##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8328430
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13852202636031	33.0	31.0	34.0	30.0	34.0
2	32.427381991563834	34.0	31.0	34.0	31.0	34.0
3	32.217659750997484	33.0	31.0	34.0	30.0	34.0
4	36.05142697963482	37.0	35.0	37.0	35.0	37.0
5	35.88361491901835	37.0	35.0	37.0	35.0	37.0
6	35.98631110545445	37.0	35.0	37.0	35.0	37.0
7	35.95043195416183	37.0	35.0	37.0	35.0	37.0
8	35.92770197984494	37.0	35.0	37.0	35.0	37.0
9	37.71384018356401	39.0	37.0	39.0	35.0	39.0
10-11	37.634442265829215	39.0	37.0	39.0	35.0	39.0
12-13	37.60631871793363	39.0	37.0	39.0	35.0	39.0
14-15	38.92364791443285	40.0	38.0	41.0	35.5	41.0
16-17	38.897754798923685	40.0	38.0	41.0	35.0	41.0
18-19	38.86702343659009	40.0	38.0	41.0	35.0	41.0
20-21	38.86044196826951	40.0	38.0	41.0	35.0	41.0
22-23	38.76820139350744	40.0	38.0	41.0	34.5	41.0
24-25	38.702667778943706	40.0	38.0	41.0	34.0	41.0
26-27	38.5521461345442	40.0	38.0	41.0	34.0	41.0
28-29	38.420401036846286	40.0	38.0	41.0	34.0	41.0
30-31	38.27050984183326	40.0	38.0	41.0	34.0	41.0
32-33	38.11337851283301	40.0	38.0	41.0	33.5	41.0
34-35	38.212842070932496	40.0	38.0	41.0	34.0	41.0
36-37	38.20714732901784	40.0	38.0	41.0	33.5	41.0
38-39	38.13076262605329	40.0	38.0	41.0	33.0	41.0
40-41	38.121434668020925	40.0	38.0	41.0	33.0	41.0
42-43	38.19412013868898	40.0	38.0	41.0	33.0	41.0
44-45	38.15107656536044	40.0	38.0	41.0	33.0	41.0
46-47	38.06389006202171	40.0	37.0	41.0	33.0	41.0
48-49	37.96365180335122	40.0	37.0	41.0	33.0	41.0
50-51	37.848612350471356	40.0	37.0	41.0	33.0	41.0
52-53	37.69914536787849	40.0	37.0	41.0	32.5	41.0
54-55	37.536447324791496	39.5	36.0	41.0	32.0	41.0
56-57	37.42702866776514	39.0	36.0	41.0	32.0	41.0
58-59	37.221974594374416	39.0	35.5	41.0	31.5	41.0
60-61	37.02340212499817	39.0	35.0	40.5	31.0	41.0
62-63	36.762678405347245	38.5	35.0	40.0	31.0	41.0
64-65	36.46328937718725	38.0	35.0	40.0	31.0	41.0
66-67	36.184879818054284	37.5	35.0	40.0	30.0	41.0
68-69	35.90473845793411	37.0	34.0	39.5	30.0	41.0
70-71	35.5974458060269	36.5	34.0	39.0	30.0	41.0
72-73	35.12885352962529	36.0	34.0	39.0	29.0	40.0
74-75	34.893420642692504	35.5	34.0	38.5	29.0	40.0
76-77	33.66868381475608	34.5	32.0	36.5	28.0	39.0
78-79	34.392269476961445	35.0	33.5	37.0	29.5	39.0
80-81	34.29498329824794	35.0	34.0	36.5	30.0	39.0
82-83	34.04998788469413	35.0	34.0	36.0	29.0	38.0
84-85	33.83330790830709	35.0	33.5	36.0	29.0	37.0
86-87	33.64646084912549	35.0	33.0	35.5	29.0	37.0
88-89	33.45976636049917	35.0	33.0	35.0	29.0	36.5
90-91	33.307819610412324	35.0	33.0	35.0	29.0	36.0
92-93	33.22072926697601	35.0	33.0	35.0	29.0	36.0
94-95	33.179198835254006	35.0	33.0	35.0	29.0	36.0
96-97	33.23225147126662	35.0	33.0	35.0	29.0	35.0
98-99	33.34221784989013	35.0	33.0	35.0	29.5	35.0
100	32.11604991643082	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	34.0
21	233.0
22	1179.0
23	4101.0
24	10766.0
25	22957.0
26	42119.0
27	66578.0
28	95795.0
29	127496.0
30	162515.0
31	201853.0
32	252214.0
33	320541.0
34	430675.0
35	602223.0
36	954945.0
37	1678216.0
38	2123517.0
39	1184810.0
40	45656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.523744571305755	20.249362725027407	13.710699375512553	24.516193328154287
