-93	98425.0
94-95	119501.0
96-97	267913.0
98-99	1512760.0
100-101	3075149.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.46218979205244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.0125265043265	78.69142340071282
2	6.268475623879246	10.839722581974033
3	1.4827795550102785	3.846131019152213
4	0.5514468434593758	1.9071720655765116
5	0.2580846724805698	1.1157282967217361
6	0.1388786322054421	0.720465039348455
7	0.07380348599954771	0.4466847709665585
8	0.05661092921590108	0.3915763924935749
9	0.035296335398124525	0.2746618605140923
>10	0.12030535881701418	1.5755257939777532
>50	0.0013518617867861883	0.07736390296382845
>100	3.652996691433639E-4	0.05532726071307057
>500	2.496600565802219E-5	0.0119155576679346
>1k	4.9931746380092057E-5	0.04630205721743951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.745859072901872E-5	0.0	0.0	0.0
3	0.0	2.745859072901872E-5	0.0	0.0	0.0
4	0.0	5.491718145803744E-5	0.0	1.372929536450936E-5	0.0
5	0.0	5.491718145803744E-5	0.0	1.372929536450936E-5	0.0
6	8.237577218705616E-5	5.491718145803744E-5	0.0	1.372929536450936E-5	4.118788609352808E-5
7	8.237577218705616E-5	5.491718145803744E-5	0.0	1.372929536450936E-5	5.491718145803744E-5
8	8.237577218705616E-5	6.86464768225468E-5	0.0	1.372929536450936E-5	5.491718145803744E-5
9	8.237577218705616E-5	1.7848083973862167E-4	0.0	1.372929536450936E-5	6.86464768225468E-5
10-11	8.924041986931083E-5	1.7848083973862167E-4	0.0	4.118788609352808E-5	6.86464768225468E-5
12-13	1.0983436291607488E-4	1.8534548742087635E-4	0.0	4.118788609352808E-5	6.86464768225468E-5
14-15	1.0983436291607488E-4	1.990747827853857E-4	0.0	4.805253377578276E-5	8.237577218705616E-5
16-17	1.4415760132734828E-4	2.0593943046764038E-4	0.0	5.491718145803744E-5	8.237577218705616E-5
18-19	1.5102224900960295E-4	2.0593943046764038E-4	0.0	8.237577218705616E-5	9.610506755156553E-5
20-21	1.5102224900960295E-4	2.3339802119665912E-4	0.0	9.610506755156551E-5	1.0983436291607488E-4
22-23	1.5102224900960295E-4	2.6085661192567784E-4	0.0	1.372929536450936E-4	1.1669901059832956E-4
24-25	1.647515443741123E-4	2.9517985033695126E-4	0.0	1.7848083973862167E-4	1.4415760132734828E-4
26-27	1.647515443741123E-4	3.295030887482246E-4	0.0	2.6085661192567784E-4	1.5102224900960295E-4
28-29	1.647515443741123E-4	3.295030887482246E-4	0.0	6.109536437206665E-4	1.5102224900960295E-4
30-31	1.647515443741123E-4	3.63826327159498E-4	0.0	0.0018328609311619995	1.5102224900960295E-4
32-33	1.647515443741123E-4	3.706909748417527E-4	0.0	0.004166841143128591	1.647515443741123E-4
34-35	1.8534548742087635E-4	4.256081562997901E-4	0.0	0.006933294159077227	1.647515443741123E-4
36-37	1.9221013510313103E-4	4.94254633122337E-4	0.0	0.011546337401552371	1.647515443741123E-4
38-39	1.9221013510313103E-4	5.285778715336104E-4	0.0	0.0181158052334701	1.7848083973862167E-4
40-41	1.9221013510313103E-4	5.834950529916478E-4	0.0	0.0250834226309586	1.7848083973862167E-4
