FastQCFastQC Report
Fri 27 May 2016
SRR2140204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9568566
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92000.045.5749441
GTATCAA158300.033.630171
TCAACGC185900.027.7270264
ATCAACG188400.027.4248143
CAACGCA191900.026.9031165
CGAGACC89900.026.25076586-87
AACGCAG197700.026.218226
AGACCGA91800.025.9457188-89
TATCAAC201600.025.8747862
ACCGAGG93950.025.76051590-91
GACCGAG91850.025.51033290-91
GAGACCG90600.025.26424288-89
ACGAGAC90300.025.25683286-87
CACGAGA93450.024.92061884-85
CCCACGA90850.024.76168482-83
GCCCACG90250.024.36122582-83
TCTCCGA94350.023.11032374-75
CCACGAG98000.022.82245884-85
ATCTCCG93950.022.55374774-75
ACGCAGA232900.022.1847787
Produced by FastQC (version 0.11.5)