2	15.092316318922053	21.044674686585587	40.50535334991109	23.35765564458127
3	18.37478372274246	25.532087080037897	30.53718407911215	25.55594511810749
4	12.752151365863673	17.61247918275113	38.12322370482792	31.512145746557273
5	13.294174292153505	37.631210204084084	35.762730790797306	13.311884712965108
6	30.83890961441712	37.45102018027408	18.272843741257354	13.437226464051447
7	26.989012334857833	32.25587535706009	22.742713812807455	18.01239849527462
8	24.801409149143357	36.60719967628953	21.014260791049452	17.577130383517662
9	25.33853319293072	16.58286135562165	21.088128254665044	36.99047719678259
10-11	24.477218395303797	26.575897257946572	28.695942692680372	20.250941654069255
12-13	24.887529822547588	23.895602172318192	28.939554033593367	22.277313971540856
14-15	22.066259787258822	25.54924517586148	26.186958406326283	26.19753663055342
16-17	22.061931240341817	28.77418072794032	26.929385250281268	22.234502781436596
18-19	22.003186675039593	27.992268650874173	28.376674835473192	21.62786983861304
20-21	22.684402792688736	27.25969023641477	28.208946952591262	21.846960018305232
22-23	22.473244679033723	27.25813179638713	28.05972411274496	22.20889941183418
24-25	22.297911988230762	27.277557488908684	28.273577875598527	22.150952647262027
26-27	22.333331911255364	27.49241046001238	28.112991265096383	22.061266363635873
28-29	22.1168178666256	27.475868633285433	28.13484664187436	22.272466858214614
30-31	22.493561600748144	27.391005161565555	28.084092664744087	22.031340572942213
32-33	22.411884856071904	27.41698353172196	28.123392968101435	22.047738644104705
34-35	22.17902097579418	27.49976457853851	28.269785526950336	22.05142891871697
36-37	22.334582249583022	27.47211020201523	28.05919148076778	22.134116067633965
38-39	22.074533403931937	27.53191350557148	28.188753055571365	22.20480003492522
40-41	22.322438025020393	27.349874524407024	28.15953147475854	22.168155975814035
42-43	22.288493600497063	27.39556628376956	28.0657148395932	22.250225276140178
44-45	22.210423842223975	27.645440566125473	27.894126781562097	22.250008810088463
46-47	22.461065609276943	27.396069159409826	27.628748920032383	22.514116311280855
48-49	22.299661475549733	27.492213194164417	27.720193990742636	22.487931339543213
50-51	22.14992046308074	27.648364357591444	27.763081702365817	22.438633476962
52-53	22.279686765623662	27.677332016015754	27.726351532381404	22.316629685979176
54-55	22.294796237247947	27.56365535954278	27.645738076991933	22.49581032621734
56-57	22.402555970620835	27.729384280418223	27.46483608865475	22.403223660306196
58-59	22.11373346487109	27.7615480761709	27.661484346087583	22.463234112870428
60-61	22.50447111321024	27.86023022810287	27.563288821195364	22.072009837491525
62-63	22.087160063867124	28.028578518843062	27.495222032398924	22.389039384890893
64-65	22.038780175746766	27.93646615673446	27.500975468360195	22.523778199158574
66-67	22.156450084560564	27.891737626240353	27.626891234907884	22.3249210542912
68-69	22.19308958084529	28.033714479421768	27.492351887955607	22.280844051777336
70-71	22.118247781736084	27.721724619408878	27.31950874751178	22.840518851343262
72-73	22.23974435809132	27.755597509682744	27.23458654899979	22.770071583226148
74-75	22.126332887865864	27.935755458246437	27.49925880298606	22.43865285090164
