FastQCFastQC Report
Fri 27 May 2016
SRR2140205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8328430
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82450.042.907061
GTATCAA135900.032.9089431
TCAACGC157400.027.554314
ATCAACG157800.027.4584853
CAACGCA162350.026.7394375
AACGCAG167600.025.9262926
TATCAAC172050.025.803722
ACGCAGA198950.021.8202937
CGCAGAG201850.021.4458738
TACATGG210050.021.1160742
GTACATG217100.020.902411
ACATGGG216000.020.0219923
CATGGGG167250.018.2844244
AGACCGA136650.018.19796288-89
CTCTTAT124050.018.07865362-63
ACCGAGG141650.017.94501790-91
GACCGAG139000.017.79349190-91
CGAGACC136850.017.75306986-87
GCAGAGT245100.017.5612169
GAGACCG138900.017.4883588-89