FastQCFastQC Report
Fri 27 May 2016
SRR2140203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7716155
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82950.042.9364471
CTTATAC73450.035.3267021
GTATCAA136700.032.1594351
TATACAC100150.028.713993
TCAACGC158900.026.9903624
ATCAACG159050.026.8609963
CAACGCA163300.026.1872225
AACGCAG169500.025.2537176
TATCAAC174550.024.6177712
TTATACA115650.023.8295292
ACATCTC119850.022.2360138
ACACATC120850.021.915266
ACGCAGA197600.021.7461157
TACACAT135500.021.436375
CGCAGAG201400.021.335818
TACATGG200450.020.3650822
GTACATG207850.020.3357811
CACATCT132850.019.7491057
ACATGGG202450.019.2863163
CATCTCC141150.018.7048939
Produced by FastQC (version 0.11.5)