76-77	22.284816721597444	27.52664078359332	27.40815239624258	22.780390098566656
78-79	22.229253966444812	27.557761106894237	27.094680332518234	23.118304594142717
80-81	22.518202646678194	27.89593042542173	27.131153075354504	22.45471385254557
82-83	22.582793857210092	27.54523927023008	27.22438196848751	22.647584904072318
84-85	22.409456233606782	27.572558085915666	27.109172876533222	22.908812803944333
86-87	22.204864126426028	27.867903557238694	27.262739684249514	22.664492632085764
88-89	22.57480072316281	27.846402251574265	27.232919921224507	22.345877104038422
90-91	22.590976729546885	27.913983879358156	27.218060130091633	22.276979261003323
92-93	22.334920010520136	27.4986331314301	27.284596415170405	22.881850442879358
94-95	22.430795392904624	27.93553575379714	27.369493166661435	22.264175686636804
96-97	22.737545991440708	27.745404745268846	27.482988714966773	22.034060548323673
98-99	23.317128689283944	29.896596721140263	27.824179409657894	18.962095179917902
100	29.81084036315104	41.39433168689433	28.794827949954627	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1347.0
1	1193.5
2	1640.5
3	2396.5
4	3227.5
5	5101.0
6	6749.5
7	7822.0
8	8526.0
9	8424.0
10	7734.5
11	7064.5
12	7533.0
13	9658.0
14	15036.5
15	22473.5
16	28681.0
17	32913.5
18	34703.5
19	34634.0
20	33850.0
21	33235.5
22	34093.0
23	37745.0
24	44903.0
25	55617.0
26	70624.0
27	88135.0
28	105877.5
29	125518.0
30	143801.0
31	160471.0
32	179322.0
33	199030.0
34	218878.5
35	237861.0
36	259050.0
37	277818.5
38	289405.5
39	299439.0
40	313581.0
41	327764.5
42	336825.5
43	345369.0
44	354459.5
45	361167.0
46	366448.5
47	372300.5
48	376909.0
49	377154.5
50	372019.5
51	361135.0
52	344278.5
53	326331.0
54	307732.5
55	285347.0
56	260921.0
57	236215.5
58	211473.0
59	184380.5
60	155785.0
61	128868.0
62	103512.0
63	80455.5
64	61500.0
65	47278.5
66	36126.5
67	27842.0
68	21510.5
69	16278.0
70	12148.0
71	8781.0
72	6287.0
73	4471.0
74	3130.5
75	2109.5
76	1399.0
77	931.0
78	637.5
79	438.5
80	289.5
81	199.5
82	144.5
83	101.5
84	73.5
85	56.5
86	42.5
87	33.0
88	21.5
89	11.0
90	7.5
91	6.5
92	5.0
93	3.0
94	2.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005415384940510915
22-23	0.004641318542758072
24-25	0.0036573340989112493
26-27	0.016647557921606163
28-29	0.021061066511244852
30-31	0.03866966707946436
32-33	0.023431067334976872
34-35	0.012384276073196171
36-37	0.007063419424562283
38-39	0.0027685363063014844
40-41	0.008268873891454094
42-43	0.009868026932354959
44-45	0.006068097024418938
46-47	0.0020225332099631016
48-49	0.01014029208214693
50-51	0.004789005393501887
52-53	6.664787640871118E-6
54-55	4.1758490444040926E-4
56-57	5.995544901351144E-4
58-59	3.6464235121388066E-4
60-61	8.339614684782719E-4
62-63	7.669174604027851E-4
64-65	0.0
66-67	2.2394358586560553E-4
68-69	4.6888126614938805E-4
70-71	2.6743005069879475E-4
72-73	6.245921808132656E-4
74-75	2.819245664343049E-4
76-77	0.0
78-79	7.822706186978324E-6
80-81	3.887701597163025E-4
82-83	0.002357591799635917
84-85	3.263447422639367E-5
86-87	8.195610513792757E-4
88-89	2.943643441684363E-4
90-91	0.009010152979220346
92-93	7.145834989075587E-4
94-95	1.779364338104491E-5
96-97	2.379355031892051E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	195.0