42-43	1.9221013510313103E-4	6.384122344496852E-4	0.0	0.032002987494671314	1.8534548742087635E-4
44-45	1.990747827853857E-4	7.207880066367414E-4	0.0	0.039567829240515974	1.9221013510313103E-4
46-47	2.0593943046764038E-4	7.825698357770335E-4	0.0	0.04747590337047336	1.990747827853857E-4
48-49	2.0593943046764038E-4	7.894344834592881E-4	0.0	0.055994931144151425	2.0593943046764038E-4
50-51	2.0593943046764038E-4	8.58080960281835E-4	0.0	0.06370393049132343	2.0593943046764038E-4
52-53	2.0593943046764038E-4	8.786749033285991E-4	0.0	0.07133741871399063	2.1280407814989506E-4
54-55	2.2653337351440442E-4	8.924041986931085E-4	0.0	0.0794171090360044	2.1966872583214977E-4
56-57	2.471273165611685E-4	9.061334940576178E-4	0.0	0.0879430014573647	2.2653337351440445E-4
58-59	2.471273165611685E-4	9.061334940576178E-4	0.0	0.09633160092507992	2.3339802119665912E-4
60-61	2.471273165611685E-4	9.473213801511459E-4	0.0	0.10426713364576634	2.471273165611685E-4
62-63	2.471273165611685E-4	9.747799708801645E-4	0.0	0.11144755512140472	2.471273165611685E-4
64-65	2.471273165611685E-4	0.0010571557430672208	0.0	0.11807194013478049	2.471273165611685E-4
66-67	2.471273165611685E-4	0.00107088503843173	0.0	0.12463454331901597	2.471273165611685E-4
68-69	2.6085661192567784E-4	0.0010983436291607488	0.0	0.13149919100127064	2.471273165611685E-4
70-71	2.6085661192567784E-4	0.0011189375722075127	0.0	0.1374302465987387	2.471273165611685E-4
72-73	2.6772125960793254E-4	0.001132666867572022	0.0	0.14233846969155078	2.471273165611685E-4
74-75	2.745859072901872E-4	0.001180719401347805	0.0	0.14644352900553909	2.5399196424342313E-4
76-77	2.745859072901872E-4	0.001263095173534861	0.0	0.15017103269700338	2.6772125960793254E-4
78-79	2.745859072901872E-4	0.0013180123549928985	0.0	0.1526491705102973	2.745859072901872E-4
80-81	2.745859072901872E-4	0.001331741650357408	0.0	0.15340428175534532	2.8145055497244185E-4
82-83	2.745859072901872E-4	0.0013866588318154453	0.0	0.15397404751297247	2.8831520265469655E-4
84-85	2.8831520265469655E-4	0.0013866588318154453	0.0	0.15414566370502883	2.8831520265469655E-4
86-87	3.020444980192059E-4	0.0014003881271799547	0.0	0.15430355060172068	2.8831520265469655E-4
88	3.020444980192059E-4	0.0014278467179089733	0.0	0.1543172798970852	2.8831520265469655E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7655	0.0	42.41253	1
GTATCAA	12875	0.0	33.04728	1
TCAACGC	14470	0.0	28.352346	4
ATCAACG	14615	0.0	28.099209	3
CAACGCA	14965	0.0	27.442026	5
AACGCAG	15305	0.0	26.832403	6
TATCAAC	15715	0.0	26.368017	2
ACGCAGA	17850	0.0	22.91451	7
CGCAGAG	18305	0.0	22.34493	8
GTACATG	19720	0.0	21.701452	1
TACATGG	19340	0.0	21.276781	2
ACATGGG	19670	0.0	20.396828	3
GCAGAGT	21895	0.0	18.605984	9
CATGGGG	15440	0.0	17.669678	4
GAGTACT	11805	0.0	17.097609	12-13
GTGGTAT	4265	0.0	16.980717	1
CAGAGTA	21640	0.0	16.724031	10-11
AGAGTAC	19355	0.0	16.710588	10-11
TGGTATC	4200	0.0	16.361742	2
AAAGGCG	3200	0.0	16.138311	94
>>END_MODULE