20-21	623.0
22-23	1276.0
24-25	2813.0
26-27	5044.0
28-29	11341.0
30-31	26302.0
32-33	34135.0
34-35	47185.0
36-37	71225.0
38-39	77765.0
40-41	80337.0
42-43	85298.0
44-45	97603.0
46-47	96956.0
48-49	89655.0
50-51	79691.0
52-53	76934.0
54-55	83296.0
56-57	89192.0
58-59	54149.0
60-61	89618.0
62-63	88047.0
64-65	93388.0
66-67	97679.0
68-69	95121.0
70-71	88587.0
72-73	82238.0
74-75	84023.0
76-77	84693.0
78-79	89956.0
80-81	88783.0
82-83	85385.0
84-85	86861.0
86-87	93493.0
88-89	99698.0
90-91	105144.0
92-93	112676.0
94-95	136418.0
96-97	342844.0
98-99	1698396.0
100-101	3474367.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.9748595529364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.18755559487676	77.53862425692174
2	6.886489326956799	11.841299053958126
3	1.6861958502048222	4.3491135420031135
4	0.5963148502584092	2.050723420011882
5	0.26302981150121035	1.1306975551025948
6	0.13692120539596306	0.706306884224201
7	0.07064483444091982	0.4251575803438968
8	0.0429730605437289	0.29556822758455403
9	0.031086234828948917	0.2405371206103635
>10	0.09702807836987948	1.2371197680222836
>50	0.0013505259365632519	0.07565356870437963
>100	3.465314945208625E-4	0.05084204048262863
>500	2.1365082717907667E-5	0.013317234898565108
>1k	4.273010877330769E-5	0.04503974713172326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.602119487106213E-5	0.0	3.602119487106213E-5	0.0
3	0.0	3.602119487106213E-5	0.0	7.204238974212426E-5	0.0
4	0.0	1.200706495702071E-4	0.0	8.404945469914498E-5	1.2007064957020711E-5
5	0.0	1.3207771452722783E-4	0.0	1.080635846131864E-4	1.2007064957020711E-5
6	2.401412991404142E-4	1.3207771452722783E-4	0.0	1.200706495702071E-4	1.2007064957020711E-5
7	2.401412991404142E-4	1.3207771452722783E-4	0.0	1.200706495702071E-4	1.2007064957020711E-5
8	2.401412991404142E-4	1.4408477948424852E-4	0.0	1.200706495702071E-4	2.4014129914041423E-5
9	2.401412991404142E-4	2.041201042693521E-4	0.0	1.4408477948424852E-4	2.4014129914041423E-5
10-11	2.401412991404142E-4	2.041201042693521E-4	0.0	1.6809890939828995E-4	2.4014129914041423E-5
12-13	2.401412991404142E-4	2.041201042693521E-4	0.0	1.8010597435531067E-4	2.4014129914041423E-5
14-15	2.401412991404142E-4	3.001766239255178E-4	0.0	1.86109506833821E-4	5.4031792306593196E-5
16-17	3.241907538395592E-4	3.001766239255178E-4	0.0	1.9211303931233138E-4	8.404945469914498E-5
18-19	3.361978187965799E-4	3.241907538395592E-4	0.0	1.9811657179084175E-4	8.404945469914498E-5
20-21	3.4220135127509027E-4	3.4820488375360064E-4	0.0	2.4614483161892456E-4	8.404945469914498E-5
22-23	3.54208416232111E-4	4.2625080597423525E-4	0.0	3.121836888825385E-4	8.404945469914498E-5
24-25	3.6021194871062133E-4	5.283108581089113E-4	0.0	3.9022961110317313E-4	9.005298717765533E-5
26-27	3.6621548118913165E-4	5.643320529799735E-4	0.0	5.163037931518906E-4	9.605651965616569E-5
28-29	3.72219013667642E-4	5.643320529799735E-4	0.0	0.0012067100281805813	9.605651965616569E-5
30-31	3.72219013667642E-4	6.483815076791184E-4	0.0	0.003842260786246628	9.605651965616569E-5
32-33	3.72219013667642E-4	6.603885726361391E-4	0.0	0.009089348172464679	1.200706495702071E-4
34-35	4.082402085387042E-4	7.984698196418773E-4	0.0	0.015561156184298841	1.200706495702071E-4
36-37	4.082402085387042E-4	9.245440016905947E-4	0.0	0.027105949140474254	1.200706495702071E-4
38-39	4.082402085387042E-4	9.605651965616568E-4	0.0	0.05917081610819806	1.200706495702071E-4
40-41	4.202472734957249E-4	0.0010085934563897397	0.0	0.09312679580665263	1.4408477948424852E-4
42-43	4.202472734957249E-4	0.001044614651260802	0.0	0.11288442119343021	1.4408477948424852E-4
44-45	4.202472734957249E-4	0.001164685300831009	0.0	0.137396844303188	1.4408477948424852E-4
46-47	4.202472734957249E-4	0.0012607418204871746	0.0	0.16276777255737274	1.4408477948424852E-4
48-49	4.202472734957249E-4	0.0012607418204871746	0.0	0.18587536906715912	1.5609184444126924E-4
50-51	4.56268468366787E-4	0.0013868160025358922	0.0	0.20781227674363595	1.5609184444126924E-4
52-53	4.802825982808284E-4	0.0013928195350144026	0.0	0.266406753733897	1.6209537691977958E-4
54-55	4.802825982808284E-4	0.0014108301324499335	0.0	0.3352792783273678	1.6809890939828995E-4
56-57	4.802825982808284E-4	0.0014408477948424853	0.0	0.4020685771507956	1.6809890939828995E-4
58-59	4.8628613075933874E-4	0.0014588583922780164	0.0	0.6734402522444206	1.7410244187680032E-4
60-61	5.343143905874217E-4	0.0015248972495416303	0.0	0.915112452166855	1.86109506833821E-4
62-63	5.763391179369941E-4	0.0015669219768912027	0.0	0.9256726657965547	1.9211303931233138E-4
64-65	5.763391179369941E-4	0.0017290173538109825	0.0	0.935158247112601	1.9211303931233138E-4
66-67	5.823426504155045E-4	0.0017530314837250237	0.0	0.9439834398560113	1.9211303931233138E-4
68-69	5.883461828940148E-4	0.0018190703409886378	0.0	0.951908102727645	1.9211303931233138E-4
70-71	6.063567803295459E-4	0.0018370809384241687	0.0	0.9581157553104247	1.9211303931233138E-4
72-73	6.363744427220977E-4	0.001843084470902679	0.0	0.9631527190598949	2.041201042693521E-4
74-75	6.423779752006081E-4	0.0018851091982522517	0.0	0.9669409480538349	2.041201042693521E-4
76-77	6.483815076791184E-4	0.0019691586529513964	0.0	0.9709032794896517	2.041201042693521E-4
78-79	6.483815076791184E-4	0.001993172782865438	0.0	0.9736348867673739	2.041201042693521E-4
80-81	6.483815076791184E-4	0.001993172782865438	0.0	0.9743913318596662	2.161271692263728E-4
82-83	6.483815076791184E-4	0.001999176315343948	0.0	0.9749436568476892	2.281342341833935E-4
84-85	6.483815076791184E-4	0.0020231904452579896	0.0	0.9751057522246089	2.281342341833935E-4
86-87	6.483815076791184E-4	0.0020291939777365	0.0	0.9752318264066577	2.281342341833935E-4
88	6.483815076791184E-4	0.0021132434324356453	0.0	0.9752378299391362	2.281342341833935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8245	0.0	42.90706	1
GTATCAA	13590	0.0	32.908943	1
TCAACGC	15740	0.0	27.55431	4
ATCAACG	15780	0.0	27.458485	3
CAACGCA	16235	0.0	26.739437	5
AACGCAG	16760	0.0	25.926292	6
TATCAAC	17205	0.0	25.80372	2
ACGCAGA	19895	0.0	21.820293	7
CGCAGAG	20185	0.0	21.445873	8
TACATGG	21005	0.0	21.116074	2
GTACATG	21710	0.0	20.90241	1
ACATGGG	21600	0.0	20.021992	3
CATGGGG	16725	0.0	18.284424	4
AGACCGA	13665	0.0	18.197962	88-89
CTCTTAT	12405	0.0	18.078653	62-63
ACCGAGG	14165	0.0	17.945017	90-91
GACCGAG	13900	0.0	17.793491	90-91
CGAGACC	13685	0.0	17.753069	86-87
GCAGAGT	24510	0.0	17.561216	9
GAGACCG	13890	0.0	17.48835	88-89
>>END_MODULE